BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0051.p (354 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 366 e-102 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 248 5e-67 gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogena... 120 2e-28 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 115 4e-27 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 110 2e-25 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 366 bits (940), Expect = e-102, Method: Compositional matrix adjust. Identities = 176/221 (79%), Positives = 195/221 (88%), Gaps = 1/221 (0%) Query: 135 VFINNYGAGGGVXDYGGV-AAGGQARFIKTDVSRSADTAAMLAAALDAFGGVHIVVNNAG 193 + + + G GG+ + AAGG+A F K DVS+ AD +LA AL+ FG + IVVNNAG Sbjct: 32 IVVADIGEAGGLRVVEEIKAAGGRAVFAKADVSKRADMDQLLATALEHFGKLDIVVNNAG 91 Query: 194 TTHRNRPMLEIEEDEFERVYAINVKSIFLSAKTFVPYFRKVGGGAFINIASTAGIRPRPG 253 TTHRNRPMLE+EEDEF+RVYA+NVKSIFLSAKTFVPYFR+VGGGAFINIASTAGIRPRPG Sbjct: 92 TTHRNRPMLEVEEDEFDRVYAVNVKSIFLSAKTFVPYFRQVGGGAFINIASTAGIRPRPG 151 Query: 254 LTWYNGSKGAVITTSKSMAAELGPDNIRVNCVNPVISATGLLTEFMGVPDTPENRKKFTA 313 LTWYNGSKGAVITTSKSMAAELGPD IRVNCVNPVISATGLL+EFMGVPDTPENRKKF A Sbjct: 152 LTWYNGSKGAVITTSKSMAAELGPDKIRVNCVNPVISATGLLSEFMGVPDTPENRKKFVA 211 Query: 314 TIPLGRFSTPDDIANACLYLGSDEASFITGACLEVDGGRCV 354 TIP+GRFSTP+DIANACLYLGSDEA F+TG C+EVDGGRCV Sbjct: 212 TIPMGRFSTPEDIANACLYLGSDEAEFVTGVCIEVDGGRCV 252 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 248 bits (633), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 125/160 (78%), Positives = 134/160 (83%), Gaps = 1/160 (0%) Query: 1 LERVGAAFGKLRVGSAAMDLDCGPLIRKTQQERVAGFLRDAADSRIPVVAQGRIADGVPA 60 LER+ F +L+VGS+ MDLDCGPLIRKTQQERVAGFL DA I + AQG+IA GVP Sbjct: 297 LERLSGLFSQLQVGSSQMDLDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGVPT 356 Query: 61 GGFYQAPTLLRDVPIQHRLAQEEVFGPVLAAMPFEDEADAIRLANGTDFGLVASVWTRDG 120 GGFYQAP LLRDVP +HRLAQEEVFGPVLAAMPFEDEA AI LANGTD+GLVASVWTRDG Sbjct: 357 GGFYQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDG 416 Query: 121 GRQFRLARAIRSGQVFINNYGAGGGVX-DYGGVAAGGQAR 159 GRQ RLARAIRSGQVFINNYGAGGGV +GGV A G R Sbjct: 417 GRQMRLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGR 456 >gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 266 Score = 120 bits (300), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 82/251 (32%), Positives = 127/251 (50%), Gaps = 25/251 (9%) Query: 113 ASVWTRDGGRQFRLARAIRSGQVFINNYGAGGGVXDYGGVAAGGQARFIKTDVSRSADTA 172 A ++ R+GGR + R+I + + + GGV + + DVSR+ + Sbjct: 29 AVLYAREGGRVLAVDRSIEAALQTQDIIRSEGGVCE-----------VLAADVSRNTEVK 77 Query: 173 AMLAAALDAFGGVHIVVNNAGTTHRNRPMLEIEEDEFERVYAINVKSIFLSAKTFVPYFR 232 A+ AA+D FG V ++ NN G P +E E+ + R+ A+N S+FL+ K +P Sbjct: 78 AIAEAAMDHFGRVDVLHNNVGIADVGGP-VETSEESWNRLVAVNQTSLFLTHKHVLPIME 136 Query: 