BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0119.p (464 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 228 8e-61 gi|300311543|ref|YP_003775635.1| major facilitator superfamily p... 218 1e-57 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 211 1e-55 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 209 3e-55 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 198 6e-52 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 228 bits (581), Expect = 8e-61, Method: Compositional matrix adjust. Identities = 146/430 (33%), Positives = 235/430 (54%), Gaps = 11/430 (2%) Query: 40 ERQRVIALRSSVVGTALEWYDFFLYGTAAAIVFNVLFFTKLDPMIGTLAAFGSYALGFVV 99 +Q A S +G+ALE+YDFF+Y TAA+++F LFF K DP +A+ +Y +G+V Sbjct: 10 HQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVGYVA 69 Query: 100 RPLGGFVFGHIGDRIGRKNVLVITLLMMGLSTTLIGFLPTYASIGIAAPILLTLLRLIQG 159 RP+G FV GH+GD GRK VL+ + +MG ST +G LPTY +G+ AP LL ++RL QG Sbjct: 70 RPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVIMRLAQG 129 Query: 160 FAAGAEFGGAVTMSAEYAPVNKRGYYASWPGVGVALGVVLSAGVF-TLVRQMPEADFMNY 218 FA E GA +M E++P +RGY++S+ GV G +L+A VF L MP F + Sbjct: 130 FAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHYMPAEQFNVW 189 Query: 219 GWRIPFILSSIGLAVGMYIRLYVLESPAFEKIKSKKAVQKSPVSAVWKHHRRHLFIAIGA 278 GWRIPF+LS + + G IR V E+P F+ ++ ++SPV ++ ++ + Sbjct: 190 GWRIPFLLSFLVIIAGWVIRRTVDETPVFKAADKEELKRRSPVVDAFRDCSPNMLRVVCM 249 Query: 279 RFSENTAGYIFQVWALSYLTAQ---LKIDASIGLVAVLIGAGLGALLNPFWGMLSDRIGR 335 N + ++ +Y + + L ++G + L+ P+ G LSDRIGR Sbjct: 250 ALM-NVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVGKLSDRIGR 308 Query: 336 KPVYILGILVMILGSWPFFMMLETRETPMIIAGFVMM--IVFGLYPMFSVQAAFFAELFP 393 +P I+G L+ L ++ + + R + ++M +V+ Y +V +F+ ELFP Sbjct: 309 RPPIIVGSLLAGLFAFAYLYAISIRNVELAFVMSILMWGVVYQGYN--AVFPSFYPELFP 366 Query: 394 PSVRMSGICMARELSSVITGGMAPVIATGLLAYYGGSYYPIIGYLVF-MGGVALIALLMA 452 R+S + +A+ L + IT M P + T + + + +G + F + V+ +A L A Sbjct: 367 ARARVSAMAIAQNLGTAIT-AMLPALFTAVAPPGSSNIWLTVGSIAFAITIVSALAALSA 425 Query: 453 PETRGVIYEE 462 ET + E+ Sbjct: 426 RETYRIPLEK 435 >gi|300311543|ref|YP_003775635.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 553 Score = 218 bits (554), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 133/336 (39%), Positives = 199/336 (59%), Gaps = 7/336 (2%) Query: 36 GVGLERQRVIALRSSVVGTALEWYDFFLYGTAAAIVFNVLFFTKLDPMIGTLAAFGSYAL 95 G+ E ++VI +S +GT EWYDF+LYG+ AAI+ FF DP + A ++A Sbjct: 12 GITPEEKKVI--FASSLGTVFEWYDFYLYGSLAAIIAK-QFFAGTDPNTAFIFALLAFAA 68 Query: 96 GFVVRPLGGFVFGHIGDRIGRKNVLVITLLMMGLSTTLIGFLPTYASIGIAAPILLTLLR 155 GF+VRP G VFG +GD +GRK ++T+L+MG ST ++G LP Y SIGI API+L +LR Sbjct: 69 GFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITAPIILIILR 128 Query: 156 LIQGFAAGAEFGGAVTMSAEYAPVNKRGYYASWPGVGVALGVVLSAGVFTLVRQ-MPEAD 214 ++QG A G E+GGA T AE+AP +RG + +W +G+ LS V VR + EA Sbjct: 129 ILQGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGVRTAVGEAA 