BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0061.p (526 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310432|ref|YP_003774524.1| glucose-6-phosphate isomerase [... 800 0.0 gi|300311812|ref|YP_003775904.1| glucose-6-phosphate isomerase [... 557 e-160 >gi|300310432|ref|YP_003774524.1| glucose-6-phosphate isomerase [Herbaspirillum seropedicae SmR1] Length = 554 Score = 800 bits (2067), Expect = 0.0, Method: Compositional matrix adjust. Identities = 391/522 (74%), Positives = 434/522 (83%), Gaps = 3/522 (0%) Query: 1 MRDLFAADPQRFDHMTVKAAGILFDYSKNRVDNTTLKLLFELARERGVERQRAAMFNGEK 60 MR+LFA P RF+ M V+AAG+L DYSKNR+++ TL LL LARERGVE R AMF GEK Sbjct: 27 MRELFAQQPDRFERMHVRAAGLLLDYSKNRIESETLALLMSLARERGVEALREAMFRGEK 86 Query: 61 INFTEHRAVLHTALRAPRGQAL--QVDGQHIGADVHAVLDRIHGFTERVREGQWLGLSGK 118 INFTEHRAVLHTALRAP QA QVDGQ + +VHAVLD I F+ERVR G W G +G+ Sbjct: 87 INFTEHRAVLHTALRAP-AQACDAQVDGQPVSQEVHAVLDHIRDFSERVRSGAWRGYTGR 145 Query: 119 PITDIVNIGIGGSHLGPQMACTALRTFAHPRLTMHFVSNVDGHDLHDALQRVDVETTLFI 178 ITDIVNIGIGGS LGPQM CTALR + H RLT HFVSNVDGHDL + L RVD ETTLFI Sbjct: 146 EITDIVNIGIGGSFLGPQMVCTALRNYRHARLTTHFVSNVDGHDLDEVLSRVDPETTLFI 205 Query: 179 VASKTFTTQETMMNAGSARDWFLQRAGVDDLAKHFVAVSTNTEAVRAFGIDTDNMFPFWD 238 VASKTFTTQETM+NA SAR WFL+ DLA+HFVAVSTN V +FGID DNMFPFWD Sbjct: 206 VASKTFTTQETMLNAKSARAWFLRHGQQADLARHFVAVSTNAAEVESFGIDPDNMFPFWD 265 Query: 239 WVGGRYSMWSSIGLSIALAIGFKNFSELLAGAHAMDKHFCEAPLKQNMPIIQALIGIWNR 298 WVGGRYS+WS+IGL IAL IGF++FS LAGAHAMD+HF +APL+ NMP+IQALIGIWNR Sbjct: 266 WVGGRYSVWSAIGLPIALLIGFEHFSAFLAGAHAMDRHFRDAPLEANMPVIQALIGIWNR 325 Query: 299 NCFNSESLSIAPYHHDLRHFPAYLQQLEMESNGKRITRDGSAVGVATCPFIWGDVGTNGQ 358 N FNSESLS+APYH LR+FPAYLQQL+MESNGKRITRDG+ VGVATCPFIWGDVGTNGQ Sbjct: 326 NFFNSESLSVAPYHQWLRYFPAYLQQLDMESNGKRITRDGTPVGVATCPFIWGDVGTNGQ 385 Query: 359 HAFFQLLHQGTDITPIDFIAVLHATHDLPGHHAVLLANCFAQSEAFMRGKQAEEVREDLK 418 HAFFQLLHQGT++TPIDFIAVL +H + H VLLANCFAQSEAFMRGKQA+EVR DL+ Sbjct: 386 HAFFQLLHQGTNVTPIDFIAVLKPSHGIDEHQDVLLANCFAQSEAFMRGKQADEVRADLE 445 Query: 419 KLGVDDDEINFLLPHKTFNGNRPSNTLLLDALTPATLGALIALYEHKTFVQGTIWGINSF 478 + GVD E+ LLPHKTF+GNRPSNTLLL L+P LGA+IALYEHKTFVQG IWGINSF Sbjct: 446 RQGVDAAELAALLPHKTFDGNRPSNTLLLPELSPHALGAMIALYEHKTFVQGAIWGINSF 505 Query: 479 DQWGVELGKVLAKTIQSELSGEAVPARHDCSTNALIALAKKA 520 DQWGVELGKVLAKTI EL E PA HD STNALIALA++A Sbjct: 506 DQWGVELGKVLAKTIHGELRSEPNPALHDASTNALIALARQA 547 >gi|300311812|ref|YP_003775904.1| glucose-6-phosphate isomerase [Herbaspirillum seropedicae