BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0072.p (251 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313914|ref|YP_003778006.1| oxidoreductase [Herbaspirillum ... 187 9e-49 gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogena... 105 3e-24 gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogena... 96 2e-21 gi|300311727|ref|YP_003775819.1| dehydrogenase [Herbaspirillum s... 89 2e-19 gi|300311620|ref|YP_003775712.1| short chain dehydrogenase/reduc... 77 1e-15 >gi|300313914|ref|YP_003778006.1| oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 187 bits (474), Expect = 9e-49, Method: Compositional matrix adjust. Identities = 104/229 (45%), Positives = 144/229 (62%), Gaps = 2/229 (0%) Query: 1 MKTTGNTILITGSTAGIGLGLALRFHQAGNKVIIAGRRKALLQQITTDHPGIEAIELDVA 60 M+ T NTI ITG +GIG GLA FH+ GN VII GRR+ALL ++ +PG++AI DVA Sbjct: 1 MQLTDNTIFITGGASGIGRGLAEAFHKLGNTVIIGGRRQALLDEVVAANPGMQAIAFDVA 60 Query: 61 DAKSIVQASEFVAERYPDLNVVINNAGIMLSESVLTPDSLKGAEETVTTNLLGTIRMTYA 120 D SI A+ + RYP LNV++NNAGIM + + A + TNLLG IR+T A Sbjct: 61 DPASIDAAAATLKARYPGLNVLVNNAGIMPFDDASGHIDDEVARRILDTNLLGPIRLTSA 120 Query: 121 FLPQLARKNDSVIINVSSALAFVPFPIAMTYSATKAAVHSFTEGLRVQLADTPVRVIELA 180 + L ++ + I++ +S LA+VP + YSA+KAA+HS+ R L +T VRV E+A Sbjct: 121 LIDLLKQQPRATILHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRETSVRVQEIA 180 Query: 181 PPGVRTTLFGQESDEQAMPLEEFLDEALSLLHSEPAPKEVIVERAKFLR 229 PP V T L + D +AMPL+ F+ E ++ L SE +E+IV+ K LR Sbjct: 181 PPWVDTDLVKKSGDPRAMPLDAFITETMARLASE--DEEIIVDAVKPLR 227 >gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 105 bits (263), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 65/178 (36%), Positives = 94/178 (52%), Gaps = 6/178 (3%) Query: 8 ILITGSTAGIGLGLALRFHQAGNKVIIAGRRKA----LLQQITTDHPGIEAIELDVADAK 63 +LITG+TAG G +A +F G+KVI AGRRK L++++ D + + LDV Sbjct: 3 VLITGATAGFGAAMARKFAGNGHKVIAAGRRKERLDDLVKELGADK--VLPLVLDVTSKS 60 Query: 64 SIVQASEFVAERYPDLNVVINNAGIMLSESVLTPDSLKGAEETVTTNLLGTIRMTYAFLP 123 SI + + +++V+INNAG+ L +L E + TN+ G + +T+A LP Sbjct: 61 SIETGLASLTGAWKEVDVLINNAGLALGLGPAHEVALSDWETMIDTNVKGLVTITHALLP 120 Query: 124 QLARKNDSVIINVSSALAFVPFPIAMTYSATKAAVHSFTEGLRVQLADTPVRVIELAP 181 + ++ IIN+ S P+P Y ATKA V FT LR L T VR LAP Sbjct: 121 DMVKRGRGTIINLGSVAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLAP 178 >gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 96.3 bits (238), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 69/190 (36%), Positives = 101/190 (53%), Gaps = 11/190 (5%) Query: 6 NTILITGSTAGIGLGLALRFHQAGNKVIIAGR--RKALLQQITTDHPGIEAIELDVADAK 63 TI ITG ++G GL +A F Q KVI R R LL P + + LDVADA Sbjct: 2 KTIFITGCSSGFGLEIARYFLQRDWKVIATMRTPRHDLL----PASPHLRILPLDVADAH 57 Query: 64 SIVQASEFVAERYPDLNVVINNAGIMLSESVLTPDSLKGAEETVTTNLLGTIRMTYAFLP 