BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0030.0130.p
         (234 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogen...   230   9e-62
gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen...   126   2e-30
gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenas...   108   3e-25

>gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 288

 Score =  230 bits (586), Expect = 9e-62,   Method: Compositional matrix adjust.
 Identities = 115/183 (62%), Positives = 141/183 (77%)

Query: 1   MKHIGVAGLGNMGRGMALSLQRGGFTVSGYDPASAAVESIAREGVNTFASVAALAAEVDV 60
           MK IGV GLGNMGRGMA SLQRGG+ V GYD   +   ++A++G++   S+AALA  V++
Sbjct: 1   MKKIGVIGLGNMGRGMAFSLQRGGYEVLGYDAQESTRTALAQQGLHCVDSIAALAPAVEM 60

Query: 61  LILSLPTSDVVEAVVFGADGVLASAKAGLLVVDTTTADPNSTRKVAAALAEKGVRFIDAP 120
           ++LSLPTSD+VE VV G  G+L     GL++VDTTTADPNSTR++AA L   G+  +DAP
Sbjct: 61  VVLSLPTSDIVEQVVEGEGGLLQHGAPGLVIVDTTTADPNSTRRLAARLQAAGMELVDAP 120

Query: 121 VSGGPKGAITGTMTMVLGGAAEDVAAVTPVLEAMSAKRVHVGPVGAGHVTKLLNNLMCGA 180
           VSGGPKGA T TM MV+GG+AE VA + PVL  MSAKRVHVGPVGAGHV KLLNNL+ G 
Sbjct: 121 VSGGPKGAATATMGMVIGGSAETVARIEPVLAVMSAKRVHVGPVGAGHVAKLLNNLVTGT 180

Query: 181 HLI 183
           HL+
Sbjct: 181 HLL 183


>gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 297

 Score =  126 bits (316), Expect = 2e-30,   Method: Compositional matrix adjust.
 Identities = 79/185 (42%), Positives = 109/185 (58%)

Query: 3   HIGVAGLGNMGRGMALSLQRGGFTVSGYDPASAAVESIAREGVNTFASVAALAAEVDVLI 62
           +I   GLGNMG  MA +L + GF+VSG+D   A+V+ +   G  T A   A  A+ DV+I
Sbjct: 4   NIVFIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVAKADVVI 63

Query: 63  LSLPTSDVVEAVVFGADGVLASAKAGLLVVDTTTADPNSTRKVAAALAEKGVRFIDAPVS 122
             LP S  VE++  G  GVLASAK G L++D +T  P + RKVAAA   +G   +DAPVS
Sbjct: 64  TMLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDAPVS 123

Query: 123 GGPKGAITGTMTMVLGGAAEDVAAVTPVLEAMSAKRVHVGPVGAGHVTKLLNNLMCGAHL 182
           GG  GA  GT+T ++GG+ E   A  P L+ M     H G  G+G   K+ NN++ G  +
Sbjct: 124 GGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNMLLGILM 183

Query: 183 IAAAK 187
           I  ++
Sbjct: 184 IGTSE 188


>gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 298

 Score =  108 bits (271), Expect = 3e-25,   Method: Compositional matrix adjust.
 Identities = 71/190 (37%), Positives = 106/190 (55%), Gaps = 1/190 (0%)

Query: 2   KHIGVAGLGNMGRGMALSLQRGGFTVSGYDPASAAVESIAREGVNTFASVAALAAEVDVL 61
           +++GV GLG MG G+A SL R GF V   D     ++  A  G    A+ A LA +VDV+
Sbjct: 3   RNVGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAGKVDVV 62

Query: 62  ILSLPTSDVVEAVVFGADGVLASAKAGLLVVDTTTADPNSTRKVAAALAEKGVRFIDAPV 121
           I  +  +   E V+FG +G +++ K G +V+ + T  P+   ++   L EKG+  +DAPV
Sbjct: 63  ITLVVNAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRLVEKGLLLLDAPV 122

Query: 122 SGGPKGAITGTMTMVLGGAAEDVAAVTPVLEAMSAKRVHVGPV-GAGHVTKLLNNLMCGA 180
           SGG   A +G MTM+  G AE  A +  VL  M+ K   +G   G G   K++N L+ G 
Sbjct: 123 SGGAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGV 182

Query: 181 HLIAAAKRRA 190
           H+ A+A+  A
Sbjct: 183 HIAASAEAMA 192


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.314    0.127    0.349 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 731,579
Number of extensions: 29670
Number of successful extensions: 152
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 152
Number of HSP's successfully gapped: 4
Length of query: 234
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 152
Effective length of database: 1,231,600
Effective search space: 187203200
Effective search space used: 187203200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 129 (54.3 bits)