BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0020.p (235 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312640|ref|YP_003776732.1| hypothetical protein Hsero_3343... 229 1e-61 gi|300313760|ref|YP_003777852.1| hypothetical protein Hsero_4478... 190 8e-50 gi|300313845|ref|YP_003777937.1| hypothetical protein Hsero_4563... 157 1e-39 >gi|300312640|ref|YP_003776732.1| hypothetical protein Hsero_3343 [Herbaspirillum seropedicae SmR1] Length = 240 Score = 229 bits (584), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 124/232 (53%), Positives = 156/232 (67%), Gaps = 8/232 (3%) Query: 4 RRGFLFKTIAIVPAASAL---TTAALLPSA-AQTADVAVGADYQPRFFNADEWTFLKAAV 59 RR FL + +AIVPAAS L TT A+ +A A +++ Y P +F A EW FL AAV Sbjct: 8 RRRFLRQVLAIVPAASTLGGATTVAVSQAAMADSSEPGKPRAYTPTYFTAAEWAFLNAAV 67 Query: 60 DRLIPADPEGPGALELNVAAFIDGQMESGFGHASNWYMQGPFKGDAPAPKNXFGYQASMP 119 D LIP+D G GALE V FID QME+ +G WYMQGPF D+ P+ GYQ S+ Sbjct: 68 DHLIPSDQYGAGALETGVPEFIDRQMETPYGAGKLWYMQGPFHPDS-VPE--LGYQLSLV 124 Query: 120 PRELYRAGIAALNEHCRKNFSGKTFAQLAPAEQEQLFKDMDGGTLKFEQVSAQWFFTFLL 179 PR++YR GIAA N+ C KN GK FA+L A+Q ++ K MDGG + FE V A+ FF F+L Sbjct: 125 PRDIYRQGIAACNDWCSKN-HGKVFAELDKAKQLEVLKMMDGGKISFETVPAKTFFNFML 183 Query: 180 QNTKEGYLSDPIHGGNKGMASWKMIGFPGARADFLDFVGPSDKVYPYGPVSL 231 NTKEGY +DP++GGN+ M WKM+GFPGARADF D+V + YPYGPVS+ Sbjct: 184 GNTKEGYFADPMYGGNQKMGGWKMVGFPGARADFADWVDQYNVKYPYGPVSI 235 >gi|300313760|ref|YP_003777852.1| hypothetical protein Hsero_4478 [Herbaspirillum seropedicae SmR1] Length = 235 Score = 190 bits (483), Expect = 8e-50, Method: Compositional matrix adjust. Identities = 104/232 (44%), Positives = 132/232 (56%), Gaps = 9/232 (3%) Query: 4 RRGFLFKTIAIVPAASALTTAALLPSAAQTADVAVGADYQPRFFNADEWTFLKAAVDRLI 63 RR FL + P A+ TA L + A+ + YQP FFN DE+ L A D LI Sbjct: 10 RRRFLRGALTAAPVAALAVTAGSLTAKAEGP--SPDQPYQPSFFNRDEFALLSALADTLI 67 Query: 64 PADPEGPGALELNVAAFIDGQMESGFGHASNWYMQGPFKGDAPAPKNXFGYQASMPPREL 123 PAD GPGAL+ V FID QM + +G WYMQGPF DAP FGYQ PPREL Sbjct: 68 PADDTGPGALQAGVPEFIDAQMNTPYGQGQLWYMQGPFTPDAPP---EFGYQLPYPPREL 124 Query: 124 YRAGIAALNEHCRKNFSGKTFAQLAPAEQEQL---FKDMDGGTLKFEQVSAQWFFTFLLQ 180 Y+ G+A + R+ + G + +L ++E++ F+ + G+LK + F LLQ Sbjct: 125 YKKGLAEFDAAIRQQY-GHGYVELNTQQREKMMAEFEKGEQGSLKMATIPPSALFNLLLQ 183 Query: 181 NTKEGYLSDPIHGGNKGMASWKMIGFPGARADFLDFVGPSDKVYPYGPVSLG 232 N EGY DP GGN+GM SWKMIGFPGARAD+ D+V K YP P+S G Sbjct: 184 NVHEGYFCDPTDGGNRGMGSWKMIGFPGARADYYDWVDQYGKSYPLPPISRG 235 >gi|300313845|ref|YP_003777937.1| hypothetical protein Hsero_4563 [Herbaspirillum seropedicae SmR1] Length = 237 Score = 157 bits (396), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 89/200 (44%), Positives = 120/200 (60%), Gaps = 12/200 (6%) Query: 42 YQPRFFNADEWTFLKAAVDRLIPADPEGPGALELNVAAFIDGQMESGFGHASNWYMQGPF 101 Y+P FFN +W F+ AA DRLIPA+ GPGALE NV FID Q+ +G G S+ Y QGP Sbjct: 44 YKPVFFNPAQWLFILAACDRLIPAEGRGPGALETNVPVFIDQQLHAGLG--SDIYQQGPH 101 Query: 102 KGDAPAPKNXFGYQASMPPRELYRAGIAALNEHCRKNFSGKTFAQLAPAEQEQLFKDMDG 161 + DAPA G+Q P+++YR GI L + + GK F QL +++ L + Sbjct: 102 QPDAPA---TLGFQIPYAPQDVYRIGIT-LTQQISQQQHGKPFEQLDAKDKDALLTALQK 157 Query: 162 GTLKF-----EQVSAQWFFTFLLQNTKEGYLSDPIHGGNKGMASWKMIGFPGARADFLDF 216 + F + + FF LL +TK GYL+DP++GGNK M +W IGFPGARA + ++ Sbjct: 158 NGVDFAALGEKSMRPSQFFGQLLGDTKNGYLADPMYGGNKDMKAWVAIGFPGARAAYTEW 217 Query: 217 VGPSDKVYPYGPVSL-GKRS 235 V + YP GPVSL GKR+ Sbjct: 218 VDQHNVKYPLGPVSLSGKRA 237 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 779,514 Number of extensions: 33671 Number of successful extensions: 75 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 68 Number of HSP's successfully gapped: 3 Length of query: 235 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 153 Effective length of database: 1,231,600 Effective search space: 188434800 Effective search space used: 188434800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)