BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0062.p (143 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311630|ref|YP_003775722.1| transmembrane protein [Herbaspi... 128 2e-31 >gi|300311630|ref|YP_003775722.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 144 Score = 128 bits (321), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 78/139 (56%), Positives = 100/139 (71%) Query: 2 INLVDFTPGLSLAGGLMIGGAIALLILFNGRIAGISGILGGLLGLPEKDVAWRAAFLIGM 61 ++ FTP +LAGG +IG A A+LIL NGR+AG+SGILGGLL D AWRA FL+G+ Sbjct: 3 VDWTAFTPVTALAGGALIGLAAAILILANGRVAGVSGILGGLLQPRAGDRAWRAMFLLGL 62 Query: 62 MLAPVIAIGGGVGIDIEIQSGWGEILTAGFLVGIGTRYAGGCTSGHGVCGISRGSLRSVV 121 + AP++ G I I +G G+I+ AG LVG+GTRY GCTSGHGVCG+SR SLRS V Sbjct: 63 LAAPLLMRLGASLPPIHIDAGLGQIVLAGLLVGLGTRYGAGCTSGHGVCGLSRFSLRSAV 122 Query: 122 ATLTFMAAGFATVFVKLHL 140 AT+ FM AGF +V++ H+ Sbjct: 123 ATVCFMMAGFVSVYLLRHV 141 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.147 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 291,253 Number of extensions: 8252 Number of successful extensions: 54 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 54 Number of HSP's successfully gapped: 1 Length of query: 143 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 66 Effective length of database: 1,255,275 Effective search space: 82848150 Effective search space used: 82848150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 126 (53.1 bits)