BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0053.p (282 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 194 6e-51 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 137 9e-34 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 132 2e-32 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 129 3e-31 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 128 4e-31 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 194 bits (494), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 101/250 (40%), Positives = 148/250 (59%) Query: 30 LEQFSLTGKTVLVTGAARDIGLEIARAMAGSGAHVILGGRNPGTLASAVQSLTAQGLSAG 89 L+ FSL GK L+TG++ IGL +AR MA +GA ++L GR+ G LA+ L A+G + Sbjct: 12 LQGFSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLRAEGHTIH 71 Query: 90 ALAFDVTDRAAVKDAFARIAKEHGGLDVLVNNAGVRNRKPLLEFSDDDIVAMLETNLVAG 149 +FD T VK A +I E G + +LVNNAG++ R PL EF + D ++ TNL + Sbjct: 72 ESSFDATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLISTNLNSV 131 Query: 150 YTLSREAARLMVPNKRGRLITVTSIAGQIARSGDAVYTGAKAGLTGMMRALAAEYGPLGI 209 + ++ AR M+P G++I + S+ ++ R G A YT K + + +A ++G GI Sbjct: 132 FFAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGI 191 Query: 210 TSNAIAPGGVATEANAASIADPKINAHFATRTPLGRWAQPAEIAGAAVFLASNAASYVNG 269 N + PG TE N A + D K RTP RW ++ GAAVFLAS+A+ +VNG Sbjct: 192 QVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNG 251 Query: 270 HVLYVDGGMS 279 H+LYVDGG++ Sbjct: 252 HILYVDGGVT 261 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 137 bits (345), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 95/246 (38%), Positives = 132/246 (53%), Gaps = 3/246 (1%) Query: 34 SLTGKTVLVTGAARDIGLEIARAMAGSGAHVILGGRNPGTLASAVQSLTAQGLSAGALA- 92 +L G+ LVTGA+R IG IA +A GA VI + A+ + L A G AG A Sbjct: 5 NLQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGRGAV 64 Query: 93 FDVTDRAAVKDAFARIAKEHGGLDVLVNNAGVRNRKPLLEFSDDDIVAMLETNLVAGYTL 152 +V D A ++ K+ G L +LVNNAG+ + + D++ +++ TNL A L Sbjct: 65 LNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRL 124 Query: 153 SREAARLMVPNKRGRLITVTSIAGQIARSGDAVYTGAKAGLTGMMRALAAEYGPLGITSN 212 SR R M+ + GR+I +TS+ G+ G Y AKAG+ GM RALA E G IT N Sbjct: 125 SRAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVN 184 Query: 213 AIAPGGVATEANAASIADPKINAHFATRTPLGRWAQPAEIAGAAVFLASNAASYVNGHVL 272 +APG + T+ A + + +A + PLGR EIA A FLAS A+Y+ G L Sbjct: 185 CVAPGFIDTDMTRA--LNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTL 242 Query: 273 YVDGGM 278 V+GGM Sbjct: 243 NVNGGM 248 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 132 bits (333), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 81/241 (33%), Positives = 128/241 (53%), Gaps = 1/241 (0%) Query: 38 KTVLVTGAARDIGLEIARAMAGSGAHVILGGRNPGTLASAVQSLTAQGLSAGALAFDVTD 97 + LVTG A IG I++ +A G V++ N + +L A G A L D+ Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGS 68 Query: 98 RAAVKDAFARIAKEHGGLDVLVNNAGVRNRKPLLEFSDDDIVAMLETNLVAGYTLSREAA 157 ++ AFA I K++G DVLVNNAGV L++ ++ + + N+ + AA Sbjct: 69 PESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHAA 128 