BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0029.0050.p
         (543 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312545|ref|YP_003776637.1| iron-regulated membrane protein...   674   0.0  

>gi|300312545|ref|YP_003776637.1| iron-regulated membrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 546

 Score =  674 bits (1739), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 343/516 (66%), Positives = 405/516 (78%), Gaps = 4/516 (0%)

Query: 1   MREGFRQSMAWLHTWSGLLVCWVLFLVFCAGTASYFREEITLWMKPELHASHSAQISDAQ 60
           M+EG RQSMAWLHTWSGLLVCW+L LVF  G+ASYF++EI+ WM PELHA+    +    
Sbjct: 1   MKEGLRQSMAWLHTWSGLLVCWLLLLVFAGGSASYFKDEISFWMMPELHATQRQPVPQPL 60

Query: 61  AAQTALTALQSKAPDASRWFITLPAERSTALRIGWISPPPKDAPPKT-RGKFTSQIIDPL 119
           A + A+  LQ +AP A RWFI+LP+ RS A+R+GWI+PPPKD P  + R +FTS+++D  
Sbjct: 61  AVERAIAHLQQQAPQAERWFISLPSARSVAMRVGWIAPPPKDGPAGSARSRFTSELVDVT 120

Query: 120 TGEKMPDVRETRGGEFLYRLHFDLHYMPALWARWIVGFCAMFMLVAIVSGVITHKRIFKD 179
           TG+KM  VRETRGGEFLYRLHFDLHYMP  WARWIV   A+FMLVAIVSGVITHKRIFKD
Sbjct: 121 TGQKMAAVRETRGGEFLYRLHFDLHYMPVQWARWIVSIGALFMLVAIVSGVITHKRIFKD 180

Query: 180 FFTFRPKKGQRSWLDAHNATAVLALPYHLMITYTGLVTLMFMYMPWGIQTAY--KGNQES 237
           FFTFR  KGQRSWLDAHNA AVLALP+HLMITYTGLVTLMFM MPWG+QTAY  KG + +
Sbjct: 181 FFTFRANKGQRSWLDAHNAAAVLALPFHLMITYTGLVTLMFMIMPWGVQTAYPGKGGESA 240

Query: 238 FFADVFPS-ARNGKPAGKPMPLTPLQPLLAQAEQRWHGIPAVGITINHPGDANATIQIRR 296
           FF +VFP+ A  GK AG+  PLT + PL+ QA+Q W G P   +TINHPGDA AT+ + R
Sbjct: 241 FFEEVFPNVAGRGKAAGQAAPLTAIGPLVTQAQQHWQGAPLALVTINHPGDAKATVVLTR 300

Query: 297 QEEKGLNYIQPSVQFNGVTGALVSADNEDIGGANATRGVLYGLHIGHFANPLLRVLFFLC 356
           Q+   L+Y+QP++ F+GV+G   ++  E       TR VLYGLH+GHFA+P LR LFFLC
Sbjct: 301 QKAATLSYLQPALVFDGVSGVQKASVGEQPSNTGVTRAVLYGLHLGHFADPWLRWLFFLC 360

Query: 357 GLSGCAMVATGALLWAVKTRQGYARKLAAGGRVGFGLRLVEALNIGAIAGLPIAFAAYFW 416
           GLSG  MVA+GALLWAVKTRQ  A+ +A G R G+GLRLV+ALNIGAIAGLPIAFA+YFW
Sbjct: 361 GLSGYLMVASGALLWAVKTRQKEAKAIAQGARPGWGLRLVDALNIGAIAGLPIAFASYFW 420

Query: 417 ANRLLPVAMPRRPDAEISWFFIAWGVAAVLAQIRPDRRMWQWQLWTGALLFMGIPVLNVF 476
           ANRLLP+ M +RP AEI+ FFIAW VAAVLAQ+RP   MW+ QL  GALLF GI VLN+F
Sbjct: 421 ANRLLPLEMTQRPQAEINVFFIAWAVAAVLAQLRPGLAMWRVQLGAGALLFAGIVVLNIF 480

Query: 477 TTSSHLGVTLFMAQGPWSVAGFDLTVLALGIGLALA 512
           TT SHLGVTL + +GPW VAGFDLT+LALG  L LA
Sbjct: 481 TTHSHLGVTLLLGRGPWPVAGFDLTMLALGASLWLA 516


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.325    0.137    0.445 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,712,841
Number of extensions: 72528
Number of successful extensions: 257
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 254
Number of HSP's successfully gapped: 1
Length of query: 543
Length of database: 1,619,870
Length adjustment: 90
Effective length of query: 453
Effective length of database: 1,193,720
Effective search space: 540755160
Effective search space used: 540755160
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 133 (55.8 bits)