BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0030.p (295 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 368 e-103 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 149 4e-37 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 137 1e-33 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 135 4e-33 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 135 4e-33 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 368 bits (945), Expect = e-103, Method: Compositional matrix adjust. Identities = 179/253 (70%), Positives = 207/253 (81%) Query: 43 MNRFEDKVVIVTGAASGMGKATAHRFSKEGAKVVLADRQHEKLQETLEELPADRTAARLT 102 + RFE KVVIVTGAASG+G+ATA RFS EGA+V+LADR L + + LP +RTAAR T Sbjct: 9 LQRFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTAARET 68 Query: 103 DVSAHEQVRDLIDFAIEKFGKLDVLVNDAGIHVPGNVLEVSVDDWHRVQANNVDSVFFGA 162 DVS HEQVR L+DFAIE+FG+LDVLV+DAG+ GNV EVS +DWHRVQA NV+ VF+GA Sbjct: 69 DVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGVFYGA 128 Query: 163 REAIPHLAKTKGSIVNVASVSGLGGDWGMSAYDTSKGAVVNLTRVLALDFAKKGIRVNSV 222 REA+PHL KT+G IVNVASVSGL DW +SAY+ SKGAV NLTR +ALDF +KG+R+N+V Sbjct: 129 REALPHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRINAV 188 Query: 223 CPSFTYTAMTADMVDDKELMAKFAERIPLGRGAQPEEIASVIAFLASEDARFVTGVNLPV 282 CPS T+TAMTADM DD L+ KFAERI LGRGA P EIA+VI FLAS DA FV GVNLPV Sbjct: 189 CPSLTHTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASFVNGVNLPV 248 Query: 283 DGGLTASNGQPAQ 295 DGGL ASNGQP Q Sbjct: 249 DGGLMASNGQPPQ 261 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 149 bits (375), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 95/248 (38%), Positives = 144/248 (58%), Gaps = 7/248 (2%) Query: 45 RFEDKVVIVTGAASGMGKATAHRFSKEGAKVVLADRQHEKLQETLEELPA--DRTAARLT 102 R +DKV IVTG SG G+ A +++EGA +V+AD +EE+ A R Sbjct: 2 RLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAVFAKA 61 Query: 103 DVSAHEQVRDLIDFAIEKFGKLDVLVNDAGI-HVPGNVLEVSVDDWHRVQANNVDSVFFG 161 DVS + L+ A+E FGKLD++VN+AG H +LEV D++ RV A NV S+F Sbjct: 62 DVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFLS 121 Query: 162 AREAIPHLAKTKG-SIVNVASVSGLGGDWGMSAYDTSKGAVVNLTRVLALDFAKKGIRVN 220 A+ +P+ + G + +N+AS +G+ G++ Y+ SKGAV+ ++ +A + IRVN Sbjct: 122 AKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRVN 181 Query: 221 SVCPSFTYTAMTADMV---DDKELMAKFAERIPLGRGAQPEEIASVIAFLASEDARFVTG 277 V P + T + ++ + D E KF IP+GR + PE+IA+ +L S++A FVTG Sbjct: 182 CVNPVISATGLLSEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEAEFVTG 241 Query: 278 VNLPVDGG 285 V + VDGG Sbjct: 242 VCIEVDGG 249 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 137 bits (344), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 84/251 (33%), Positives = 139/251 (55%), Gaps = 6/251 (2%) Query: 43 MNRFED--KVVIVTGAASGMGKATAHRFSKEGAKVVLADRQHEKLQETLEELPA--DRTA 98 MNR + +V +VTG A G+G + + + + +G V++AD + +T + L A R A Sbjct: 1 MNRNDSGARVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAA 60 Query: 99 ARLTDVSAHEQVRDLIDFAIEKFGKLDVLVNDAGIHVPGNVLEVSVDDWHRVQANNVDSV 158 D+ + E + + +G+ DVLVN+AG+ + L+ +++W + NV V Sbjct: 61 PLQMDLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGV 120 Query: 159 FFGAREAIPHLAKTK-GSIVNVASVSGLGGDWGMSAYDTSKGAVVNLTRVLALDFAKKGI 217 + A + K + G IVN++S+SG+ G +AY TSK AV+ LTR +A++ A+ GI Sbjct: 121 LLAGQHAARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGI 180 Query: 218 RVNSVCPSFTYTAMTADMVDDKELMAKFAERIPLGRGAQPEEIASVIAFLASEDARFVTG 277 VNSV P T MT M ++ + +P+ R +EIAS + +LA+E++ ++TG Sbjct: 181 TVNSVAPGPVDTPMTQAM-HSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITG 239 Query: 278 VNLPVDGGLTA 288 +PVDGG A Sbjct: 240 HVIPVDGGFVA 250 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 135 bits (340), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 82/247 (33%), Positives = 132/247 (53%), Gaps = 3/247 (1%) Query: 46 FEDKVVIVTGAASGMGKATAHRFSKEGAKVVLADRQHEKLQETLEELPADRTAARLT--D 103 E K+ ++TG++ G+G A A ++ GA +VL R KL +L A+ + D Sbjct: 17 LEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLRAEGHTIHESSFD 76 Query: 104 VSAHEQVRDLIDFAIEKFGKLDVLVNDAGIHVPGNVLEVSVDDWHRVQANNVDSVFFGAR 163 ++ E+V+ + + G + +LVN+AGI + E DWH + + N++SVFF A+ Sbjct: 77 ATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFFAAQ 136 Query: 164 EAIPHLA-KTKGSIVNVASVSGLGGDWGMSAYDTSKGAVVNLTRVLALDFAKKGIRVNSV 222 H+ + G I+N+ SV G G++ Y +KGAV T+ +A+D+ K GI+VN + Sbjct: 137 AVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNGL 196 Query: 223 CPSFTYTAMTADMVDDKELMAKFAERIPLGRGAQPEEIASVIAFLASEDARFVTGVNLPV 282 P + T M +V+D + R P R E++ FLAS+ +RFV G L V Sbjct: 197 GPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHILYV 256 Query: 283 DGGLTAS 289 DGG+TA+ Sbjct: 257 DGGVTAT 263 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 135 bits (340), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 80/246 (32%), Positives = 137/246 (55%), Gaps = 7/246 (2%) Query: 46 FEDKVVIVTGAA--SGMGKATAHRFSKEGAKVVLADRQHEKLQETLEELPADRTAARLTD 103 +DKV I+TG A +G+G +TA + +GA++V+ D + + L A + D Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGAQHLGL-VAD 61 Query: 104 VSAHEQVRDLIDFAIEKFGKLDVLVNDAGIHVPGNVLEVSVDDWHRVQANNVDSVFFGAR 163 V+ +++FG++D+LVN+AG+ P +LE++ D+ R+ ++ + ++ Sbjct: 62 VTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYMSQ 121 Query: 164 EAIPHL-AKTKGSIVNVASVSGL--GGDWGMSAYDTSKGAVVNLTRVLALDFAKKGIRVN 220 +P + A+ GSIV ++SVS GG G Y +K V+ L R +A +F +GIRVN Sbjct: 122 AVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQGIRVN 181 Query: 221 SVCPSFTYTAMTADMVDDKELMAKFAERIPLGRGAQPEEIASVIAFLASEDARFVTGVNL 280 + P T + D++ E A+ A+ IPLGR P+++ V FLAS+ +++ TGV L Sbjct: 182 CITPGLISTDINKDILS-AEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTGVTL 240 Query: 281 PVDGGL 286 V+GG+ Sbjct: 241 DVNGGM 246 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 957,365 Number of extensions: 39991 Number of successful extensions: 331 Number of sequences better than 1.0e-08: 51 Number of HSP's gapped: 224 Number of HSP's successfully gapped: 51 Length of query: 295 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 210 Effective length of database: 1,217,395 Effective search space: 255652950 Effective search space used: 255652950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)