233 KVGGGAFINIASTAGIR----PRPGLTWYNGSKGAVITTSKSMAAELGPDNIRVNCVNPV 288 K GA +NI+S A +R P G Y +K A++ +K++A + P IR NCV P Sbjct: 137 KQRKGAIVNISSLAALRWIGFPYLG---YTATKAAILAFTKNVAMQYAPMGIRANCVLPG 193 Query: 289 ISATGLLTEFMGVP---DTPENRKKFTATIPLGRFSTPDDIANACLYLGSDEASFITGAC 345 + T ++ E + D + R K A P+G D+A+A L+L SD A +ITG Sbjct: 194 MMDTPMIREPLKASYGGDIEQMRAKRNAQCPMGFMGDAWDVAHASLFLASDHARYITGLD 253 Query: 346 LEVDGG---RC 353 + VDGG RC Sbjct: 254 MIVDGGLSLRC 264 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 115 bits (289), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 69/200 (34%), Positives = 108/200 (54%), Gaps = 6/200 (3%) Query: 155 GGQARFIKTDVSRSADTAAMLAAALDAFGGVHIVVNNAGTTHRNRPMLEIEEDEFERVYA 214 G ++ I DV ++ A +A A+ G + +++NNAG + P+ + ++ F+ + Sbjct: 55 GSKSITIGADVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIIN 114 Query: 215 INVKSIFLSAKTFVPYFRKVGGGAFINIASTAGIR-PRPGLTWYNGSKGAVITTSKSMAA 273 IN +S+ + +P R GGG IN+ S A PG Y SKG V T ++ MA Sbjct: 115 INARSVVAFTRAVIPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAK 174 Query: 274 ELGPDNIRVNCVNPVISATGLLTEFMGVPDTPENRKKFTATIPLGRFSTPDDIANACLYL 333 EL D IRVN V P + ++T F TPE + F TIP+GR PD+ + A LYL Sbjct: 175 ELLADKIRVNAVAPGV----IMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYL 230 Query: 334 GSDEAS-FITGACLEVDGGR 352 S++ S ++TG ++V+GG+ Sbjct: 231 ASEQMSGYVTGQIIDVNGGQ 250 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 110 bits (274), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 64/205 (31%), Positives = 109/205 (53%), Gaps = 13/205 (6%) Query: 155 GGQARFIKTDVSRSADTAAMLAAALDAFGGVHIVVNNAGTTHRNRPMLEIEEDEFERVYA 214 G Q + DV+ A A AA L FG + I+VNNAG T + +LEI +++R+ Sbjct: 52 GAQHLGLVADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAK-LLEITGKDYDRILD 110 Query: 215 INVKSIFLSAKTFVPYFRKVGGGAFINIASTA-----GIRPRPGLTWYNGSKGAVITTSK 269 ++++ ++ +P + G+ + I+S + GI P Y+ +K V+ ++ Sbjct: 111 VSLRGTLYMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGP---HYSAAKAGVLGLAR 167 Query: 270 SMAAELGPDNIRVNCVNPVISATGLLTEFMGVPDTPENRKKFTATIPLGRFSTPDDIANA 329 +MA E GP IRVNC+ P + +T + + + + E + + TIPLGR PDD+ Sbjct: 168 AMAREFGPQGIRVNCITPGLISTDINKDIL----SAEKKAEIAQTIPLGRLGGPDDVGGV 223 Query: 330 CLYLGSDEASFITGACLEVDGGRCV 354 C++L SD + + TG L+V+GG + Sbjct: 224 CVFLASDLSQYCTGVTLDVNGGMLI 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,051,530 Number of extensions: 43336 Number of successful extensions: 235 Number of sequences better than 1.0e-08: 55 Number of HSP's gapped: 186 Number of HSP's successfully gapped: 55 Length of query: 354 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 268 Effective length of database: 1,212,660 Effective search space: 324992880 Effective search space used: 324992880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)