Query: 158 RLMVPNKRGRLITVTSIAGQIARSGDAVYTGAKAGLTGMMRALAAEYGPLGITSNAIAPG 217 RLMV + GR++ ++SI+G A +G Y +KA + G+ R +A E GIT N++APG Sbjct: 129 RLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPG 188 Query: 218 GVATEANAASIADPKINAHFATRTPLGRWAQPAEIAGAAVFLASNAASYVNGHVLYVDGG 277 V T A ++ +++ P+ R+ EIA A +LA+ ++Y+ GHV+ VDGG Sbjct: 189 PVDTPMTQAMHSEQTRESYYRL-VPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDGG 247 Query: 278 M 278 Sbjct: 248 F 248 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 129 bits (324), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 92/252 (36%), Positives = 133/252 (52%), Gaps = 12/252 (4%) Query: 35 LTGKTVLVTGAA--RDIGLEIARAMAGSGAHVILGGRNPGTLASAVQSLTAQGLSAGALA 92 L K ++TG A +G AR MA GA +++ A A L AQ L L Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGAQHL---GLV 59 Query: 93 FDVTDRAAVKDAFARIAKEHGGLDVLVNNAGVRNRKPLLEFSDDDIVAMLETNLVAGYTL 152 DVTD+AA + A A + K+ G +D+LVNNAGV LLE + D +L+ +L + Sbjct: 60 ADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYM 119 Query: 153 SREAARLMVPNKRGRLITVTSIAGQIARSGDAV----YTGAKAGLTGMMRALAAEYGPLG 208 S+ M + G ++ ++S++ Q R G + Y+ AKAG+ G+ RA+A E+GP G Sbjct: 120 SQAVLPAMQAQQSGSIVCISSVSAQ--RGGGILGGPHYSAAKAGVLGLARAMAREFGPQG 177 Query: 209 ITSNAIAPGGVATEANAASIADPKINAHFATRTPLGRWAQPAEIAGAAVFLASNAASYVN 268 I N I PG ++T+ N ++ K A A PLGR P ++ G VFLAS+ + Y Sbjct: 178 IRVNCITPGLISTDINKDILSAEK-KAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCT 236 Query: 269 GHVLYVDGGMSI 280 G L V+GGM I Sbjct: 237 GVTLDVNGGMLI 248 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 128 bits (322), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 97/248 (39%), Positives = 126/248 (50%), Gaps = 6/248 (2%) Query: 34 SLTGKTVLVTGAARDIGLEIARAMAGSGAHVILGGRNPGTLASAVQSLTAQGLSAGALAF 93 +L GK L+TGA+ IG A A GA +IL R L + AQG A +A Sbjct: 3 TLQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVVAG 62 Query: 94 DVTDRAAVKDAFARIAKEHGGLDVLVNNAG-VRNRKPLLEFSDDDIVAMLETNLVAGYTL 152 DV + A + A E GGLD+ +NNAG V KPL E S L NL + + Sbjct: 63 DVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFLG 122 Query: 153 SREAARLMVPNKRGRLITVTSIAGQIA-RSGDAVYTGAKAGLTGMMRALAAEYGPLGITS 211 +R M+ G ++ +S G A G A Y AKAGL G+++ +AA+Y GI + Sbjct: 123 ARCQIPAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIRA 182 Query: 212 NAIAPGGVATEANAASIADPKINAHFATRTPLGRWAQPAEIAGAAVFLASNAASYVNGHV 271 NA+ PGGV T+ DP+ A + R AQP EIA AA+FLAS AS+V G Sbjct: 183 NALLPGGVDTDMG----GDPQQKQWAAGLHAMKRIAQPEEIAAAALFLASPMASFVTGTA 238 Query: 272 LYVDGGMS 279 LY DGG S Sbjct: 239 LYADGGNS 246 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 884,706 Number of extensions: 37732 Number of successful extensions: 323 Number of sequences better than 1.0e-08: 51 Number of HSP's gapped: 234 Number of HSP's successfully gapped: 51 Length of query: 282 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 198 Effective length of database: 1,222,130 Effective search space: 241981740 Effective search space used: 241981740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)