BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0048.p (140 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, m... 235 1e-63 >gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, membrane component protein [Herbaspirillum seropedicae SmR1] Length = 587 Score = 235 bits (599), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 116/140 (82%), Positives = 130/140 (92%) Query: 1 VDGIGGRLSTWIGDEQVRKAVIWGGALVLSLPFLIATYRKLQALAMLLAEVGVKPEFAGA 60 VDG+ +LS W D V+KA+IWG ALVLSLPFLIATYRKLQAL+MLLAEV VKPEFAGA Sbjct: 448 VDGLSRQLSGWTPDPDVQKAIIWGCALVLSLPFLIATYRKLQALSMLLAEVSVKPEFAGA 507 Query: 61 YTSGVRRVISEVIPVMSILGIMLLIFVLSASILPPLNLLAFVLLGAAGLLWLLWSKLVKL 120 YTSGVR+++SEV+P+MSI+GIMLLIFVLSASILPPLNLLAFVL GAAGLLWLLWSKLVKL Sbjct: 508 YTSGVRKIVSEVLPIMSIVGIMLLIFVLSASILPPLNLLAFVLAGAAGLLWLLWSKLVKL 567 Query: 121 HSRLQIALFETLDEKPEDSH 140 HSRLQIALFETLDE+P+D+H Sbjct: 568 HSRLQIALFETLDEQPDDAH 587 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.142 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 333,183 Number of extensions: 10487 Number of successful extensions: 59 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 59 Number of HSP's successfully gapped: 1 Length of query: 140 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 64 Effective length of database: 1,260,010 Effective search space: 80640640 Effective search space used: 80640640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 126 (53.1 bits)