123 SI A E ++V++NNAGI + L + E TN LG+I MT AF+P Sbjct: 58 SIQAAVEAAG----PVDVLVNNAGIG-NLGALEAQPMAQVRELFETNTLGSIAMTQAFIP 112 Query: 124 QLARKNDSVIINVSSALAFVPFPIAMTYSATKAAVHSFTEGLRVQLADTPVRVIELAPPG 183 Q+ + ++INV+S++ P+ Y+A+KAA+++FTE L ++LA V+V + P Sbjct: 113 QMRARGQGLVINVTSSVTLRALPLLAVYTASKAAINAFTESLALELAPLGVQVRLVLPGR 172 Query: 184 VRTTLFGQES 193 T F + + Sbjct: 173 SPQTRFAENA 182 >gi|300311727|ref|YP_003775819.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 89.4 bits (220), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 72/211 (34%), Positives = 100/211 (47%), Gaps = 16/211 (7%) Query: 6 NTILITGSTAGIGLGLALRFHQAGNKVIIAGRRKALLQQITTDHPGIEAIELDVADAKSI 65 NT+LITG ++G GL A F G KVI R +D+ I + LDV+ SI Sbjct: 2 NTVLITGCSSGYGLETARHFLANGWKVIATMRSPRFDLLSPSDNLCI--LPLDVSQPDSI 59 Query: 66 VQASEFVAERYPDLNVVINNAGIMLSESV-LTPDSLKGAEETVTTNLLGTIRMTYAFLPQ 124 QA E ++V++NNAGI L + TP + E TN G + M A +PQ Sbjct: 60 KQALEAAGP----IDVLVNNAGIGLFGAFEATP--MSTVREIFETNTFGVMAMCQAVIPQ 113 Query: 125 LARKNDSVIINVSSALAFVPFPIAMTYSATKAAVHSFTEGLRVQLADTPVRVIELAPPGV 184 + D VIINV+S+ FP+ Y+A+K A+ FT L +L VRV +L PG Sbjct: 114 FRERGDGVIINVTSSATLAAFPLVAAYTASKTAIEGFTASLAHELKAVGVRV-KLVEPGY 172 Query: 185 RTTLFGQESDEQAM------PLEEFLDEALS 209 + + +Q M P E F E + Sbjct: 173 GPSTSFATNGQQRMQGLITEPYESFAHEVFA 203 >gi|300311620|ref|YP_003775712.1| short chain dehydrogenase/reductase [Herbaspirillum seropedicae SmR1] Length = 339 Score = 77.4 bits (189), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 65/197 (32%), Positives = 99/197 (50%), Gaps = 13/197 (6%) Query: 6 NTILITGSTAGIGLGLALRFHQAGNKVIIAGRRKALLQQITT--DHPGIEAIEL--DVAD 61 TI+ITG+++GIGL A ++++ R L+++ D G + L DVAD Sbjct: 11 QTIVITGASSGIGLATARLAAARQARLVLVARDAQALERVAAELDPSGRRVMHLAADVAD 70 Query: 62 AKSIVQASEFVAERYPDLNVVINNAGIML---SESVLTPDSLKGAEETVTTNLLGTIRMT 118 ++ + ER+ ++ INNAGI + E V SL+ TN G + + Sbjct: 71 LVALQAVAAQAVERFGGIDTWINNAGISIFGRHEEV----SLEDHRRLFETNYWGVVNGS 126 Query: 119 YAFLPQL-ARKNDSVIINVSSALAFVPFPIAMTYSATKAAVHSFTEGLRVQLADTPVRV- 176 A LP L A +V+IN+ S L+ V P+ YSA+K AV FT+ LRV+LA+ RV Sbjct: 127 LAALPHLKANPEGAVLINLGSELSDVAVPLQGAYSASKHAVKGFTDSLRVELAEQGARVS 186 Query: 177 IELAPPGVRTTLFGQES 193 + L P T F + + Sbjct: 187 VTLVKPAAIDTPFTRHA 203 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 731,535 Number of extensions: 28289 Number of successful extensions: 225 Number of sequences better than 1.0e-08: 34 Number of HSP's gapped: 192 Number of HSP's successfully gapped: 34 Length of query: 251 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 168 Effective length of database: 1,226,865 Effective search space: 206113320 Effective search space used: 206113320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)