188 Query: 215 FMNYGWRIPFILSSIGLAVGMYIRLYVLESPAFEKIKSKKAVQKSPVSAV---WKHHRRH 271 F ++GWRIPF++S + LA+ ++IR+ + ESPAF K+K++ K+P++ WK+ + Sbjct: 189 FADWGWRIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFGQWKNLKLV 248 Query: 272 LFIAIGARFSENTAGYIFQVWALSYLTAQLKIDASIGLVAVLIGAGLGALLNPFWGMLSD 331 + +G + Y Q +AL +LT LK+D + + L F+G LSD Sbjct: 249 ILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGPTANILIAAALLLATPFFLFFGSLSD 308 Query: 332 RIGRKPVYILGILVMILGSWPFFMMLETRETPMIIA 367 +IGRKP+ + G L+ + +P F L P + A Sbjct: 309 KIGRKPIILGGCLIAAVTYFPIFSGLTHFANPALEA 344 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 211 bits (536), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 136/402 (33%), Positives = 216/402 (53%), Gaps = 19/402 (4%) Query: 40 ERQRVIALRSSVVGTALEWYDFFLYGTAAAIVFNVLFFTKLDPMIGTLAAFGSYALGFVV 99 +QR A+ ++V+G LEW+DF +Y A I+ LFF + + L A ++ +GF + Sbjct: 16 NQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAK-LFFPTGNDLTSLLLAVATFGVGFFM 74 Query: 100 RPLGGFVFGHIGDRIGRKNVLVITLLMMGLSTTLIGFLPTYASIGIAAPILLTLLRLIQG 159 RP+GG + G DR+GRK L +T+L+M + TT+IG PTY SIG+ AP+++ + RL+QG Sbjct: 75 RPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQG 134 Query: 160 FAAGAEFGGAVTMSAEYAPVNKRGYYASWPGVGVALGVVLSAGVFTLVR-QMPEADFMNY 218 F+AG E G A EYAP +R YY+SW + + V+L A V T V + A ++ Sbjct: 135 FSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASW 194 Query: 219 GWRIPFILSSIGLAVGMYIRLYVLESPAFEKIKSKKAVQKSPVSAVWKHHRRHLFIAIGA 278 GWR+PF+L I VG YIR ++ E+P F + K K SP+ V ++ R + Sbjct: 195 GWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKT---DSPLKEVIRNFPRETSASFSM 251 Query: 279 RFSENTAGYIFQVWALSYLTAQLKIDASIGLVAVLIGAGLGALLNPFWGMLSDRIGRKPV 338 Y+ + +Y LK+ + G +A ++G +L P G L+DRIGR+P+ Sbjct: 252 VILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRPL 311 Query: 339 YILGILVMILGSWPFFMMLETRETPMIIAGFVMMIVFGLYPMFSVQAAF-------FAEL 391 ++++ ++P F + + P + + +VFG ++ AA+ F+EL Sbjct: 312 LSGSAALILMLAYPMFAYINS--APSLSTLLIFQLVFG-----TLIAAYTGPILAAFSEL 364 Query: 392 FPPSVRMSGICMARELSSVITGGMAPVIATGLLAYYGGSYYP 433 FP V +G+ +A L+ I GG A T L+A G + P Sbjct: 365 FPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAP 406 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 209 bits (533), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 132/398 (33%), Positives = 212/398 (53%), Gaps = 8/398 (2%) Query: 39 LERQRVIALRSSVVGTALEWYDFFLYGTAAAIVFNVLFFTKLDPMIGTLAAFGSYALGFV 98 L +QR + +++VG ALEWYDF +YG +AI+ LFF + L A ++ +GF Sbjct: 5 LNKQRQVI--AAIVGNALEWYDFIVYGFFSAIIAR-LFFPADNEYTSLLVALATFGIGFF 61 Query: 99 VRPLGGFVFGHIGDRIGRKNVLVITLLMMGLSTTLIGFLPTYASIGIAAPILLTLLRLIQ 158 +RP+GG + G DR GRK + + +++M L+ +I F PTYA+IGIAAPI++ + R++Q Sbjct: 62 MRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQ 121 Query: 159 GFAAGAEFGGAVTMSAEYAPVNKRGYYASWPGVGVALGVVLSAGVFTLV-RQMPEADFMN 217 GFA G E+ + E AP +KRG Y SW VG L V AG+ LV R + + Sbjct: 122 GFATGGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDS 181 Query: 218 YGWRIPFILSSIGLAVGMYIRLYVLESPAFEKIKSKKAVQKSPVSAVWKHHRRHLFIAIG 277 +GWR+PF+L + VG++IR ++ E+ F + +K + V K + R + +++G Sbjct: 182 WGWRLPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMG 241 Query: 278 ARFSENTAGYIFQVWALSYLTAQLKIDASIGLVAVLIGAGLGALLNPFWGMLSDRIGRKP 337 + A Y+ V ++ QL + + +I L + PF G LSDR+GR+ Sbjct: 242 QVINGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRT 301 Query: 338 VYILGILVMILGSWPFFMMLETRET--PMIIAGFVMMIVFGLYPMFSVQAAFFAELFPPS 395 V + L +L +P F+ + + +++ ++ IV G F +E FP Sbjct: 302 VLLYSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGAN--FGPMPTALSEQFPTR 359 Query: 396 VRMSGICMARELSSVITGGMAPVIATGLLAYYGGSYYP 433 VR +G+ +A L++++ GG AP I T L G P Sbjct: 360 VRSTGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAP 397 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 198 bits (504), Expect = 6e-52, Method: Compositional matrix adjust. Identities = 135/412 (32%), Positives = 223/412 (54%), Gaps = 16/412 (3%) Query: 46 ALRSSVVGTALEWYDFFLYGTAAAIVFNVLFFTKLDPMIGTLAAFGSYALGFVVRPLGGF 105 A+ SS +G ALEW+D +YG A A+V FF D + L F ++ + F +RPLG Sbjct: 20 AIISSSIGNALEWFDILIYG-AFAVVIAKQFFPTGDDSVSLLLTFATFGVSFFMRPLGAV 78 Query: 106 VFGHIGDRIGRKNVLVITLLMMGLSTTLIGFLPTYASIGIAAPILLTLLRLIQGFAAGAE 165 V G DR GRK L++++++M + T +I F+P+YASIG+ AP + L ++IQGF+AG E Sbjct: 79 VLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFSAGGE 138 Query: 166 FGGAVTMSAEYAPVNKRGYYASWPGVGVALGVVLSAGVFTLVRQM--PEADFMNYGWRIP 223 FG + E+AP ++RG+++SW + ++L+A ++ M PE ++GWR+P Sbjct: 139 FGSSTAFLVEHAP-HRRGFFSSWQVASQGISLLLAALFGAVLNNMLTPE-QLASWGWRVP 196 Query: 224 FILSSIGLAVGMYIRLYVLESPAFEKIKSKKAVQKSPVSAVWKHHRRHLFIAIGARFSEN 283 FI + G+YIR ++ E+P F++ K +P+ + H + L I G+ Sbjct: 197 FIFGLLIAPAGIYIRRHLDEAPEFKESSEKT---NAPLRDTFAHQKMRLLIGAGSVIMAT 253 Query: 284 TAGYIFQVWALSYLTAQLKIDASIGLVAVLIGAGLGALLNPFWGMLSDRIGRKPVYILGI 343 + Y+ ++ +Y QL + A A+ + + + +P G LSD+IGR P I Sbjct: 254 VSVYL-SLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFMITSS 312 Query: 344 LVMILGSWPFFMMLETRETPMIIAGFVMMIVFG-LYPM-FSVQAAFFAELFPPSVRMSGI 401 + I+ ++P F+ L +P + ++ V G L M F+ A A++FP + R +G+ Sbjct: 313 ALYIVLTYPMFVFLT--NSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTGM 370 Query: 402 CMARELSSVITGGMAPVIATGLLAYYGGSYYPIIGYLVFMGGV-ALIALLMA 452 +A ++ I GG A +I T L+ + G + Y V G V +LIA L A Sbjct: 371 SLAYNIAVTIFGGFAGLIITWLIDFTGDKLS--VSYFVIFGAVLSLIASLSA 420 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.142 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,212,621 Number of extensions: 44023 Number of successful extensions: 321 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 268 Number of HSP's successfully gapped: 17 Length of query: 464 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 375 Effective length of database: 1,198,455 Effective search space: 449420625 Effective search space used: 449420625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)