BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0066.p (329 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310490|ref|YP_003774582.1| major facilitator superfamily p... 525 e-150 gi|300310476|ref|YP_003774568.1| major facilitator superfamily t... 470 e-134 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 228 6e-61 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 214 8e-57 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 203 1e-53 >gi|300310490|ref|YP_003774582.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 525 bits (1352), Expect = e-150, Method: Compositional matrix adjust. Identities = 255/323 (78%), Positives = 289/323 (89%) Query: 1 MAFVHNETSFYILRFLLGVAEAGFFPGVVYYFTQWLPNSERGKAMAVFLSGSAIASILSG 60 MAFV +ETSFY +RFLLGVAEAGFFPGVVYYFTQWLPN ERG+AMAVFLSGSAIASILSG Sbjct: 106 MAFVQSETSFYFMRFLLGVAEAGFFPGVVYYFTQWLPNHERGRAMAVFLSGSAIASILSG 165 Query: 61 PISGALLQIEGGGLHXWQWMFIIEGLASVLLCFVVWRRLDSRPQDAAWLSDDERNSLAAI 120 PI+GALL IEGGGLH WQWMFIIEG AS++ C VVW LDS P++A WLS ++R +LAA+ Sbjct: 166 PITGALLLIEGGGLHGWQWMFIIEGGASMVFCVVVWLVLDSTPREAKWLSQEQREALAAV 225 Query: 121 IAHEQRERQASKPVHVSSWSLLKDPQILLFCFIYFAIQLTIYGATFWLPSIIKKMGHFND 180 IA EQ +R A++P HVS+ SLL+DPQI+LFCFIYFAIQLTIYGATFWLP+IIKKMGHFND Sbjct: 226 IAAEQEQRAANRPAHVSALSLLRDPQIVLFCFIYFAIQLTIYGATFWLPTIIKKMGHFND 285 Query: 181 FQVGLFNSIPWIISIVAMYIFASLAARFRHQQAWVAATLIIAAAGMFASTTGGPVFAFVA 240 FQVGLFNSIPWI++I AMY+FA LAAR++ Q WVA TL+I+AAGM S GPV FVA Sbjct: 286 FQVGLFNSIPWIVAIAAMYLFAVLAARYKKPQFWVATTLVISAAGMAISAVAGPVIGFVA 345 Query: 241 ICFAGIGFKAASSLFWPIPQAYLDARIAAAVIALVNSIGNLGGFFAPATFGYLEQKTGSI 300 +CFAGIGFKAASSLFWPIPQAYLDARIAAAV+AL+NS+GNLGGFFAPATFGYLEQKTGSI Sbjct: 346 VCFAGIGFKAASSLFWPIPQAYLDARIAAAVLALINSLGNLGGFFAPATFGYLEQKTGSI 405 Query: 301 QGGLYALAAASVIAAGLVFLART 323 QGGL+AL+ AS++AA LVFLART Sbjct: 406 QGGLFALSGASLLAAVLVFLART 428 >gi|300310476|ref|YP_003774568.1| major facilitator superfamily transporter protein [Herbaspirillum seropedicae SmR1] Length = 449 Score = 470 bits (1209), Expect = e-134, Method: Compositional matrix adjust. Identities = 242/323 (74%), Positives = 277/323 (85%) Query: 1 MAFVHNETSFYILRFLLGVAEAGFFPGVVYYFTQWLPNSERGKAMAVFLSGSAIASILSG 60 MAFV++ET+FY+LRFLLG AEAGFFPGV+YYFTQWLP SERG+AMA+FLSGSA+ASILSG Sbjct: 118 MAFVNSETTFYVLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSGSALASILSG 177 Query: 61 PISGALLQIEGGGLHXWQWMFIIEGLASVLLCFVVWRRLDSRPQDAAWLSDDERNSLAAI 120 PISG LLQI+GGGL WQWMFIIEG+ASVLLC VW LDS P DA WL+ ER+++ Sbjct: 178 PISGGLLQIDGGGLQGWQWMFIIEGMASVLLCGFVWFWLDSMPADAKWLTAQERSAITRA 237 Query: 121 IAHEQRERQASKPVHVSSWSLLKDPQILLFCFIYFAIQLTIYGATFWLPSIIKKMGHFND 