BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0036.p (272 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydro... 270 1e-73 gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 79 4e-16 gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 70 3e-13 gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 66 3e-12 gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [H... 55 6e-09 >gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 270 bits (690), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 147/272 (54%), Positives = 184/272 (67%), Gaps = 5/272 (1%) Query: 1 VVEAIGADVTGFKVGDQVFYAGDITRPGSNSEFQLVDERIVGHAPASLSVEQAAALPLTA 60 VV A+G VT F GD+VFYAG ITRPG+NSE +VDERIVG P+SL AAA+PLTA Sbjct: 67 VVRAVGPAVTLFAPGDEVFYAGSITRPGANSELHVVDERIVGRKPSSLGHADAAAIPLTA 126 Query: 61 ITAWEALFERLRVPRKAEAASGKAPSILIIGGAGGVGSIAIQLAAKVAGLNVIATASRPE 120 ITAWE LF+R+ V AE G+ +LIIG AGGVGS+ QLA K+ L VI TASRPE Sbjct: 127 ITAWELLFDRVAV---AEGG-GQGQHLLIIGAAGGVGSMLTQLARKLTQLTVIGTASRPE 182 Query: 121 SEKWVRELGAQQVINHFGDIPAQLKGIGFEQVDHVLILNDTDKHFPAAAQVIAPQGTIAT 180 + WV ELGA VI+H + QL+ +G +V V+ L TD+H+ V+APQG +A Sbjct: 183 TAHWVGELGAHHVIDHSQPLKPQLEKLGIPEVGIVISLTHTDQHYAQIIDVLAPQGQLA- 241 Query: 181 IVENSAPLDVSLLKAKSAGFVWEFMFTRSMFQTPDMGEQGKLLNEIAALIDRGVLRTTLS 240 ++++ A LD LK KS WE MFTRSMFQTPDM Q +LL ++ L+D+GVLRTT Sbjct: 242 LIDDPASLDAMPLKRKSISLHWELMFTRSMFQTPDMIRQHELLQRVSQLLDQGVLRTTTG 301 Query: 241 KVLSPINAANLRQAHADLEGGRVIGKIVLKDF 272 + I+AANLR+AH LE G+ GKIVL+ F Sbjct: 302 EHYGRIDAANLRRAHGLLESGKAKGKIVLEGF 333 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 79.0 bits (193), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 90/282 (31%), Positives = 134/282 (47%), Gaps = 39/282 (13%) Query: 1 VVEAIGADVTGFKVGDQVFYAGDITRP-GSNSEFQLVDERIVGHAPASLSVEQAAALPLT 59 +EA+GA V+GFKVGD+V YAG RP G+ +E +++ I+ P ++S + AAA+ L Sbjct: 67 TIEAVGAGVSGFKVGDRVAYAG---RPNGAYAEERVMPADILVKLPDAISFDTAAAMMLQ 123 Query: 60 AITAWEALFERLRVPRKAEAASGKAPSILIIGGAGGVGSIAIQLAAKVAGLNVIATASRP 119 +T L + V +IL AGG+G IA Q A+ G+N+I T S Sbjct: 124 GMTVQYLLRQTYAVQ--------PGDTILFHAAAGGIGLIACQW-ARALGVNLIGTVSSD 174 Query: 120 ESEKWVRELGAQQVINH----FGDIPAQLKGIGFEQVDHVLILNDTDKHFPAAAQVIAPQ 175 E + GA VIN+ F + +L G QV + I DT F + + P+ Sbjct: 175 EKAALALQHGAAHVINYKTENFVERVKELTGGRGVQVVYDSIGKDT---FLGSLDCLRPR 231 Query: 176 GTIATIVENS---APLDVSLLKAKSAGFVWEFMFTRSMFQTPDMGEQGKLLNEIAA---- 228 G + + S AP VS L ++ + F+ TR P +G E+ A Sbjct: 232 GMMVSFGNASGPVAPFGVSELASRGSLFL-----TR-----PSLGNYTSTRAELDATAGD 281 Query: 229 LIDRGVLRTTLSKVLSPINA-ANLRQAHADLEGGRVIGKIVL 269 L D VL +S ++ A ++ QAHADLEG + G +L Sbjct: 282 LFDM-VLGNKISIEINQRYALKDVAQAHADLEGRKTTGSTIL 322 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 69.7 bits (169), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 78/266 (29%), Positives = 122/266 (45%), Gaps = 23/266 (8%) Query: 10 TGFKVGDQVFYAGDITRPGSNSEFQLVDERIVGHAPASLSVEQAAALPLTAITAWEALFE 69 +GFK+GD V + + G +EF P LS +AAALP T W +F+ Sbjct: 77 SGFKLGDLVCA---LVQGGGYAEFCAAPLAQCLPVPKGLSALEAAALPENYFTVWSNVFD 133 Query: 70 RLRVPRKAEAASGKAPSILIIGGAGGVGSIAIQLAAKVAGLNVIATASRPESEKWVRELG 129 R ++ SG+ ++L+ GG+ G+G AIQ+ A+ G V ATA E + ELG Sbjct: 134 RGQL-------SGEE-TLLVQGGSSGIGVTAIQI-ARALGHRVFATAGSAEKCRACEELG 