BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0042.p (611 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbasp... 773 0.0 gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum s... 119 9e-28 >gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbaspirillum seropedicae SmR1] Length = 929 Score = 773 bits (1997), Expect = 0.0, Method: Compositional matrix adjust. Identities = 375/627 (59%), Positives = 469/627 (74%), Gaps = 50/627 (7%) Query: 20 LPAGVSQGPSAEGITEYRFSNGFKLLLMPDASKPTVTVNMTYLVGSRQENYGETGMAHLL 79 LPAGVS GPSAEGI+EYRF+NGFKLLL+PD S+PTVTVN+TYLVGSR ENYGETGMAHLL Sbjct: 47 LPAGVSAGPSAEGISEYRFANGFKLLLLPDDSQPTVTVNITYLVGSRHENYGETGMAHLL 106 Query: 80 EHLMFKGTPQHGAIPQEFSKRGMSFNGSTSLDRTNYYEFFPAGDENLQWAIVMEADRMLN 139 EHLMFKG+P+H +IPQ+FSKRGM+FNG+T LDRTNYYE F A +NL+WAI MEADRMLN Sbjct: 107 EHLMFKGSPRHPSIPQDFSKRGMNFNGTTWLDRTNYYETFQASPDNLRWAIAMEADRMLN 166 Query: 140 SFIAQKDLDSEMTVVRNEFESGENSPGSVMVKRMQSIAFDWHNYGNSTIGNRSDIEHVKI 199 S IA+KDLDSEMTVVRNEFE+GE SP VM+KRMQS+A+DWH YG +TIG RSDIE+V+I Sbjct: 167 SKIARKDLDSEMTVVRNEFEAGETSPTRVMLKRMQSVAYDWHAYGRNTIGARSDIENVRI 226 Query: 200 ENLQAFYRTYYQPDNAVLLVAGKFDPAKVLAWVDRSFGKMPKPARAV------------- 246 ENLQAFYRTYYQPDNAVLL+AGKFD A+VL WVD+SFG++PKP R + Sbjct: 227 ENLQAFYRTYYQPDNAVLLIAGKFDAAQVLQWVDQSFGRLPKPKRTLPAFWTVEPTQDGE 286 Query: 247 --------------------------------VAADILADTPNGRLHKLLVETGKASAIM 274 AADIL DTPNGRLHK LVETG A+ + Sbjct: 287 RQFVIRRRGDQQLVALAYKMPSALHPDATALGFAADILTDTPNGRLHKALVETGMATEVY 346 Query: 275 QFQLSGYAPGLQIIGAVIKKGEPLEPVRKALTDAIESFATTPPTDAEMARVRVSNANGYE 334 L G APGLQ++ A +K G L+ VR+A+ A+ESF+T PPT E+AR+ AN +E Sbjct: 347 AMPLEGMAPGLQMMVAKVKVGGDLDAVRQAMISAVESFSTQPPTPEEVARLHREAANSFE 406 Query: 335 RLLSDHQRIGISLSNVLALGDWRLLFAGRDQVDAMSAEQVAGAAAKYFRRDNRTVGLFIP 394 L ++ Q++ +++SN +A GDWRLLF RD++ +S+ +A AA +YFRRDNRTVGL++P Sbjct: 407 TLQNNPQQLAVAMSNAIARGDWRLLFIERDRMQRLSSADIAAAAGRYFRRDNRTVGLYLP 466 Query: 395 EDQPQRSEIPVAPSLESALQSYRPRAAIATGEVFDTSPANLDLRTTQLRVG-----GLKL 449 +D PQR+EIP AP+L++ L Y+PR AIA+GE FD +PAN++ RTT++ G L L Sbjct: 467 DDHPQRAEIPTAPALDTLLSQYQPRTAIASGEAFDPAPANIEQRTTRIAPGQPPHTALDL 526 Query: 450 ALLPKKTRGQTVSVRIKLDWGDEKTLFGKKTIAAMTDEMLSRGSMSMTREQLADEFARLK 509 ALLPK++RGQTVSV + L +GD ++LFG++ +A +T+ ML RG+ + R QLADEFARLK Sbjct: 527 ALLPKQSRGQTVSVSLHLRFGDAQSLFGQRNVAQLTEAMLMRGTDKLDRRQLADEFARLK 586 Query: 510 IAGGVYNFQTTRGNLDAALKLMADVLQHPRFEPAEFEQLRKQLLVSLESSRNDPTERASE 569 I G +Y FQTTR NL A+ L+ +V +HPR + AEFEQL+ + + LE+ R DP RA E Sbjct: 587 INGSLYRFQTTRENLVPAIALVGEVFRHPRMDAAEFEQLKNETVAGLEAGRKDPDTRAGE 646 Query: 570 ALSLHFDHYPPGDWRAARTLDQRIAAV 