BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0023.p (94 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313094|ref|YP_003777186.1| hypothetical protein Hsero_3800... 90 3e-20 >gi|300313094|ref|YP_003777186.1| hypothetical protein Hsero_3800 [Herbaspirillum seropedicae SmR1] Length = 75 Score = 90.1 bits (222), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 41/71 (57%), Positives = 52/71 (73%) Query: 21 MTKLEEIVKRQKQGAKFVLSFQMLGMDVETFDTVAQTWLEHGGPGFEAAGVPFRKVIDGE 80 M KLE+IV++QK GA FV+S QML M FD +AQ W + GGPGF A VPFR V++ E Sbjct: 1 MAKLEDIVRKQKPGATFVISAQMLQMSPREFDALAQVWDDEGGPGFNVAAVPFRVVVEDE 60 Query: 81 FLIARLTVVKT 91 F I+R+TVV+T Sbjct: 61 FFISRVTVVRT 71 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 285,222 Number of extensions: 9942 Number of successful extensions: 34 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 34 Number of HSP's successfully gapped: 1 Length of query: 94 Length of database: 1,619,870 Length adjustment: 63 Effective length of query: 31 Effective length of database: 1,321,565 Effective search space: 40968515 Effective search space used: 40968515 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 123 (52.0 bits)