BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0106.p (453 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312322|ref|YP_003776414.1| two component hybrid sensor his... 685 0.0 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 187 2e-48 gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 174 1e-44 gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 171 9e-44 gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbas... 166 3e-42 >gi|300312322|ref|YP_003776414.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 775 Score = 685 bits (1768), Expect = 0.0, Method: Compositional matrix adjust. Identities = 359/454 (79%), Positives = 397/454 (87%), Gaps = 1/454 (0%) Query: 1 VRDVGHALGKSVRLDIVGHATRIDRDILEKLDAPIGHLLRNAVDHGIESPQERLQAGKPE 60 VRDVG+ LGKSVRLDIVG+ T+IDRDILEKL+APIGHLLRNAVDHGIES +R AGKPE Sbjct: 321 VRDVGNTLGKSVRLDIVGNTTQIDRDILEKLEAPIGHLLRNAVDHGIESSAQRRAAGKPE 380 Query: 61 QGRITLEARHSAGMLQVNVSDDGAGIDMAQLKKAIVARRLSTEDIVARLSEAELLEFLLL 120 QG I LEARHSAGML + V+DDGAGID+ Q++ A+VAR LS ++ RLSEAELLEFLLL Sbjct: 381 QGVIRLEARHSAGMLLIEVADDGAGIDIEQVRSAVVARGLSNQETAERLSEAELLEFLLL 440 Query: 121 PGFSLRDTVTDISGRGVGLDVVADMLKQVRGTVRIASTAGQGTRFQLQLPLTLSVIRSLL 180 P FSLRDTVT+ISGRGVGLDVVADMLKQVRGT+RI + AGQG+RF +QLPLTLSVIRSLL Sbjct: 441 PSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRITTQAGQGSRFLMQLPLTLSVIRSLL 500 Query: 181 VSIGGEPYAFPLAYVNRTLALQREELDMLEGYQHFSFEGRQIGLVSAHQILQKGELRAKG 240 V I GEPYAFPLAYVNRTL L E L LEGYQHFS GRQ+GLVSAHQ+LQKG+ R++ Sbjct: 501 VDIAGEPYAFPLAYVNRTLRLPAEALQTLEGYQHFSHGGRQVGLVSAHQVLQKGQWRSRD 560 Query: 241 ESVMVVVIGDNEHTYGLAVDRFLGERMLVVQALDARLGKIPNVQAGALMEDGAPVLILDV 300 SV VVVIG+ EHTYGLAVD FLGERMLVVQ LD RLGKI +V AGALMEDG P+LILDV Sbjct: 561 GSVCVVVIGEQEHTYGLAVDAFLGERMLVVQPLDPRLGKIADVLAGALMEDGTPLLILDV 620 Query: 301 ADMLRSVEKLSLAGQLENVGQ-ATAEDTGVARKKVLVVDDSLTVRELERKLLSNRGYQVT 359 ADMLR+VEKL+ +G+L+ V Q A T ARKKVLVVDDSLTVRELERKLL+NRGYQVT Sbjct: 621 ADMLRTVEKLTSSGRLQTVDQDGAAPTTTAARKKVLVVDDSLTVRELERKLLTNRGYQVT 680 Query: 360 VAVDGMDGWNAVRTEQFDLVITDIDMPRMDGIELVTLIRQASHLRSLPVMIVSYKDREED 419 VAVDGMDGWNAVR + FDLVITDIDMPRMDGIELV+LIR A++L+SLPVMIVSYKDREED Sbjct: 681 VAVDGMDGWNAVRADSFDLVITDIDMPRMDGIELVSLIRGAANLQSLPVMIVSYKDREED 740 Query: 420 RQRGLEAGADHYFTKSSFHDETLLQAVVDLIGAA 453 RQRGLEAGADHYFTKSSFHDE+LLQAV DLIG A Sbjct: 741 RQRGLEAGADHYFTKSSFHDESLLQAVTDLIGEA 774 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 187 bits (474), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 107/312 (34%), Positives = 173/312 (55%), Gaps = 4/312 (1%) Query: 1 VRDVGHALGKSVRLDIVGHATRIDRDILEKLDAPIGHLLRNAVDHGIESPQERLQAGKPE 60 VRD+ H LGK V