BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0028.0022.p
         (326 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311876|ref|YP_003775968.1| phenylacetyl-CoA-ligase [Herbas...   563   e-162
gi|300313420|ref|YP_003777512.1| phenylacetyl-CoA-ligase [Herbas...   112   4e-26

>gi|300311876|ref|YP_003775968.1| phenylacetyl-CoA-ligase
           [Herbaspirillum seropedicae SmR1]
          Length = 423

 Score =  563 bits (1452), Expect = e-162,   Method: Compositional matrix adjust.
 Identities = 266/326 (81%), Positives = 293/326 (89%)

Query: 1   VSPGPIFDPEGHSQDWWRFERPMRALGLRAGHIIQNCFSYHFTPAAFMAEGAAAKLGCAV 60
           VSPGPIFDPEGH  DWWRF  PM+ALGL+AGHI+QNCF+YHFTPAAFM EGAAA+LGCAV
Sbjct: 98  VSPGPIFDPEGHGADWWRFASPMKALGLQAGHILQNCFAYHFTPAAFMVEGAAARLGCAV 157

Query: 61  IPSGIGQTELQVQTMASLRPDAYVGTPSFLKIIIEKAQEMGVALDSLQRALVSAEALPPS 120
           IP+GIGQTELQV  MA+L+PDAYVGTPSFLKIIIEKAQEMG  + S+QRALVSAEALPPS
Sbjct: 158 IPAGIGQTELQVNAMAALKPDAYVGTPSFLKIIIEKAQEMGADISSVQRALVSAEALPPS 217

Query: 121 LRSWFHDNGVPNVLQLYASADIGNIAYESISEGKVNPGMVLDEDLILEIVRPGTGDPVRP 180
           LR+WFH++GVP VLQLYASADIGNIAYE++SEG V+PGMVLDE LILEIVRPGTGDPV  
Sbjct: 218 LRNWFHEHGVPQVLQLYASADIGNIAYETMSEGAVHPGMVLDEALILEIVRPGTGDPVAA 277

Query: 181 GEVGEVVVTSFNPDYPLIRFGTGDLTATLDGVSPCGRTNTRIKGWMGRADQTTKVRGMFV 240
           GEVGEVVVTSFNPDYPLIRFGTGDL+A + GVSPCGRTNTRIKGWMGRADQTTKVRGMFV
Sbjct: 278 GEVGEVVVTSFNPDYPLIRFGTGDLSAVVPGVSPCGRTNTRIKGWMGRADQTTKVRGMFV 337

Query: 241 HPSQVAEITRRHGLILKARLVVSGEMANDSMTLHCETQDMESAVAQAAAIADSVRDVTKL 300
           HPSQVA++ RRH  +L+ARLVVSGEMAND MTLHCE  D E+      A+A S+R+VTKL
Sbjct: 338 HPSQVADVLRRHADVLRARLVVSGEMANDVMTLHCEVGDPENPAIDVGALAGSLREVTKL 397

Query: 301 RGDVLFAAPGSLPNDGKVIEDARKYE 326
           RG+VL  APGSLPNDGKVIEDARKYE
Sbjct: 398 RGEVLLVAPGSLPNDGKVIEDARKYE 423


>gi|300313420|ref|YP_003777512.1| phenylacetyl-CoA-ligase
           [Herbaspirillum seropedicae SmR1]
          Length = 446

 Score =  112 bits (280), Expect = 4e-26,   Method: Compositional matrix adjust.
 Identities = 85/265 (32%), Positives = 133/265 (50%), Gaps = 11/265 (4%)

Query: 21  RPMRALGLRAGHIIQNCFSYHFTPAAFMAEGAAAKLGCAVIPSGIGQTELQVQTMASLRP 80
           R +RA G R G I+Q  + Y        A   A KLGC VIP   GQTE QVQ +   +P
Sbjct: 123 RSIRAAGGRRGDIVQISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIRDFKP 182

Query: 81  DAYVGTPSFLKIIIEKAQEMGV--ALDSLQRALVSAEALPPSLRSWFHDNGVPNVLQLYA 138
              + TPS++  +IE+ Q  G+  A  SL+  +  AE    ++R         + + +Y 
Sbjct: 183 SIIMVTPSYMLNVIEEFQRQGMDPASSSLKVGIFGAEPWTDAMRREIQQRAGLDAVDIYG 242

Query: 139 SADI--GNIAYESISEGKVNPGMVLDEDLILEIVRPGTGDPVRPGEVGEVVVTSFNPD-Y 195
            +++    +A E I E K  P ++ ++    EI+ P TG+ +  G  GE+V TS + +  
Sbjct: 243 LSEVMGPGVASECI-ESKDGP-VIWEDHFYPEIIDPDTGEVLPDGVEGELVFTSLSKEAL 300

Query: 196 PLIRFGTGDLTATLDGVSPCGRTNTRIKGWMGRADQTTKVRGMFVHPSQVAE-ITRRHGL 254
           P+IR+ T DLT  L    P  R+  RI    GR+D    +RG+ V P+Q+ E I +   L
Sbjct: 301 PMIRYRTRDLTRLL---PPTSRSMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKLPQL 357

Query: 255 ILKARLVVSGEMANDSMTLHCETQD 279
             + +L +      DSM++  E ++
Sbjct: 358 APQYQLQIERHGHMDSMSVFAELRE 382


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.135    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,004,514
Number of extensions: 39088
Number of successful extensions: 134
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 131
Number of HSP's successfully gapped: 2
Length of query: 326
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 241
Effective length of database: 1,217,395
Effective search space: 293392195
Effective search space used: 293392195
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 131 (55.1 bits)