BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0081.p (458 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312979|ref|YP_003777071.1| UDP-glucose 6-dehydrogenase [He... 816 0.0 gi|300311320|ref|YP_003775412.1| UDP-glucose 6-dehydrogenase [He... 661 0.0 gi|300312048|ref|YP_003776140.1| UDP-N-acetyl-D-mannosaminuronat... 101 1e-22 gi|300313478|ref|YP_003777570.1| NDP-N-acetyl-D-mannosaminuronat... 84 2e-17 >gi|300312979|ref|YP_003777071.1| UDP-glucose 6-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 816 bits (2109), Expect = 0.0, Method: Compositional matrix adjust. Identities = 388/457 (84%), Positives = 420/457 (91%) Query: 1 MKITIIGTGYVGLVTGACLAELGNDVFCLDVDQSKIDILNNGGIPIHEPGLEDVVARNRA 60 MKITIIGTGYVGLVTGACLAELGNDVFCLDVDQ K+DILNNGGIPIHEPGLE+VV RNRA Sbjct: 1 MKITIIGTGYVGLVTGACLAELGNDVFCLDVDQRKVDILNNGGIPIHEPGLEEVVERNRA 60 Query: 61 AGRLTFSTDVAASVAHGDVQFIAVGTPPDEDGSADLQYVLAAARNIGKHMTGFKVIVDKS 120 AGRL FSTDVAASVAHGD+QFIAVGTPPDEDGSADLQYVLAAARNIG++M GFKV+VDKS Sbjct: 61 AGRLQFSTDVAASVAHGDIQFIAVGTPPDEDGSADLQYVLAAARNIGRYMEGFKVVVDKS 120 Query: 121 TVPVGTAGKVSAAVAQELQQRGNVEAKFSVVSNPEFLKEGAAVEDFMRPDRIVIGHDNTA 180 TVPVGTA +V AA+A EL QRG E FSVVSNPEFLKEGAAVEDFMRPDRIVIGHD + Sbjct: 121 TVPVGTADRVKAAIADELAQRGKTELSFSVVSNPEFLKEGAAVEDFMRPDRIVIGHDQSP 180 Query: 181 EGMQAYAAMKKLYAPFNRNHERTYWMDVRSAEFTKYAANAMLATRISFMNELANLADQVG 240 EG+ A MKKLYAPFNRNHERTYWMDVRSAEFTKYAANAMLATRISFMNELANLAD+VG Sbjct: 181 EGLHAQTLMKKLYAPFNRNHERTYWMDVRSAEFTKYAANAMLATRISFMNELANLADKVG 240 Query: 241 ADIEAVRHGIGSDPRIGHSFLYAGCGYGGSCFPKDVQALERTARAYGQELHILRAVEQVN 300 ADIE+VRHGIGSDPRIGHSFLYAGCGYGGSCFPKDVQALERTAR Y Q L ILRAVEQVN Sbjct: 241 ADIESVRHGIGSDPRIGHSFLYAGCGYGGSCFPKDVQALERTARDYDQSLLILRAVEQVN 300 Query: 301 NNQKHVLGNKIVARFGENLQGRHFAVWGLAFKPNTDDMREASSRVLLGELIARGATVAVF 360 + QKHVLG K+VARFGE+L G+HFA+WGLAFKPNTDDMREASSRVL+GEL+ RGATVAV+ Sbjct: 301 DLQKHVLGKKVVARFGEDLAGKHFALWGLAFKPNTDDMREASSRVLIGELVRRGATVAVY 360 Query: 361 DPVAMKEARRVLALDFNDDESILNRIRFSESPMDALQDADALAIVTEWKAFRSPDFDQLK 420 DPVAMKEA RVL LDF DD ++L RIRF SPMD LQDADALAIVTEWKAFRSPDF+++K Sbjct: 361 DPVAMKEASRVLQLDFADDAALLQRIRFGTSPMDTLQDADALAIVTEWKAFRSPDFERVK 420 Query: 421 SKLKAPVIFDGRNLFEPQVMADAGIEYHGIGRSILTK 457 + LK PVIFDGRNLF+PQ+MAD+GIEY+GIGRS+L + Sbjct: 421 ATLKNPVIFDGRNLFDPQLMADSGIEYYGIGRSLLAR 457 >gi|300311320|ref|YP_003775412.1| UDP-glucose 6-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 661 bits (1705), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/452 (70%), Positives = 371/452 (82%) Query: 1 MKITIIGTGYVGLVTGACLAELGNDVFCLDVDQSKIDILNNGGIPIHEPGLEDVVARNRA 60 M IT+IGTGYVGLVTGACLAELGN