BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0049.p (465 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, m... 573 e-164 gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux trans... 171 1e-43 gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium... 136 4e-33 gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium... 82 6e-17 >gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, membrane component protein [Herbaspirillum seropedicae SmR1] Length = 587 Score = 573 bits (1477), Expect = e-164, Method: Compositional matrix adjust. Identities = 284/328 (86%), Positives = 309/328 (94%) Query: 1 MHAVELFIQDLAVIMLIAGVVTVVFNRFKQPVVLGYIVAGVIIGPHTPPFNLIQDDKTVH 60 MH VELFIQD+A+IMLIAG+VTVVFN+ KQPVVLGYIVAGVIIGPHTPP++LIQD+KTVH Sbjct: 1 MHEVELFIQDMAIIMLIAGIVTVVFNKLKQPVVLGYIVAGVIIGPHTPPYDLIQDEKTVH 60 Query: 61 ILSELGVIFLLFSLGLEFSLKKLAKVGATAVVAAVAEIMLMIWIGYEIGIYFGWKTMDAV 120 ILSELGVIFLLFSLGLEFSLKKLAKVGATA + A AEI+LMIWIGYEIG+YFGWK MDA+ Sbjct: 61 ILSELGVIFLLFSLGLEFSLKKLAKVGATAFIGAAAEILLMIWIGYEIGLYFGWKRMDAI 120 Query: 121 FLGAMLAVSSTTIIVKALNELGMKNEKFAQIIFGILIVEDILAIGMIALLSGIATSGSVD 180 FLGAMLAVSSTTIIVKALNELGMKNEKFAQIIFGILIVEDILAIGMIALLSG+ATSGSVD Sbjct: 121 FLGAMLAVSSTTIIVKALNELGMKNEKFAQIIFGILIVEDILAIGMIALLSGVATSGSVD 180 Query: 181 SGEVVNTVGKLLLFMVVSLVVGILIVPRLLNYVARFKSNEMLLVTVLGILFGFCLLVMKL 240 S EV TVGKL+LFM VSLVVGIL VPRLL++++RFKSNEMLLV VLG+LFGFCLLVMKL Sbjct: 181 STEVFTTVGKLVLFMTVSLVVGILAVPRLLSFISRFKSNEMLLVAVLGVLFGFCLLVMKL 240 Query: 241 EYSVALGAFLVGAVMAESRQIHRIERIIESIRDMFSAIFFVAIGLLFDPNVLAKYWLPIT 300 +YSVALGAFLVGAVMAESR +HRIER++E IRDMFSAIFFVAIGLLFDPNVL KYW+PIT Sbjct: 241 QYSVALGAFLVGAVMAESRHLHRIERLVEPIRDMFSAIFFVAIGLLFDPNVLVKYWMPIT 300 Query: 301 VITFAVVFGKLFSCGIGTFLAGHGGRXP 328 VIT AVVFGKL SCG+GTF+AG GGR P Sbjct: 301 VITLAVVFGKLISCGLGTFIAGQGGRTP 328 >gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux transporter NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 171 bits (434), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 115/306 (37%), Positives = 170/306 (55%), Gaps = 12/306 (3%) Query: 15 MLIAGVVTVVFNRFKQPVVLGYIVAGVIIGPHTPPFNLIQDDKTVHILSELGVIFLLFSL 74 +++A + + +R K P ++GY+VAG+ IGP TP F + D L+E+GVI L+F + Sbjct: 15 LVLAFIFGTLAHRLKMPPLVGYLVAGIAIGPFTPGF--VGDADLAQELAEIGVILLMFGV 72 Query: 75 GLEFSLKKLAKVGATAVVAAVAEIMLMIWIGYEIGIYFGWKTMDAVFLGAMLAVSSTTII 134 GL FSLK L V A+ AVA+I + +G +G GW + G L+V+ST ++ Sbjct: 73 GLHFSLKDLLSVKNIAIPGAVAQITIATLLGMALGWLLGWPVGEGFLFGLALSVASTVVL 132 Query: 135 VKALNELGMKNEKFAQIIFGILIVEDI---LAIGMIALLSGI-ATSGSVDSGEVV----- 185 +KAL E + + +I G LIVEDI LA+ +I LSGI G SG V Sbjct: 133 LKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEALSGNAVLLTLG 192 Query: 186 NTVGKLLLFMVVSLVVGILIVPRLLNYVARFKSNEMLLVTVLGILFGFCL-LVMKLEYSV 244 T+GK++ F V L+VG ++P +L +A S E+ + VL I GF L S Sbjct: 193 ITLGKVVAFAAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFALGSAYVFGVSF 252 Query: 245 ALGAFLVGAVMAESRQIHRIERIIESIRDMFSAIFFVAIGLLFDPNVLAKYWLPITVITF 304 ALGAF G +++ES HR +RD FS +FFV++G+LFDP+++A+ L + Sbjct: 253 ALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIVAREPLLVLATVL 312 Query: 305 AVVFGK 310 +V GK Sbjct: 313 IIVVGK 