BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0003.p (558 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde deh... 897 0.0 gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde deh... 683 0.0 gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde deh... 458 e-130 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 218 1e-57 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 207 2e-54 >gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 503 Score = 897 bits (2317), Expect = 0.0, Method: Compositional matrix adjust. Identities = 447/498 (89%), Positives = 474/498 (95%) Query: 61 IQHYINGRIAPSESGRQQDVFNPATGEVSRQVVLASTAEVNAAVASASAAAPGWAETAPL 120 I H+ING+I+ SESGR QDVFNPATG + +V LAS EVNAAVA+ASAAAP WAETAPL Sbjct: 6 IDHFINGQISRSESGRSQDVFNPATGAATAKVALASVQEVNAAVAAASAAAPAWAETAPL 65 Query: 121 KRARVLFKFKELIEQHHDDLAAAITREHGKVFSDAKGEVTRGLEIVEFACGVPQLLKTQF 180 KRARVLFKFKELIEQ+HD LA AIT EHGKVFSDAKGEVTRGLEIVEFA G+PQLLKTQF Sbjct: 66 KRARVLFKFKELIEQNHDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLLKTQF 125 Query: 181 TDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPMAIATGNSFILKPSERDPTPSL 240 TDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAP+AIATGNSF+LKPSERDP+PSL Sbjct: 126 TDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERDPSPSL 185 Query: 241 MLADLFKRAGLPDGVFNVVQGDKVAVDALLQHPDVQAISFVGSTPIAEYIYSEATKRAGT 300 MLA+LFKRAGLPDGVFNVVQGDKVAVDALL+HP+VQA+SFVGSTPIAEYIY +AT+RAGT Sbjct: 186 MLAELFKRAGLPDGVFNVVQGDKVAVDALLEHPEVQAVSFVGSTPIAEYIYRKATERAGT 245 Query: 301 FPLRAQALGGAKNHLVVMPDANLEQAVDALIGAAYGSAGERCMAISVAVAVGSVADKLVE 360 FPLRAQALGGAKNHLVVMPDANL+QAVDALIGAAYGSAGERCMAISVAVAVGSVADKLVE Sbjct: 246 FPLRAQALGGAKNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVADKLVE 305 Query: 361 ALIPRVKALKIRNGMEADAEMGPLVTATHKAKVEGYIDDGVKAGAKLLVDGRGITVPGHE 420 ALIPRVKALK++NGME DAEMGPLVTA HKAKVEGYI GV AGAKLLVDGRG+ VPGHE Sbjct: 306 ALIPRVKALKVKNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAKLLVDGRGLKVPGHE 365 Query: 421 NGFFVGGTLFDNVTLDMKIYKEEIFGPVLCVVRVPDFAEAVRLINAHEYGNGVSLFTADG 480 GFF+GGTLFD+VT +MKIY+EEIFGPVLCVVRVPDFA AV+LINAHEYGNGVSLFTADG Sbjct: 366 EGFFIGGTLFDHVTTEMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSLFTADG 425 Query: 481 NTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGIKFYTRYKSIMQ 540 NTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGI+FYTRYKSIMQ Sbjct: 426 NTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGIRFYTRYKSIMQ 485 Query: 541 RWSATIGKGAEFTMPVAK 558 RWSATIGKGAEFTMPVAK Sbjct: 486 RWSATIGKGAEFTMPVAK 503 >gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 497 Score = 683 bits (1763), Expect = 0.0, Method: Compositional matrix adjust. Identities = 330/498 (66%), Positives = 386/498 (77%), Gaps = 4/498 (0%) Query: 61 IQHYINGRIAPSESGRQQDVFNPATGEVSRQVVLASTAEVNAAVASASAAAPGWAETAPL 120 I HYI G+ + SGR DVFNPA G QV L ++ EV+AAV + AA P W+ T PL Sbjct: 4 IAHYIGGQRVDTRSGRYADVFNPAQGVPVAQVALGTSDEVDAAVKAGVAAFPAWSNTPPL 63 Query: 121 KRARVLFKFKELIEQHHDDLAAAITREHGKVFSDAKGEVTRGLEIVEFACGVPQLLKTQF 180 RARVLF+F LI+Q DD A I REHGK FSDA+GEV RG+EIVEFA G+PQ+LK ++ Sbjct: 64 TRARVLFRFLHLIQQRADDFARIIVREHGKTFSDAQGEVARGIEIVEFAAGIPQMLKGEY 123 Query: 181 TDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPMAIATGNSFILKPSERDPTPSL 240 TD I GID W++RQ LGV AGITPFNFP MVP+WM P+AIA GN F+LKPSERDP+ SL Sbjct: 124 TDQIARGIDAWSMRQALGVVAGITPFNFPAMVPMWMFPIAIACGNCFVLKPSERDPSASL 183 Query: 241 MLADLFKRAGLPDGVFNVVQGDKVAVDALLQHPDVQAISFVGSTPIAEYIYSEATKRAGT 300 ++ADL K AGLPDGVFNVVQGDKV VDALL HP V+AISFVGSTPIAEYIY+ + + Sbjct: 184 LIADLLKEAGLPDGVFNVVQGDKVTVDALLDHPQVKAISFVGSTPIAEYIYARGSAKGK- 242 Query: 301 FPLRAQALGGAKNHLVVMPDANLEQAVDALIGAAYGSAGERCMAISVAVAVGSVADKLVE 360 R QALGGAKNH+VVMPDA+++ AVDAL+GAAYGSAGERCMAISVA+AVGS D+LV Sbjct: 243 ---RVQALGGAKNHMVVMPDADMQLAVDALMGAAYGSAGERCMAISVALAVGSAGDELVA 299 Query: 361 ALIPRVKALKIRNGMEADAEMGPLVTATHKAKVEGYIDDGVKAGAKLLVDGRGITVPGHE 420 AL R + LKI GM A AEMGP+VTA K ++EG I GV+ GA L+VDGRG VPG E Sbjct: 300 ALAERARKLKINEGMAAGAEMGPVVTAAAKERIEGLIGRGVEEGATLVVDGRGYQVPGFE 359 Query: 421 NGFFVGGTLFDNVTLDMKIYKEEIFGPVLCVVRVPDFAEAVRLINAHEYGNGVSLFTADG 480 GFFVGGTL D+VT +M +YKEEIFGPVLCV+R PD A AV LINAHEYGNGV+++T DG Sbjct: 360 KGFFVGGTLLDHVTPEMTVYKEEIFGPVLCVLRCPDIASAVELINAHEYGNGVAVYTRDG 419 Query: 481 NTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGIKFYTRYKSIMQ 540 A EF R+I+VGMVG+NVP+PVPMA+ SFGGWKRSLFGD H YG EG++FYTR K++MQ Sbjct: 420 GVAREFVRQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHHMYGPEGVRFYTRAKAVMQ 479 Query: 541 RWSATIGKGAEFTMPVAK 558 RW T GAEF P K Sbjct: 480 RWPNTASAGAEFAFPQMK 497 >gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 458 bits (1179), Expect = e-130, Method: Compositional matrix adjust. Identities = 231/481 (48%), Positives = 316/481 (65%), Gaps = 5/481 (1%) Query: 64 YINGRIAPSESGRQQDVFNPATGEVSRQVVLASTAEVNAAVASASAAAPGWAETAPLKRA 123 Y+NG S S +DV NPAT EV +V A+ EV+ AVA+A W T+ +R Sbjct: 12 YLNGEKVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKETFKTWRNTSLAQRM 71 Query: 124 RVLFKFKELIEQHHDDLAAAITREHGKVFSDAKGEVTRGLEIVEFACGVPQLLKTQFTDN 183 R++ KF++L+ ++ LA ITREHGK DA+GEV RGLE+VE AC + L + +N Sbjct: 72 RIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGELAEN 131 Query: 184 IGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPMAIATGNSFILKPSERDPTPSLMLA 243 + GG+D + L QPLGV AGIT FNFPVM+P +M P+A+A GN+F+LKPSE+DPT SL L Sbjct: 132 VAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDPTSSLFLV 191 Query: 244 DLFKRAGLPDGVFNVVQGDKVAVDALLQHPDVQAISFVGSTPIAEYIYSEATKRAGTFPL 303 +L +AGLP GV NVV G + + HPD++A+SF+GST + +IY +RA Sbjct: 192 ELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIY----RRASEAGK 247 Query: 304 RAQALGGAKNHLVVMPDANLEQAVDALIGAAYGSAGERCMAISVAVAVGSVADKLVEALI 363 RAQ + GAKNH +V+PDA +QA++ L+GAA+G+AG+RCMA SV V VG + + E ++ Sbjct: 248 RAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQTREWIPE-IV 306 Query: 364 PRVKALKIRNGMEADAEMGPLVTATHKAKVEGYIDDGVKAGAKLLVDGRGITVPGHENGF 423 R KA+K+ G + A++GPLV+ K +VE I GV+ GAKLL+DGR V G ENG Sbjct: 307 ERSKAMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAKLLLDGRNCKVAGSENGN 366 Query: 424 FVGGTLFDNVTLDMKIYKEEIFGPVLCVVRVPDFAEAVRLINAHEYGNGVSLFTADGNTA 483 FVG T+F V +M IY +EIFGP +C+V + EA+ INA+ GNG S+FT+ G A Sbjct: 367 FVGPTVFTGVKPEMDIYTQEIFGPAMCIVELDTLDEAIAFINANPNGNGTSIFTSSGYAA 426 Query: 484 HEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGIKFYTRYKSIMQRWS 543 +F I VG VGINVPIPVP+A+ SF G + S GD G++ + F+T+ K++ RW Sbjct: 427 RKFQNEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFWTQTKTVTARWY 486 Query: 544 A 544 A Sbjct: 487 A 487 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 218 bits (554), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 163/499 (32%), Positives = 249/499 (49%), Gaps = 30/499 (6%) Query: 54 MTAAPST-------IQH-YINGRIAPSESGRQQDVFNPATGEVSRQVVLASTAEVNAAVA 105 MT++P T + H YI+G+ P + + V PAT E ++ L + A+V+ AV Sbjct: 1 MTSSPLTHLTRNARLDHFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQ 60 Query: 106 SASAAAPGWAETAPLKRARVLFKFKELIEQHHDDLAAAITREHGKVFSDAKGE----VTR 161 +A A P WA T+PL+RA +L + L+ + + A A+T E G S A+ Sbjct: 61 AARRAIPAWAATSPLERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAE 120 Query: 162 GLEIVEFACGVPQLLKTQFTDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPMAI 221 L + ++ + T I +R+P+GV A ITP+N+P+ A+ Sbjct: 121 HLRVARDNLADYPFIRPRGTTAI--------VREPIGVCALITPWNWPIYQITAKVGPAL 172 Query: 222 ATGNSFILKPSERDPTPSLMLADLFKRAGLPDGVFNVVQGDKVAVDALLQ-HPDVQAISF 280 A G + +LKPSE P +L+ A++ AG+P GVFN+V G V A L HP V IS Sbjct: 173 AAGCTVVLKPSELSPLSALLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISI 232 Query: 281 VGSTPIAEYIYSEATKRAGTFPLRAQALGGAKNHLVVMPDANLEQAVDALIGAAYGSAGE 340 GST + A A T AQ LGG +L V+PDA+LE+A+ + AA + G+ Sbjct: 233 TGSTRAGVLVAQAA---AVTVKRVAQELGGKSPNL-VLPDADLERAIPPGVAAALRNMGQ 288 Query: 341 RCMAISVAVAVGSVADKLVEALIPRVKALKIRNGMEADAEMGPLVTATHKAKVEGYIDDG 400 C A + + + ++ E + + +K+ + +A GPL +V+ I+ G Sbjct: 289 SCSAPTRLIVPRAALARVHELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAG 348 Query: 401 VKAGAKLLVDGRGITVPGHENGFFVGGTLFDNVTLDMKIYKEEIFGPVLCVVRVPDFAEA 