BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0004.p (528 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion ... 319 3e-88 gi|300310334|ref|YP_003774426.1| hemolysin activation/secretion ... 91 2e-19 >gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 569 Score = 319 bits (818), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 197/522 (37%), Positives = 292/522 (55%), Gaps = 30/522 (5%) Query: 33 RFEINRFDIAGNTLLPAADVDAAVAPFTGKDRDFSDVKGALVALKAVYQAHGYVLVRIDL 92 RFEI RF +AGNTLLP V AAVAPF+G+ R + D++ AL AL+ +Y++ GY V++ + Sbjct: 52 RFEIRRFVVAGNTLLPQDQVQAAVAPFSGQGRVYGDIQQALEALENLYRSAGYTAVQVHV 111 Query: 93 PEQELNNGVVVLNVVQTRIGNVAIEGNKSFTDTNIRNSLPGLQEGEAPDLNKISKSLKLA 152 PEQEL++G V ++VV+T +G V + N+ F+ NIR S+P L+EG P+L +IS+S++LA Sbjct: 112 PEQELSSGEVRIDVVETVVGKVQVSENRHFSQANIRASIPALKEGVPPNLRRISESVQLA 171 Query: 153 NENPAKKTVMKIKPGAAADEVDASLAVTEESVWLTTLNLDNSGTEQVGRTYAGVVLQNAN 212 N+NPAK+ + + + D++DA + V ++ LNLDN+G+ GR GV +Q++N Sbjct: 172 NDNPAKQVNVTLSAAQSGDQIDAKIEVKDDDPLRVMLNLDNTGSADTGRWRTGVAIQHSN 231 Query: 213 MFGRDHVMTLQYTTTLEHS--GDVAVYGFGYHVPLYALGDSLDFFASHSDVNAGTVTAGI 270 +F RDHV T+ YTT+ + V +Y GY +PLY LGDSLDF S V++G A Sbjct: 232 LFNRDHVATVAYTTSPDSPSGSKVDLYSLGYRLPLYELGDSLDFIYGKSSVSSGQTLALN 291 Query: 271 LDIAVSGKGSMYGARYNQNLTTVGSYESKLVYGIDYKEVRNSMQTLGIELGNDVTVH--- 327 + ++G+G +Y R+N G + SKLV G DYK+V +S G L N T++ Sbjct: 292 STLNITGRGEVYALRWNHFFARNGEWSSKLVLGADYKKVDSSCAING-GLLNGATLNTCL 350 Query: 328 -----PLSVGYVGNWSMQNGVANWGATLSHNVAGGAKGRQQDFDAARVGAK--------- 373 PLS+ Y V + ++ N+A G R DA +V + Sbjct: 351 PYSTLPLSLTYSAQRQGVGEVIDANIGVARNMATGQARRN---DALQVTDRYSLFTNGRQ 407 Query: 374 --ADYTALRFAASTTQGLPQEWLARAIVNGQYTRDALIAGEQYGAGGASSVRGFQEREVS 431 ++ A+R AS + +W R N Q + AL A EQ+G GA++VRGF ER VS Sbjct: 408 SMDNFIAVRGGASWLKAFQNDWQMRLAGNLQLSPHALPAVEQFGLAGATAVRGFTERAVS 467 Query: 432 NDSGVTANVEIYSPPLCRNTDWQ--CRVLAFYDTAYLTRNRALPGE--FRSMAIRSIGVG 487 DSGV N E+Y+P L ++ + R+LAF D N ++ + SIG G Sbjct: 468 ADSGVVLNSELYTPDLLAKSELKGSMRLLAFVDAGRGANNNVTANSQVQSTLTLASIGAG 527 Query: 488 VRLQLRNNVSLQMDYGHVLRAEATVT-QRGDGRLHARLGLSF 528 +R L + +L++D VL ++T QRGD H L F Sbjct: 528 LRYALGRDFNLRLDVARVLLNGNSITEQRGDLNAHLSATLGF 569 >gi|300310334|ref|YP_003774426.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 565 Score = 90.9 bits (224), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 117/479 (24%), Positives = 202/479 (42%), Gaps = 41/479 (8%) Query: 29 PGSLRFEINRFDIAGNTLLPAADVDAAVAPFTGKDRDFSDVKGALVALKAVYQAHGYVLV 88 P S F + I GNT AA + A VA GK +D+ + + YQAHG+ L Sbjct: 69 PPSAPFLVKEIRIVGNTAFDAATLHALVADQEGKTLTLADLSALAARISSYYQAHGFPLA 128 Query: 89 RIDLPEQELNNGVVVLNVVQTRIGNVAIEGNKSFTDTNIRNSLPGLQEGEAPDLNKISKS 148 R +P Q ++ GVVV+ VV+ R G + + + + + L LQ+ + + +S Sbjct: 129 RAIIPAQTISEGVVVIQVVEARYGKIHLNNSSPVNSRLLESILSSLQKDQMVAERSLDRS 188 Query: 149 LKLANENPAKKTVMKIKPGAAADEVDASLAVTEESVWLTTLNLDNSGTEQVGRT------ 202 L L ++ P +KPG A D + T ++V + ++++DN G +GR Sbjct: 189 LLLLSDVPGVGVEAVLKPGEAVGTSDMDVVTTPKAVSIGSMSIDNFGNRYIGRARLSGTA 248 Query: 203 ------YAGVVLQNANMFGRDHVMT---LQYTTTLEHSGDVAVYGFGYHVPLYALGDSLD 253 + G VL +AN+ M+ + Y T L G G Y + Y LGDS+ Sbjct: 249 SLFNPLHHGDVL-SANLVSTGERMSYGRISYDTLLNGQGTHV--GGAYSMVHYKLGDSVR 305 Query: 254 FFASHSDVNAGTVTAGILDIAVSGKGSMYGARYNQNLTTVGSYESKLVYGIDYKEVRNSM 313 +H + ++ + S ++YG ++ Y D K++ + + Sbjct: 306 ALDAHGNALVASLWLK-HPLLRSRDANLYG---------------QIQY--DTKKLEDRI 347 Query: 314 QTLGIELGNDVTVHPLSV-GYVGNWSMQNGVANWGATLSHNVAGGAKGRQQDFDAARVGA 372 G+ + LS+ G + + + GV+ WG + G + DA Sbjct: 348 GATGLRTDRHLDNWVLSLSGNLRDSVLGGGVSAWGMGWTAGRVGYDDAAAEASDALTAQT 407 Query: 373 KADYTALRFAASTTQGLPQEWLARAIVNGQYTRDALIAGEQYGAGGASSVRGFQEREVSN 432 + ++ F S Q L + A + Q++ L + E+ GG SVR + +S Sbjct: 408 RGGFSKWNFNFSRLQFLGRRDSLYANFSVQWSDANLDSAEKMSVGGPYSVRAYDIGAISG 467 Query: 433 DSGVTANVEIYSPPLCRNTDWQCRVLAFYDTAYLTRNRA--LPGEFRSMAIRSIGVGVR 489 D+G ++E+ + +VLAF D+A + NR G+ S+ + GVG+R Sbjct: 468 DTGYLGSIELRH-EMGSMAGGTLQVLAFVDSARVNINRRQWTTGQ-NSVTLSGTGVGMR 524 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,565,422 Number of extensions: 63628 Number of successful extensions: 177 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 173 Number of HSP's successfully gapped: 3 Length of query: 528 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 438 Effective length of database: 1,193,720 Effective search space: 522849360 Effective search space used: 522849360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)