BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0051.p (380 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC t... 695 0.0 gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 351 5e-98 gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC t... 250 2e-67 gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 184 9e-48 gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC t... 133 3e-32 >gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 380 Score = 695 bits (1793), Expect = 0.0, Method: Compositional matrix adjust. Identities = 344/380 (90%), Positives = 360/380 (94%) Query: 1 MKLKSTIIPLTGAIALAFAGAAYSQEVIKIAHVGPLSGPNAHMGKDNENGARMAIDELNA 60 MKLKS IIPLT AI LAFAGAAYSQEV+KIAHVGPLSGPNAHMGKDNENGARMA+DELNA Sbjct: 1 MKLKSAIIPLTAAIGLAFAGAAYSQEVVKIAHVGPLSGPNAHMGKDNENGARMAVDELNA 60 Query: 61 KGFMIGGKKVKFELIGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYNDA 120 KGF IGGKKVKFEL+GEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIY+DA Sbjct: 61 KGFTIGGKKVKFELVGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYSDA 120 Query: 121 GIPQVSPSATNPKYTQQGFKTAFRVVANDAQLGAVLGKYAVTALKTKNVAVIDDRTAYGQ 180 GIPQVSPSATNPKYTQQGFKTAFRVVANDAQLGA LGKYAV L TK +AVIDDRTAYGQ Sbjct: 121 GIPQVSPSATNPKYTQQGFKTAFRVVANDAQLGAALGKYAVQKLGTKQIAVIDDRTAYGQ 180 Query: 181 GVAEEFAKGAQGAGATIVGKQFTNDKATDFNAILTSIKAKKPEVVFFGGMDAVGGPMLRQ 240 GVA+EFAKGA+ AG TIVG Q+TNDKATDFNAILTS+K KKP+VVFFGGMDAVGGPMLRQ Sbjct: 181 GVADEFAKGAKAAGGTIVGTQYTNDKATDFNAILTSLKGKKPDVVFFGGMDAVGGPMLRQ 240 Query: 241 MKQLGITAKFMGGDGICTGSLPGLAGDGLIDDQVVCAEAGGVDESGKKGMDDFRAAYKKK 300 MKQLGI+AKFMGGDGICTGSLPGLAGDGL DDQV+CAEAGGVDE+GKKGMDDFRAAYKKK Sbjct: 241 MKQLGISAKFMGGDGICTGSLPGLAGDGLGDDQVICAEAGGVDEAGKKGMDDFRAAYKKK 300 Query: 301 FGIDVVYNAAYAYDATLTVADAMKKANSADPAKYLPELAKISHKGVTGTIAFDAKGDIKN 360 FGIDVVYNAAYAYDAT+TVADAM KA SADP KYLPELAKISHKGVTG IAFDAKGDIK+ Sbjct: 301 FGIDVVYNAAYAYDATMTVADAMAKAGSADPKKYLPELAKISHKGVTGVIAFDAKGDIKD 360 Query: 361 GSLTLYTYKKGERTLLAVVK 380 GSLTLYTYK G+RTLLAV K Sbjct: 361 GSLTLYTYKGGQRTLLAVTK 380 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 351 bits (901), Expect = 5e-98, Method: Compositional matrix adjust. Identities = 192/366 (52%), Positives = 240/366 (65%), Gaps = 4/366 (1%) Query: 6 TIIPLTGAIALAFAGAAYSQEVIKIAHVGPLSGPNAHMGKDNENGARMAIDELNAKGFMI 65 II + A+ AFA A +Q V+KI PL+GP A GKDN+ G MAI+ LNA+ + Sbjct: 9 NIIAASLALIPAFAMAQETQ-VVKIGFSSPLTGPQASAGKDNQGGLMMAIERLNAQPITV 67 Query: 66 GGKKVKFELIGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYNDAGIPQV 125 GGKK+KF++I EDD +DPK AVA KL DQ V A++G NSG TIPAS++YNDAGI V Sbjct: 68 GGKKIKFDVIAEDDQADPKSGVAVAQKLADQGVKAIVGPYNSGVTIPASRVYNDAGI-VV 126 Query: 126 SPSATNPKYTQQGFKTAFRVVANDAQLGAVLGKYAVTALKTKNVAVIDDRTAYGQGVAEE 185 + A+NPK TQQGF T FRV A+D+QLG + YA LK K VAVIDDRTAYGQG+A+E