BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0107.p (389 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312321|ref|YP_003776413.1| protein-glutamate methylesteras... 399 e-112 gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 143 2e-35 gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 143 3e-35 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 126 2e-30 gi|300312675|ref|YP_003776767.1| protein-glutamate methylesteras... 65 1e-11 >gi|300312321|ref|YP_003776413.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 399 bits (1024), Expect = e-112, Method: Compositional matrix adjust. Identities = 196/333 (58%), Positives = 251/333 (75%), Gaps = 4/333 (1%) Query: 1 MKIAIVNDVPMVVEILRRVLAEWPEHQLLWVAQDGAQAVELCAWQLPDLILMDIVMPGVD 60 MKI IVND PM+VE+LRRV+AE H+L+W+A+DG QAV++CAWQLPD++LMD++MP VD Sbjct: 1 MKIGIVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKVD 60 Query: 61 GVEATRRIMQQTPCPILIVTADIGACADKVYQALGHGALDAVDTPALSGSDWRQGSAVLM 120 G EATRRIM+ TPCPIL+VT+D+ ADK+Y+A+G+GALDA TPAL G+ ++ +A L+ Sbjct: 61 GAEATRRIMEATPCPILVVTSDMDTSADKIYEAMGYGALDATQTPALIGAAGKRDAAALI 120 Query: 121 EKISRIGRMERKPVRSPAARVKTEPKTTPLMPALPIASDKLVAIGASAGGPGALATILRQ 180 EKI +GR+ + V SP R P PA + LVA+GASAGGP ALAT+L+ Sbjct: 121 EKIDNLGRLSPEMV-SPPVRGGRAETLAPAQPARADSLPPLVAVGASAGGPAALATLLKA 179 Query: 181 LPDAFPAAIVIVQHIDPGFAGGMAGWLEQQSGLKVKVAQEGDRPQCGTVLMAGTNQHLLF 240 +P F A+VIVQHID FA GMA WL+QQS LKV +AQEG PQ G VL+A +N+HL+ Sbjct: 180 IPQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLAQEGQAPQRGAVLLAASNRHLVV 239 Query: 241 RNRDSLGYVRESPEDVYHPSIDVFFNSVVRQWRGQAVGVLLTGMGRDGAHGAVGLKAMRD 300 + LGYV +P DVY PS+DVFF S+VR W G+ G+LLTGMGRD GA GLKA+RD Sbjct: 240 KEGGRLGYVEGAPNDVYRPSVDVFFQSIVRHWPGKVAGLLLTGMGRD---GAAGLKALRD 296 Query: 301 AGYHTIAQDRESSSVYGMPKAAAALDAATEILP 333 +G HT+AQD++S +VYGMPKAA AL+AA E+LP Sbjct: 297 SGAHTMAQDQKSCAVYGMPKAAVALNAAVEVLP 329 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 143 bits (361), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 114/342 (33%), Positives = 171/342 (50%), Gaps = 38/342 (11%) Query: 14 EILRRVLAEWPEHQLLWVAQDGAQAVELCAWQLPDLILMDIVMPGVDGVEATRRIMQQTP 73 +++++VLA+ P+ +++ A D A+ Q PD+I++DI MP +DGV R+IM P Sbjct: 20 QLMQQVLAQTPDIRVIGTAPDPVFALRKMQQQWPDVIVLDIKMPRMDGVSFLRQIMATRP 79 Query: 74 CPILIVTADIGACADKVYQALGHGALDAVDTPALSGSDWRQGSAVLMEKISRIGRMERKP 133 P+++ +A +G +AL GA+ + P L D+ Q + + R R+ Sbjct: 80 TPVIMCSAHVGPDNALAAEALAAGAVSVIARPQLGVRDFLQQAVEELASAIRAAMQAREG 139 Query: 134 VRSPAA---------------------------RVKTEPKTTPLMP--ALPIASDKLVAI 164 R+P A R + P P L A+ ++VAI Sbjct: 140 QRAPKAPARLGLSPVKAGAREMLHDAAWGAVLQRYTADAVLQPPGPEDGLRPATARIVAI 199 Query: 165 GASAGGPGALATILRQLPDAFPAAIVIVQHIDPGFAGGMAGWLEQQSGLKVKVAQEGDRP 224 GAS GGP AL +L +L P IV+VQH+ F A L + SG +VK A+ DR Sbjct: 200 GASTGGPQALERVLGKLSQRCPG-IVVVQHMPQRFTRSFAERLHRISGAEVKEAEHLDRI 258 Query: 225 QCGTVLMAGTNQHLLFRNRDSLGYVRESPE----DVYHPSIDVFFNSVVRQWRGQAVGVL 280 G VL+A +HL+ R R+ L Y E+ + + PS+DV F S+ + AVG++ Sbjct: 259 LPGRVLIAPGGRHLVVR-REGLQYYVETLDGPLVSRHKPSVDVLFRSLAKAAGSNAVGII 317 Query: 281 LTGMGRDGAHGAVGLKAMRDAGYHTIAQDRESSSVYGMPKAA 322 +TGMG DGA G++ + D G T AQD SS V+GMPK A Sbjct: 318 MTGMGDDGAR---GMRELADCGGATYAQDEASSVVFGMPKEA 356 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 143 bits (360), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 106/343 (30%), Positives = 172/343 (50%), Gaps = 20/343 (5%) Query: 1 MKIAIVNDVPMVVEILRRVLAEWPEHQLLWVAQDGAQAVELCAWQLPDLILMDIVMPGVD 60 +K+ IV+D ++ ++R ++ P+ +++ VA D A EL PD++ +D+ MP +D Sbjct: 3 IKVLIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMD 62 Query: 61 GVEATRRIMQQTPCPILIVTADIGACADKVYQALGHGALDAVDTPALS-GSDWRQGSAVL 119 G++ ++M+ P