VFCLDVD++KI+ILN+GG+PI+EPGL++++ARNRA Sbjct: 1 MNITVIGTGYVGLVTGACLAELGNKVFCLDVDRNKIEILNSGGVPIYEPGLKEIIARNRA 60 Query: 61 AGRLTFSTDVAASVAHGDVQFIAVGTPPDEDGSADLQYVLAAARNIGKHMTGFKVIVDKS 120 +GRL FSTDVAASVAHG+VQFIAVGTPPDEDGSADLQYV+AAARNIG+HM ++VDKS Sbjct: 61 SGRLIFSTDVAASVAHGEVQFIAVGTPPDEDGSADLQYVVAAARNIGRHMERNCIVVDKS 120 Query: 121 TVPVGTAGKVSAAVAQELQQRGNVEAKFSVVSNPEFLKEGAAVEDFMRPDRIVIGHDNTA 180 TVPVGT KV AA+ EL+ RG E +VSNPEFLKEGAAVEDFMRPDRIVIG + A Sbjct: 121 TVPVGTGEKVKAAIRAELEARGRGELDAMIVSNPEFLKEGAAVEDFMRPDRIVIGVEEGA 180 Query: 181 EGMQAYAAMKKLYAPFNRNHERTYWMDVRSAEFTKYAANAMLATRISFMNELANLADQVG 240 G +A M++LYAP NRNHER +MDV+SAEFTKYAANAMLATRISFMNELANLAD++G Sbjct: 181 AGAEAREVMRELYAPLNRNHERILYMDVKSAEFTKYAANAMLATRISFMNELANLADRLG 240 Query: 241 ADIEAVRHGIGSDPRIGHSFLYAGCGYGGSCFPKDVQALERTARAYGQELHILRAVEQVN 300 DIE VR GIGSDPRIG SFLYAG GYGGSCFPKDVQAL RTA +GQEL IL +VE VN Sbjct: 241 VDIEHVRKGIGSDPRIGFSFLYAGTGYGGSCFPKDVQALARTASEHGQELEILGSVESVN 300 Query: 301 NNQKHVLGNKIVARFGENLQGRHFAVWGLAFKPNTDDMREASSRVLLGELIARGATVAVF 360 + QK VL KIVAR GE+L G+ FAVWGLAFKPNTDDMR A SRV++ EL+ RGA V V+ Sbjct: 301 DKQKQVLLKKIVARLGEDLSGKTFAVWGLAFKPNTDDMRAAPSRVIVRELLQRGAKVQVY 360 Query: 361 DPVAMKEARRVLALDFNDDESILNRIRFSESPMDALQDADALAIVTEWKAFRSPDFDQLK 420 DPVAM+EAR ALD D + ++ M+AL+ ADA+ IVTEWK+F+SPDF +K Sbjct: 361 DPVAMEEARHAFALDLADLPGKEADLHYAGGAMEALEGADAVVIVTEWKSFKSPDFAAVK 420 Query: 421 SKLKAPVIFDGRNLFEPQVMADAGIEYHGIGR 452 + LK P++FDGRNLFEP MA G+EYH IGR Sbjct: 421 AALKEPLVFDGRNLFEPVTMARLGVEYHAIGR 452 >gi|300312048|ref|YP_003776140.1| UDP-N-acetyl-D-mannosaminuronate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 101 bits (252), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 126/455 (27%), Positives = 190/455 (41%), Gaps = 51/455 (11%) Query: 3 ITIIGTGYVGLVTGACLAELGNDVFCLDVDQSKIDILNNGGIPIHEPGLEDVVARNRAAG 62 + ++G GYVGL E G + D S+ I N +P E A RAA Sbjct: 4 VAVVGLGYVGL---PLAVEFGKKRATVGFDLSRSKIENYRRFV--DPTGEVSSAELRAAS 58 Query: 63 RLTFSTDVAASVAHGDVQFIAVGTPPDEDGSADLQYVLAAARNIGKHMTGFKVIVDKSTV 122 +LT STD A +A D +AV TP DE + D + A++ +GKHM ++V +STV Sbjct: 59 QLTVSTD-PAILADADYIVVAVPTPVDEAHNPDFGPLAGASQTVGKHMKRGAIVVFESTV 117 Query: 123 PVGTAGKVSAAVAQE---LQQRGNVEAKFSVVSNPEFLKEGAAVEDFMRPDRIVIGHD-N 178 G +V + ++ L+ R + FS PE + G +IV G D + Sbjct: 118 YPGATEEVCVPLLEKYSGLKWRDDFHVGFS----PERINPGDKQHTLTNILKIVSGDDAD 173 Query: 179 TAEGM-QAYAAMKKLYAPFNRNHERTYWMDVRSAEFTKYAANAMLATRISFMNELANLAD 237 T E + Q Y ++ + A +R ++ AE K N I+ MNEL+ + D Sbjct: 174 TLERIAQLYESI--ITAGVHRASS------IKVAEAAKVIENTQRDLNIALMNELSIIFD 225 Query: 238 QVGADIEAVRHGIGSDPRIGHSFLYAGCGY-GGSCFPKDVQALERTARAYGQELHILRAV 296 ++G D V G+ +FL G GG C D L A G ++ A Sbjct: 226 KIGIDTLEVLQAAGTK----WNFLPFRPGLVGGHCIGVDPYYLTHKAEMLGYHPQVILAG 281 Query: 297 EQVNNNQKHVLGNKIVARF---GENLQGRHFAVWGLAFKPNTDDMREASSRVLLGELIAR 353 ++N+ + K V G ++G V GL FK N D+R + ++ EL + Sbjct: 282 RRINDGMAKFVAEKTVKSMIAAGFPIKGSKVNVLGLTFKENCPDLRNSKVVDIVTELQSY 341 Query: 354 GATVAVFDPVAM---KEARRVLALDFNDDESILNRIRFSESPMDALQDADALAIVTEWKA 410 G V V DPVA E +AL+ D L ADA+ + Sbjct: 342 GVEVHVHDPVAAGVDAEHEYGIALENWSD----------------LPRADAVIAAVSHRE 385 Query: 411 FRSPDFDQLKSKLKAPVIF-DGRNLFEPQVMADAG 444 + +L+SKLK F D ++ F+ + AG Sbjct: 386 LLARPLAELQSKLKDGACFIDVKSAFDQPALEQAG 420 >gi|300313478|ref|YP_003777570.1| NDP-N-acetyl-D-mannosaminuronate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 424 Score = 84.0 bits (206), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 103/384 (26%), Positives = 162/384 (42%), Gaps = 44/384 (11%) Query: 1 MKITIIGTGYVGLVTGACLAELGND--VFCLDVDQSKIDILNNGGIPIHEPGLEDVVARN 58 +K+ +IG GYVGL E G V D++Q +ID L +G ++ LE Sbjct: 6 IKLAVIGLGYVGLPLAV---EFGRKRPVIGFDINQRRIDELKSG----NDFTLETTPEEL 58 Query: 59 RAAGRLTFSTDVAASVAHGDVQFIAVGTPPDEDGSADLQYVLAAARNIGKHMTGFKVIVD 118 AA +L+F+T + + + V TP DE DL ++ A+ +GK + +++ Sbjct: 59 SAATKLSFTTSLE-DLRTCSCYIVTVPTPIDEYKRPDLPPLIKASETVGKVLKKGDIVIY 117 Query: 119 KSTVPVGTAGKVSAAVAQELQQRGNVEAKFSVVSNPEFLKEGAAVEDFMRPDRIVIGHDN 178 +STV G + V EL F +PE + G + R+ Sbjct: 118 ESTVYPGCTEEDCVPVL-ELHSGLKFNEDFFCGYSPERINPGD------KEHRVTTIKKV 170 Query: 179 TAEGMQAYAAMKKLYAPFNR-----NHERTYWMDVRSAEFTKYAANAMLATRISFMNELA 233 T+ A A + + A +N H+ +R AE K N I+ +NELA Sbjct: 171 TSGSTPAVADI--VDALYNEIIVAGTHKAE---SIRVAEAAKVIENTQRDLNIALINELA 225 Query: 234 NLADQVGADIEAVRHGIGSDPRIGHSFLYAGCGY-GGSCFPKDVQALERTARAYGQELHI 292 + +++G D EAV GS +FL G GG C D L A+A G I Sbjct: 226 LIFNKMGIDTEAVLKAAGSK----WNFLPFRPGLVGGHCIGVDPYYLTHKAQAIGYHPEI 281 Query: 293 LRAVEQVNNNQKHVLGNKIVARFGE-------NLQGRHFAVWGLAFKPNTDDMREASSRV 345 + A ++N++ +G +VA+ + ++G V G FK N D+R Sbjct: 282 ILAGRRLNDS----MGTYVVAQLVKAMTKKRIQVEGAKVLVMGFTFKENCPDLRNTRVVD 337 Query: 346 LLGELIARGATVAVFDP-VAMKEA 368 ++ EL V V+DP V +EA Sbjct: 338 IVAELKDYNCEVDVYDPWVTTEEA 361 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,427,011 Number of extensions: 60439 Number of successful extensions: 193 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 190 Number of HSP's successfully gapped: 4 Length of query: 458 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 370 Effective length of database: 1,203,190 Effective search space: 445180300 Effective search space used: 445180300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)