BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0021.p (468 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312639|ref|YP_003776731.1| choline dehydrogenase [Herbaspi... 518 e-148 gi|300313761|ref|YP_003777853.1| gluconate dehydrogenase [Herbas... 405 e-114 gi|300313846|ref|YP_003777938.1| choline dehydrogenase-like prot... 375 e-105 >gi|300312639|ref|YP_003776731.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 593 Score = 518 bits (1334), Expect = e-148, Method: Compositional matrix adjust. Identities = 241/367 (65%), Positives = 290/367 (79%) Query: 4 ITKPKVDVVLVGMGWSGSIMGMEMTEAGLSVVGLERGETRDTNPDFAYPKIADELTYAQR 63 I K KVDVV+VG+GW+GSIMGME+TEAGLSV+ LERG+ RDTN D YPK DEL Y+ R Sbjct: 3 IKKDKVDVVMVGLGWTGSIMGMELTEAGLSVLALERGDMRDTNTDTPYPKAVDELKYSVR 62 Query: 64 YKLMQNLSKETVTVRHSPNDDALPYRQLGSFLLGNGLGGAGVHWNGVTWRAYDAELQMRS 123 L Q+LS+ETVT+RH+ +D A+PYRQ GSFLLG+G+GGAG HWNG+ +R EL+MR+ Sbjct: 63 GALFQDLSRETVTIRHTLSDVAVPYRQHGSFLLGDGVGGAGFHWNGMMYRNLPEELEMRT 122 Query: 124 HYEQRYGKRFIPVDMTIQDWPMTYAELEPFYDRFEYVAGVSGKAGNLDGKIIEGGNVFEA 183 H+EQ+YGK+FIP DMTIQD +TY ELEPFYD E V VSGKAGNL G+I+ GGN E Sbjct: 123 HHEQKYGKKFIPEDMTIQDHGVTYKELEPFYDFSEKVMAVSGKAGNLKGQIVPGGNPLEG 182 Query: 184 PRKREYPLPPLKNNAPATLFETAAREVGYHPFPIPAANASQAYQNPYGMQLGPCNYCGYC 243 R E+P PPL+ A+L E A R+VGYHP+P PAANASQ + NPYG+++GPCNYCG+C Sbjct: 183 ERSDEFPTPPLQYQYSASLVEKAMRDVGYHPYPAPAANASQPFTNPYGVRIGPCNYCGFC 242 Query: 244 ERFGCYMYAKASPQTCILPALLKKPNFELRDKSYVTRINLAGDGKRATGVTYIDAQGRSV 303 E +GCYMY+KASPQT ILP LLK+ NFE+R K+ V ++NL GKRATGVTYIDAQGR V Sbjct: 243 ENYGCYMYSKASPQTTILPVLLKRKNFEVRTKAQVIKVNLDASGKRATGVTYIDAQGREV 302 Query: 304 EQPADLVILCAYQMHNVRLLLLSGIGKPYDPKTGDGVVGKNYAYQKNSSVSLFFDKDVAI 363 EQPADLV+L A+Q HNVRLLLLSGIGKPYDP TG+GVVGKN+AYQ N +++ K Sbjct: 303 EQPADLVLLTAFQTHNVRLLLLSGIGKPYDPVTGEGVVGKNFAYQYNGGINVIMPKGTQF 362 Query: 364 NPFAGAG 370 NPF G G Sbjct: 363 NPFIGTG 369 >gi|300313761|ref|YP_003777853.1| gluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 593 Score = 405 bits (1041), Expect = e-114, Method: Compositional matrix adjust. Identities = 206/418 (49%), Positives = 260/418 (62%), Gaps = 7/418 (1%) Query: 1 MADITKPKVDVVLVGMGWSGSIMGMEMTEAGLSVVGLERGETRDTNPDFAYPKIADELTY 60 MA+ VDVV+VG GW+G+IM EMTEAG VV LERG RDT PD AYPK +EL Y Sbjct: 1 MANKNMKPVDVVIVGFGWTGAIMAKEMTEAGQRVVALERGVYRDTYPDGAYPKTINELEY 60 Query: 61 AQRYKLMQNLSKETVTVRHSPNDDALPYRQLGSFLLGNGLGGAGVHWNGVTWRAYDAELQ 120 QR+KL QNL+K + T R D A+PYRQ+ F G G+GGAG+HW+G WR EL+ Sbjct: 61 QQRFKLFQNLNKSSFTFRRKTGDSAIPYRQIAMFKPGEGVGGAGLHWSGCHWRILPEELR 120 Query: 121 MRSHYEQRYGKRFIPVDMTIQDWPMTYAELEPFYDRFEYVAGVSGKAGNLDGKII-EGGN 179 MRSHYE+RYGK FIP DMT+QDW ++Y ELEP++D E + G SG A + GK++ E GN Sbjct: 121 MRSHYEERYGKNFIPKDMTLQDWGVSYEELEPYFDFAEKMMGTSGTAYRVGGKVVDESGN 180 Query: 180 VFEAPRKREYPLPPLKNNAPATLFETAAREVGYHPFPIPAANASQAYQNPYGMQLGPCNY 239 FEA R +PLP K A LF AA++ G HPF +P+ANA+ Y N YG Q+GPC + Sbjct: 181 