P+++V++ ++ +AL GA+D V P +S S ++ + ++ Sbjct: 63 GLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMI 122 Query: 120 MEKISRIGRMERKPVRSPAARVKTEPKTTPLMPALP------IASDKLVAIGASAGGPGA 173 +KI K E ALP +S+KL+ IGAS GG A Sbjct: 123 ADKIR----AAAKARVRARPARSAEQAAGGGSEALPQIRNPLTSSEKLIIIGASTGGTEA 178 Query: 174 LATILRQLPDAFPAAIVIVQHIDPGFAGGMAGWLEQQSGLKVKVAQEGDRPQCGTVLMAG 233 + L ++P P I+I QH+ GF A L + V + +R G +A Sbjct: 179 IKEFLIRMPSDCP-GILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERVLPGHAYIAP 237 Query: 234 TNQHLLFRNRDSLGYVRE----SPEDVYHPSIDVFFNSVVRQWRGQAVGVLLTGMGRDGA 289 + HL R Y+ + P + + PS+DV F S AVGV+LTGMG+DGA Sbjct: 238 GHSHLKLV-RSGANYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVGVILTGMGKDGA 296 Query: 290 HGAVGLKAMRDAGYHTIAQDRESSSVYGMPKAAAALDAATEIL 332 G + MR+AG + AQD S V+GMP+ A A+ AA E+L Sbjct: 297 QGML---EMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVL 336 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 126 bits (317), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 110/349 (31%), Positives = 170/349 (48%), Gaps = 23/349 (6%) Query: 1 MKIAIVNDVPMVVEILRRVLAEWPEHQLLWVAQDGAQAVELCAWQLPDLILMDIVMPGVD 60 +K+ I++D ++ L +V + + + ++G +AVEL PD++ +DI MP +D Sbjct: 2 IKLLIIDDSALMRRQLMQVFEQAGGFDI-CLGRNGREAVELNRSFAPDVVTLDINMPEMD 60 Query: 61 GVEATRRIMQQTPCPILIVTADIGACADKVYQALGHGALDAVDTPA----LSGSDWRQGS 116 G+ A +M P P+++V++ A ++A+ GA+D V P+ LS + Q Sbjct: 61 GLSALSLMMLDRPVPVVMVSSLTEQGALATFEAMNLGAVDYVAKPSGTISLSLHEIEQD- 119 Query: 117 AVLMEKISRIGRMERKPVRSPA---ARVKTEPKTTPLMPALPI---ASDKLVAIGASAGG 170 L+ K+ R+ R R+PA AR + EP P+ A ASD LV IG S GG Sbjct: 120 --LLAKV-RVAAKARIRSRAPAPELARAR-EPLAAPVAEAARRGRPASDGLVLIGVSTGG 175 Query: 171 PGALATILRQLPDAFPAAIVIVQHIDPGFAGGMAGWLEQQSGLKVKVAQEGDRPQCGTVL 230 P L IL LP +FP I++ QH+ F A +++ L V Q G V Sbjct: 176 PSTLEEILPALPASFPWPILVAQHMPAAFTRSFAERMDKLCALNVLEVAAPTVVQGGHVY 235 Query: 231 MAGTNQHLLFRNRDSLGYVR---ESPEDVYHPSIDVFFNSVVRQWRG-QAVGVLLTGMGR 286 +A +L R S V ESP ++HPS+++ S ++ Q +GV+LTGMG Sbjct: 236 IARGGADMLVAKRGSHLSVLPKPESPTHLWHPSVELLARSALQSMAARQLIGVMLTGMGN 295 Query: 287 DGAHGAVGLKAMRDAGYHTIAQDRESSSVYGMPKAAAALDAATEILPGA 335 D GA + G TIA+ S V+GMPK A+ +LP + Sbjct: 296 D---GAAAFAEICGDGGRTIAESESSCVVFGMPKDLIDRGGASVVLPAS 341 >gi|300312675|ref|YP_003776767.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 211 Score = 64.7 bits (156), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 63/209 (30%), Positives = 96/209 (45%), Gaps = 9/209 (4%) Query: 149 PLMPALPIASDKLVAIGASAGGPGALATILRQLPDAFPAAIVIVQHIDPGFAGGMAGWLE 208 P + A PI + + IGASAGG AL+ IL L A ++V H+ +A Sbjct: 7 PELAARPIEA---IVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQIFA 63 Query: 209 QQSGLKVKVAQEGDRPQCGTVLMAGTNQHLLF-RNRDS--LGYVRESPEDVYHPSIDVFF 265 + V AQ+ + Q G + A + HLL R+ D L + + P+IDV F Sbjct: 64 DRCRQPVVEAQDKEPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPAIDVLF 123 Query: 266 NSVVRQWRGQAVGVLLTGMGRDGAHGAVGLKAMRDAGYHTIAQDRESSSVYGMPKAAAAL 325 S + + +G+LLTG D GA G++A+ AG T+ Q + + V MP A A+ Sbjct: 124 ESAADVYGPRLLGILLTGGNHD---GAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAM 180 Query: 326 DAATEILPGAVGLKAMRDAGYHTIAQDRE 354 +L +R G +AQ++E Sbjct: 181 GNIDYVLTLQQIADLLRTLGASGLAQEKE 209 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,300,678 Number of extensions: 58876 Number of successful extensions: 353 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 344 Number of HSP's successfully gapped: 7 Length of query: 389 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 302 Effective length of database: 1,207,925 Effective search space: 364793350 Effective search space used: 364793350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)