BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0017.p (260 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herb... 443 e-126 gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogen... 127 1e-30 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 112 4e-26 gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herb... 112 4e-26 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 111 5e-26 >gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 443 bits (1140), Expect = e-126, Method: Compositional matrix adjust. Identities = 209/261 (80%), Positives = 243/261 (93%), Gaps = 1/261 (0%) Query: 1 MNTSLKDQRVVVTAGAQGIGLAITASFVEAGAHVHICDVSDDFLDSARKQFAHAPVSYSK 60 M+ +LKDQ+V+VTAGAQGIGLAITA+FVEAGAHVHICDVS+DFL SAR +F HAPVSYS+ Sbjct: 1 MDLNLKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDVSEDFLASARARFGHAPVSYSR 60 Query: 61 TDVANEAQVDAMFAELAQRW-GTLDVLVNNAGIAGPTAKVEETELSAWDQTIAVNLTGPF 119 TDV++E +VDAMFA+LAQRW G LDVL+NNAGIAGPT+ VEE LS WDQT+AVNLTGPF Sbjct: 61 TDVSSEREVDAMFADLAQRWSGRLDVLINNAGIAGPTSPVEEVALSDWDQTLAVNLTGPF 120 Query: 120 LCTRRAVPLLKKAGGGSIVNISSAAGRLGFPLRTPYSASKFGVIGLTESWAMELGPANIR 179 LCTRRAVPLLKK GGG+IVNISS AGRLGF LRTPYSASK+GVIGLTE+WA+ELGP++IR Sbjct: 121 LCTRRAVPLLKKNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPSHIR 180 Query: 180 VNAILPGIVAGERQERVIAAKAASYNIGHEEMRERLLSKVSMRKMVTAQDIANQIIYICS 239 VNA+LPGIV G RQER++AAKAA+Y IGHEEMR+RLLS+VS+RKMVTA+DIANQ+I++CS Sbjct: 181 VNAVLPGIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLRKMVTAEDIANQVIFLCS 240 Query: 240 PAGSAISGQSLSVCGNVEHLG 260 PAG++ISGQSLSVCGNVE LG Sbjct: 241 PAGASISGQSLSVCGNVEVLG 261 >gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 127 bits (318), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 83/257 (32%), Positives = 136/257 (52%), Gaps = 13/257 (5%) Query: 5 LKDQRVVVTAGAQGIGLAITASFVEAGAHVHICDVSDDFLDSARKQFAHAPVSYSKT--- 61 LKD+ ++T A GIG I + GA V I D++ + A K A V T Sbjct: 3 LKDKVALITGSASGIGKEIAVEYARQGAKVVIADLALE----AAKATAEEIVKEGGTAMA 58 Query: 62 ---DVANEAQVDAMFAELAQRWGTLDVLVNNAGIAGPTAKVEETELSAWDQTIAVNLTGP 118 +V +E QVD A+ +G LD++++NAGI + V E L W + +A++L G Sbjct: 59 VAMNVTDETQVDKGVADAVNTYGGLDIMISNAGIQ-IISPVAELSLENWRKMLAIHLDGA 117 Query: 119 FLCTRRAVP-LLKKAGGGSIVNISSAAGRLGFPLRTPYSASKFGVIGLTESWAMELGPAN 177 FL TR A+ ++K+ GGSI+ + S L PL+ PY +K G++GL ++ A E Sbjct: 118 FLTTRAAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDK 177 Query: 178 IRVNAILPGIVAGERQERVIAAKAASYNIGHEEMRERLLSKVSMR-KMVTAQDIANQIIY 236 IR N I PG V E+ I +A + I E++ ++++ K ++ + T QD+A ++ Sbjct: 178 IRTNVICPGFVRTPLVEKQIPEQAKEFGISEEDVIKKIMLKDTVDGEFTTTQDVAQTAVF 237 Query: 237 ICSPAGSAISGQSLSVC 253 + + +A++GQS+ V Sbjct: 238 LAAFPTNALTGQSVVVS 254 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 112 bits (279), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 90/261 (34%), Positives = 129/261 (49%), Gaps = 22/261 (8%) Query: 1 MNTSLKDQRV-VVTAGAQGIGLAITASFVEAGAHVHICDVSDDFLDSARKQFAHAPVSYS 59 MN + RV +VT GA GIG +I+ G V + D++ LD+A K A A V+ Sbjct: 1 MNRNDSGARVALVTGGAMGIGASISQQLAAKGHTVLVADIN---LDAASKT-ADALVAAG 56 Query: 60 ------KTDVANEAQVDAMFAELAQRWGTLDVLVNNAGIAGPTAKVEETELSAWDQTIAV 113 + D+ + + A FA + + +G DVLVNNAG+A T + L W