BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0036.p (307 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312750|ref|YP_003776842.1| LysR family transcription regul... 432 e-122 gi|300309393|ref|YP_003773485.1| LysR family transcription regul... 208 3e-55 gi|300312961|ref|YP_003777053.1| transcription regulator protein... 155 5e-39 gi|300313102|ref|YP_003777194.1| LysR family transcription regul... 148 7e-37 gi|300309864|ref|YP_003773956.1| LysR family transcription regul... 143 2e-35 >gi|300312750|ref|YP_003776842.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 322 Score = 432 bits (1111), Expect = e-122, Method: Compositional matrix adjust. Identities = 215/305 (70%), Positives = 251/305 (82%) Query: 3 TIDLRLLRYFIAVAEEGHLTKAAQRIGIQQPPLSQQIRALEKELGVTLFNRLPRGMELTE 62 +IDLRLLRYFIAVAEEGHLTKAA+RIGIQQPPLSQQIR LE+ELGVTLFNRLPRGMELT+ Sbjct: 18 SIDLRLLRYFIAVAEEGHLTKAAERIGIQQPPLSQQIRVLERELGVTLFNRLPRGMELTD 77 Query: 63 SGQALLTDARQILAQVDSTVAGVRRIARGELGRIAVGFTESASLHPFVPTVIRAFRDAVP 122 SG ALL DAR +LAQ+D+TVA VRRIA+G++GRIAVGFTESASLHPFV +VIRAFR P Sbjct: 78 SGAALLIDARALLAQLDTTVASVRRIAQGKMGRIAVGFTESASLHPFVHSVIRAFRADAP 137 Query: 123 DVALTVEESSTTDLVALMSQNRIDVAFIRSPVGDVSDITIETMMVEEMIVALPAAHPLVR 182 DVA+TVEES+T +L+ + R+D+AF+RSPVGD +TIETM+ E+M+ ALP HPLV Sbjct: 138 DVAMTVEESNTNELIEALRGKRLDLAFVRSPVGDSGGLTIETMLEEDMVAALPIDHPLVD 197 Query: 183 NKRRKSVALAALAEEVFILNRRPSGPGLYDTIIAACRAAGFSPRVEQEARKNLSTLSLVA 242 +RKS+AL ALAEE FI+ RRP+GPGLYDTIIAAC AAGFSP+V QEARKNLSTLSLVA Sbjct: 198 GGKRKSIALHALAEESFIMTRRPNGPGLYDTIIAACHAAGFSPQVIQEARKNLSTLSLVA 257 Query: 243 AGLGISIIPASMRHVELESVSYLGIDDAPDLKAPLHLAYRDSGQSGAVKRFIAEARKCAK 302 AG+G+++IPASM V E V YL + APDLKAPLHLAYR SGA + EA K + Sbjct: 258 AGVGVTVIPASMTRVNAEGVVYLKLTGAPDLKAPLHLAYRTGSISGAARLMREEALKLSA 317 Query: 303 AAGKR 307 +R Sbjct: 318 GRKRR 322 >gi|300309393|ref|YP_003773485.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 296 Score = 208 bits (530), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 126/302 (41%), Positives = 190/302 (62%), Gaps = 6/302 (1%) Query: 4 IDLRLLRYFIAVAEEGHLTKAAQRIGIQQPPLSQQIRALEKELGVTLFNRLPRGMELTES 63 ++LR LRYF+AVAE+ T+AA+++GI QPPLS QI+ALE+E+G LF R +G+EL+E Sbjct: 1 MELRHLRYFLAVAEQRSFTRAAEKVGIGQPPLSMQIKALEEEVGGALFLRSSQGVELSEV 60 Query: 64 GQALLTDARQILAQVDSTVAGVRRIARGELGRIAVGFTESASLHPFVPTVIRAFRDAVPD 123 G+ LL A++ + Q + + R+ A GE G + +GFT SA HP V ++ FR+ Sbjct: 61 