BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0001.p (337 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 503 e-143 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 279 2e-76 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 248 5e-67 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 209 3e-55 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 199 2e-52 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 503 bits (1294), Expect = e-143, Method: Compositional matrix adjust. Identities = 243/293 (82%), Positives = 265/293 (90%) Query: 26 MLQLKDPSLLRQQAYINGAWCNADGGGTHAVNNPATGDELGTVPDMGTDETRRAIEAAQA 85 MLQLKDP+LLRQQA++NG WC+AD G AV+NPA G+ LG VP MG ETRRAIEAA A Sbjct: 1 MLQLKDPALLRQQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANA 60 Query: 86 AWPAWRKKTARERAAILRKWNDLMLANADDLALIMTAEQGKPLAESKGEIAYAASFIEWF 145 AWPAW+KKTA+ER+AILR+W +LMLAN DDLALIMTAEQGKPLAE++GEI YAASFIEWF Sbjct: 61 AWPAWKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWF 120 Query: 146 GEEAKRTYGDTIPSPVPGNRIVVIKEPIGVCAAITPWNFPAAMITRKAGPALAAGCPMVL 205 EE KRTYGDTIPSP P NRIVVIKE IGVCAAITPWNFPAAMITRKAGPALAAGCPMVL Sbjct: 121 AEEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVL 180 Query: 206 KPAQATPFSALALAVLAERAGVPAGVFSVVTGSSKVIGAEMTGNPVVRKLSFTGSTETGK 265 KPA+ATPFSALALAVLAERAG+PAGVFSVVTG+ K IG EMT NP+VRK+SFTGST GK Sbjct: 181 KPAEATPFSALALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGK 240 Query: 266 LLMQQCAATIKKVSLELGGNAPFIVFDDADLDAAVEGAIASKFRNTGQTCVCA 318 LLMQQ A++IKK+SLELGGNAPFIVFDDADLDAAVEGAIASK+RN GQTCVCA Sbjct: 241 LLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCA 293 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 279 bits (713), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 166/297 (55%), Positives = 215/297 (72%), Gaps = 5/297 (1%) Query: 24 LSMLQLKDPSLLRQQAYINGAWCNADGGGTHAVNNPATGDELGTVPDMGTDETRRAIEAA 83 ++ L L+ L+ I W A G V++PATG +VPD G + R A+EAA Sbjct: 1 MTSLTLQRQDLMPGAQLIGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAA 60 Query: 84 QAAWPAWRKKTARERAAILRKWNDLMLANADDLALIMTAEQGKPLAESKGEIAYAASFIE 143 AA+ AWR A++RA I+++WNDL+LA+ DDL +++ EQGKPLAE+KGE+AYAAS++E Sbjct: 61 VAAFAAWRATPAKQRAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVE 120 Query: 144 WFGEEAKRTYGDTIPSPVPGNRIVVIKEPIGVCAAITPWNFPAAMITRKAGPALAAGCPM 203 WFGEEA R GD IP+PV G R++ +KEP+GV AAITPWNFPAAMI RK PALAAGC + Sbjct: 121 WFGEEATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTV 180 Query: 204 VLKPAQATPFSALALAVLAERAGVPAGVFSVVTGSS----KVIGAEMTGNPVVRKLSFTG 259 V KPA+ TP ++LAL LA+ AGVP GV ++VT S +V+ + + VRK+SFTG Sbjct: 181 VCKPAEDTPLTSLALVRLAQEAGVPVGVINIVTASRERTPEVVDVWLA-DGRVRKISFTG 239 Query: 260 STETGKLLMQQCAATIKKVSLELGGNAPFIVFDDADLDAAVEGAIASKFRNTGQTCV 316 ST GK L + A T+KK+SLELGGNAPFIVFDDAD+DAA++G +A+KFRN GQTCV Sbjct: 240 STAVGKHLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCV 296 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 248 bits (633), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 129/280 (46%), Positives = 174/280 (62%) Query: 38 QAYINGAWCNADGGGTHAVNNPATGDELGTVPDMGTDETRRAIEAAQAAWPAWRKKTARE 97 Q +ING W + G T V NPAT + +G + + RA+EAA + WR +A E Sbjct: 6 QLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFE 65 Query: 98 RAAILRKWNDLMLANADDLALIMTAEQGKPLAESKGEIAYAASFIEWFGEEAKRTYGDTI 157 R+ I+R+ DL+ A+++A +MT EQGKPLAE+K E AA I+WF EEA+RTYG + Sbjct: 66 RSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLV 125 Query: 158 PSPVPGNRIVVIKEPIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAQATPFSALA 217 P+ VPG +VIKEP+G AA TPWNFP + RK ALAAGC +++K + TP S Sbjct: 126 PARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAE 185 Query: 218 LAVLAERAGVPAGVFSVVTGSSKVIGAEMTGNPVVRKLSFTGSTETGKLLMQQCAATIKK 277 L AGVPAGV ++V G I + +PV++K+SFTGST GK L +K+ Sbjct: 186 LIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKR 245 Query: 278 VSLELGGNAPFIVFDDADLDAAVEGAIASKFRNTGQTCVC 317 ++ELGG+AP +VFDDAD+D A + +KFRN GQ CV Sbjct: 246 ATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVS 285 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 209 bits (532), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 118/280 (42%), Positives = 168/280 (60%) Query: 38 QAYINGAWCNADGGGTHAVNNPATGDELGTVPDMGTDETRRAIEAAQAAWPAWRKKTARE 97 Q YI+G + +G V +PATGD LG +P + AI+AA A+ WR+ + E Sbjct: 6 QLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQVSPLE 65 Query: 98 RAAILRKWNDLMLANADDLALIMTAEQGKPLAESKGEIAYAASFIEWFGEEAKRTYGDTI 157 R+ ILR+ L A +A +T + GKPLA++ EI AA ++W EE +R YG + Sbjct: 66 RSDILRRAAALARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRLYGRVV 125 Query: 158 PSPVPGNRIVVIKEPIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAQATPFSALA 217 + P R +V++EP+GVCAAI+PWNFP +K A+AAGC +VLK +P + + Sbjct: 126 GARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESPAAIVL 185 Query: 218 LAVLAERAGVPAGVFSVVTGSSKVIGAEMTGNPVVRKLSFTGSTETGKLLMQQCAATIKK 277 +A L AGVPAGVF++V+G S I + +P+VR +SFTGS G L Q A +K+ Sbjct: 186 MAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAALHMKR 245 Query: 278 VSLELGGNAPFIVFDDADLDAAVEGAIASKFRNTGQTCVC 317 +S+ELGG+AP +VFDDA+++ A K RN GQ CV Sbjct: 246 MSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVS 285 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 199 bits (506), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 114/279 (40%), Positives = 161/279 (57%), Gaps = 10/279 (3%) Query: 40 YINGAWCNADGGGTHAVNNPATGDELGTVPDMGTDETRRAIEAAQAAWPAWRKKTARERA 99 YI+G W G AV PAT + + + + +A++AA+ A PAW + ERA Sbjct: 19 YIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATSPLERA 78 Query: 100 AILRKWNDLMLANADDLALIMTAEQGKPLAESKGEIAYAASFIEWFGEEAKRTYGDTI-- 157 A+L + + L+L ++ A +T E G P I+YA + E R D + Sbjct: 79 ALLGRVHALLLERSELFAQALTLEMGAP-------ISYARTAHVPLAAEHLRVARDNLAD 131 Query: 158 -PSPVPGNRIVVIKEPIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAQATPFSAL 216 P P +++EPIGVCA ITPWN+P IT K GPALAAGC +VLKP++ +P SAL Sbjct: 132 YPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSAL 191 Query: 217 ALAVLAERAGVPAGVFSVVTGSSKVIGAEMTGNPVVRKLSFTGSTETGKLLMQQCAATIK 276 A + AGVPAGVF++V GS +GA ++ +P V +S TGST G L+ Q A T+K Sbjct: 192 LFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVK 251 Query: 277 KVSLELGGNAPFIVFDDADLDAAVEGAIASKFRNTGQTC 315 +V+ ELGG +P +V DADL+ A+ +A+ RN GQ+C Sbjct: 252 RVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSC 290 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 981,413 Number of extensions: 41263 Number of successful extensions: 201 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 177 Number of HSP's successfully gapped: 21 Length of query: 337 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 251 Effective length of database: 1,212,660 Effective search space: 304377660 Effective search space used: 304377660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)