BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0068.p (345 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312970|ref|YP_003777062.1| nitrate/sulfonate/bicarbonate A... 608 e-175 gi|300312686|ref|YP_003776778.1| nitrate/sulfonate/bicarbonate A... 214 1e-56 gi|300312687|ref|YP_003776779.1| nitrate/sulfonate/bicarbonate A... 199 2e-52 gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate A... 59 5e-10 gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate A... 57 2e-09 >gi|300312970|ref|YP_003777062.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 345 Score = 608 bits (1568), Expect = e-175, Method: Compositional matrix adjust. Identities = 298/345 (86%), Positives = 321/345 (93%) Query: 1 MKFNLKQFAIALALFLAGYLTFNTNLFAQALEKPKVSIAVGGKNLFYYLPLTVAEQLGYF 60 MK + KQ IA LFLAGY +FN + AQALEKPKVSIAVGGKNLFYYLPLT+AEQLGYF Sbjct: 1 MKLSWKQLLIAAGLFLAGYFSFNGSAGAQALEKPKVSIAVGGKNLFYYLPLTIAEQLGYF 60 Query: 61 KDEGLQVEISDFAGGAKALQAMVGGSADVVSGAYEHTINMQAKNQPIVAFVLQGRAPQIV 120 KDEGLQVEISDFAGGAKALQAMVGGSADVVSGAYEHTINMQAKNQPIVAFVLQGRAPQIV Sbjct: 61 KDEGLQVEISDFAGGAKALQAMVGGSADVVSGAYEHTINMQAKNQPIVAFVLQGRAPQIV 120 Query: 121 LAASNKTMPNYKSVADLKGKKIGVTAPGSSTSIMASFVLAKAGLKPTDVSFIGVGAAAGA 180 LA +NKTMPNYK+VADLKGKKIGVTAPGSSTS+MAS+VLAKAGLKP+DVSFIGVGAAAGA Sbjct: 121 LAVNNKTMPNYKTVADLKGKKIGVTAPGSSTSVMASYVLAKAGLKPSDVSFIGVGAAAGA 180 Query: 181 LSALRSGQIDAIANLDPVISMLEQKNEIKIIADTRTLKDTNDVFGGPMPAATLYAPEAFI 240 +SA+RSGQIDAIANLDPVISMLEQ EIK IADTRTLKDTNDVFGGPMPAATLYAP AFI Sbjct: 181 ISAVRSGQIDAIANLDPVISMLEQSKEIKTIADTRTLKDTNDVFGGPMPAATLYAPAAFI 240 Query: 241 KKYPNTTQALTNAMVRALKWIQQAGPSDIIKAVPESYLLGDRALYISAFLKVREAMSPDG 300 +KYPNTTQALTNAMVRALKWIQ+AGPSDIIKAVPESYLLGDR+LYI AF+KVR+A+SPDG Sbjct: 241 QKYPNTTQALTNAMVRALKWIQKAGPSDIIKAVPESYLLGDRSLYIDAFMKVRQAISPDG 300 Query: 301 IFPDAGPATALRTLQAFEPDLASKKIDLSRTYTNEFARKANAKHK 345 FP GP TALRTL+AFEP LA K+IDLS+T+TN F +KANAK+K Sbjct: 301 YFPPGGPDTALRTLKAFEPSLADKQIDLSKTFTNSFVQKANAKYK 345 >gi|300312686|ref|YP_003776778.