BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0054.p (505 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 301 6e-83 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 239 3e-64 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 231 1e-61 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 204 2e-53 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 166 4e-42 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 301 bits (772), Expect = 6e-83, Method: Compositional matrix adjust. Identities = 182/511 (35%), Positives = 276/511 (54%), Gaps = 58/511 (11%) Query: 6 RSKNITQGVSRSPNRSMYYALGYEKEDFDKPMIGIANGHSTITPCNSGLQKLADVAISAI 65 RS TQG + + R+++ A G + DF KP+I + N + P + L+ L + I Sbjct: 5 RSYTTTQGRNMAGARALWRATGMKDGDFTKPIIAVVNSFTQFVPGHVHLKDLGQLVAREI 64 Query: 66 KQAGANPQVFGTPTISDGMSMGTEGMKYSLISREVIADCIETAVNGQWMDGVVVIGGCDK 125 + AG + F T + D + G M YSL SR++IAD +E VN D +V I CDK Sbjct: 65 EAAGGVAKEFNTIAVDDSGTHGDARMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDK 124 Query: 126 NMPGGMIALARTNVPGIYVYGGTIKPG--------NWKGKDLTIVSAFEAVGEFSAGRMS 177 PG ++A R N+P ++V GG ++ G + K L +V A G+ + +S Sbjct: 125 ITPGMLMAAMRLNIPVVFVSGGPMEAGKVIKTVNTDKKVIKLDLVDAMIQAGDKN---IS 181 Query: 178 QEDFDGIERNACPSSGSCGGMYTANTMSSAFEALGMGLFYSSTMANPDDEKTGSAAESAR 237 D +ER+ACP+ GSC GM+TAN+M+ E LG+ L + T+ ++ + R Sbjct: 182 DADVAEVERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAGR 241 Query: 238 VLVEAVKK-------DIKPRDIITRKSIENAVSLIMATGGSTNAVLHFLAIAHAAEVEWT 290 ++VE K+ I PR+I T+++ ENA++L ++ GGSTN VLH LA AH A+VE+T Sbjct: 242 LIVELAKRHYEQDDYSILPRNICTKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEFT 301 Query: 291 IDDFERVRQKVPVICDLKP-SGKYVATDLHKAGGIPAVLKVLHEAGLLHGDCLTITGKTL 349 + D +R+ ++VP +C + P + KY D+H+AGGI ++L L AGLL T+ KTL Sbjct: 302 MADIDRISRRVPCLCKVAPMTNKYHIEDVHRAGGIISILGELARAGLLDTSRPTVHSKTL 361 Query: 350 AEELANIPGLRADQD------------------------------------VIRPIDKAL 373 E + +R D IR + A Sbjct: 362 GEAIEKFD-IRVSSDPAVHKLFRAAPGGVPTQVAFSQEERFEANDLDRANGCIRDREHAY 420 Query: 374 YKQGHLAILKGNLAPEGCVAKITGLKNPVI--TGPARVFDDEYSAMDAILADKIKPGDVL 431 + G LA+L GN+A +GC+ K G+ ++ +G ARVF+ + +A++ IL DK+K GDV+ Sbjct: 421 SQDGGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGDKVKAGDVV 480 Query: 432 VMRYLGPKGGPGMPEMLAPTSALIGKGLGES 462 ++RY GPKGGPGM EML PTS + KGLG++ Sbjct: 481 IVRYEGPKGGPGMQEMLYPTSYIKSKGLGKA 511 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 239 bits (611), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 