180 I EQ ER +P VS LL+DPQIL+FCFIYF+I LTIYGATFWLPSII+KMG F+D Sbjct: 238 IVDEQAERMKHQPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFWLPSIIRKMGSFSD 297 Query: 181 FQVGLFNSIPWIISIVAMYIFASLAARFRHQQAWVAATLIIAAAGMFASTTGGPVFAFVA 240 FQVGLFNSIPW+ISIVAMY FA+LAARF+HQQAW A L+IAAAGMFAST G V+AFV+ Sbjct: 298 FQVGLFNSIPWLISIVAMYAFAALAARFKHQQAWAATALVIAAAGMFASTFGNSVYAFVS 357 Query: 241 ICFAGIGFKAASSLFWPIPQAYLDARIAAAVIALVNSIGNLGGFFAPATFGYLEQKTGSI 300 ICFA +GFKAASSLFWP+PQAYLDARIAA VIAL+NSIGNLGGF AP FG+LEQ+TGSI Sbjct: 358 ICFAAVGFKAASSLFWPLPQAYLDARIAAGVIALINSIGNLGGFVAPTAFGFLEQRTGSI 417 Query: 301 QGGLYALAAASVIAAGLVFLART 323 QGGL LA S++AAG+VFLAR+ Sbjct: 418 QGGLMGLAITSLVAAGVVFLARS 440 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 228 bits (580), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 127/323 (39%), Positives = 186/323 (57%), Gaps = 9/323 (2%) Query: 1 MAFVHNETSFYILRFLLGVAEAGFFPGVVYYFTQWLPNSERGKAMAVFLSGSAIASILSG 60 MA+V FYI+RFLLGVAEAGFFPGV+ Y T W P + RGK A+F++G A++ ++ G Sbjct: 109 MAYVTTPEMFYIMRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGG 168 Query: 61 PISGALLQIEGG--GLHXWQWMFIIEGLASVLLCFVVWRRLDSRPQDAAWLSDDERNSLA 118 P+SG ++ G G WQWMFI+E + S++L +V + R +DA WLS++E+ L Sbjct: 169 PLSGWIMHAMPGVWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDADWLSEEEKRLLE 228 Query: 119 AIIAHEQRERQASKPVHVSSWSLLKDPQILLFCFIYFAIQLTIYGATFWLPSIIKKMGHF 178 A I+HE+ +++ H+S ++ + ++ L IYF + +YG +FWLP+IIK G Sbjct: 229 ANISHEESQKE-----HLSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVS 283 Query: 179 NDFQVGLFNSIPWIISIVAMYIFASLAARFRHQQAWVAATLIIAAAGMFASTTGG--PVF 236 + VGL +IP+ + AM + A R ++ VA I G+ ST G Sbjct: 284 DPLNVGLLTAIPYAFAAAAMVLIGRSADARRERRWHVAIPAFIGCIGLLLSTQYGHNTTL 343 Query: 237 AFVAICFAGIGFKAASSLFWPIPQAYLDARIAAAVIALVNSIGNLGGFFAPATFGYLEQK 296 A ++ A +G LFW +P A+L AAA IAL+NS+GNL GF +P G+L+ Sbjct: 344 AMASLTLASVGILTTLPLFWSLPTAFLSGTAAAAGIALINSLGNLAGFVSPFLVGWLKDA 403 Query: 297 TGSIQGGLYALAAASVIAAGLVF 319 T S G+Y LAA+ VI L Sbjct: 404 TQSTNAGMYVLAASLVIGGLLTL 426 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 214 bits (545), Expect = 8e-57, Method: Compositional matrix adjust. Identities = 117/320 (36%), Positives = 183/320 (57%), Gaps = 14/320 (4%) Query: 3 FVHNETSFYILRFLLGVAEAGFFPGVVYYFTQWLPNSERGKAMAVFLSGSAIASILSGPI 62 FV N T FYILRFLLG+AEAGF+PG++ Y T W P+ R K +AVF+SG +A IL P+ Sbjct: 112 FVQNATQFYILRFLLGLAEAGFYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPL 171 Query: 63 SGALLQI--EGGGLHXWQWMFIIEGLASVLLCFVVWRRLDSRPQDAAWLSDDERNSLAAI 120 SG ++ + GGL WQWMF+IE + +VL+ LD+ + A WL+D+E+ SL A Sbjct: 172 