BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0006.p (257 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312619|ref|YP_003776711.1| ABC transporter permease [Herba... 463 e-132 gi|300310817|ref|YP_003774909.1| nitrate/sulfonate/bicarbonate A... 87 1e-18 gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate A... 81 1e-16 gi|300310541|ref|YP_003774633.1| nitrate transporter permease [H... 79 3e-16 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 79 4e-16 >gi|300312619|ref|YP_003776711.1| ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 257 Score = 463 bits (1191), Expect = e-132, Method: Compositional matrix adjust. Identities = 234/257 (91%), Positives = 245/257 (95%) Query: 1 MSSQRRVTGYRKKFAMVLAIAFILLMWQIAAWSLPDFLMPGVPTVLARLWNEVQTPAFRI 60 MSSQRRVTG RKK AMVLAI FILLMWQIAAWSLP FLMPGVP+V ARLW EVQ+ FR+ Sbjct: 1 MSSQRRVTGIRKKLAMVLAIVFILLMWQIAAWSLPSFLMPGVPSVAARLWEEVQSSNFRL 60 Query: 61 ALWGSLTRLGGGYGAALLLGIGFGLIGAVLFFFREVLKSAIIILQSIPSIAWVPLFLILM 120 AL+GSL RLG GYG+AL+LGIGFGL+GAVLFFFREVLKSAI+ILQSIPSIAWVPLFLILM Sbjct: 61 ALFGSLYRLGMGYGSALVLGIGFGLVGAVLFFFREVLKSAIVILQSIPSIAWVPLFLILM 120 Query: 121 GFGNLPIIVVVAIAAFFPAALSVMNATESVQQVHVSAARVMGASRLGMLRRVYLPAVMPE 180 GFGNLPI+VVVAIAAFFPAALSVMNATESVQQVHVSAARVMGASRL MLRRVYLPAVMPE Sbjct: 121 GFGNLPIVVVVAIAAFFPAALSVMNATESVQQVHVSAARVMGASRLSMLRRVYLPAVMPE 180 Query: 181 LITGAQLAFGNAWRALISAEMLIGFGKGLGRTLAYSGETADMVGVMTNILTIAILAALID 240 LITGAQLAFGNAWRALISAEML+GFGKGLGRTLAYSGETADMVGVM NILTIAILAALID Sbjct: 181 LITGAQLAFGNAWRALISAEMLVGFGKGLGRTLAYSGETADMVGVMANILTIAILAALID 240 Query: 241 QVILENLKHRLLRYQYV 257 QVILENLKHRLL+YQYV Sbjct: 241 QVILENLKHRLLKYQYV 257 >gi|300310817|ref|YP_003774909.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 256 Score = 87.4 bits (215), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 67/250 (26%), Positives = 124/250 (49%), Gaps = 1/250 (0%) Query: 5 RRVTGYRKKFAMVLAIAFILLMWQIAAWSLPDFLMPGVPTVLARLWNEVQTPAFRIALWG 64 RR+ R V+AI L++WQ+ + + +P V A + + ++ Sbjct: 7 RRLKAARPFLISVIAIVGGLVLWQLVSLTTSPLFLPSVSMTWAAALELISDGTLQQSVLA 66 Query: 65 SLTRLGGGYGAALLLGIGFGLIGAVLFFFREVLKSAIIILQSIPSIAWVPLFLILMGFGN 124 S R+ G+GA +++G+ G+ R++L I + IP IA+V L +I +G G Sbjct: 67 SSGRILAGWGAGVVIGVPLGIFMGRFETVRQLLDPYIEFFRFIPPIAFVTLAVIWLGPGE 126 Query: 125 LPIIVVVAIAAFFPAALSVMNATESVQQVHVSAARVMGASRLGMLRRVYLPAVMPELITG 184 L I ++ F L+ + ++V + + AA +GA + +L V LP+ +P ++TG Sbjct: 127 LSKIALIFYTTVFIVTLNTIAGVQAVNPLRLRAAASLGAGQWKILSTVILPSTVPFMVTG 186 Query: 185 AQLAFGNAWRALISAEMLIGFGKGLGRTLAYSGETADMVGVMTNILTIAILAALIDQVIL 244 A++A GN++ ++SAE ++ +GLG + + + V I+ + IL L D+V+ Sbjct: 187 ARIAMGNSFLTVVSAE-IVSAREGLGALIWTARNFSRTEWVFVGIIALGILGYLFDRVLR 245 Query: 245 ENLKHRLLRY 254 + L RY Sbjct: 246 VATRKLLARY 255 >gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 273 Score = 80.9 bits (198), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 47/153 (30%), Positives = 88/153 (57%), Gaps = 2/153 (1%) Query: 103 ILQSIPSIAWVPLFLILMGFGNLPIIVVVAIAAFFPAALSVMNATESVQQVHVSAARVMG 162 +++ IP IA++PL ++ G GN P + ++++ AFFP ++ + V +++ AAR +G Sbjct: 118 VIRPIPPIAYIPLAILWFGLGNPPALFLISLGAFFPVLMNTIAGVRQVDSIYLRAARNLG 177 Query: 163 ASRLGMLRRVYLPAVMPELITGAQLAFGNAWRALISAEMLIGFGKGLGRTLAYSGETADM 222 AS+ + RV LPA +P +++G ++ G A+ +I AEM I GLG + + E Sbjct: 178 ASQSTLFLRVMLPAAVPYILSGVRIGIGTAFIVVIVAEM-IAVSNGLGFRIMEAREYFWS 236 Query: 223 VGVMTNILTIAILAALIDQVILENLKHRLLRYQ 255 ++ ++TI +L ID + + L + +LR+ Sbjct: 237 DKIIAGMITIGLLGLAID-LGMSRLNNYMLRWH 268 >gi|300310541|ref|YP_003774633.1| nitrate transporter permease [Herbaspirillum seropedicae SmR1] Length = 307 Score = 79.0 bits (193), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 50/147 (34%), Positives = 85/147 (57%), Gaps = 3/147 (2%) Query: 65 SLTRLGGGYGAALLLGIGFGL-IGAVLFFFREVLKSAIIILQSIPSIAWVPLFLILMGFG 123 SL R+G G+G A L+GI G IG V F R + I +L+ + +AW+P+ L++ Sbjct: 116 SLQRVGTGFGLAALVGIPLGFAIGRVEFLAR-MFGPIISLLRPVSPLAWLPIGLLVFKSA 174 Query: 124 NLPIIVVVAIAAFFPAALSVMNATESVQQVHVSAARVMGASRLGMLRRVYLPAVMPELIT 183 N I + I + +P ++ + V Q +++ ARV+ S +L ++ P+V+P ++T Sbjct: 175 NPAAIWSIFICSIWPMIINTAVGVQRVPQDYMNVARVLNLSEWKVLTKILFPSVLPYMLT 234 Query: 184 GAQLAFGNAWRALISAEMLIGFGKGLG 210 G +L+ G AW +++AEML G G G+G Sbjct: 235 GVRLSIGTAWLVIVAAEMLTG-GVGIG 260 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 78.6 bits (192), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 61/216 (28%), Positives = 105/216 (48%), Gaps = 8/216 (3%) Query: 6 RVTGYRKKFAMVLAIAFILLMWQIAAWSL-PDFLMPGVPTVLARLWNEVQTPAFRIALWG 64 R TG+ ++ +AF+LL+ A L P L+P + LW+ Q + Sbjct: 25 RFTGW------IVPLAFLLLVEAGARLELIPARLLPPPSELAHTLWSLAQRGELLPHIAA 78 Query: 65 SLTRLGGGYGAALLLGIGFGLIGAVLFFFREVLKSAIIILQSIPSIAWVPLFLILMGFGN 124 SL R+ G+ L + GL+ + +L+ + L++IPS+AW+PL L+ +G Sbjct: 79 SLARVAAGFVIGSALAVALGLLVGISRRAEALLEPSFQALRAIPSLAWIPLLLLWLGIDE 138 Query: 125 LPIIVVVAIAAFFPAALSVMNATESVQQVHVSAARVMGASRLGMLRRVYLPAVMPELITG 184 P +V++AI AFFP L+++ + + G + ++ + LPA +P L G Sbjct: 139 TPKVVLIAIGAFFPVYLNLVAGIRDIDHKLIEVGTTSGLNTQALIGHILLPASLPYLFAG 198 Query: 185 AQLAFGNAWRALISAEMLIGFGKGLGRTLAYSGETA 220 + AW L++AE LI KG+G L+ E++ Sbjct: 199 LRGGLSLAWMFLVAAE-LIAATKGVGYLLSDGRESS 233 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.142 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 634,507 Number of extensions: 21074 Number of successful extensions: 153 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 14 Length of query: 257 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 174 Effective length of database: 1,226,865 Effective search space: 213474510 Effective search space used: 213474510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 129 (54.3 bits)