QT+ V Sbjct: 57 HRAAPLQMDLGSPESIAAAFAHIEKDYGRCDVLVNNAGVA-RTYSFLDYPLENWLQTMNV 115 Query: 114 NLTGPFLCTRRAVPLLKKAGGGSIVNISSAAGRLGFPLRTPYSASKFGVIGLTESWAMEL 173 N+TG L + A L+ K G IVNISS +G RT Y SK VIGLT A+EL Sbjct: 116 NVTGVLLAGQHAARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIEL 175 Query: 174 GPANIRVNAILPGIVAGERQERVIAAKAASYNIGHEEMRERLLSKVSMRKMVTAQDIANQ 233 I VN++ PG V + + + E+ RE V MR+ T +IA+ Sbjct: 176 AQYGITVNSVAPGPVDTPMTQAMHS----------EQTRESYYRLVPMRRYGTTDEIASA 225 Query: 234 IIYICSPAGSAISGQSLSVCG 254 + Y+ + + I+G + V G Sbjct: 226 VCYLAAEESAYITGHVIPVDG 246 >gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 112 bits (279), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 73/251 (29%), Positives = 124/251 (49%), Gaps = 12/251 (4%) Query: 4 SLKDQRVVVTAGAQGIGLAITASFVEAGAHVHICDVSDDFLDSARKQFAHAPVS--YSKT 61 SL +RVV+T G GIG A+ +FV GA V D++ + + AP++ + Sbjct: 16 SLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIAAEPSQALVASLKDAPIAPRFFPC 75 Query: 62 DVANEAQVDAMFAELAQRWGTLDVLVNNAGIAGPTAKVEETELSAWDQTIAVNLTGPFLC 121 ++ N + A F E+ G +D+L+NNA K + + WD+ +AVNL F C Sbjct: 76 NLMNLEALRATFTEIETVMGGVDILINNAA-NDDRHKTADVTPAYWDERLAVNLRHQFFC 134 Query: 122 TRRAVPLLKKAGGGSIVNISSAAGRLGFPLRTPYSASKFGVIGLTESWAMELGPANIRVN 181 + +P +++ G I+N S + LG P T Y +K G+ G+T A + G +RVN Sbjct: 135 AQAVLPGMRERKQGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTHGMARDFGRDGVRVN 194 Query: 182 AILPGIVAGERQERVIAAKAASYNIGHEEMRERLLSKVSMRKMVTAQDIANQIIYICSPA 241 AI+PG + RQ + ++ EE ++L+ + V D+A +++ S + Sbjct: 195 AIIPGAIRTPRQTLL-------WHTPEEE--AKILAAQCLPTRVDPHDVAALALFLSSDS 245 Query: 242 GSAISGQSLSV 252 G+ +G+ V Sbjct: 246 GAKCTGREYYV 256 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 111 bits (278), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 77/252 (30%), Positives = 123/252 (48%), Gaps = 8/252 (3%) Query: 5 LKDQRVVVTAGAQGIGLAITASFVEAGAHVHICDVSD--DFLDSARKQFAHAPVSYSKTD 62 L+D+ +VT G G G I ++ GA + + D+ + + A ++K D Sbjct: 3 LQDKVAIVTGGGSGFGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAVFAKAD 62 Query: 63 VANEAQVDAMFAELAQRWGTLDVLVNNAGIAGPTAKVEETELSAWDQTIAVNLTGPFLCT 122 V+ A +D + A + +G LD++VNNAG + E E +D+ AVN+ FL Sbjct: 63 VSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFLSA 122 Query: 123 RRAVPLLKKAGGGSIVNISSAAGRLGFPLRTPYSASKFGVIGLTESWAMELGPANIRVNA 182 + VP ++ GGG+ +NI+S AG P T Y+ SK VI ++S A ELGP IRVN Sbjct: 123 KTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRVNC 182 Query: 183 ILPGIVAGERQERVIAAKAASYNIGHEEMRERLLSKVSMRKMVTAQDIANQIIYICSPAG 242 + P I A + N R++ ++ + M + T +DIAN +Y+ S Sbjct: 183 VNPVISATGLLSEFMGVPDTPEN------RKKFVATIPMGRFSTPEDIANACLYLGSDEA 236 Query: 243 SAISGQSLSVCG 254 ++G + V G Sbjct: 237 EFVTGVCIEVDG 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 774,723 Number of extensions: 30830 Number of successful extensions: 254 Number of sequences better than 1.0e-08: 50 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 50 Length of query: 260 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 177 Effective length of database: 1,226,865 Effective search space: 217155105 Effective search space used: 217155105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)