SGWIMDAFHQNGGLEGWQWMFLIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQAD 231 Query: 121 IAHEQRERQASKPVHVSSWSLLKDPQILLFCFIYFAIQLTIYGATFWLPSIIKKMGHFND 180 I + + +++ +++KD ++ L C IYF+ + YG T W+P+++K G + Sbjct: 232 IDGDAKGKESKHGFG----AIVKDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGN 287 Query: 181 FQVGLFNSIPWIISIVAMYIFASLAARFRHQQAWVAATLIIAAAG-----MFASTTGGPV 235 ++GL ++IP+ +I+AM + A R R ++ + ++ G +FA T Sbjct: 288 LEIGLLSAIPFGCAIIAMNLIGRSADRMRERRWHLVIPALMGGVGFVGAALFADNTA--- 344 Query: 236 FAFVAICFAGIGFKAASSLFWPIPQAYLDARIAAAVIALVNSIGNLGGFFAPATFGYLEQ 295 + ++ A G + LFW +P A+L AA IA +NS+GNL GF +P GYL+ Sbjct: 345 VSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKD 404 Query: 296 KTGSIQGGLYALAAASVIAA 315 T + G+Y LA V+ + Sbjct: 405 LTHNNATGMYMLAVMLVVGS 424 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 203 bits (517), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 129/322 (40%), Positives = 185/322 (57%), Gaps = 8/322 (2%) Query: 1 MAFVHNETSFYILRFLLGVAEAGFFPGVVYYFTQWLPNSERGKAMAVFLSGSAIASILSG 60 MA+V TSFYILRFLLG+ EAGFFPGVV Y T W P R + +F++ AIA + G Sbjct: 113 MAYVTTPTSFYILRFLLGIFEAGFFPGVVLYLTYWFPAQRRARVNGLFMTSFAIAGAVGG 172 Query: 61 PISGALLQ-IEG-GGLHXWQWMFIIEGLASVLLCFVVWRRLDSRPQDAAWLSDDERNSLA 118 PI+G ++ +EG G L WQW+FI+EG+ S+L F V L RP +A WLS+ E+N++ Sbjct: 173 PIAGLIMSGMEGVGHLANWQWLFILEGIPSILAGFAVLAWLPERPANAKWLSEAEKNAVT 232 Query: 119 AIIAHEQRERQASKPVHVSSWSLLKDPQILLFCFIYFAIQLTIYGATFWLPSIIKKMGHF 178 ++A E ++ H S + ++ + +YF + FW PSIIK++G Sbjct: 233 QVLAAE----NSAPGKHFSFKDACTNYRVWICAAVYFCVVSGNATIAFWAPSIIKEIGVK 288 Query: 179 NDFQVGLFNSIPWIISIVAMYIFASLAARFRHQQAWVAATLIIAAAGMFAS--TTGGPVF 236 + Q+GL ++IP+I +AM + + ++ A +IA G+ A+ T G Sbjct: 289 GNLQIGLISAIPFIAGTLAMIWNGFHSDKTGERRLHSAIATLIACIGLVATGMTLGNATL 348 Query: 237 AFVAICFAGIGFKAASSLFWPIPQAYLDARIAAAVIALVNSIGNLGGFFAPATFGYLEQK 296 A ++ A IG AA +FW IP A+L AA IAL+NSIGNL GF AP G L+ Sbjct: 349 ALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTS 408 Query: 297 TGSIQGGLYALAAASVIAAGLV 318 TGS+ GLY +AA ++A+ LV Sbjct: 409 TGSLSSGLYFVAALELVASFLV 430 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.139 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 992,655 Number of extensions: 39875 Number of successful extensions: 259 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 235 Number of HSP's successfully gapped: 14 Length of query: 329 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 243 Effective length of database: 1,212,660 Effective search space: 294676380 Effective search space used: 294676380 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)