Sbjct: 127 ATVASNPKITQQGFATLFRVAASDSQLGGKMALYAAKELKFKRVAVIDDRTAYGQGLAQE 186 Query: 186 FAKGAQGAGATIVGKQFTNDKATDFNAILTSIKAKKPEVVFFGGMDAVGGPMLRQMKQLG 245 F K A+ G +V FTNDKATDF AILTSIK KKP+ VF GG GGP+ RQMKQLG Sbjct: 187 FIKVAKANGIDVVSTDFTNDKATDFTAILTSIKGKKPDAVFLGGYAPQGGPIKRQMKQLG 246 Query: 246 ITAKFMGGDGICTGSLPGLAGDGLIDDQVVCAEAGGVDESGKKGMDDFRAAYKKKFGIDV 305 + MGGDGIC+ + L GD I + V C + G + + K+G F Y+KK+ Sbjct: 247 VDVPLMGGDGICSPEMGRLGGDA-IGESVYCTQGGTMLDKAKEG-KVFSDEYQKKYNRPA 304 Query: 306 VYNAAYAYDATLTVADAMKKANSADPAKYLPELAKISHKGVTGTIAFDAKGDIKNGSLTL 365 A YD + +A AMK+ANS DP ++ P LAKIS+KGV G FDA D+K +T+ Sbjct: 305 ETYAVSFYDGMMLIAQAMKQANSVDPKQFGPALAKISYKGVAGQYDFDANHDLKQSPVTV 364 Query: 366 YTYKKG 371 Y +K G Sbjct: 365 YRFKDG 370 >gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 394 Score = 250 bits (638), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 127/342 (37%), Positives = 199/342 (58%), Gaps = 3/342 (0%) Query: 28 IKIAHVGPLSGPNAHMGKDNENGARMAIDELNAKGFMIGGKKVKFELIGEDDASDPKQAT 87 + I GPLSGP+ +GK +NG ++A+ E N +G IGGK + LI +DD +DP A Sbjct: 40 LAIGFAGPLSGPSGGVGKSMQNGVQLALAEANRRGLRIGGKSWQLRLIAQDDRADPATAE 99 Query: 88 AVATKLVDQKVAAVIGHLNSGTTIPASKIYNDAGIPQVSPSATNPKYTQQGFKTAFRVVA 147 VA LV Q+V AV+GH SGT++ A+ +Y+ AG+ QV+PSA + + T + FR + Sbjct: 100 FVARSLVQQQVIAVVGHWTSGTSLAAAPVYHRAGVIQVTPSAMSRRLTAMSYPRIFRTIP 159 Query: 148 NDAQLGAVLGKYAVTALKTKNVAVIDDRTAYGQGVAEEFAKGAQGAGATIVGKQFTNDKA 207 N+ LG + YAV L+T+ + IDDRT +GQG+AE+FA+ A GA +VG+ +DK Sbjct: 160 NNESLGRLAAGYAVDKLETRTIVTIDDRTPFGQGLAEQFARTASERGAQVVGRYSVSDKT 219 Query: 208 TDFNAILTSIKAKKPEVVFFGGMDAVGGPMLRQMKQLGITAKFMGGDGICTGSLPGLAGD 267 +DFNA L + +P+++FFGG+D G + + +++L + A+ M G T LP L Sbjct: 220 SDFNAALLEARKLQPDLIFFGGLDWQAGVLAKSIRRLKLHARLMASPG--TLGLPFLMRA 277 Query: 268 GLIDDQVVCAEAGGVDESGKKGMDDFRAAYKKKFGIDVVYNAAYAYDATLTVADAMKKAN 327 G + + E G + G FR Y + F D+ A +AY+A + +++A Sbjct: 278 GPDANGALVLEPGPPQDK-MPGWKSFRQRYSENFDSDMDLYAVFAYEAAQAIIQGIRQAG 336 Query: 328 SADPAKYLPELAKISHKGVTGTIAFDAKGDIKNGSLTLYTYK 369 SADP + + ++ +GV+G +AF+ +GD+ S T+Y K Sbjct: 337 SADPERIAEAMHRLRFEGVSGPVAFNEEGDLLQPSFTMYEVK 378 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 184 bits (467), Expect = 9e-48, Method: Compositional matrix adjust. Identities = 123/349 (35%), Positives = 184/349 (52%), Gaps = 14/349 (4%) Query: 26 EVIKIAHVGPLSGPNAHMGKDNENGARMAIDELNAKGFMIGGKKVKFELIGEDDASDPKQ 85 E +KIA GP++G A G + GA AI+++NA G G KFE + DDA +PKQ Sbjct: 27 ETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQINAAG---GANGNKFEAVMMDDACEPKQ 83 Query: 86 ATAVATKLVDQKVAAVIGHLNSGTTIPASKIYNDAGIPQVSPSATNPKYTQ-QGFKTAFR 144 A AVA K+V Q + VIGH+ SG+TIPAS IY + G+ ++PSAT P+ T+ + K FR Sbjct: 84 AVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTEAKPHKFIFR 143 Query: 145 