155/435 (35%), Positives = 245/435 (56%), Gaps = 19/435 (4%) Query: 20 RSMYYALGYEKEDFD-KPMIGIANGHSTITPCNSGLQKLADVAISAIKQAGANPQVFGTP 78 RS G + FD +P+IGI N +S +TPCNS + LA+ I +AG P F Sbjct: 27 RSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNSHFRALAEQVKIGIWEAGGFPLEFPV- 85 Query: 79 TISDGMSMGTEGMK-YSLISREVIADCIETAVNGQWMDGVVVIGGCDKNMPGGMIALART 137 MS+G ++ +++ R + + +E ++ +DGVV++ GCDK P ++ A Sbjct: 86 -----MSLGETLLRPTAMLYRNLASMDVEESIRANPLDGVVLLMGCDKTTPALLMGAASV 140 Query: 138 NVPGIYVYGGTIKPGNWKGKDL-TIVSAFEAVGEFSAGRMSQEDFDGIERNACPSSGSCG 196 +VP I V GG + G ++G++L + ++ + AG+M+QE+F E S G C Sbjct: 141 DVPTIGVSGGPMLSGKYRGRELGSGTGVWQMSEDVRAGKMTQEEFFEAESCMHRSHGHCM 200 Query: 197 GMYTANTMSSAFEALGMGLFYSSTMANPDDEKTGSAAESARVLVEAVKKDIKPRDIITRK 256 M TA+TM+S EALG+GL +++ + D + A + R +V+ V++D+ I+TR+ Sbjct: 201 TMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAGRRIVQMVEEDLVLSKILTRQ 260 Query: 257 SIENAVSLIMATGGSTNAVLHFLAIAHAAEVEWTIDDFERVRQKVPVICDLKPSGKYVAT 316 + ENA+ + A GGSTNAV+H LAIA ++ + D++ + Q++P + DL+PSGK++ Sbjct: 261 AFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDLKDWDDIGQQLPCLLDLQPSGKFLME 320 Query: 317 DLHKAGGIPAVLKVLHEAGLLHGDCLTITGKTLAEELANIPGLRADQDVIRPIDKALYKQ 376 D + AGG+PAV++ L ++ LT GKTL E + P +++VIR DK + Sbjct: 321 DFYYAGGLPAVIRQLES--VIDKTALTANGKTLWENCQDAPNW--NEEVIRSFDKPFKEA 376 Query: 377 GHLAILKGNLAPEGCVAKITGLKNPVIT--GPARVF---DDEYSAMDAILADKIKPGDVL 431 +AILKGNLAP+G V K + ++ G A VF DD + +D D + VL Sbjct: 377 AGIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHKRIDDENLD-VDETCVL 435 Query: 432 VMRYLGPKGGPGMPE 446 V++ GPKG PGM E Sbjct: 436 VLKNCGPKGYPGMAE 450 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 231 bits (589), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 154/438 (35%), Positives = 244/438 (55%), Gaps = 20/438 (4%) Query: 20 RSMYYALGYEKEDFD-KPMIGIANGHSTITPCNSGLQKLADVAISAIKQAGANPQVFGTP 78 RS G +F KP+IGI N S +TPCN+ +K+A+ I +AG P F P Sbjct: 29 RSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNAHFRKIAEHVRRGIIEAGGFPVEF--P 86 Query: 79 TISDGMSMGTEGMKYSLISREVIADCIETAVNGQWMDGVVVIGGCDKNMPGGMIALARTN 138 S+G S ++++R + + +E ++ G +D VV++ GCDK P ++ A + Sbjct: 87 VFSNGES---NLRPTAMLTRNLASMDVEESIRGNPIDAVVLLTGCDKTTPALLMGAASCD 143 Query: 139 VPGIYVYGGTIKPGNWKGKDL---TIVSAFEAVGEFSAGRMSQEDFDGIERNACPSSGSC 195 VP I V GG + G +G+D+ T+V ++ + AG ++ DF E S+G+C Sbjct: 144 VPAIVVTGGPMLNGKHQGRDIGSGTVV--WQLSEQVKAGEITIHDFMAAEAGMSRSAGTC 201 Query: 196 