GRVLLPHAQRAVDQAEQALRAARQAASGETGLLRMGFTSSAVFHPLVSHALQQFRERHRK 120 Query: 124 VALTVEESSTTDLVALMSQNRIDVAFIRSPVGDVSDITIETMMVEEMIVALPAAHPLVRN 183 + +++ E T L+A ++ +++D AF+R + + + + E++ VALP +HPL Sbjct: 121 LEISLSEGITEQLLADIAASQLDAAFVRIVTTPSAALGVVELPAEKLKVALPRSHPLA-- 178 Query: 184 KRRKSVALAALAEEVFILNRRPSGPGLYDTIIAACRAAGFSPRVEQEARKNLSTLSLVAA 243 RRKS+ L LA E FI+ R G LYD+I +AC+AAGF P+V Q A + S ++LVA+ Sbjct: 179 -RRKSLRLQDLASENFIMVPRGKGSALYDSIFSACQAAGFDPQVIQLAPQLTSAINLVAS 237 Query: 244 GLGISIIPASMRHVELESVSYLGIDDAPDLKAPLHLAYRDSGQSGAVKRFIAEARKCAKA 303 GLGISI+P ++ V+L V Y+ I + P +A L LAY QS A RF A ++ + Sbjct: 238 GLGISIVPEAIEQVQLAQVRYVEIVE-PAPRAQLSLAYHR--QSIAASRFAAHLQRSLRR 294 Query: 304 AG 305 AG Sbjct: 295 AG 296 >gi|300312961|ref|YP_003777053.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 300 Score = 155 bits (391), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 100/300 (33%), Positives = 164/300 (54%), Gaps = 8/300 (2%) Query: 4 IDLRLLRYFIAVAEEGHLTKAAQRIGIQQPPLSQQIRALEKELGVTLFNRLPRGMELTES 63 ++LR L+YF AVA ++AA+ + I QPPLS+QIR LE+ LGV L +R R + LT + Sbjct: 1 MELRHLKYFNAVASTLSFSRAAELLHIAQPPLSRQIRQLEEMLGVELIDRASRPIALTMA 60 Query: 64 GQALLTDARQILAQVDSTVAGVRRIARGELGRIAVGFTESASLHPFVPTVIRAFRDAVPD 123 G+ Q+L++++ G R IARG +GF SA L+ +P +I+ FR +P+ Sbjct: 61 GKFFYEQTLQVLSRLEQIEEGTRVIARGRQHWFNIGFVPSA-LYGLLPEMIKRFRTEMPE 119 Query: 124 VALTVEESSTTDLVALMSQNRIDVAFIRSPVGDVSDITIETMMVEEMIVALPAAHPLV-R 182 + + E T + V + RIDV F R P+ D DI ET++ E ++ P HPL+ R Sbjct: 120 IEIGFSEIVTMEQVEALKSGRIDVGFGRLPISD-PDIVCETIVEEPLVAVFPLGHPLLAR 178 Query: 183 NKRRKSVALAALAEEVFILNRRPSGPGLYDTIIAACRAAGFSPRVEQEARKNLSTLSLVA 242 NK V LA LA + FIL P D ++ + P + +EA + + + LVA Sbjct: 179 NK----VTLAQLAAQRFILYPARPRPSYADQVLDIFSSRALRPSIVKEANEMQTAIGLVA 234 Query: 243 AGLGISIIPASMRHVELESVSYLGIDDAPDLKAPLHLAYRDSGQSGAVKRFIAEARKCAK 302 AG+G++++P S++ + V Y + + + +P+ + +R + +S ++R A A+ Sbjct: 235 AGVGVTLVPRSVQGLHRSDVVYRPLSNK-GVHSPVIMNFRANDRSLLLERLRTTAATIAR 293 >gi|300313102|ref|YP_003777194.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 300 Score = 148 bits (373), Expect = 7e-37, Method: Compositional matrix adjust. Identities = 91/268 (33%), Positives = 143/268 (53%), Gaps = 5/268 (1%) Query: 4 IDLRLLRYFIAVAEEGHLTKAAQRIGIQQPPLSQQIRALEKELGVTLFNRLPRGMELTES 63 I+LR L YF VAE H +AA+ + I QPPL++QI ALEKELG LF+R R ++LT + Sbjct: 2 IELRHLTYFRTVAETLHFGRAAELLHISQPPLTRQIAALEKELGAQLFDRSKRAIQLTAA 61 Query: 