BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0127.p (378 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 559 e-160 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 301 5e-83 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 279 2e-76 gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrog... 265 6e-72 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 263 2e-71 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 559 bits (1440), Expect = e-160, Method: Compositional matrix adjust. Identities = 271/377 (71%), Positives = 311/377 (82%) Query: 1 MFEYYAGWTDKFYGDVIPVPSSHLNYTRREPVGAVLQITPWNAPIFTCGWQVAPAIAMGN 60 MFEYYAGW DKFYG+VIPVPSSHLNYTRREP G VLQ+TPWNAP FTCGWQ+ PA+A GN Sbjct: 126 MFEYYAGWADKFYGEVIPVPSSHLNYTRREPYGTVLQMTPWNAPAFTCGWQLGPALATGN 185 Query: 61 AVLLKPSELTPFTSLAVAALAEQAGVPKGLINVLAGQGHTVGQAAIDHRVVKKVVFVGSP 120 AVLLKPSELTPF+SLA+A L EQAG+P GL+NVLAG G T+ A+ VKKV+FVGSP Sbjct: 186 AVLLKPSELTPFSSLAIARLGEQAGLPAGLVNVLAGLGQTMVPQAMATWTVKKVIFVGSP 245 Query: 121 QTGKKIAEAAARRLLPCVLELGGKSANIVFADADLKRACLGAQAAIFSSAGQSCVAGSRL 180 TG IA+AAA R++PCVLELGGKSANI+F DADL+ A GAQAAIFS AGQSCVAGSRL Sbjct: 246 ATGALIAKAAAARVMPCVLELGGKSANIIFEDADLRLAAFGAQAAIFSGAGQSCVAGSRL 305 Query: 181 LVQRSVYEEFVAMLANGASKIKVGQPLDAQTEVGPISNRKQYEHVRGMIAEGVRNGARLA 240 LVQR VY+ FV +A GA KI++G PLD TEVGPI+NRKQYEH++ M+A G+ GA LA Sbjct: 306 LVQRKVYDRFVETVAAGAEKIRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLEAGATLA 365 Query: 241 TVAQDFSTPGFFVRPTVLADVSNDMAVAQQEIFGPVVAAIPFDTEEDAVRIANDSDFGLA 300 + G+FVRPT+LA SN M VA+ EIFGPV AIPF+ EE+A+ IANDS+FGLA Sbjct: 366 AGHTRYGEEGYFVRPTLLAHASNAMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLA 425 Query: 301 GAVWTNDVARAHRVAASINAGTFWINGYKTINVTSPFGGYGQSGYGRSSGVEALYEYTQT 360 GAVWT DVARAHRVAAS+NAGTFW+N YKTINV SPFGG+ +SGYGRSSG+EALY+YTQT Sbjct: 426 GAVWTRDVARAHRVAASVNAGTFWVNSYKTINVASPFGGFNRSGYGRSSGMEALYDYTQT 485 Query: 361 KSVWVETAKEPAAAFGY 377 KSVWVET+ PA FGY Sbjct: 486 KSVWVETSASPATPFGY 502 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 301 bits (772), Expect = 5e-83, Method: Compositional matrix adjust. Identities = 158/364 (43%), Positives = 221/364 (60%), Gaps = 4/364 (1%) Query: 6 AGWTDKFYGDVIP----VPSSHLNYTRREPVGAVLQITPWNAPIFTCGWQVAPAIAMGNA 61 AGW ++ G IP + L+YT REP+G V I PWNAP+ W+VA +A G Sbjct: 117 AGWANQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCT 176 Query: 62 VLLKPSELTPFTSLAVAALAEQAGVPKGLINVLAGQGHTVGQAAIDHRVVKKVVFVGSPQ 121 V++KP+E TP ++L +A LA++AG P G+INV+ G G++VG+A +H V K+ F GSP+ Sbjct: 177 VVIKPAEETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPE 236 Query: 122 TGKKIAEAAARRLLPCVLELGGKSANIVFADADLKRACLGAQAAIFSSAGQSCVAGSRLL 181 G+ I A + LELGGKS IVF DA + A G +F + GQ C AGSR+L Sbjct: 237 AGRAIQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRIL 296 Query: 182 VQRSVYEEFVAMLANGASKIKVGQPLDAQTEVGPISNRKQYEHVRGMIAEGVRNGARLAT 241 VQRS+ + F LA A+ I VG P +GP++ + Q+E V I +G+ GA L Sbjct: 297 VQRSLAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLA 356 Query: 242 VAQDFSTPGFFVRPTVLADVSNDMAVAQQEIFGPVVAAIPFDTEEDAVRIANDSDFGLAG 301 G+FV+PT+ AD +N M++A++EIFGPV I FDTEE+A+ +ANDS +GLA Sbjct: 357 GGVSRPDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAA 416 Query: 302 AVWTNDVARAHRVAASINAGTFWINGYKTINVTSPFGGYGQSGYGRSSGVEALYEYTQTK 361 VWT ++ARAHRVAA + G +N + ++ P+GG SG GR G+ YT+ K Sbjct: 417 TVWTTNLARAHRVAAGVKVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEK 476 Query: 362 SVWV 365 +V V Sbjct: 477 TVTV 480 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 279 bits (714), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 153/369 (41%), Positives = 211/369 (57%), Gaps = 5/369 (1%) Query: 2 FEYYAGWTDKFYGDVIPVPSSHLNYTRREPVGAVLQITPWNAPIFTCGWQVAPAIAMGNA 61 FE+YAG DK +GD IP + + T REP G I PWN P+ G V A+A GN Sbjct: 108 FEFYAGAADKLHGDTIPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNT 167 Query: 62 VLLKPSELTPFTSLAVAALAEQAGVPKGLINVLAGQGHTVGQAAIDHRVVKKVVFVGSPQ 121 ++KP+E + L VA LA + G P+G +N++ G GH G + H V + F GSP Sbjct: 168 CVVKPAEDACLSILRVAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPN 227 Query: 122 TGKKIAEAAARRLLPCVLELGGKSANIVFADADLKRACLGAQAAIFSSAGQSCVAGSRLL 181 GK + AAA P LELGGKS IVF DAD++ AI +AGQ+C AGSRLL Sbjct: 228 VGKLVVRAAAEHHTPVTLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLL 287 Query: 182 VQRSVYEEFVAMLANGASKIKVGQPLDAQTEVGPISNRKQYEHVRGMIAEGVRNGARLA- 240 VQR YE+ + L+ S+++VG + GP+ + Q E V G +++ R+G LA Sbjct: 288 VQRGAYEQVLERLSGLFSQLQVGSS-QMDLDCGPLIRKTQQERVAGFLSDAQRDGIALAA 346 Query: 241 --TVAQDFSTPGFFVRPTVLADVSNDMAVAQQEIFGPVVAAIPFDTEEDAVRIANDSDFG 298 +A T GF+ P +L DV +AQ+E+FGPV+AA+PF+ E A+ +AN +D+G Sbjct: 347 QGKIAAGVPTGGFYQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYG 406 Query: 299 LAGAVWTNDVARAHRVAASINAGTFWINGYKT-INVTSPFGGYGQSGYGRSSGVEALYEY 357 L +VWT D R R+A +I +G +IN Y V PFGG SG+GR G EALY + Sbjct: 407 LVASVWTRDGGRQMRLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGF 466 Query: 358 TQTKSVWVE 366 T K++ + Sbjct: 467 TVLKTIAIR 475 >gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 793 Score = 265 bits (676), Expect = 6e-72, Method: Compositional matrix adjust. Identities = 152/372 (40%), Positives = 214/372 (57%), Gaps = 11/372 (2%) Query: 2 FEYYAGWTDKFYGDVIPVPSSHLNYTRREPVGAVLQITPWNAPIFTCGWQVAPAIAMGNA 61 F +YAGW + PVG V QI PWN P+ W++APA+A+GN Sbjct: 137 FYHYAGWAQL----------QESEFPDHVPVGVVGQIVPWNFPLLMLAWKIAPALALGNT 186 Query: 62 VLLKPSELTPFTSLAVAALAEQAGVPKGLINVLAGQGHTVGQAAIDHRVVKKVVFVGSPQ 121 V+LKP+E T T+L A LA+QAG+P G++N++ G G T G A + H ++K+ F GS + Sbjct: 187 VVLKPAENTSLTALLFAELAQQAGLPAGVLNIVTGDGAT-GAAVVAHPGIQKIAFTGSTE 245 Query: 122 TGKKIAEAAARRLLPCVLELGGKSANIVFADADLKRACLGAQAAIFSSAGQSCVAGSRLL 181 G+ I E A LELGGKS IVF DAD+ A G AI+ + GQ C AGSRLL Sbjct: 246 VGRLIREQTAGSGKSLTLELGGKSPFIVFEDADIDAAIEGVVDAIWFNQGQVCCAGSRLL 305 Query: 182 VQRSVYEEFVAMLANGASKIKVGQPLDAQTEVGPISNRKQYEHVRGMIAEGVRNGARLAT 241 VQ +++ F+A L K+KVG PLD +++G + + Q E VR ++ +GVR GA+ Sbjct: 306 VQEGIHDLFIARLKTRMQKLKVGAPLDKCSDMGALISPVQLERVRSLVEQGVREGAQCHQ 