GGMYTANTMSSAFEALGMGLFYSSTMANPDDEKTGSAAESARVLVEAVKKDIKPRDIITR 255 M TA+TM+ E+LG+ L +++ + D + A S +V+ V + + I+TR Sbjct: 202 NTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHLSGMRIVDMVWEGLTLSKILTR 261 Query: 256 KSIENAVSLIMATGGSTNAVLHFLAIAHAAEVEWTIDDFERVRQKVPVICDLKPSGKYVA 315 K+ ENA+ A GGSTNAV+H AIA V+ ++D+ R+ + P I DL+PSG+Y+ Sbjct: 262 KAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDWTRIGRGTPTIVDLQPSGRYLM 321 Query: 316 TDLHKAGGIPAVLKVLHEAGLL-HGDCLTITGKTLAEELANIPGLRADQDVIRPIDKALY 374 + + AGG+PAVL+ L EA LL H D LT+ G+T+ + + + P + +V+RP+ K L Sbjct: 322 EEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWDNVKDAPIY--NDEVVRPLAKPLI 379 Query: 375 KQGHLAILKGNLAPEGCVAKITGLKNPVIT--GPARVFDDEYSAMDAILAD---KIKPGD 429 + G + IL+GNLAP G V K + ++ G A VF+D ++ + D + Sbjct: 380 EDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFED-FNHYKERINDPDLDVDASC 438 Query: 430 VLVMRYLGPKGGPGMPEM 447 VLVM+ +GPKG PGM E+ Sbjct: 439 VLVMKNVGPKGYPGMAEV 456 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 204 bits (518), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 153/460 (33%), Positives = 234/460 (50%), Gaps = 30/460 (6%) Query: 20 RSMYYALGYEKEDFDKPMIGIANGHSTITPCNSGLQKLADVAISAIKQAGANPQVFGTPT 79 R M Y + E+ +P+IGIA S I+PCN +LA I+ AG P F Sbjct: 32 RFMNYGITAEELRSGRPIIGIAQSGSDISPCNRIHLELAKRVRDGIRDAGGIPMEFPLHP 91 Query: 80 ISDGMSMGTEGMKYSLISREVIADCIETAVNGQWMDGVVVIGGCDKNMPGGMIALARTNV 139 I + T + I R + + ++G +D VV+ GCDK P ++A A ++ Sbjct: 92 IFENCRRPT-----AAIDRNLAYLGLVEILHGYPIDAVVLTTGCDKTTPSQIMAAATVDI 146 Query: 140 PGIYVYGGTIKPGNWKGKDLTIVSA-FEAVGEFSAGRMSQEDFDGIERNACPSSGSCGGM 198 P I + GG + G G+ + SA ++ SAG + E F I + PSSG C M Sbjct: 147 PAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLSAGSIDNEKFLEIAAASAPSSGHCNTM 206 Query: 199 YTANTMSSAFEALGMGLFYSSTMANPDDEKTGSAAESARVLVEAVKKDIKPRDIITRKSI 258 TA+TM++ EALGM L S + P E+ A E+ R +V +D++P I+TR + Sbjct: 207 GTASTMNAMAEALGMSLTGCSAIPAPYRERGQMAYETGRRIVGMAYEDLRPSAILTRDAF 266 Query: 259 ENAVSLIMATGGSTNAVLHFLAIAHAAEVEWTIDDFERVRQKVPVICDLKPSGKYVATDL 318 +A+ + A GGSTNA H +A+A A VE +D+ + VP++ +++P+GKY+ Sbjct: 267 LDAIVVNAAIGGSTNAQPHIMAMARHAGVELQSEDWMKYGYDVPLLLNMQPAGKYLGERF 326 Query: 319 HKAGGIPAVLKVLHEAGLLHGDCLTITGKTLAEELANIPGLRA--DQDVIRPIDKALYKQ 376 H+AGG+PA++ L +AG L + +T TGKT+AE L RA D+++I P L ++ Sbjct: 327 HRAGGVPAIMWELQQAGKLRAERITATGKTMAENLQG----RASNDREMIYPFAAPLRER 382 Query: 377 GHLAILKGNLAPEGCVAKIT----GLKNPVITGP---------ARVFD--DEYSAMDAIL 421 +LKGNL + + K + + ++ P A VFD D+Y A Sbjct: 383 