SmR1] Length = 570 Score = 557 bits (1436), Expect = e-160, Method: Compositional matrix adjust. Identities = 276/524 (52%), Positives = 356/524 (67%), Gaps = 9/524 (1%) Query: 4 LFAADPQRFDHMTVKAAGILFDYSKNRVDNTTLKLLFELARERGVERQRAAMFNGEKINF 63 LFAAD +R + +T +A GI DYSKN V + T+ LL +LAR+ G+ + AMF G++IN Sbjct: 47 LFAADDKRGESLTAEAVGIYLDYSKNLVTSETMALLMQLARQCGLPDRIEAMFRGDRINL 106 Query: 64 TEHRAVLHTALRAPRGQALQVDGQHIGADVHAVLDRIHGFTERVREGQWLGLSGKPITDI 123 TE+R+VLHTALR P + L VDGQ + A+V VL R+ GF +VR GQWLG +G PI + Sbjct: 107 TENRSVLHTALRKPASERLVVDGQDVVAEVQQVLRRMEGFARQVRSGQWLGHTGLPIRHV 166 Query: 124 VNIGIGGSHLGPQMACTALRTFAHPRLTMHFVSNVDGHDLHDALQRVDVETTLFIVASKT 183 VNIGIGGS LGP MA ALR ++H LT+HFVS+VDG + D ++ + E TLFIV SKT Sbjct: 167 VNIGIGGSDLGPMMAYEALRPYSHDDLTLHFVSDVDGAAMLDTVRGLPAEQTLFIVCSKT 226 Query: 184 FTTQETMMNAGSARDWFLQRAGVDD-LAKHFVAVSTNTEAVRAFGIDTDNMFPFWDWVGG 242 FTT ET+ NA +AR W +Q+ G ++ +A+HFV V+TN E + FGID DNMF FWDWVGG Sbjct: 227 FTTLETITNATAARAWLVQQLGAEEAVARHFVGVTTNIEEAQRFGIDADNMFGFWDWVGG 286 Query: 243 RYSMWSSIGLSIALAIGFKNFSELLAGAHAMDKHFCEAPLKQNMPIIQALIGIWNRNCFN 302 RYSM S+IGLS +AIG ++F +L G HAMD+HF APL+ N+P+I ++ +W N F Sbjct: 287 RYSMTSAIGLSTMVAIGPEHFRAMLEGFHAMDEHFRSAPLESNLPVILGMLTVWYTNFFG 346 Query: 303 SESLSIAPYHHDLRHFPAYLQQLEMESNGKRITRDGSAVGVATCPFIWGDVGTNGQHAFF 362 + S ++ PY L+ PAYLQQL MESNGK IT +G V T P WGD G NGQH+F+ Sbjct: 347 AASQAVLPYSQHLKRLPAYLQQLTMESNGKHITVNGEPVDYQTSPVYWGDNGINGQHSFY 406 Query: 363 QLLHQGTDITPIDFIAVLHATHDLPGHHAVLLANCFAQSEAFMRGKQAEEVREDLKKLGV 422 Q+LHQGT + P DFI + L H +L+AN FAQSEA GK A++VR++ G Sbjct: 407 QMLHQGTQLVPCDFIGLCQPQQALGQQHELLMANMFAQSEALAFGKDADQVRQE----GT 462 Query: 423 DDDEINFLLPHKTFNGNRPSNTLLLDALTPATLGALIALYEHKTFVQGTIWGINSFDQWG 482 + L+PH+ F GNRPSN++LL LTP +LG L+ALYEH F QG +W I+SFDQWG Sbjct: 463 PEA----LVPHRVFEGNRPSNSMLLQQLTPYSLGTLVALYEHSVFTQGVVWNIDSFDQWG 518 Query: 483 VELGKVLAKTIQSELSGEAVPARHDCSTNALIALAKKALSDTKN 526 VELGK LAK EL P HD STNALI + + T++ Sbjct: 519 VELGKQLAKRTARELIDHQQPLHHDSSTNALIRRYRACSTATQS 562 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,638,829 Number of extensions: 68783 Number of successful extensions: 198 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 195 Number of HSP's successfully gapped: 2 Length of query: 526 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 436 Effective length of database: 1,193,720 Effective search space: 520461920 Effective search space used: 520461920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)