BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0069.p (420 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312971|ref|YP_003777063.1| acyl-CoA transferases/carnitine... 721 0.0 gi|300312108|ref|YP_003776200.1| acyl-CoA transferases/carnitine... 158 7e-40 gi|300310600|ref|YP_003774692.1| acyl-CoA transferases/carnitine... 75 8e-15 >gi|300312971|ref|YP_003777063.1| acyl-CoA transferases/carnitine dehydratase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 721 bits (1860), Expect = 0.0, Method: Compositional matrix adjust. Identities = 353/420 (84%), Positives = 378/420 (90%), Gaps = 5/420 (1%) Query: 1 MTTTTPSPGPLAGIKVLELGTLIAGPFCSRMLAEFXFFSFGADVIKVEAPGVGDPLRQWR 60 MTT T PGPLAGIKVLELGTLIAGPFCSRMLAEF GADVIKVEAPG GDPLRQWR Sbjct: 1 MTTGTSKPGPLAGIKVLELGTLIAGPFCSRMLAEF-----GADVIKVEAPGNGDPLRQWR 55 Query: 61 VLKDGTSLWWSVQARNKKSLTLNMKDPQAQDIARKLALEADIIIENYRPGVLEKWNLGYE 120 VLKDGTSLWWSVQARNKKS+TLNMKD +AQ+IARKLALEAD+IIENYRPGVLEKW LGYE Sbjct: 56 VLKDGTSLWWSVQARNKKSITLNMKDERAQEIARKLALEADVIIENYRPGVLEKWKLGYE 115 Query: 121 DLKKLNPAIIMVRLSGYGQTGPLKDLPGFGAIGESMGGIRYVSGHQDRPPVRIGISIGDS 180 DLK+LNPA IMVRLSGYGQTGPL+DLPGFGAIGESMGGIRYVSGH DRPPVRIGISIGDS Sbjct: 116 DLKQLNPATIMVRLSGYGQTGPLRDLPGFGAIGESMGGIRYVSGHPDRPPVRIGISIGDS 175 Query: 181 VAALHGVIGAMMALRHRDVTGGRWNGKDGELRVAGQGQMVDVALYESVFNLMESLVPEFD 240 +AALHGVIGAMMALRHRDVTGGRWNGK G VAGQGQMVDVALYE+VFN+MES+VPEFD Sbjct: 176 IAALHGVIGAMMALRHRDVTGGRWNGKQGADCVAGQGQMVDVALYEAVFNMMESMVPEFD 235 Query: 241 QAGVVRERTGGALPGIVPSNTYTTADGKNVVIAGNGDAIFKRLMLAIDRADMANDPQLER 300 AGVVRERTGGALPGIVPSNTYTT +G N+VIAGNGDAIFKRLM AI R DMANDP L R Sbjct: 236 FAGVVRERTGGALPGIVPSNTYTTGEGMNIVIAGNGDAIFKRLMSAIGREDMANDPGLAR 295 Query: 301 NDGRVPRTEEIDRAIQAWCSGKTIDEALAILQAADVPVGKIYSVRDMMSDPQFLARNMFE 360 NDGRVPRTEEID AIQ WCS +TI+ ALA LQAADVPVGKIYSV+DMM+D QFLAR+MFE Sbjct: 296 NDGRVPRTEEIDAAIQQWCSTQTIETALATLQAADVPVGKIYSVKDMMNDAQFLARDMFE 355 Query: 361 QHAFADGTPIKLPAVTPKLSETPGGTKWIGPELGQHNAEILRSLGYDDAQIAQLRQDKVI 420 QH FADGTP+KLP +TPK+SETPG TKW+GPELG H+ EIL+SLGYD+A I LR V+ Sbjct: 356 QHQFADGTPVKLPGITPKMSETPGQTKWLGPELGAHSDEILQSLGYDEAHIKMLRDQGVV 415 >gi|300312108|ref|YP_003776200.1| acyl-CoA transferases/carnitine dehydratase [Herbaspirillum seropedicae SmR1] Length = 399 Score = 158 bits (400), Expect = 7e-40, Method: Compositional matrix adjust. Identities = 125/420 (29%), Positives = 200/420 (47%), Gaps = 34/420 (8%) Query: 5 TPSPGPLAGIKVLELGTLIAGPFCSRMLAEFXFFSFGADVIKVEAPGVGDPLRQWRVLKD 64 +PS PL GI V+ L IA PFC+R LA+ GA VIK+E PGVGD R + + Sbjct: 2 