AGFLVLKGNLF-DFAIMKTSVISETFRERYLSTPGQENIFECRAVVFDGSDDYHARINDP 441 Query: 422 ADKIKPGDVLVMRYLGPKGGPGMPEM--LAPTSALIGKGL 459 A KI +L +R GP G PG E+ + P ALI +G+ Sbjct: 442 ALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRGV 481 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 166 bits (420), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 134/452 (29%), Positives = 216/452 (47%), Gaps = 38/452 (8%) Query: 30 KEDFDKPMIGIANGHSTITPCNSGLQKLADVAISAIKQAGANPQVFG-TPTISDGMSMGT 88 KED P + I + ++ + + ++ + A ++AGA Q G +P + DG++ G Sbjct: 63 KED-KAPSLAIVSAYNDMLSAHQPFERFPQIIKDAAREAGAVAQFAGGSPAMCDGVTQGQ 121 Query: 89 EGMKYSLISREVIADCIETAVNGQWMDGVVVIGGCDKNMPGGMI-ALARTNVPGIYVYGG 147 GM+ SL SR+ IA A++ D + +G CDK +PG +I AL ++P ++V G Sbjct: 122 AGMELSLFSRDAIAMATAIALSHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVPAG 181 Query: 148 TIKPGNWKGKDLTIVSAFEAVGEFSAGRMSQEDFDGIERNACPSSGSCGGMYTANTMSSA 207 + G + I + +AG++ + + E + +G+C TAN+ Sbjct: 182 PMTSGMSNSDKVKIRQLY------TAGKIGRAELLEAESKSYHGAGTCTFYGTANSNQML 235 Query: 208 FEALGMGL----FYSSTMANPDDEKTGSAAESARVLVEAVKKDIKPRDIITRKSIENAVS 263 EA+G+ L F + D +A ++A++ + R +I KSI NA+ Sbjct: 236 MEAMGLHLPGAAFITPNTPLRDALTAAAAKQAAKITDLGTQYTPVGR-MIDEKSIVNAIV 294 Query: 264 LIMATGGSTNAVLHFLAIAHAAEVEWTIDDFERVRQKVPVICDLKPSGKYVATDLHKAGG 323 + ATGGSTN LH +AIA AA + DDF+++ VP+I + P+G H AGG Sbjct: 295 ALHATGGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYPNGTADVNHFHAAGG 354 Query: 324 IPAVLKVLHEAGLLHGDCLTITGKTLAEELA-------------------NIPGLRADQD 364 VL+ L +AGL+H D TI G+ L + + P D+ Sbjct: 355 TGFVLRELIDAGLMHDDVTTILGQGLRQHCKEPMLAAEGENGRPGIVTWHDAPAQSGDEG 414 Query: 365 VIRPIDKALYKQGHLAILKGNLAPEGCVAKITGLK--NPVITGPARVFDDEYSAMDAILA 422 V+ + K G L +L+GNL V KI+ +K + V+ PA VF + + M A A Sbjct: 415 VLGTVAKPFAADGGLKLLQGNLGR--AVIKISAVKPEHRVVEAPAVVFHSQEAFMAAFKA 472 Query: 423 DKIKPGDVLVMRYLGPKGGPGMPEMLAPTSAL 454 ++ V V+ + GP+ GMPE+ A T AL Sbjct: 473 GQLDRDFVAVITFQGPRAN-GMPELHALTPAL 503 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.134 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,637,863 Number of extensions: 71305 Number of successful extensions: 226 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 206 Number of HSP's successfully gapped: 6 Length of query: 505 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 416 Effective length of database: 1,198,455 Effective search space: 498557280 Effective search space used: 498557280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)