VVANDAQLGAVLGKYAVTALKTKNVAVIDDRTAYGQGVAEEFAKGAQGAGATIVGKQFTN 204 + D Q G +Y + LK K VAV+ D+ +YGQGVA A +V + N Sbjct: 144 TIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQGVASSVKAALDAAKVPVVVFEGIN 203 Query: 205 DKATDFNAILTSIKAKKPEVVFFGGMDAVGGPMLRQMKQLGITAKFMGGDGICTGSLPGL 264 +D++AI+T +K++ + V+FGG G ++RQ ++ GI A FMG +G+ + + Sbjct: 204 AGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGIKAVFMGPEGVGNKDITAI 263 Query: 265 AGDGLIDDQVVCAEAGGVDESGKKGMDDFRAAYKKKFGIDVVYNAAY---AYDATLTVAD 321 AG + +V A + + AA K+ N + AY +AD Sbjct: 264 AGPAS-EGMLVTLPADFAADPANAALVKAFAAAKRD------PNGPFQMPAYSGVKIIAD 316 Query: 322 AMKKANSADPAKYLPELAKISHKGVTGTIAFDAKGDIKNGSLTLYTYKK 370 A+ A S DP K + S K G + +D KGD+K+ ++T+ K Sbjct: 317 AIAGAKSTDPEKVAAYIHANSFKTPIGNVEYDKKGDLKSFKFVVFTWHK 365 >gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 133 bits (334), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 105/373 (28%), Positives = 180/373 (48%), Gaps = 30/373 (8%) Query: 10 LTGAIAL-AFAGAAYSQE--VIKIAHVGPLSGPNAHMGKDNENGARMAIDELNAKGFMIG 66 L G + L AF A ++QE I + G LS P + + GA++AIDE NA + Sbjct: 10 LAGVLLLWAFMQAGWAQERKTIALGFTGRLSEP---IAQSALQGAQLAIDEANA----LA 62 Query: 67 GKK---VKFELIGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYNDAGIP 123 K+ ++FEL+ +DD + A +A V +KVA VIG +S + + IY A IP Sbjct: 63 AKRRSPLRFELLPQDDHGNENFAVNIARYYVKEKVAGVIGPWSSDAALATAAIYESAQIP 122 Query: 124 QVSPSATNPKYTQQGFKTAFRVVANDAQLGAVLGKYAVTALKTKNVAVIDDRTAYGQGVA 183 Q+ +AT+ ++T G + FRVV +L A L + A +L+ + + VI AY + +A Sbjct: 123 QIGFTATSSQWTGMGNRMPFRVVGGTRELAATLAEVATRSLQGRRIFVIQSDAAYSRALA 182 Query: 184 EEFAKGAQGAGATIVGKQF-TNDKATDFNAILTSIKAKKPEVVFFGGMDAVGGPMLRQMK 242 + + G+ I ++ ++TDF+A L + +A + +V+ F G+ + Sbjct: 183 DTLVENLGGSAQQIALARYQVGRRSTDFHAALKAAEAHQADVIVFVGLYPQARAFIDGAN 242 Query: 243 QLGITAKFMGGDGICTGSLPGLAGDGL--IDDQVV---CAEAGGVDESGKKGMDDFRAAY 297 + TA+ + G SLP L ++ ++ C + V ++ Y Sbjct: 243 RTRTTARLLLTGGASNLSLPAPLELRLHVLEYEIARPHCPQWKVVTQT-----------Y 291 Query: 298 KKKFGIDVVYNAAYAYDATLTVADAMKKANSADPAKYLPELAKISHKGVTGTIAFDAKGD 357 +++FG + YAYDA + A+ +A S + K L ++ H G+ GT++FDA G Sbjct: 292 QRRFGAAPTTYSFYAYDAASVLTAAIVQAGSDNGDKIATALHRMRHAGLHGTLSFDATGA 351 Query: 358 IKNGSLTLYTYKK 370 + TL Y++ Sbjct: 352 NAHPRYTLLRYQE 364 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.133 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,144,852 Number of extensions: 50183 Number of successful extensions: 176 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 168 Number of HSP's successfully gapped: 15 Length of query: 380 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 293 Effective length of database: 1,207,925 Effective search space: 353922025 Effective search space used: 353922025 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)