PFEADRSDNFPLPAQKEQYQAALFRKAAQQAGLHPFTLPSANAAAPYVNQYGCQMGPCTF 240 Query: 240 CGYCERFGCYMYAKASPQTCILPALLKKPNFELRDKSYVTRINLAGDGKRATGVTYIDAQ 299 CG+C + CY Y+KASP I+PAL + FELR V RI L K+ATGVTY+D Sbjct: 241 CGFCSGYACYNYSKASPNVNIMPALRQSNLFELRSSCNVLRIELDSSRKKATGVTYVDIN 300 Query: 300 GRSVEQPADLVILCAYQMHNVRLLLLSGIGKPYDPKTGDGVVGKNYAYQKNSSVSLFFDK 359 G +V QPA +VI + +N RL LLSGIGKPYDP + G VG+N A+Q S+++ FF+ Sbjct: 301 GDTVFQPASIVIASTFAYNNARLFLLSGIGKPYDPVSNTGAVGRNIAFQMMSTINAFFEP 360 Query: 360 DVAINPFAGAGXPAARCSTISTPTISTMARTALSAAPT------SARWSPAAVPSGNP 411 IN F GAG + + + +P S S AVPSG P Sbjct: 361 GKNINGFIGAGGNGVAVDDFNGDHMDHGPLGFVGGSPIWCNPSGSKPISGIAVPSGTP 418 >gi|300313846|ref|YP_003777938.1| choline dehydrogenase-like protein [Herbaspirillum seropedicae SmR1] Length = 590 Score = 375 bits (964), Expect = e-105, Method: Compositional matrix adjust. Identities = 200/449 (44%), Positives = 269/449 (59%), Gaps = 8/449 (1%) Query: 1 MADITKPKVDVVLVGMGWSGSIMGMEMTEAGLSVVGLERGETRDTNPDFAYPKIADELTY 60 M DIT VDVV+VG+GW+GS+M +E+ +AGL V LERG R DFAYPK AD+ Y Sbjct: 1 MPDITNDDVDVVIVGLGWAGSLMAIELAQAGLKVRALERGPDRPPE-DFAYPKPADQYGY 59 Query: 61 AQRYKLMQNLSKETVTVRHSPNDDALPYRQLGSFLLGNGLGGAGVHWNGVTWRAYDAELQ 120 A R K+ +TVR++ ALP R+ G+F G G+GG+G+HW V RA +L+ Sbjct: 60 ATRNKVFATPRDAALTVRYNTAQQALPTRKWGAFAPGTGVGGSGLHWTAVLIRATPTDLK 119 Query: 121 MRSHYEQRYGKRFIPVDMTIQDWPMTYAELEPFYDRFEYVAGVSGKAGNLDGKIIEGGNV 180 ++++ +Q Y + +M I+D+P T+ E+EP YD F+ V G+SG GNL G+I GG+ Sbjct: 120 LKTYADQAYKHSNLDPEMRIKDFPFTWNEIEPHYDFFDKVCGLSGTTGNLRGQIQPGGDP 179 Query: 181 FEAPRKREYPLPPLKNNAPATLFETAAREVGYHPFPIPAANASQAYQNPYGMQLGPCNYC 240 FE PR YPLP L + TLF AR++GYHPFP P+AN SQA+ NPYG Q+ PCNYC Sbjct: 180 FEGPRSNPYPLPALHDTYNNTLFADVARKLGYHPFPNPSANVSQAWTNPYGAQIAPCNYC 239 Query: 241 GYCERFGCYMYAKASPQTCILPALLKKPNFELRDKSYVTRINLAGDGKRATGVTYIDAQG 300 G+C ++ C Y+KASPQT IL + + PNF+ + + V R++L D K A GVTY+D Sbjct: 240 GFCSKYPCLNYSKASPQTTILDVVKRLPNFDYKVNANVIRVDLHADKKTARGVTYVDENM 299 Query: 301 RSVEQPADLVILCAYQMHNVRLLLLSGIGKPYDPKTGDGVVGKNYAYQKNSSVSLFFDKD 360 + V QPA +VIL ++Q NVRL+LLSGIGKPYDP T GV+G+NYA+ N +LFF KD Sbjct: 300 KEVFQPAKIVILSSFQFANVRLMLLSGIGKPYDPITETGVIGRNYAFLSNGGATLFF-KD 358 Query: 361 VAINPFAGAGXPAARCSTISTPTISTMARTAL--SAAPTSARWSPA----AVPSGNPSCR 414 N FA AG + IS + A S++ S A A+P G PS Sbjct: 359 KHFNSFATAGATGEMFNDISPGNFDDGVKLGFIGGAKIHSSQASGAPIGTALPPGTPSWG 418 Query: 415 LTRPNGAAAGRRRSRTITCTRSTSVRKGR 443 G S I+ T + +G Sbjct: 419 RGWKEGMIDWYDHSMKISITTTCMSYRGH 447 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,467,399 Number of extensions: 61847 Number of successful extensions: 222 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 226 Number of HSP's successfully gapped: 3 Length of query: 468 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 379 Effective length of database: 1,198,455 Effective search space: 454214445 Effective search space used: 454214445 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)