G AT +D+ ++E++ P+ HL+RN++DHGIE P R +GK E Sbjct: 383 VRDLAHKLGKQVEFVTHGAATELDKGLIERIVDPLTHLVRNSIDHGIELPDARRASGKSE 442 Query: 61 QGRITLEARHSAGMLQVNVSDDGAGIDMAQLKKAIVARRLSTEDIVARLSEAELLEFLLL 120 G ++L A H G + + V+DDG G++ +K + + + LS+AE+ + + Sbjct: 443 AGTLSLSAEHQGGNIIIEVTDDGGGLNR---EKILAKAKQQGMPVTDNLSDAEVWQLIFE 499 Query: 121 PGFSLRDTVTDISGRGVGLDVVADMLKQVRGTVRIASTAGQGTRFQLQLPLTLSVIRSLL 180 PGFS + VTD+SGRGVG+DVV + + G V I S+ G GT + LPLTL+++ + Sbjct: 500 PGFSTAEQVTDVSGRGVGMDVVKRNILAMGGAVDIRSSKGFGTTISISLPLTLAILDGMS 559 Query: 181 VSIGGEPYAFPLAYVNRTLALQREELDMLEGY-QHFSFEGRQIGLVSAHQILQKGELRAK 239 + IG E Y PL YV +L +++ + G Q G + L+ +QI Sbjct: 560 IRIGEEVYILPLGYVVESLQPLAQDIKEISGQGQVLRVRGEYLPLIPLYQIFNIEPSFTD 619 Query: 240 GESVMVVVIGDNEHTYGLAVDRFLGERMLVVQALDARLGKIPNVQAGALMEDGAPVLILD 299 +VV++ + L VD +G++ +VV+ L++ K+ + ++ DG LI+D Sbjct: 620 PSQGLVVILESDGKKAALFVDDLIGQQQIVVKNLESNYRKVAGISGATILGDGGVALIVD 679 Query: 300 VADMLRSVEKLS 311 VA +LRS +L+ Sbjct: 680 VAALLRSSRQLN 691 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 174 bits (442), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 104/309 (33%), Positives = 181/309 (58%), Gaps = 8/309 (2%) Query: 1 VRDVGHALGKSVRLDIVGHATRIDRDILEKLDAPIGHLLRNAVDHGIESPQERLQAGKPE 60 VRD+ LGK V+L +VG T D++I+E L P+ H++RN++DHG+E P++R +GKP Sbjct: 532 VRDISRKLGKEVQLVLVGEETEADKNIIEALADPLIHIVRNSLDHGLEGPEQRRASGKPA 591 Query: 61 QGRITLEARHSAGMLQVNVSDDGAGIDMAQLKKAIVARRLSTEDIVARLSEAELLEFLLL 120 G +T+ A H + + + DDG GID A +K+ + L E + R+S+ + + + Sbjct: 592 LGSLTISAAHEGDQVVIVIEDDGKGIDPALIKRKAYEKGLIDEAQLERISDQDAVNLVFS 651 Query: 121 PGFSLRDTVTDISGRGVGLDVVADMLKQVRGTVRIASTAGQGTRFQLQLPLTLSVIRSLL 180 GFS + V+D+SGRGVG+DVV + +++V G++ + S +G+GTR Q+ LPL+++V + ++ Sbjct: 652 SGFSTAEVVSDLSGRGVGMDVVRNAVEKVNGSLALHSVSGKGTRLQISLPLSMAVTKVMI 711 Query: 181 VSIGGEPYAFPLAYVNRTLALQREELDMLEGYQHFSFEGRQIGLVSAHQIL---QKGELR 237 + G+ + P+ +V T+ + R L G +GL +QIL + + Sbjct: 712 IESDGQLFGVPMEHVMETVRVPRSSLHFANSSAKMVLRGHILGLKGINQILGIAAEQKTN 771 Query: 238 AKGE-SVMVVVIGDNEHTYGLAVDRFLGERMLVVQALDARLGKIPNVQAG-ALMEDGAPV 295 A GE +V+VV +G + GL +D F E + V+Q + ++ +G ALM DG+ + Sbjct: 772 ADGEVAVLVVRVGG--QSVGLVIDDFR-ETVDVIQKPLLGILGGLSLYSGSALMGDGSVL 828 Query: 296 LILDVADML 304 ++L+ +M Sbjct: 829 MVLNTREMF 837 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 171 bits (434), Expect = 9e-44, Method: Compositional matrix adjust. Identities = 120/342 (35%), Positives = 179/342 (52%), Gaps = 38/342 (11%) Query: 1 VRDVGHALGKSVRLDIVGHATRIDRDILEKLDAPIGHLLRNAVDHGIESPQERLQAGKPE 60 VRD+ LGK + L + G T +D+ ++E+L P+ H++RNA+DHGIE ERL AGKP Sbjct: 407 VRDIARDLGKDIDLVMTGSDTELDKSMVERLADPLMHIVRNAIDHGIEPAAERLAAGKPA 