184 Query: 130 AQQVINH-FGDIPAQLKGI-GFEQVDHVLILNDTDKHFPAAAQVIAPQGTIATIVE---N 184 A++ IN+ D A +K + G VD +L + D + P + +A G + I + Sbjct: 185 AERAINYKTEDFEAVVKELTGGRGVDVILDMVGGD-YLPREIKALADDGRLVFIAQLGGA 243 Query: 185 SAPLDVSLLKAKSAGFVWEFMFTRSMFQTPDMGEQGKLLNEIAALIDRGVLRTTLSKVLS 244 LD+ + + + R + + KL + LI+ G +R + Sbjct: 244 KGQLDMGQVMRRRLTITGSTLRPRPVAFKAAIA--AKLREHVWPLIEAGKIRPVIHHRFP 301 Query: 245 PINAANLRQAHADLEGGRVIGKIVLK 270 AA QAHA +EG IGKI+L+ Sbjct: 302 LEQAA---QAHAMMEGSTHIGKIMLE 324 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 66.2 bits (160), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 81/289 (28%), Positives = 124/289 (42%), Gaps = 51/289 (17%) Query: 1 VVEAIGADVTGFKVGDQV-----FYAGDITRPGSNSEFQLVDERIVGHAPASLSVEQAAA 55 VVEA+ DV F VGD+V F +G + +++ V + P +S QAAA Sbjct: 79 VVEAVADDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVAASEIALKPQRISHAQAAA 138 Query: 56 LPLTAITAWEALFE------RLRVPRKAEAASGKAPSILIIGGAGGVGSIAIQLAAKVAG 109 P++ +TAW+ + E P + E K ++++ G AGGVG A+QL AK G Sbjct: 139 APMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNGAAGGVGHFALQL-AKWKG 197 Query: 110 LNVIATASRPESEKWVRELGAQQVINH--------FGDIPAQLKGIGFEQVDHVL-ILND 160 VIA S E ++R+LGA + I++ D+ + +G L L Sbjct: 198 ARVIAIGSG-GHEAFLRDLGADEFIDYTRSAAEDVLSDVDLVIDAVGGPASGRFLRTLKR 256 Query: 161 TDKHFPAAAQVIAPQGTIATIVENSAPLDVSLLKAKSAGFVWEFMFTRSMFQTPDMGEQG 220 FP I P G + E +A L V++ T + G Sbjct: 257 GGALFP-----IFPLGF--SGAEEAARLGVNV-------------------STTQVRSSG 290 Query: 221 KLLNEIAALIDRGVLRTTLSKVLSPINAANLRQAHADLEGGRVIGKIVL 269 L ++A L+D GV+R + + +A +AH G + GKIVL Sbjct: 291 AQLQQLAGLLDSGVVRVAIDSTFALQDAG---KAHERAAQGHIRGKIVL 336 >gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 55.1 bits (131), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 78/271 (28%), Positives = 131/271 (48%), Gaps = 36/271 (13%) Query: 1 VVEAIGADVTGFKVGDQVFYAGDITRPGSN-SEFQLVDERIVGHAPASLSVEQAAALPLT 59 V+ A+G V +VG+ V + G+ +E L E++V P LS++QAAAL Sbjct: 70 VITALGEGVHTLQVGEAVCVMPSMAGRGTTLAELVLPQEQVV-RKPDVLSMQQAAALLQP 128 Query: 60 AITAWEALFERLRVPRKAEAASGKAPSILIIGGAGGVGSIAIQLAAKVAGLNVIATASRP 119 A +AW AL + A A+G + I G+G VGS+ +QLAA + G V A+ R Sbjct: 129 ACSAWIALMQ------TAPIAAGM--KVFIHAGSGAVGSLMVQLAAHL-GAEVTAS-CRS 178 Query: 120 ESEKWVRELGAQQVINHFGDIPAQLKGIGFEQVDHVLILNDTDKHFPAAAQVIAPQGTIA 179 + ++V LGA +V+ + D +++ D V+ L H + +++ G + Sbjct: 179 SNVEYVAALGASRVLAYDRDELGRVR-----DQDVVIDLVGGAAH-DQSYRMLRRGGQLV 232 Query: 180 TIVENSAPLDVSLLKAKSAGFVWEFMFTRSMFQTPDMGEQGKLLNEIAALIDRGVLRTTL 239 +V +AP+ + G + R++ E +L ++AAL +GV R + Sbjct: 233 WLV--AAPI-------RERGEEFGVSVRRALIV-----EDQSILQQVAALAQQGVWRPAV 278 Query: 240 SKVLSPINAANLRQAHADLEGGRVI-GKIVL 269 S VL P+ + +AH +E G V G++VL Sbjct: 279 SGVL-PMQ--EIARAHQRIEAGEVTRGRLVL 306 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 814,160 Number of extensions: 31559 Number of successful extensions: 128 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 125 Number of HSP's successfully gapped: 5 Length of query: 272 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 188 Effective length of database: 1,222,130 Effective search space: 229760440 Effective search space used: 229760440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)