596 ALS+HFDHYP GDWRAA+TL+QRIAAV Sbjct: 647 ALSVHFDHYPAGDWRAAQTLEQRIAAV 673 Score = 60.1 bits (144), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 72/278 (25%), Positives = 122/278 (43%), Gaps = 40/278 (14%) Query: 11 LAQTPPALPLPAGVSQGPSAEGITE--YRFSNG------FKLLLMPDASK-PTVTVNMTY 61 L+Q P + +G + P+ I + R + G L L+P S+ TV+V++ Sbjct: 485 LSQYQPRTAIASGEAFDPAPANIEQRTTRIAPGQPPHTALDLALLPKQSRGQTVSVSLHL 544 Query: 62 LVGSRQENYGETGMAHLLEHLMFKGTPQ--HGAIPQEFSKRGMSFNGSTSLDRTNYYEFF 119 G Q +G+ +A L E ++ +GT + + EF++ + NGS +T Sbjct: 545 RFGDAQSLFGQRNVAQLTEAMLMRGTDKLDRRQLADEFAR--LKINGSLYRFQTTRENLV 602 Query: 120 PAGDENLQWAIVMEADRMLNSFIAQKDLDS-EMTVVRNE----FESGENSPGSVMVKRMQ 174 PA A+V E R +D+ E ++NE E+G P + + + Sbjct: 603 PA------IALVGEVFR-------HPRMDAAEFEQLKNETVAGLEAGRKDPDTRAGEAL- 648 Query: 175 SIAFDWHNYGN----STIGNR-SDIEHVKIENLQAFYRTYYQPDNAVLLVAGKFDPAKVL 229 S+ FD + G+ T+ R + + V +E ++ F+R +Y L V G FDPA+ Sbjct: 649 SVHFDHYPAGDWRAAQTLEQRIAAVRAVTLEQVKDFHRRFYGASQGELAVVGDFDPAQAR 708 Query: 230 AWVDRSFGKMPKPARAVVAADILADTPNGRLHKLLVET 267 + +D++FG A AD P R LLV+T Sbjct: 709 SAIDQAFGGWTSAAPYARVLQTWADIPAKR---LLVDT 743 >gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum seropedicae SmR1] Length = 459 Score = 119 bits (297), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 68/214 (31%), Positives = 114/214 (53%), Gaps = 5/214 (2%) Query: 30 AEGITEYRFSNGFKLLLMPDASKPTVTVNMTYLVGSRQENYGETGMAHLLEHLMFKGTPQ 89 AE ++ NG K+++ D PT + Y VG E G TG++H LEH+MFKGT Sbjct: 26 AEPARQFVLGNGMKVIVKEDRRAPTAVQMVWYKVGGIDEVNGLTGVSHALEHMMFKGTRN 85 Query: 90 H--GAIPQEFSKRGMSFNGSTSLDRTNYYEFFPAGDENLQWAIVMEADRMLNSFIAQKDL 147 H G + ++ G N T+ D T Y++ +L+ + +EADRM N + Sbjct: 86 HKVGEFSRLVAELGGQENAFTANDFTAYFQQIE--KSHLEKVMALEADRMANLQFDAAEF 143 Query: 148 DSEMTVVRNEFE-SGENSPGSVMVKRMQSIAFDWHNYGNSTIGNRSDIEHVKIENLQAFY 206 E+ V+ E ++ P ++ + + + A+ H Y + +G D++H+ ++++ A+Y Sbjct: 144 AKEIRVIMEERRWRTDDQPMGLLNEALNAAAWTAHPYHHPVVGWMDDLQHMSVQDIAAWY 203 Query: 207 RTYYQPDNAVLLVAGKFDPAKVLAWVDRSFGKMP 240 R +Y P+NA L+VAG D +VLA + FGK+P Sbjct: 204 RQWYAPNNATLVVAGDVDAQRVLALARKYFGKIP 237 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,852,589 Number of extensions: 77469 Number of successful extensions: 248 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 245 Number of HSP's successfully gapped: 4 Length of query: 611 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 520 Effective length of database: 1,188,985 Effective search space: 618272200 Effective search space used: 618272200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)