466 Query: 61 QGRITLEARHSAGMLQVNVSDDGAGIDMAQLKKAIVARRLSTEDIVARLSEAELLEFLLL 120 + + L A H +G + + V DDG G+D + VA+ L++ D A LS+AE+ F+ Sbjct: 467 KASLQLNAIHESGSVVIEVMDDGRGMDREAILAKAVAQGLASPD--AELSDAEIYRFVFE 524 Query: 121 PGFSLRDTVTDISGRGVGLDVVADMLKQVRGTVRIASTAGQGTRFQLQLPLTLSVIRSLL 180 PGFS VT++SGRGVG+DVV ++ + G V I + G+GT +L+LPLTL++I Sbjct: 525 PGFSTARQVTELSGRGVGMDVVRRNIEALHGEVSIDTGRGRGTVVRLRLPLTLAIISGFQ 584 Query: 181 VSIGGEPYAFPLAYV------------NRTLALQREELDMLEGYQHFSFEGRQIGLVSAH 228 V +G + PL V + + ++ E L + + F R+ G S Sbjct: 585 VVVGNAVFVIPLDMVVECIDMPPQQPGSHIVNVRDEPLPFVPLRELFDLPARERGRNSL- 643 Query: 229 QILQKGELRAKGESVMVVVIGDNEHTYGLAVDRFLGERMLVVQALDARLGKIPNVQAGAL 288 I+Q G LRA GL VD LGE V++ L GK+ + + Sbjct: 644 VIVQYGPLRA-----------------GLLVDGLLGECQAVIKPLGRLFGKVKGLSGSTI 686 Query: 289 MEDGAPVLILDVADMLRSVEKLSLAGQLENVGQATAEDTGVA 330 + DG LILD+A ++ ++ Q + QA AE GVA Sbjct: 687 LGDGRVALILDIAHLVHHTQQ-----QEQGSAQAYAE-RGVA 722 >gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbaspirillum seropedicae SmR1] Length = 638 Score = 166 bits (421), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 103/275 (37%), Positives = 155/275 (56%), Gaps = 10/275 (3%) Query: 1 VRDVGHALGKSVRLDIVGHATRIDRDILEKLDAPIGHLLRNAVDHGIESPQERLQAGKPE 60 VR++ LGK V L I G T +DR +++ L P+ H+LRN++DHGIE +R+ AGKP Sbjct: 335 VRELAKDLGKDVDLVITGAETEVDRVVVDSLYDPLVHMLRNSLDHGIEV--DRVAAGKPT 392 Query: 61 QGRITLEARHSAGMLQVNVSDDGAGIDMAQLKKAIVARRLSTEDIVARLSEAELLEFLLL 120 + I+L A + + + V DDG G+D L+ +A+ L E L+ E L+ + L Sbjct: 393 KATISLNAWQAGSSVMIEVFDDGKGMDPDMLRSKALAKGLINEH--QALTADEALQLVFL 450 Query: 121 PGFSLRDTVTDISGRGVGLDVVADMLKQVRGTVRIASTAGQGTRFQLQLPLTLSVIRSLL 180 PGFS ++ + +SGRGVG+DVV +++ RGT+RI ST G+GT+F ++LP+ LS+I ++L Sbjct: 451 PGFSTKEVASSVSGRGVGMDVVKTAVEKHRGTIRIESTIGKGTKFSIRLPIELSIIPTML 510 Query: 181 VSIGGEPYAFPLAYVNRTLALQREELDMLEGYQHFSFEGRQIGLVSAHQILQKGELRAKG 240 V G P A P+A V R + L E D + G +GR + + S +L + Sbjct: 511 VRCAGAPLALPMAVVERVVELP-ENFDEVGGAPVLRDQGRPLPVNSLAGVLGYEPGNERV 569 Query: 241 ESVMVVVIGDNEHTYGLAVDRFLGERMLVVQALDA 275 VM V H Y L V+ G LV++ L A Sbjct: 570 GIVMAV-----PHPYILGVESVEGTADLVIKPLTA 599 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,316,902 Number of extensions: 54924 Number of successful extensions: 408 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 388 Number of HSP's successfully gapped: 39 Length of query: 453 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 365 Effective length of database: 1,203,190 Effective search space: 439164350 Effective search space used: 439164350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)