BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0109.p (235 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogena... 417 e-118 gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 104 6e-24 gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogena... 89 4e-19 gi|300311727|ref|YP_003775819.1| dehydrogenase [Herbaspirillum s... 86 2e-18 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 79 2e-16 >gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 417 bits (1072), Expect = e-118, Method: Compositional matrix adjust. Identities = 199/235 (84%), Positives = 214/235 (91%) Query: 1 MARKFVQNGHKIIATGRRKDRLDALADEFGADKVLPLVLDVTDKHSIKSALASLPQGWQD 60 MARKF NGHK+IA GRRK+RLD L E GADKVLPLVLDVT K SI++ LASL W++ Sbjct: 16 MARKFAGNGHKVIAAGRRKERLDDLVKELGADKVLPLVLDVTSKSSIETGLASLTGAWKE 75 Query: 61 IDVLINNAGLALGLEPAHQVALEDWETMIDTNVKGLVTVTHHILPNMVKRGTGTIINLGS 120 +DVLINNAGLALGL PAH+VAL DWETMIDTNVKGLVT+TH +LP+MVKRG GTIINLGS Sbjct: 76 VDVLINNAGLALGLGPAHEVALSDWETMIDTNVKGLVTITHALLPDMVKRGRGTIINLGS 135 Query: 121 IAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLAPGLCGGTEFSNVRFKGND 180 +AGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLAPGLCGGTEFSNVRFKG+D Sbjct: 136 VAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLAPGLCGGTEFSNVRFKGDD 195 Query: 181 GAAAKVYEGTTPLTAVDIAEAAFWVATLPAHVNINYIELMPTCQGFGPLNIKRTQ 235 GAAAKVYEGTTPLTA DIAEAAFWVAT+PAHVNINY+ELMPTCQGF P NIKR Q Sbjct: 196 GAAAKVYEGTTPLTADDIAEAAFWVATMPAHVNINYMELMPTCQGFSPFNIKRKQ 250 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 104 bits (260), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 73/228 (32%), Positives = 115/228 (50%), Gaps = 6/228 (2%) Query: 2 ARKFVQNGHKIIATGRRKDRLDALADEFGA--DKVLPLVLDVTDKHSIKSALASLPQGWQ 59 AR G K++ RR +RL+ L + A + + + DV + +++ +A Q + Sbjct: 24 ARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIAVTTDVARRDDVEALVAKGEQHFG 83 Query: 60 DIDVLINNAGLALGLEPAHQVALEDWETMIDTNVKGLVTVTHHILPNMVKRGTGTIINLG 119 IDVL+NNAG+ + L P ++ +E+W+ MID NVKG++ LP + +G IIN+ Sbjct: 84 RIDVLVNNAGI-MPLAPMAKLKVEEWDRMIDVNVKGVLYGVAAALPRFAAQSSGHIINVS 142 Query: 120 SIAGAYPYPG-GNVYGATKAFVDQFTLNLRADLVGTGVRATNLAPGLCGGTEFSNVRFKG 178 S+AG + G G VY ATK V T LR + GVR T ++PG ++ K Sbjct: 143 SVAGIKVFAGMGTVYSATKFAVRALTEGLRTE-APDGVRTTIISPGAVDSELKTHSSDKE 201 Query: 179 NDGAAAKVYEGTTPLTAVDIAEAAFWVATLPAHVNINYIELMPTCQGF 226 A YE + + +A A + PA+V+I+ + L P Q F Sbjct: 202 TSEALLAWYEANQ-IPSDSVARAIAYAIEQPANVDISEVVLRPVAQEF 248 >gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 89.0 bits (219), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 79/227 (34%), Positives = 112/227 (49%), Gaps = 33/227 (14%) Query: 1 MARKFVQNGHKIIATGR--RKDRLDALADEFGADKVLPLVLDVTDKHSIKSALASLPQGW 58 +AR F+Q K+IAT R R D L A ++LPL DV D HSI++A+ + Sbjct: 17 IARYFLQRDWKVIATMRTPRHDLLPASPHL----RILPL--DVADAHSIQAAV----EAA 