64 GQALLTDARQILAQVDSTVAGVRRIARGELGRIAVGFTESASLHPFVPTVIRAFRDAVPD 123 G+ D +I ++ V + G+ G + VGF S + + +P+V R + A P+ Sbjct: 62 GKYFYRDTTEIFKALERAKRNVSSSSTGKSGALKVGFMMSTAYN-ILPSVTRHYSAAYPE 120 Query: 124 VALTVEESSTTDLVALMSQNRIDVAFIRSPVGDVSDITIETMMVEEMIVALPAAHPLVRN 183 V + + E L + +IDV + P D + T+ E ++ LP H R Sbjct: 121 VDMRLTEYVPNLLATDIEDEKIDVGIMYRP-EDCRRLDSHTIYAEPLLAVLPRDH---RL 176 Query: 184 KRRKSVALAALAEEVFILNRRPSGPGLYDTIIAACRAAGFSPRVEQEARKNLSTLSLVAA 243 R+S++ A LAE+ FI R P +YD I C+ GF PR+ E + ++LV Sbjct: 177 AGRQSISAAELAEDAFISIPRMIAPVVYDLIQQHCQLHGFRPRIVLETNLQQTIVNLVGE 236 Query: 244 GLGISIIPASMRHVELESVSYLGIDDAP 271 GLG++++P SM+ + L++ + + AP Sbjct: 237 GLGVALVPTSMQAMHLDTTVFKPLIGAP 264 >gi|300309864|ref|YP_003773956.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 306 Score = 143 bits (360), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 93/291 (31%), Positives = 157/291 (53%), Gaps = 8/291 (2%) Query: 4 IDLRLLRYFIAVAEEGHLTKAAQRIGIQQPPLSQQIRALEKELGVTLFNRLPRGMELTES 63 +DL +R F+ A E + +AA + + QPPLS+QI LE LGV LF R+ R + LT Sbjct: 2 LDLAQVRCFVVAATELNFRRAAALLNMTQPPLSRQIHLLEHRLGVQLFERVGRTVRLTTE 61 Query: 64 GQALLTDARQILAQVDSTVAGVRRIARGELGRIAVGFTESASLHPFVPTVIRAFRDAVPD 123 G+ L DA ++L + + VRR ++G+ GR+ VGFT +A + +P ++ R ++P+ Sbjct: 62 GRVFLADASRLLQLSEQAESTVRRASQGKTGRVRVGFTGAAG-YELIPELLMQARTSLPE 120 Query: 124 VALTVEESSTTDLVALMSQNRIDVAFIRSPVGDVSDITIETMMVEEMIVALPAAHPLVRN 183 + + + E + + + N ID+ F+R PV + ++ + E +++A+P H L Sbjct: 121 IDVVLFELISVAQLEAFTANTIDIGFLR-PVASRQPLELQLVDEEALVLAMPQGHALAAY 179 Query: 184 KRRKSVALAALAEEVFILNRRPSGPGLYDTIIAACRAAGFSPRVEQEARKNLSTLSLVAA 243 R +A L + FI++ G Y+ I+A AA P Q ++ + LSLV A Sbjct: 180 DR---IATTQLDGQPFIMHSPTHGKYFYERIMAMLGAADVKPDFTQYIDQSHTILSLVRA 236 Query: 244 GLGISIIPASMRHVELESVSYLGI-DDAPDLKAPLHLAYRDSGQSGAVKRF 293 GLG+ I+PAS + + V + + D+AP +A + +A+R + AV F Sbjct: 237 GLGVGILPASAQRFHYDRVEFRPLADEAP--RAEMSMAWRPDQDNPAVTAF 285 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 904,263 Number of extensions: 37485 Number of successful extensions: 286 Number of sequences better than 1.0e-08: 66 Number of HSP's gapped: 246 Number of HSP's successfully gapped: 66 Length of query: 307 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 222 Effective length of database: 1,217,395 Effective search space: 270261690 Effective search space used: 270261690 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)