BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0110.p (215 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA lig... 345 1e-96 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 112 2e-26 gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid li... 109 2e-25 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 94 8e-21 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 87 8e-19 >gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA ligase [Herbaspirillum seropedicae SmR1] Length = 545 Score = 345 bits (886), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 168/215 (78%), Positives = 186/215 (86%), Gaps = 1/215 (0%) Query: 1 MSETCPILSLAHVPTAELGKEQDVATRVKTGRPLPLVALRTVDADMNDCPHDGKSTGEVV 60 MSETCP+L+LA VPT ELG E++ A RVKTGRPLPLV LRTVD +M DCP DG +TGEVV Sbjct: 331 MSETCPVLTLAQVPTEELGGEREAAIRVKTGRPLPLVDLRTVDGEMQDCPRDGSTTGEVV 390 Query: 61 VRAPWLTQGYLHNPEASQGLWEGGWLHTQDIGNIDARGYLQVTDRIKDVIKTGGEWVSSL 120 VRAPWLTQGYLH EAS+ LW GGWLHTQDIG++DA GYLQVTDRIKDVIKTGGEWVSSL Sbjct: 391 VRAPWLTQGYLHQAEASEELWRGGWLHTQDIGHLDAEGYLQVTDRIKDVIKTGGEWVSSL 450 Query: 121 EIEEIIARHPAVAEIAVIGVKDAKWGERPVALVVLKADQAGKIDADDIRKLVLAAADDGV 180 +IE+IIARHPAVAE AVIG++D KWGERPVALVVLK D A + DDIR+ V AAA+ G Sbjct: 451 QIEDIIARHPAVAETAVIGIQDGKWGERPVALVVLKKD-AAAVSEDDIREHVRAAAEAGA 509 Query: 181 ISKYGVPDRIEFVKELARTSVGKLNKRVMREQYPS 215 IS+YGVPDR+ FV ELARTSVGKLNKRVMREQYPS Sbjct: 510 ISRYGVPDRVRFVAELARTSVGKLNKRVMREQYPS 544 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 112 bits (280), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 60/155 (38%), Positives = 92/155 (59%), Gaps = 7/155 (4%) Query: 56 TGEVVVRAPWLTQGYLHNPEASQGLWEGGWLHTQDIGNIDARGYLQVTDRIKDVIKTGGE 115 TGE+ VR P + GY +NPEA+ ++ W HT D+G++D G+L +T R D+ +GG Sbjct: 398 TGEICVRGPAVFMGYHNNPEANAKAFKHDWFHTGDLGHVDQDGFLYITGRSSDMYISGGS 457 Query: 116 WVSSLEIEEIIARHPAVAEIAVIGVKDAKWGERPVALVVLKADQAGKIDADDIRKLVLAA 175 V EIEE + HPAV+E+AV+GV D KWGE +A++V KA Q ++D + L A Sbjct: 458 NVYPREIEEALLTHPAVSEVAVLGVPDPKWGESGLAVIVCKAGQ--QVDGE-----ALLA 510 Query: 176 ADDGVISKYGVPDRIEFVKELARTSVGKLNKRVMR 210 + I KY P R + + ++ GK+ K+ ++ Sbjct: 511 HLESRIGKYKWPRRFIYWDSMPKSGYGKIVKKQIK 545 >gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid ligase II protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 109 bits (272), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 62/164 (37%), Positives = 98/164 (59%), Gaps = 8/164 (4%) Query: 52 DGKSTGEVVVRAPWLTQGYLHNPEASQGLWEGGWLHTQDIGNIDARGYLQVTDRIKDVIK 111 DG+ GE++ R +GYL N A+ + GGW HT D+G ++A GY+++ DR KD+I Sbjct: 385 DGEEIGEIMFRGNICMKGYLKNDRATHEAFAGGWFHTGDLGVMNADGYIKIKDRSKDIII 444 Query: 112 TGGEWVSSLEIEEIIARHPAVAEIAVIGVKDAKWGERPVALVVLKADQAGKIDADDIRKL 171 +GGE +SS+E+E+++ RHPAV AV+ D KWGE P A V LK + ++ A ++ + Sbjct: 445 SGGENISSVEVEDVLYRHPAVLAAAVVAQPDEKWGETPCAFVELK--EGAEVGAAELTEF 502 Query: 172 VLAAADDGVISKYGVPDRIEFVKELARTSVGKLNKRVMREQYPS 215 + ++ + VP I F L +TS GK+ K +R++ S Sbjct: 503 CRSH-----LAGFKVPKAIYF-GPLPKTSTGKIQKFELRKRMKS 540 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 94.0 bits (232), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 62/183 (33%), Positives = 91/183 (49%), Gaps = 11/183 (6%) Query: 31 GRPLPLVALRTVDADMNDCPHDGKSTGEVVVRAPWLTQGYLHNP-EASQGLWEGGWLHTQ 89 G PLP +D D P GE+ +R P + GY P E ++ + G+ T Sbjct: 384 GLPLPSTEFAILDDDGQPVPLG--QPGEIAIRGPQVMPGYWQRPDETAKVMTPDGFFKTG 441 Query: 90 DIGNIDARGYLQVTDRIKDVIKTGGEWVSSLEIEEIIARHPAVAEIAVIGVKDAKWGERP 149 DIG +D RGY ++ DR KD+I G V E+E ++A+HP V E A +GV D GE Sbjct: 442 DIGVMDERGYTKIVDRKKDMILVSGFNVYPNEVEGVVAQHPGVLECACVGVPDEHTGEA- 500 Query: 150 VALVVLKADQAGKIDADDIRKLVLAAADDGVISKYGVPDRIEFVKELARTSVGKLNKRVM 209 V L V++ D+ + L + Y P IEF EL +T+VGK+ +R + Sbjct: 501 VKLFVVRKDKT-------LSAAQLMDYCKEQFTGYKKPKYIEFRDELPKTNVGKILRREL 553 Query: 210 REQ 212 R++ Sbjct: 554 RDE 556 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 87.4 bits (215), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 61/184 (33%), Positives = 93/184 (50%), Gaps = 8/184 (4%) Query: 31 GRPLPLVALRTVDADMNDCPHDGKSTGEVVVRAP--WLTQGYLHNPEASQGLWEGGWLHT 88 GR +P ++ VD PH + G + +RAP + Y N EA++ + G +L T Sbjct: 370 GRAVPGHVVQIVDEQGQVLPH--GTVGNIAIRAPDPVMFLRYWRNEEATREKFAGDFLLT 427 Query: 89 QDIGNIDARGYLQVTDRIKDVIKTGGEWVSSLEIEEIIARHPAVAEIAVIGVKDAKWGER 148 D+G++D +GY++ R DVI + G + IEE + RHPAV AV+GVKDA E Sbjct: 428 GDLGSMDEQGYIRYLGRNDDVITSAGYRIGPAAIEECLMRHPAVRIAAVVGVKDALRTEV 487 Query: 149 PVALVVLKADQAGKIDADDIRKLVLAAADDGVISKYGVPDRIEFVKELARTSVGKLNKRV 208 A VVLK + D+ K L ++ + P I FV L T+ GK+ ++ Sbjct: 488 VKAFVVLK----DGVTPDEALKAELQQHVRAQLAAHEYPRLISFVAALPTTATGKIMRKT 543 Query: 209 MREQ 212 ++E Sbjct: 544 LKEM 547 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 719,609 Number of extensions: 31072 Number of successful extensions: 120 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 115 Number of HSP's successfully gapped: 11 Length of query: 215 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 134 Effective length of database: 1,236,335 Effective search space: 165668890 Effective search space used: 165668890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)