66 Query: 59 QDIDVLINNAGL----ALGLEPAHQVALEDWETMIDTNVKGLVTVTHHILPNMVKRGTGT 114 +DVL+NNAG+ AL +P QV + +TN G + +T +P M RG G Sbjct: 67 GPVDVLVNNAGIGNLGALEAQPMAQV-----RELFETNTLGSIAMTQAFIPQMRARGQGL 121 Query: 115 IINLGSIAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLAPGLCGGTEFS-N 173 +IN+ S P VY A+KA ++ FT +L +L GV+ + PG T F+ N Sbjct: 122 VINVTSSVTLRALPLLAVYTASKAAINAFTESLALELAPLGVQVRLVLPGRSPQTRFAEN 181 Query: 174 VRFKGNDGA-------AAKVYE---GTTPLT-AVDIAEAAFWVATLP 209 R + AA+V+E T LT A D+AEA + A P Sbjct: 182 ARARMAASVPEPYAALAAQVFENFQAATQLTYAEDVAEAVWQAANDP 228 >gi|300311727|ref|YP_003775819.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 86.3 bits (212), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 68/206 (33%), Positives = 99/206 (48%), Gaps = 17/206 (8%) Query: 2 ARKFVQNGHKIIATGRRKDRLDALADEFGADKVLPLVLDVTDKHSIKSALASLPQGWQDI 61 AR F+ NG K+IAT R R D L+ +D + L LDV+ SIK AL + I Sbjct: 18 ARHFLANGWKVIAT-MRSPRFDLLSP---SDNLCILPLDVSQPDSIKQAL----EAAGPI 69 Query: 62 DVLINNAGLALGLEPAHQVALEDWETMIDTNVKGLVTVTHHILPNMVKRGTGTIINLGSI 121 DVL+NNAG+ L + + +TN G++ + ++P +RG G IIN+ S Sbjct: 70 DVLVNNAGIGL-FGAFEATPMSTVREIFETNTFGVMAMCQAVIPQFRERGDGVIINVTSS 128 Query: 122 AGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLAPGLCGGTEFS---NVRFKG 178 A +P Y A+K ++ FT +L +L GVR + PG T F+ R +G Sbjct: 129 ATLAAFPLVAAYTASKTAIEGFTASLAHELKAVGVRVKLVEPGYGPSTSFATNGQQRMQG 188 Query: 179 -----NDGAAAKVYEGTTPLTAVDIA 199 + A +V+ G LT V A Sbjct: 189 LITEPYESFAHEVFAGFGQLTVVTAA 214 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 79.3 bits (194), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 50/171 (29%), Positives = 87/171 (50%), Gaps = 6/171 (3%) Query: 1 MARKFVQNGHKIIA----TGRRKDRLDALADEFGADKVLPLVLDVTDKHSIKSALASLPQ 56 + + ++G+ ++A RKD+ A E G D + +V++ S K+A + Sbjct: 19 ICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFD-IHASEGNVSNWESTKAAFEKVRA 77 Query: 57 GWQDIDVLINNAGLALGLEPAHQVALEDWETMIDTNVKGLVTVTHHILPNMVKRGTGTII 116 ++DVL+NNAG+ + +++ +DW+ +IDTN+ L VT ++ M +RG G II Sbjct: 78 EIGEVDVLVNNAGITRDGQ-FRKMSKDDWDAVIDTNLNSLFNVTKQVIEGMCERGFGRII 136 Query: 117 NLGSIAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLAPGLCG 167 N+ S+ G G Y KA + FT+ L ++ GV ++PG G Sbjct: 137 NISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPGYIG 187 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 808,153 Number of extensions: 36571 Number of successful extensions: 202 Number of sequences better than 1.0e-08: 36 Number of HSP's gapped: 170 Number of HSP's successfully gapped: 36 Length of query: 235 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 153 Effective length of database: 1,231,600 Effective search space: 188434800 Effective search space used: 188434800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)