BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0026.p (401 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312742|ref|YP_003776834.1| outer membrane efflux protein [... 582 e-167 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 175 5e-45 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 174 8e-45 gi|300311360|ref|YP_003775452.1| outer membrane drug efflux lipo... 174 1e-44 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 173 2e-44 >gi|300312742|ref|YP_003776834.1| outer membrane efflux protein [Herbaspirillum seropedicae SmR1] Length = 492 Score = 582 bits (1499), Expect = e-167, Method: Compositional matrix adjust. Identities = 312/389 (80%), Positives = 346/389 (88%) Query: 2 SRVRQAEANARIAGAPLLPDADFAGSANRALPLSGGTPTTTASGMLEINYEVDFWGKNRA 61 SRV+QAEA ARIAGAPLLP A A R +P+SGGTPT TA+ L+++YE+DFWGKNRA Sbjct: 102 SRVKQAEAQARIAGAPLLPSAGINAGAAREIPISGGTPTNTANASLQVSYEIDFWGKNRA 161 Query: 62 GLAAAEASLRANMYDRETVALTVTSGIVSTFLQVLSLRDRLEIARQNVGNAQRVLKLVEA 121 G+ AAEA+LRAN YDRETVALTVTSGIVST+LQVLSLRDRL IARQNV NA+RVL+LVEA Sbjct: 162 GVEAAEATLRANRYDRETVALTVTSGIVSTYLQVLSLRDRLAIARQNVQNAERVLRLVEA 221 Query: 122 QSRAGAASPLDLARQRSALANQQAALPDLMQQERDAQTALAILLGRPPQNFSVGEKGLAT 181 QSRAG+ASPLDLARQRSALANQ+ +PDL QQE+DAQTALAILLGRPPQNF V EKGLA Sbjct: 222 QSRAGSASPLDLARQRSALANQRQLIPDLQQQEQDAQTALAILLGRPPQNFKVEEKGLAR 281 Query: 182 IQMPKIAPGLPSELLTRRPDIRRAEARLAEANANVAVARAALFPSIRLTGSTGAQSNALL 241 IQMP+I+ GLPSELLTRRPDIRRAEA LA ANANVAVARAALFPSI LTGSTGAQS+ALL Sbjct: 282 IQMPRISAGLPSELLTRRPDIRRAEASLAAANANVAVARAALFPSITLTGSTGAQSSALL 341 Query: 242 SLFDGPNLLANLGASLTAPIFDAGRLNSRRDLAVAQKQELVQVYRSAVVSALSEVEKSLG 301 SLFDGPNL ANLGA L APIFD G+L +RDLA+AQK+ELVQVYR V+++LSEVEK+LG Sbjct: 342 SLFDGPNLFANLGAGLIAPIFDGGKLRGQRDLAIAQKEELVQVYRQRVITSLSEVEKALG 401 Query: 302 AIRTLEERYRLKNQEVDQARFAFELSEIRYRSGAEDLMVVLDTQRILSEAQNQLGQIKLQ 361 AIR+LEERYRLK EVDQARFAFEL+EIRYR+GAEDL+VVLDTQR LSEAQNQLGQIKLQ Sbjct: 402 AIRSLEERYRLKVGEVDQARFAFELAEIRYRAGAEDLLVVLDTQRTLSEAQNQLGQIKLQ 461 Query: 362 RLQATVSLYKALGGGWRDDVRYGNPGTDS 390 RLQATVSLYKALGGGW+D G+ GT S Sbjct: 462 RLQATVSLYKALGGGWQDSQAPGDSGTSS 490 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 175 bits (444), Expect = 5e-45, Method: Compositional matrix adjust. Identities = 131/335 (39%), Positives = 180/335 (53%), Gaps = 18/335 (5%) Query: 50 NYEVDFWGKNRAGLAAAEASLRANMYDRETVALTVTSGIVSTFLQVLSLRDRLEIARQNV 109 ++E+D WG+ R+ AA S RA+ R VAL++ + + +L++ L +RLEIA Q + Sbjct: 149 SWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLEIANQTI 208 Query: 110 GNAQRVLKLVEAQSRAGAASPLDLARQRSALANQQAALPDLMQQERDAQTALAILLGRPP 169 + Q ++ + GA S L+L + + L QA L QQ AL +L+G P Sbjct: 209 ASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTLLVGSP- 267 Query: 170 QNFSVGEK--GLATIQMPKIAPGLPSELLTRRPDIRRAEARLAEANANVAVARAALFPSI 227 VG+ GL + P + PGLP+ LLTRRPD+ AE RL ANAN+ ARAA FPSI Sbjct: 268 