BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0112.p (233 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310552|ref|YP_003774644.1| MotA/TolQ/ExbB proton channel f... 392 e-110 gi|300309940|ref|YP_003774032.1| biopolymer transport transmembr... 92 4e-20 gi|300310025|ref|YP_003774117.1| biopolymer transport transmembr... 90 2e-19 gi|300312127|ref|YP_003776219.1| biopolymer transport transmembr... 84 9e-18 gi|300310500|ref|YP_003774592.1| biopolymer transport ExbB-like ... 55 5e-09 >gi|300310552|ref|YP_003774644.1| MotA/TolQ/ExbB proton channel family protein [Herbaspirillum seropedicae SmR1] Length = 234 Score = 392 bits (1007), Expect = e-110, Method: Compositional matrix adjust. Identities = 206/234 (88%), Positives = 215/234 (91%), Gaps = 1/234 (0%) Query: 1 MNVTQDLSFFALITNASVIVQFVMLLLLLASVFSWTYIFRKMFTIRSARSQTEEFERSFW 60 MNVTQDLSF +LI NASV+VQ VMLLLL AS+ SWTYIFRKMFTIR+AR QTEEFER FW Sbjct: 1 MNVTQDLSFISLIANASVLVQLVMLLLLSASIISWTYIFRKMFTIRAARVQTEEFERVFW 60 Query: 61 SGGNLIALYQDTLSNRRKGGGHGGALERIFQAGMGEFNKAKAQVG-KGATIDSSGLLDGA 119 SGGNL ALYQD LSNRRK GG GGALERIFQAGMGEF KAKA V +G +D S LLDGA Sbjct: 61 SGGNLSALYQDALSNRRKAGGAGGALERIFQAGMGEFTKAKASVAARGGAVDPSLLLDGA 120 Query: 120 RRAMRAAYQREMDALESHLAFLATVGSVSPYVGLFGTVWGIMNAFRGLANVQQATLAAVA 179 RRAMRAAYQREMDALESHLAFLA+VGSVSPYVGLFGTVWGIMNAFRGLANVQQATLAAVA Sbjct: 121 RRAMRAAYQREMDALESHLAFLASVGSVSPYVGLFGTVWGIMNAFRGLANVQQATLAAVA 180 Query: 180 PGIAEALIATAIGLFAAIPAVIAYNRFSHDIDRLAIRFESFIEEFSNILQRQAR 233 PGIAEALIATAIGLFAAIPAV+AYNR+SHDIDRLAIRFESFIEEFSNILQRQAR Sbjct: 181 PGIAEALIATAIGLFAAIPAVVAYNRYSHDIDRLAIRFESFIEEFSNILQRQAR 234 >gi|300309940|ref|YP_003774032.1| biopolymer transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 231 Score = 91.7 bits (226), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 73/219 (33%), Positives = 101/219 (46%), Gaps = 23/219 (10%) Query: 5 QDLSFFALITNASVIVQFVMLLLLLASVFSWTYIFRKMFTIRSARSQTEEFERSFWSGGN 64 Q++ F + V LLL+ S+ SW YI K + R + ER FW Sbjct: 3 QNIGFAHYWAQGDAVSHSVAYLLLIMSIASWYYILSKAWAAWRIRRGSNALER-FWQAPT 61 Query: 65 LIALYQDTLSNRRKGGGHGGALERIFQAGMGEFNKAKAQVGK--------GATIDSSGLL 116 L T + +R E+I+ M E A +G A D L+ Sbjct: 62 LA---DATAAMQRLDS------EQIYTP-MAERAAEAADIGTRQQGTPSLNAATDPGELI 111 Query: 117 DGARRAMRAAYQREMDALESHLAFLATVGSVSPYVGLFGTVWGIMNAFRGLANVQQATLA 176 R +R R LE L LA+VGS +P+VGLFGTVWGI +A ++ + Sbjct: 112 T---RTLRREINRVSARLERGLTLLASVGSTAPFVGLFGTVWGIYHALVAVSASGTVQID 168 Query: 177 AVAPGIAEALIATAIGLFAAIPAVIAYNRFSHDIDRLAI 215 VA + EALI TA+GL AIPAV+AYN F+ I+R+ + Sbjct: 169 KVAGPVGEALIMTALGLLVAIPAVLAYNAFTR-INRVVL 206 >gi|300310025|ref|YP_003774117.1| biopolymer transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 243 Score = 90.1 bits (222), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 63/190 (33%), Positives = 88/190 (46%), Gaps = 18/190 (9%) Query: 19 IVQFVMLLLLLASVFSWTYIFRKMFTIRSARSQTEEFERSFWSGGNL----IALYQDTLS 74 + + V +LLL S+ SW I K + R + FW NL L QD Sbjct: 18 VTKGVAVLLLAMSIASWYVIITKALQLLRLRKASAAAGHQFWDTTNLPEGVATLGQDN-- 75 Query: 75 NRRKGGGHGGALERIFQAGMGEFNKAKAQVGKGATIDSSGLLDGARRAMRAAYQREMDAL 134 + +AG+ + A KG D + D ++R A L Sbjct: 76 ----------PFAEVAKAGVTAMSHHTAH--KGHLHDQLSVSDWVTLSLRQAIDEASGKL 123 Query: 135 ESHLAFLATVGSVSPYVGLFGTVWGIMNAFRGLANVQQATLAAVAPGIAEALIATAIGLF 194 ++ +A LA+VGS +P+VGLFGTVWGI +A + QA++ VA + EALI TA+GL Sbjct: 124 QAGMAVLASVGSTAPFVGLFGTVWGIYHALVSIGTTGQASIDKVAGPVGEALIMTALGLA 183 Query: 195 AAIPAVIAYN 204 AIPA YN Sbjct: 184 VAIPATFGYN 193 >gi|300312127|ref|YP_003776219.1| biopolymer transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 243 Score = 84.3 bits (207), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 64/212 (30%), Positives = 98/212 (46%), Gaps = 32/212 (15%) Query: 18 VIVQFVMLLLLLASVFSWTYIFRKMFTIRSARSQTEEFERSFWSG----GNLIALYQDTL 73 V+ +F++ +LL S SW + K R + + FW+ G + A+ Sbjct: 15 VVTRFLITILLGMSSVSWIVMIVKGMANRRLSRIGLKAHKEFWAAHSIEGGIAAI----- 69 Query: 74 SNRRKGGGHGGALERIFQAGMGEFNKAKAQVGKGATIDSSGLLDGARRAMRAAYQREMDA 133 G G I A + +A+AQ + S+ + +R ++ Sbjct: 70 -----GAGTDNPYSNIATAAV----EAQAQKDQPLLEGSTSNTEWVAYCVRNELDEQIAH 120 Query: 134 LESHLAFLATVGSVSPYVGLFGTVWGIMNAFRGLANVQQATLAAVAPGIAEALIATAIGL 193 L+ LA LA++GS SP++GLFGTVWGI +A + QA+L VA + E+L+ TA+GL Sbjct: 121 LQGGLAVLASIGSTSPFIGLFGTVWGIYHALIAIGASGQASLDHVAGPVGESLVMTAVGL 180 Query: 194 FAAIPAVIAYN--------------RFSHDID 211 F AIPAV+ YN RFSH + Sbjct: 181 FVAIPAVLGYNYVARGNRTVAQKLMRFSHQLH 212 >gi|300310500|ref|YP_003774592.1| biopolymer transport ExbB-like transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 201 Score = 55.1 bits (131), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 40/122 (32%), Positives = 59/122 (48%), Gaps = 5/122 (4%) Query: 116 LDGARRAMRAAYQREMDA----LESHLAFLATVGSVSPYVGLFGTVWGIMNAFRGLANVQ 171 +D R M+ A + A LE L L T+ S++P +GLFGTV G++ F N Sbjct: 81 VDAPREVMKEAIEEAGQAAAHRLERFLTTLGTIASLAPLMGLFGTVVGMIEIFAS-QNAA 139 Query: 172 QATLAAVAPGIAEALIATAIGLFAAIPAVIAYNRFSHDIDRLAIRFESFIEEFSNILQRQ 231 A +A GI+ AL T GL A+PA++ Y F +D + E +F +I+ Sbjct: 140 GTNPAQLAHGISIALYNTGFGLAIAMPALVFYRHFRALVDGFVVDMEQQAVKFVDIVHDV 199 Query: 232 AR 233 R Sbjct: 200 RR 201 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 681,031 Number of extensions: 29171 Number of successful extensions: 151 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 5 Length of query: 233 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 151 Effective length of database: 1,231,600 Effective search space: 185971600 Effective search space used: 185971600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 129 (54.3 bits)