BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0050.p (438 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 744 0.0 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 524 e-150 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 424 e-119 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 392 e-110 gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 365 e-102 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 744 bits (1921), Expect = 0.0, Method: Compositional matrix adjust. Identities = 362/421 (85%), Positives = 397/421 (94%) Query: 18 SFEEATFSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSETVYGLGAGIFFIG 77 SFE+AT+SKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFS+TVYGLGAGIFF+G Sbjct: 15 SFEDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLG 74 Query: 78 YFIFEVPSNIILHKVGARLWIARIMITWGIISGAMIFVTTPEMFYVMRFLLGVAEAGFFP 137 YF+FEVPSN+ILHKVGARLWIARIMITWGIISGAM +VTTPEMFY+MRFLLGVAEAGFFP Sbjct: 75 YFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFP 134 Query: 138 GVILYLTYWYPSHRRGKITALFMTGIAISGVIGGPLSGWIMHAFPGVHGLTGWQWMFIIE 197 GVILYLTYWYP+ RRGKITALFMTGIA+SGVIGGPLSGWIMHA PGV G TGWQWMFI+E Sbjct: 135 GVILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILE 194 Query: 198 AIPSLVLGVVVIFYLKDRIRDADWLSEDEKRLLESQIAQEQSEKAEVSLGQMFADGKVWL 257 AIPSL+LGV+VI Y+KDRIRDADWLSE+EKRLLE+ I+ E+S+K +SLG MFA+G+VWL Sbjct: 195 AIPSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQKEHLSLGAMFANGRVWL 254 Query: 258 SAIIYFCFVMGLYGVSFWLPTIIKTTGVTDPLNIGLLTAIPYAVAAVAMIMIGRSADQHR 317 A+IYFCFVMGLYGVSFWLPTIIK TGV+DPLN+GLLTAIPYA AA AM++IGRSAD R Sbjct: 255 GALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARR 314 Query: 318 ERRWHVAIPAFLGCVGLLFSTVYDHNTLLAMASLTLAAVGILTTLPLFWSLPTAFLSGAA 377 ERRWHVAIPAF+GC+GLL ST Y HNT LAMASLTLA+VGILTTLPLFWSLPTAFLSG A Sbjct: 315 ERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTA 374 Query: 378 AAAGIALINSLGNLAGFVSPYLVGWLKDATQSTNTGMYVLAASLLLGGLLTLAVPAKLVN 437 AAAGIALINSLGNLAGFVSP+LVGWLKDATQSTN GMYVLAASL++GGLLTL +PAKLVN Sbjct: 375 AAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLTLTLPAKLVN 434 Query: 438 K 438 + Sbjct: 435 R 435 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 524 bits (1350), Expect = e-150, Method: Compositional matrix adjust. Identities = 255/428 (59%), Positives = 335/428 (78%), Gaps = 3/428 (0%) Query: 14 GTDVS--FEEATFSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSETVYGLGA 71 G DV+ ++ + KV WR++P L LCYV +YLDRVNVGFAKLQM QDL FSETV+GLGA Sbjct: 10 GLDVNDKSKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGA 69 Query: 72 GIFFIGYFIFEVPSNIILHKVGARLWIARIMITWGIISGAMIFVTTPEMFYVMRFLLGVA 131 G+FFIGYF+FEVPSNI++HKVGAR+WIARIMITWGI+S A +FV FY++RFLLG+A Sbjct: 70 GLFFIGYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLA 129 Query: 132 EAGFFPGVILYLTYWYPSHRRGKITALFMTGIAISGVIGGPLSGWIMHAFPGVHGLTGWQ 191 EAGF+PG+ILYLTYW+PSHRR K+ A+FM+GI ++G++G PLSGWIM AF GL GWQ Sbjct: 130 EAGFYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQ 189 Query: 192 WMFIIEAIPSLVLGVVVIFYLKDRIRDADWLSEDEKRLLESQI-AQEQSEKAEVSLGQMF 250 WMF+IEAIP++++GV + YL + ++ A WL+++EK L++ I + ++++ G + Sbjct: 190 WMFLIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAIV 249 Query: 251 ADGKVWLSAIIYFCFVMGLYGVSFWLPTIIKTTGVTDPLNIGLLTAIPYAVAAVAMIMIG 310 D +VWL +IYF FVMG YG++ W+PT++K TGV L IGLL+AIP+ A +AM +IG Sbjct: 250 KDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIG 309 Query: 311 RSADQHRERRWHVAIPAFLGCVGLLFSTVYDHNTLLAMASLTLAAVGILTTLPLFWSLPT 370 RSAD+ RERRWH+ IPA +G VG + + ++ NT +++ASL+LAA G+LT PLFWSLPT Sbjct: 310 RSADRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPT 369 Query: 371 AFLSGAAAAAGIALINSLGNLAGFVSPYLVGWLKDATQSTNTGMYVLAASLLLGGLLTLA 430 AFLSGAAAA GIA INS+GNLAGFVSPYL+G+LKD T + TGMY+LA L++G + TLA Sbjct: 370 AFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGSIATLA 429 Query: 431 VPAKLVNK 438 +VNK Sbjct: 430 TKPGMVNK 437 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 424 bits (1089), Expect = e-119, Method: Compositional matrix adjust. Identities = 219/410 (53%), Positives = 290/410 (70%), Gaps = 1/410 (0%) Query: 21 EATFSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSETVYGLGAGIFFIGYFI 80 E T+ K+ WRL+P L +V +++DRVNVGFAKLQMLQDL+FSE VYGLGAGIFFIGYFI Sbjct: 22 EVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFI 81 Query: 81 FEVPSNIILHKVGARLWIARIMITWGIISGAMIFVTTPEMFYVMRFLLGVAEAGFFPGVI 140 FEVPSN++L K+GAR +ARI I WG+ S AM +VTTP FY++RFLLG+ EAGFFPGV+ Sbjct: 82 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVV 141 Query: 141 LYLTYWYPSHRRGKITALFMTGIAISGVIGGPLSGWIMHAFPGVHGLTGWQWMFIIEAIP 200 LYLTYW+P+ RR ++ LFMT AI+G +GGP++G IM GV L WQW+FI+E IP Sbjct: 142 LYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIP 201 Query: 201 SLVLGVVVIFYLKDRIRDADWLSEDEKRLLESQIAQEQSEKAE-VSLGQMFADGKVWLSA 259 S++ G V+ +L +R +A WLSE EK + +A E S + S + +VW+ A Sbjct: 202 SILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENSAPGKHFSFKDACTNYRVWICA 261 Query: 260 IIYFCFVMGLYGVSFWLPTIIKTTGVTDPLNIGLLTAIPYAVAAVAMIMIGRSADQHRER 319 +YFC V G ++FW P+IIK GV L IGL++AIP+ +AMI G +D+ ER Sbjct: 262 AVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGER 321 Query: 320 RWHVAIPAFLGCVGLLFSTVYDHNTLLAMASLTLAAVGILTTLPLFWSLPTAFLSGAAAA 379 R H AI + C+GL+ + + N LA+ SLTLAA+GIL P+FWS+P AFL+G AAA Sbjct: 322 RLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAA 381 Query: 380 AGIALINSLGNLAGFVSPYLVGWLKDATQSTNTGMYVLAASLLLGGLLTL 429 GIALINS+GNLAGFV+PY++G LK +T S ++G+Y +AA L+ L + Sbjct: 382 GGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVASFLVV 431 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 392 bits (1008), Expect = e-110, Method: Compositional matrix adjust. Identities = 207/426 (48%), Positives = 286/426 (67%), Gaps = 10/426 (2%) Query: 11 ASPGTDVSFEEATFSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSETVYGLG 70 AS G+ V F+ + KVTW++LP L L YV +YLDRVNVGFAKL ML DL SE YGLG Sbjct: 4 ASQGSAVQFD-GLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLG 62 Query: 71 AGIFFIGYFIFEVPSNIILHKVGARLWIARIMITWGIISGAMIF---------VTTPEMF 121 AGIFF+GYFIFEVPSN+ILH+VGA++WIARIMITWG+IS M F V+ +F Sbjct: 63 AGIFFLGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVF 122 Query: 122 YVMRFLLGVAEAGFFPGVILYLTYWYPSHRRGKITALFMTGIAISGVIGGPLSGWIMHAF 181 Y +RFLLG +EAGFFPGVILY+TYWYP+ ++ A+ + G S ++GGP+SG +M Sbjct: 123 YTLRFLLGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFM 182 Query: 182 PGVHGLTGWQWMFIIEAIPSLVLGVVVIFYLKDRIRDADWLSEDEKRLLESQIAQEQSEK 241 L GWQWMF+IEA+P+ +LG+V++F L + I A WL+ +EK L ++A E EK Sbjct: 183 GSGDSLAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKEK 242 Query: 242 AEVSLGQMFADGKVWLSAIIYFCFVMGLYGVSFWLPTIIKTTGVTDPLNIGLLTAIPYAV 301 V L ++A +W A++Y ++G YG++FW PTII+ GV L+IG+++AIPY + Sbjct: 243 VNVPLKSLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYIL 302 Query: 302 AAVAMIMIGRSADQHRERRWHVAIPAFLGCVGLLFSTVYDHNTLLAMASLTLAAVGILTT 361 A AM+ I R A++ + R + + + + L+ T++ HN + +A + +A G LT+ Sbjct: 303 GATAMVFITRHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLTS 362 Query: 362 LPLFWSLPTAFLSGAAAAAGIALINSLGNLAGFVSPYLVGWLKDATQSTNTGMYVLAASL 421 + LFW P A+ +GA AAAGIA+INS+GNL GFV PYL+G L A S G+ +L +L Sbjct: 363 IALFWGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMMLGGAL 422 Query: 422 LLGGLL 427 L G+L Sbjct: 423 FLAGVL 428 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 365 bits (937), Expect = e-102, Method: Compositional matrix adjust. Identities = 193/415 (46%), Positives = 274/415 (66%), Gaps = 6/415 (1%) Query: 20 EEATFSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSETVYGLGAGIFFIGYF 79 E+AT KV RL+P + LCYV SYLDR+NVGFA L M +DL + + +GLGAG+FFIGYF Sbjct: 20 EDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF 79 Query: 80 IFEVPSNIILHKVGARLWIARIMITWGIISGAMIFVTTPEMFYVMRFLLGVAEAGFFPGV 139 FE+PSN+ LH+ GAR+WI+RIMI+WG+IS A FV P+ F + RFLLG+AEAGF PG+ Sbjct: 80 FFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGI 139 Query: 140 ILYLTYWYPSHRRGKITALFMTGIAISGVIGGPLSGWIMHAFPGVHGLTGWQWMFIIEAI 199 LY T W+P RGK TA F+ GI ++ +IG PLSG +M G+ G GWQ + ++EA+ Sbjct: 140 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALME-LHGMWGFKGWQVLLLLEAL 198 Query: 200 PSLVLGVVVIFYLKDRIRDADWLSEDEKRLLESQIAQEQS---EKAEVSLGQMFADGKVW 256 P+++LGV+ +F L DR A WLS DEK+ LE++++ EQ+ + L F + +V+ Sbjct: 199 PAVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVF 258 Query: 257 LSAIIYFCFVMGLYGVSFWLPTIIKTTGVTDPLNIGLLTAIPYAVAAVAMIMIGRSADQH 316 A FC ++G + WLP II+ G+ +GL+ AIPY V AVAM + R A++ Sbjct: 259 ALAGANFCGIIGSLSIGLWLPQIIREFGLPSH-QVGLVAAIPYLVGAVAMTLWARLANRS 317 Query: 317 RERRWHVAIPAFLGCVGLLFSTVYDHNTLLAMASLTLAAVGILTTLPLFWSLPTAFLSGA 376 R + VA L + L S + H LL M ++T+A IL+ FW++P+ FL+G Sbjct: 318 ERRLFFVAGAIVLAALSLGISA-FLHTPLLKMLAITVAVASILSFQATFWAIPSGFLTGR 376 Query: 377 AAAAGIALINSLGNLAGFVSPYLVGWLKDATQSTNTGMYVLAASLLLGGLLTLAV 431 AAA G+ALI S+GNL GFV P ++G++++ATQ + +A +LLLG ++TLA+ Sbjct: 377 AAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAGALLLGAVITLAL 431 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,263,914 Number of extensions: 50139 Number of successful extensions: 311 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 279 Number of HSP's successfully gapped: 20 Length of query: 438 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 350 Effective length of database: 1,203,190 Effective search space: 421116500 Effective search space used: 421116500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)