318 >gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium-efflux system K+/H+ antiporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 663 Score = 136 bits (342), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 96/300 (32%), Positives = 162/300 (54%), Gaps = 19/300 (6%) Query: 10 DLAVIMLIAGVVTVVFNRFKQ-PVVLGYIVAGVIIGPHTPPFNLIQDDKTVHILSELGVI 68 +L + +L A V+ VV R Q P +LGY+V G+IIGPH F +D++T H L E GV+ Sbjct: 6 ELTLFLLAAAVLGVVAFRMMQLPPMLGYLVVGIIIGPHGLGFA--EDNETTHALGEFGVV 63 Query: 69 FLLFSLGLEFSLKKLAK-------VGATAVVAAVAEIMLMIW-IGYEIGIYFGWKTMDAV 120 FL+FS+GLEFSL KL+ +G V +A M++ W + +F A Sbjct: 64 FLMFSIGLEFSLAKLSAMRHIVFGLGVAQVATTIAATMVLGWGAATFLPQFFNISWQAAF 123 Query: 121 FLGAMLAVSSTTIIVKALNELGMKNEKFAQIIFGILIVEDILAIGMIALLSGIATSGSVD 180 LG L +SST I+ K L E + + I G+L+ +D+ + ++ ++ +A + Sbjct: 124 ALGGALTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVPALAN----E 179 Query: 181 SGEVVNTVG----KLLLFMVVSLVVGILIVPRLLNYVARFKSNEMLLVTVLGILFGFCLL 236 SG ++ T+G K L+ + + L G ++ V + +S E+ ++ +L I G + Sbjct: 180 SGNIMVTLGWAAGKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWI 239 Query: 237 VMKLEYSVALGAFLVGAVMAESRQIHRIERIIESIRDMFSAIFFVAIGLLFDPNVLAKYW 296 K S+ALGAF+ G +++E+ H++E I+S RD+ +FF+ IG+L + + +W Sbjct: 240 TEKAGLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTVIDHW 299 >gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium-efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 606 Score = 82.4 bits (202), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 83/309 (26%), Positives = 144/309 (46%), Gaps = 46/309 (14%) Query: 11 LAVIMLIAGVVTV-VFNRFKQPVVLGYIVAGVIIGPHTPPFNLIQDDKTVHILSELGVIF 69 +A++ L+A V V + R VLGY++AG++IGP LI + K + SE GV+ Sbjct: 7 IALVYLLAAVAAVPIAKRLGLGAVLGYLLAGIVIGPWG--LGLITEVKHILEFSEFGVVL 64 Query: 70 LLFSLGLEFSLKKLAKVGATAVVAAVAEIMLMIWIGYEIGIYFGWKTMDAV--------- 120 LLF +GLE K+L + + FGW ++ Sbjct: 65 LLFLIGLELDPKRLWALRRS---------------------IFGWGSIQVAAVSLALLAA 103 Query: 121 -----------FLGAM-LAVSSTTIIVKALNELGMKNEKFAQIIFGILIVEDILAIGMIA 168 +GA+ +++SST I + L E + F IL+ +DI AI M+A Sbjct: 104 ALAASVPWRIGLIGALGMSLSSTAIALATLGERKLTATPAGSAAFSILLFQDIAAIPMMA 163 Query: 169 LLSGIATSGSVDSGEVVNTVGKLLLFMVVSLVVGILIVPRLLNYVARFKSNEMLLVTVLG 228 + + + S SG+ G++ L + + + G +++ +L +A+ E+ L Sbjct: 164 AVPLLGVAASQGSGQGWLHAGQVALVIALLIFGGRVLMRPILRIIAKTDMREIFTAFALL 223 Query: 229 ILFGFCLLVMKLEYSVALGAFLVGAVMAESRQIHRIERIIESIRDMFSAIFFVAIGLLFD 288 ++ CLL+ + S+ALG FL G ++A+S H +E +E + + +FF+A+G+ D Sbjct: 224 LVIATCLLMQSVGMSMALGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVD 283 Query: 289 PNV-LAKYW 296 V A+ W Sbjct: 284 FGVFFAQPW 292 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.145 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,098,147 Number of extensions: 38697 Number of successful extensions: 196 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 188 Number of HSP's successfully gapped: 4 Length of query: 465 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 376 Effective length of database: 1,198,455 Effective search space: 450619080 Effective search space used: 450619080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)