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 337 Score = 214 bits (544), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 110/302 (36%), Positives = 170/302 (56%) Query: 35 KVSIAVGGKNLFYYLPLTVAEQLGYFKDEGLQVEISDFAGGAKALQAMVGGSADVVSGAY 94 K++I VGG N YLP +AEQLGYFK+EGL VE+ G A ++ G+ V G Y Sbjct: 31 KITIMVGGINKMVYLPAKLAEQLGYFKEEGLDVELQSQPAGVDAENELLAGAVQAVVGFY 90 Query: 95 EHTINMQAKNQPIVAFVLQGRAPQIVLAASNKTMPNYKSVADLKGKKIGVTAPGSSTSIM 154 +H+I++Q K + I A V+ G+ P V S K P+++S+AD KGK +GVT GSST+ + Sbjct: 91 DHSIDLQTKGKEITAIVILGQVPGEVELVSTKAAPHFQSMADAKGKTLGVTGLGSSTNFL 150 Query: 155 ASFVLAKAGLKPTDVSFIGVGAAAGALSALRSGQIDAIANLDPVISMLEQKNEIKIIADT 214 ++ ++ G+ S + VGA ++A++ G+IDA +P S L + + +++ D Sbjct: 151 TQYLASRHGITSKQFSVLPVGADNTFIAAMKQGRIDAGMTTEPTASQLLKTGDAQVLVDL 210 Query: 215 RTLKDTNDVFGGPMPAATLYAPEAFIKKYPNTTQALTNAMVRALKWIQQAGPSDIIKAVP 274 RT++ T GG PA+++Y +++ + Q L A V+ L++IQ I +P Sbjct: 211 RTVEGTVKALGGLYPASSVYVQRSWLNSHKEDAQKLARAFVKTLRFIQTHTAEQIADKMP 270 Query: 275 ESYLLGDRALYISAFLKVREAMSPDGIFPDAGPATALRTLQAFEPDLASKKIDLSRTYTN 334 + Y ++ LYI A S DG P GP T L L F P++ K +DLS+TYT+ Sbjct: 271 KDYYGNNKPLYIQALTNSLPMYSQDGKMPKGGPQTVLAVLSGFNPNVKGKHVDLSKTYTD 330 Query: 335 EF 336 EF Sbjct: 331 EF 332 >gi|300312687|ref|YP_003776779.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 353 Score = 199 bits (506), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 109/314 (34%), Positives = 168/314 (53%), Gaps = 12/314 (3%) Query: 29 QALEKPKVSIAVGGKNLFYYLPLTVAEQLGYFKDEGLQVEISDFAGGAKALQAMVGGSAD 88 QA K+ I VGG N YLP +AE LGYFK+EGL VE+ G A ++ G+ Sbjct: 41 QARAADKIIIMVGGINKLIYLPPKLAESLGYFKEEGLDVELQSQPAGVDAENELLAGAVQ 100 Query: 89 VVSGAYEHTINMQAKNQPIVAFVLQGRAPQIVLAASNKTM------PNYKSVADLKGKKI 142 V G Y+H+I++Q+K + + + Q++L M + +S+ADLKGK + Sbjct: 101 AVVGYYDHSIDLQSKGKEVTSIA------QLLLVPGGMEMVRADLADSVRSMADLKGKTL 154 Query: 143 GVTAPGSSTSIMASFVLAKAGLKPTDVSFIGVGAAAGALSALRSGQIDAIANLDPVISML 202 GVT GSS+S +A ++ +AGLK D S + VGA ++A++ ++D +P S+L Sbjct: 155 GVTGLGSSSSFIAQYLANRAGLKAGDYSILPVGAGNTLIAAMKQKRVDVAWTTEPTTSIL 214 Query: 203 EQKNEIKIIADTRTLKDTNDVFGGPMPAATLYAPEAFIKKYPNTTQALTNAMVRALKWIQ 262 K++ D ++ T GG PA++LY +++ + +Q L V+ L++IQ Sbjct: 215 LASGAAKVLVDMSSVDGTKAALGGLYPASSLYVHRSWMNSHKAESQKLARVFVKTLRYIQ 274 Query: 263 QAGPSDIIKAVPESYLLGDRALYISAFLKVREAMSPDGIFPDAGPATALRTLQAFEPDLA 322 DI +P+ Y G +A+Y+ A SPDG P GP L+ + F P++ Sbjct: 275 THSAEDIADKMPKDYYGGSKAIYVQALKASLPMYSPDGKMPAGGPEQVLKVMAGFNPNIK 334 Query: 323 SKKIDLSRTYTNEF 336 K IDLS+TYTNEF Sbjct: 335 GKNIDLSKTYTNEF 348 >gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 59.3 bits (142), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 64/241 (26%), Positives = 110/241 (45%), Gaps = 23/241 (9%) Query: 26 LFAQALEKPKVSIAVGGKNLFYYLPLTVAEQLGYFKD----EGLQVEISDFAGGAKALQA 81 L + A + PKV + +Y P V + G+ ++ +G +V+ G +AL+ Sbjct: 24 LLSSAAQPPKV---IRLDYAYYSPPSLVLRKFGWLEEDLKAQGTEVKWVLSQGSNRALEY 80 Query: 82 MVGGSADVVSGAYEHTINMQAKNQPIVAFVLQGRAPQIVLAASNKTMPNYKSVADLKGKK 141 + S D S A + +A P+ A + R P+ A K P KS+ +LKGKK Sbjct: 81 LNSDSVDFGSTAGLAALLARANGNPVKAVYIYSR-PEWTALAVGKESP-VKSINELKGKK 138 Query: 142 IGVTAPGSSTSIMASFVLAKAGLKPTDVSFIGVGAAAGALSALRSGQIDAIANLDPVISM 201 I T G+ + L + GLK +DV + + A G +AL G++ A A LDP ++ Sbjct: 139 IAATK-GTDPYLFLLRALHENGLKKSDVEIVHLQHADGR-AALEQGRVAAWAGLDPHLAA 196 Query: 202 LEQKNEIKIIADTRTLKDTN-DVFGGPMPAATLYAPEAFIKKYPNTTQALTNAMVRALKW 260 E + ++I ++ N + +G L ++F ++P+ + + A RA W Sbjct: 197 SELEAGSRLI-----YRNVNFNTYG------FLNVSDSFAARHPDQVKRVIAAYERARSW 245 Query: 261 I 261 I Sbjct: 246 I 246 >gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 57.4 bits (137), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 57/208 (27%), Positives = 102/208 (49%), Gaps = 27/208 (12%) Query: 64 GLQVEISDFAGGAKALQAM-VGG---SADVVSGAYEHTINMQAKNQPIVAFVLQGR---A 116 G ++ +FA G L+A+ VGG SADV + QA + A+V Q A Sbjct: 67 GAKISWHEFASGLPLLEALNVGGVDLSADVADTV---PVFAQAAGAKL-AYVAQESPSPA 122 Query: 117 PQIVLAASNKTMPNYKSVADLKGKKIGVT-APGSSTSIMASFVLAKAGLKPTDVSFIGVG 175 Q ++ ++ + + +DLKGKKI VT A G ++A+ L KAGLK +DV + Sbjct: 123 AQAIVVRADSVL---RKASDLKGKKIAVTKAAGVHYLLIAT--LEKAGLKFSDVDAAYLS 177 Query: 176 AAAGALSALRSGQIDAIANLDPVISMLEQKNEIKIIADTRTLKDTNDVFGGPMPAATLYA 235 A G +A G +DA DP ++ ++++++++I++D R + D + A Sbjct: 178 PADG-RAAFERGSVDAWVTWDPFLAGVQRQSQVRILSDGRGVADYQRYY---------LA 227 Query: 236 PEAFIKKYPNTTQALTNAMVRALKWIQQ 263 +F + P + + + + + W++Q Sbjct: 228 SASFAQARPEVLRVVFDELQKTGHWVKQ 255 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 965,305 Number of extensions: 37057 Number of successful extensions: 152 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 155 Number of HSP's successfully gapped: 7 Length of query: 345 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 259 Effective length of database: 1,212,660 Effective search space: 314078940 Effective search space used: 314078940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)