SPSHRPLEGITVISLEHAIAAPFCTRQLADL-----GARVIKIERPGVGDFARGYDSRVN 56 Query: 65 GTSLWWSVQARNKKSLTLNMKDPQAQDIARKLALEADIIIENYRPGVLEKWNLGYEDLKK 124 G S + R+K+SLTLN+KD + + I +L +AD++++N PG L + L Sbjct: 57 GLSSHFVWTNRSKESLTLNLKDEEGRRIIDQLLQQADVLVQNLAPGAAAGLGLSAQALHA 116 Query: 125 LNPAIIMVRLSGYGQTGPLKDLPGFGAIGESMGGIRYVSGHQDRPPVRIGISIGDSVAAL 184 +P +I+ +SGYG+ GP +D + + +S G V+G D V+ G SI D A + Sbjct: 117 RHPRLIVCDISGYGEDGPYRDKKAYDLLIQSEAGFLSVTGTPD-DVVKAGCSIADIAAGM 175 Query: 185 HGVIGAMMALRHRDVTGGRWNGKDGELRVAGQGQMVDVALYESVFNLMESLVPEFDQAGV 244 + + AL K G G+G +D+++ ES+ M + Q Sbjct: 176 YAYSNILAALI-----------KRGREGREGRGSHIDLSMLESLAEWMSFPLYYAYQGAS 224 Query: 245 VRERTGGALPGIVPSNTYTTADGKNVVIAGNGDAIFKRLM-LAIDRADMANDPQLERNDG 303 R+G I P ++ DGK V++ + +K + A +A+D + N Sbjct: 225 APLRSGAEHATIYPYGPFSAGDGKTVMLGLQNEREWKVFCEQVLQDAALASDARFSSNAR 284 Query: 304 RVPRTEEIDRAIQAWCSGKTIDEALAILQAADVPVGKIYSVRDMMSDPQFLARNMFEQHA 363 R + + + I +G + ++ A L A + ++ +++++ PQ AR + + A Sbjct: 285 RHEHRDVLRQLILDVFAGLSAEQVAARLDEAQIANARVNTMQELWQHPQLAARQRWTEVA 344 Query: 364 FADGTPI-KLPAVTPKLSETPGGTKWIG------PELGQHNAEILRSLGYDDAQIAQLRQ 416 TP+ K+ A+ P PG P LGQH IL +LGY+ QIA LR+ Sbjct: 345 ----TPVGKVAALLP-----PGANDSYDYRMEAIPALGQHTESILSALGYEAGQIAALRE 395 >gi|300310600|ref|YP_003774692.1| acyl-CoA transferases/carnitine dehydratase [Herbaspirillum seropedicae SmR1] Length = 469 Score = 75.5 bits (184), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 50/150 (33%), Positives = 75/150 (50%), Gaps = 17/150 (11%) Query: 10 PLAGIKVLELGTLIAGPFCSRMLAEFXFFSFGADVIKVEAPGVGDPLRQWRVLKDGTSLW 69 PL GI+VL+L ++AGP +R LA GA+V++++ G +P V+ Sbjct: 207 PLRGIRVLDLTRILAGPVATRFLA-----GLGAEVLRIDPYGWEEPNTVPEVVL------ 255 Query: 70 WSVQARNKKSLTLNMKDPQAQDIARKLALEADIIIENYRPGVLEKWNLGYEDLKKLNPAI 129 K+ LN+KDP D R+L ADI++ YR L K L ++LNPA+ Sbjct: 256 ------GKRCARLNLKDPADLDTLRRLMGHADILVHGYRSDALAKLGLDAAQRRELNPAL 309 Query: 130 IMVRLSGYGQTGPLKDLPGFGAIGESMGGI 159 I V L YG +G + GF ++ + GI Sbjct: 310 IDVSLDAYGWSGAWQCRRGFDSLIQMSTGI 339 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.138 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,516,108 Number of extensions: 70567 Number of successful extensions: 205 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 201 Number of HSP's successfully gapped: 3 Length of query: 420 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 332 Effective length of database: 1,203,190 Effective search space: 399459080 Effective search space used: 399459080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)