460 ++ GA+LL G G G G++ T+F V DM I +EEIFGPVL ++ EA Sbjct: 349 LQDGARLLAGGPG-RPDGLAQGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEA 407 Query: 461 VRLINAHEYGNGVSLFTADGNTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGD 520 + + N YG G + D + + RIQ G V +N P P A FGG+K+S G+ Sbjct: 408 IAIANDTVYGLGAHVQGRDKDQVRAVAARIQSGQVHLNYPAWDPQA--PFGGYKQS--GN 463 Query: 521 THAYGEEGIKFYTRYKSIM 539 YG EG++ Y K+I+ Sbjct: 464 GREYGVEGMEEYMEVKAIL 482 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 207 bits (527), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 145/462 (31%), Positives = 231/462 (50%), Gaps = 16/462 (3%) Query: 62 QHYINGRIAPSESGRQQDVFNPATGEVSRQVVLASTAEVNAAVASASAAAPGWAETAPLK 121 Q ++NG+ ++ G + V NPA GE+ V L AE A+ +A+AA P W + + Sbjct: 13 QAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKE 72 Query: 122 RARVLFKFKELIEQHHDDLAAAITREHGKVFSDAKGEVTRGLEIVEFACGVPQLLKTQFT 181 R+ +L ++ EL+ + DDLA +T E GK ++A+GE+ +E+ + K + Sbjct: 73 RSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEW---FAEEGKRTYG 129 Query: 182 DNIGGGIDNWN---LRQPLGVTAGITPFNFPVMVPLWMAPMAIATGNSFILKPSERDPTP 238 D I + +++ +GV A ITP+NFP + A A+A G +LKP+E P Sbjct: 130 DTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFS 189 Query: 239 SLMLADLFKRAGLPDGVFNVVQGDKVAVDA-LLQHPDVQAISFVGSTPIAEYIYSEATKR 297 +L LA L +RAG+P GVF+VV G + + +P V+ ISF GST + + + ++ Sbjct: 190 ALALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASS 249 Query: 298 AGTFPLRAQALGGAKNHLVVMPDANLEQAVDALIGAAYGSAGERCMAISVAVAVGSVADK 357 L LGG +V DA+L+ AV+ I + Y +AG+ C+ + V D Sbjct: 250 IKKLSLE---LGG-NAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDA 305 Query: 358 LVEALIPRVKALKIRNGMEADAEMGPLVTATHKAKVEGYIDDGVKAGAKLLVDGRGITVP 417 L+ VK K+ G E GPL+ KVE ++ D V GA++L+ G+ + Sbjct: 306 FAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHAL- 364 Query: 418 GHENGFFVGGTLFDNVTLDMKIYKEEIFGPVLCVVRVPDFAEAVRLINAHEYGNGVSLFT 477 GH F T+ +VT M++ +EE FGP+ + R E + L N E+G ++ Sbjct: 365 GHS---FFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYS 421 Query: 478 ADGNTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFG 519 D + ++ GMVGIN + + FGG K+S G Sbjct: 422 RDIGRIWRVAEGLESGMVGINTGL-ISNEVAPFGGVKQSGLG 462 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,674,500 Number of extensions: 70594 Number of successful extensions: 300 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 247 Number of HSP's successfully gapped: 20 Length of query: 558 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 468 Effective length of database: 1,193,720 Effective search space: 558660960 Effective search space used: 558660960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)