365 Query: 242 VAQDFSTPGFFVRPTVLADVSNDMAVAQQEIFGPVVAAIPFDTEEDAVRIANDSDFGLAG 301 V D G F PT+L V VA +EIFGPV+ A+ F T ++AV++AN+S +GLA Sbjct: 366 VVLDTPPGGCFYPPTLLTGVHPAATVASEEIFGPVLVAMSFRTPDEAVQLANNSRYGLAA 425 Query: 302 AVWTNDVARAHRVAASINAGTFWINGYKTINVTSPFGGYGQSGYGRSSGVEALYEYTQTK 361 ++W+ + A VA + AG WIN + FGG +SGYGR G E YEY + + Sbjct: 426 SIWSETIGLALGVAPQLKAGVVWINSTNQFDAAVGFGGVRESGYGREGGREGCYEYLKPR 485 Query: 362 SVWVETAKEPAA 373 + ++P+A Sbjct: 486 RQQAGSLRQPSA 497 Score = 58.5 bits (140), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 37/138 (26%), Positives = 61/138 (44%) Query: 5 YAGWTDKFYGDVIPVPSSHLNYTRREPVGAVLQITPWNAPIFTCGWQVAPAIAMGNAVLL 64 Y W DKF G V P + E +G V + P P+ VAP IAMGN V++ Sbjct: 624 YGAWADKFEGAVHQPPMRGVALAMPEAIGVVGVVCPEEKPLLALISLVAPLIAMGNRVVV 683 Query: 65 KPSELTPFTSLAVAALAEQAGVPKGLINVLAGQGHTVGQAAIDHRVVKKVVFVGSPQTGK 124 PSE P + + + + + +P G+IN++ G+ + +H V+ + G + Sbjct: 684 VPSEQAPLVATDLYQVLDTSDLPAGVINIVTGKSAELLPVLAEHDEVEALWVRGPAEFSA 743 Query: 125 KIAEAAARRLLPCVLELG 142 + L C ++ G Sbjct: 744 TAERLSTGNLKRCFVDHG 761 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 263 bits (671), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 149/342 (43%), Positives = 207/342 (60%), Gaps = 12/342 (3%) Query: 29 REPVGAVLQITPWNAPIFTCGWQVAPAIAMGNAVLLKPSELTPFTSLAVAALAEQAGVPK 88 REP+G ITPWN PI+ +V PA+A G V+LKPSEL+P ++L A + +AGVP Sbjct: 145 REPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSALLFAEIVHEAGVPA 204 Query: 89 GLINVLAGQGHTVGQAAIDHRVVKKVVFVGSPQTGKKIAEAAARRLLPCVLELGGKSANI 148 G+ N++ G G VG A H V + GS + G +A+AAA + ELGGKS N+ Sbjct: 205 GVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVKRVAQELGGKSPNL 264 Query: 149 VFADADLKRACLGAQAAIFSSAGQSCVAGSRLLVQRS----VYEEFVAMLANGASKIKVG 204 V DADL+RA AA + GQSC A +RL+V R+ V+E +A LA ++KVG Sbjct: 265 VLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELALATLA----QMKVG 320 Query: 205 QPLDAQTEVGPISNRKQYEHVRGMIAEGVRNGARLATVA---QDFSTPGFFVRPTVLADV 261 P DA T GP++NR Q+ V+ MI G+++GARL D G++ RPT+ ++V Sbjct: 321 DPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGYYARPTIFSEV 380 Query: 262 SNDMAVAQQEIFGPVVAAIPFDTEEDAVRIANDSDFGLAGAVWTNDVARAHRVAASINAG 321 DM +AQ+EIFGPV+A +P+DT E+A+ IAND+ +GL V D + VAA I +G Sbjct: 381 HTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVRAVAARIQSG 440 Query: 322 TFWINGYKTINVTSPFGGYGQSGYGRSSGVEALYEYTQTKSV 363 +N Y + +PFGGY QSG GR GVE + EY + K++ Sbjct: 441 QVHLN-YPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVKAI 481 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,166,228 Number of extensions: 48440 Number of successful extensions: 251 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 214 Number of HSP's successfully gapped: 20 Length of query: 378 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 291 Effective length of database: 1,207,925 Effective search space: 351506175 Effective search space used: 351506175 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)