VVLPVGQARAGLDAVFAP-LQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAAFFPSI 326 Query: 228 RLTGSTGAQSNALLSLFDGPNLLANLGASLTAPIFDAGRLNS-------RRDLAVAQKQE 280 LTG+ G S L LF + S+T PIF AGRL + RRDLAVA ++ Sbjct: 327 SLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVAAYEK 386 Query: 281 LVQVYRSAVVSALSEVEKSLGAIRTLEERYRLKNQEVDQARFAFELSEIRYRSGAEDLMV 340 VQ +A EV +L A + L ++ + V R LS +RY +GA + Sbjct: 387 TVQ-------TAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLD 439 Query: 341 VLDTQRILSEAQNQLGQIKLQRLQATVSLYKALGG 375 VLD QR L AQ QL QI+ L ++V+LY ALGG Sbjct: 440 VLDAQRDLLSAQQQLVQIRRALLSSSVALYAALGG 474 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 174 bits (442), Expect = 8e-45, Method: Compositional matrix adjust. Identities = 129/390 (33%), Positives = 209/390 (53%), Gaps = 17/390 (4%) Query: 4 VRQAEANARIAGAPLLPDADFAGSANRALPLSG----GTPTTTAS---GMLEINYEVDFW 56 + +A A +IA A LLP+ + +G+A R + G PT + S G+ YE+DF+ Sbjct: 92 IEEARAQYQIARADLLPNLNLSGNAARTRTAASQSLTGQPTISNSYQVGLAVPAYELDFF 151 Query: 57 GKNRAGLAAAEASLRANMYDRETVALTVTSGIVSTFLQVLSLRDRLEIARQNVGNAQRVL 116 G+ R AA AS + +++ +++ + + +L S ++L++A + + + Q L Sbjct: 152 GRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNL 211 Query: 117 KLVEAQSRAGAASPLDLARQRSALANQQAALPDLMQQERDAQTALAILLGR-----PPQN 171 L + + GA++ LD ++ + + + A L +Q A+ AL +L+G+ PP Sbjct: 212 DLAQKRLDVGASTALDFRLTQTLVESARVAKTQLERQRAQAENALTLLVGKKIGELPPAQ 271 Query: 172 FSVGEKGLATIQMPKIAPGLPSELLTRRPDIRRAEARLAEANANVAVARAALFPSIRLTG 231 EK + I GLPS+LLT RPDIR AE L ANAN+ ARAA FP I LTG Sbjct: 272 LLSDEKIVT-----DIPAGLPSDLLTNRPDIRSAEQSLLAANANIGAARAAFFPRISLTG 326 Query: 232 STGAQSNALLSLFDGPNLLANLGASLTAPIFDAGRLNSRRDLAVAQKQELVQVYRSAVVS 291 + G+ S+ L LF+ + + G SL+ PIFD GR ++ DLA +K + V Y A+ + Sbjct: 327 NFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQT 386 Query: 292 ALSEVEKSLGAIRTLEERYRLKNQEVDQARFAFELSEIRYRSGAEDLMVVLDTQRILSEA 351 A EV +L A +L+E+ + + + + +L+++R+++G + LD QR L A Sbjct: 387 AFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSA 446 Query: 352 QNQLGQIKLQRLQATVSLYKALGGGWRDDV 381 Q L Q + RL + LYKALGGG ++ Sbjct: 447 QQALIQARQLRLNNAIDLYKALGGGLNENT 476 >gi|300311360|ref|YP_003775452.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 470 Score = 174 bits (442), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 123/374 (32%), Positives = 199/374 (53%), Gaps = 3/374 (0%) Query: 3 RVRQAEANARIAGAPLLPDADFAGSANRALPLSGGTPTTTAS-GMLEINYEVDFWGKNRA 61 ++R+A+A A +A +P + A+ + L G +T AS ++++YEVD WG+ A Sbjct: 88 KLRRAQAQAGLADDAFVPALSASLGASSSKQLDGDRISTRASQSSVKLSYEVDLWGRLAA 147 Query: 62 GLAAAEASLRANMYDRETVALTVTSGIVSTFLQVLSLRDRLEIARQNVGNAQRVLKLVEA 121 + A DR++ ALT+ + + L R+ A Q++ A++ L+LV Sbjct: 148 ASDLSRWEALATEQDRQSTALTLIGTTANLYWTGAYLNQRVATAEQSIAYAEKTLELVRT 207 Query: 122 QSRAGAASPLDLARQRSALANQQAALPDLMQQERDAQTALAILLGRPPQNFSVGEKGLAT 181 Q +AGA S +++ + L +QQ++ +L+QQ + ALAIL +PP L Sbjct: 208 QYQAGAVSSVEVLSAQQNLYSQQSSYTELVQQRVENDNALAILFDQPPGKAIALPARLPE 267 Query: 182 IQMPKIAPGLPSELLTRRPDIRRAEARLAEANANVAVARAALFPSIRLTGSTGAQSNALL 241 +P +A G+P+ELL RRPD+R E R+ A A V AR +P + LTG+ G S+ALL Sbjct: 268 QALPVLAAGVPAELLGRRPDLRALELRIRSALAGVDQARLNYYPQLSLTGTLGTSSDALL 327 Query: 242 SLFDGPNLLANLGASLTAPIFDAGRLNSRRDLAVAQKQELVQVYRSAVVSALSEVEKSLG 301 L P +A LG +LT P + + ++ A V +R + +A SEVE +L Sbjct: 328 RLVQNP--VATLGGALTLPFLQHSEMKFKVGISQADFDTAVLNFRKGLYTAFSEVENALS 385 Query: 302 AIRTLEERYRLKNQEVDQARFAFELSEIRYRSGAEDLMVVLDTQRILSEAQNQLGQIKLQ 361 A + + L + +D A+ A L E+RYR+G+ L + LD Q A+ L Q++L Sbjct: 386 ARQQYARQQALLVRNLDAAQAAERLLEVRYRAGSATLQLWLDQQDKRRTAEISLAQVRLN 445 Query: 362 RLQATVSLYKALGG 375 +L+ ++LY+ALGG Sbjct: 446 QLKNQMTLYQALGG 459 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 173 bits (438), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 138/389 (35%), Positives = 205/389 (52%), Gaps = 14/389 (3%) Query: 2 SRVRQAEANARIAGAPLLPDADFAGSANR--------ALPLSGGT-PTTTASGMLEINYE 52 +R+ QA A R A A P D A R LP T P T + ++YE Sbjct: 106 ARLGQARALTRDARAGYFPQVDAGVGATRQRPSPASQGLPADADTRPNTLYRAQVNVSYE 165 Query: 53 VDFWGKNRAGLAAAEASLRANMYDRETVALTVTSGIVSTFLQVLSLRDRLEIARQNVGNA 112 VD +G+ + + AA A + + +V L + + + + V L + R V Sbjct: 166 VDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQYFLVRELDAAAALYRGTVDLR 225 Query: 113 QRVLKLVEAQSRAGAASPLDLARQRSALANQQAALPDLMQQERDAQTALAILLGRPPQNF 172 Q L+LV+ + G S +D+AR R+ LA+ Q+ + +Q A+ ALAILLGR P F Sbjct: 226 QESLRLVQRRFDEGDISEVDVARARAELASAQSEALGIARQRATAEHALAILLGRTPAEF 285 Query: 173 SVGEKGLATIQMPKIAPGLPSELLTRRPDIRRAEARLAEANANVAVARAALFPSIRLTGS 232 S+ + L + + + PGLPS LL RRPDI AE +A ANA V VA++A FPS+ LTG Sbjct: 286 SLPPQPLTRLAV-TVPPGLPSALLERRPDIAAAERAMAAANARVGVAKSAFFPSLNLTGG 344 Query: 233 TGAQSNALLSLFDGPN---LLANL-GASLTAPIFDAGRLNSRRDLAVAQKQELVQVYRSA 288 G +S L LF+ + LL L G +L+ P+FD GR + D A A +E V YR Sbjct: 345 LGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVASYRGT 404 Query: 289 VVSALSEVEKSLGAIRTLEERYRLKNQEVDQARFAFELSEIRYRSGAEDLMVVLDTQRIL 348 V++A EVE +L +R L+++ R +++ V + A +LS +YR GA V+D R + Sbjct: 405 VLNAFREVEDNLANLRILDQQNRAQDEAVAASARAAKLSHTQYREGAVSYFSVIDADRTV 464 Query: 349 SEAQNQLGQIKLQRLQATVSLYKALGGGW 377 + Q + +R ++TV+L +ALGGGW Sbjct: 465 LQQQRASVTLDGERARSTVNLIRALGGGW 493 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.130 0.352 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,085,878 Number of extensions: 40480 Number of successful extensions: 257 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 224 Number of HSP's successfully gapped: 19 Length of query: 401 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 314 Effective length of database: 1,207,925 Effective search space: 379288450 Effective search space used: 379288450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)