BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0103.p (367 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis... 295 3e-81 gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransf... 89 5e-19 gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransf... 85 9e-18 gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransf... 79 6e-16 gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransfe... 65 6e-12 >gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis proteins protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 295 bits (756), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 160/266 (60%), Positives = 195/266 (73%), Gaps = 5/266 (1%) Query: 15 AVEPIAAMLKQHMGLDIASVGRALIERGVRERMHVLGLADTTGYLDVLQSGVGELQELIE 74 +++ IA LK MGLD+A+VG +LIER VRERM VL + D YL +L S ELQ LIE Sbjct: 3 SLQAIADKLKAAMGLDMATVGASLIERVVRERMAVLEMEDDALYLSLLLSSAAELQALIE 62 Query: 75 LVVVPETWFFRDREAILAVARLARDKIVRAPNATVRILSLPCSTGEEPYSIAMALLEAGV 134 L VVPETWFFRDREAILAVARLA DK+ P A +RILSLPCSTGEEPYS+AMALL+AG+ Sbjct: 63 LAVVPETWFFRDREAILAVARLASDKLAAQPAAVIRILSLPCSTGEEPYSLAMALLDAGL 122 Query: 135 PPAQFRIDGVDICHRSLAAAQRAVYGRNSFRGKQLDYRERHFTYDHEGAGQYDRALAEDV 194 P QF ID DI RSL A +YGRNSFRG++L +R+RHF + GQ+ L E + Sbjct: 123 TPGQFVIDAYDIRTRSLEIAAAGLYGRNSFRGQELAFRDRHFDLEQ---GQWR--LHERI 177 Query: 195 RQQVAFRFGNMLAADFMLNEAPYDFIFCRNVLIYFDREVQLRAVQVLERLLDRDGCIFVG 254 R QV F GN+LA DF+ + PYDFIFCRNVLIYF+R+VQ + VQ+L ++ D IFVG Sbjct: 178 RSQVRFAPGNLLAPDFLRHSQPYDFIFCRNVLIYFERDVQRQVVQLLASVMKPDALIFVG 237 Query: 255 PAEAGLLLKPHIESIGIPLSFGFRRK 280 PAE G+LL P +ES GI L+FGFR++ Sbjct: 238 PAEGGILLGPTMESAGIALAFGFRKR 263 >gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 277 Score = 89.4 bits (220), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 79/245 (32%), Positives = 123/245 (50%), Gaps = 19/245 (7%) Query: 22 MLKQHMGLDIASVGRALIERGVRERMHVLGLADTTGYLDVLQS--GVGELQELIELVVVP 79 + KQ +GL + +AL++ + R++ LGL Y ++ S G E Q I+L+ Sbjct: 24 LFKQQIGLHLPLSKKALLQSRLAGRLNELGLRHLGQYHALISSEAGAQERQRAIDLITTN 83 Query: 80 ETWFFRDREAILAVARLARDKIVRAPNATVRILSLPCSTGEEPYSIAMALLEAGVPPAQF 139 ET+FFR+ V ++ + P +RI S +TGEE Y++AM LL+ P A + Sbjct: 84 ETYFFREPGHFDFVVGQWLPQLAQQPQ--LRIWSAASATGEEAYTLAM-LLDHHRPGASW 140 Query: 140 RIDGVDICHRSLAAAQRAVY----GRNSFRGKQLDYRERHFTYDHEGAGQYDRAL--AED 193 + DI R L A+RA+Y G+N R Y R Y G G+Y+ D Sbjct: 141 SVFASDISTRVLRFARRALYPMVRGQNIPRA----YLRR---YCLMGVGEYEGHFLVQAD 193 Query: 194 VRQQVAFRFGNMLAADFMLNEAPYDFIFCRNVLIYFDREVQLRAVQVLERLLDRDGCIFV 253 +R +V F N+ D ++ YD IF RNV+IYFD E +L+ ++ + L G + V Sbjct: 194 LRAKVQFAQRNLTRLDEH-DDGQYDLIFLRNVIIYFDCETKLQVLRDVIARLRPGGYLVV 252 Query: 254 GPAEA 258 G +E+ Sbjct: 253 GHSES 257 >gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 85.1 bits (209), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 65/237 (27%), Positives = 108/237 (45%), Gaps = 11/237 (4%) Query: 28 GLDIASVGRALIERGVRERMHVLGLADTTGYLDVL--QSGVGELQELIELVVVPETWFFR 85 G+ + R +++ + ER+ G Y +L ++ ELQ L L+ V ET+F R Sbjct: 23 GISFEATKRYFVDKRLIERIEATGSGSFRHYFSLLRFEADGKELQLLTNLMTVNETYFLR 82 Query: 86 DREAILAVARLARDKIVRAPNAT--VRILSLPCSTGEEPYSIAMALLEA--GVPPAQFRI 141 + + +I R +T +RI +PCS+GEE Y++AM L+E G+ I Sbjct: 83 EEYQFQCLVNSMLPEITRNRRSTAPIRIWCIPCSSGEEAYTLAMYLMERWPGIDQWDVEI 142 Query: 142 DGVDICHRSLAAAQRAVYGRNSFRGKQLDYRERHFTYDHEGAGQYDRALAEDVRQQVAFR 201 DI L AQ Y S + D+ ++F + + + + +R V F Sbjct: 143 ISSDIDTGVLRKAQLGRYSARSVQHVPADWMSKYFLRQGD-----EYQICDALRGAVEFT 197 Query: 202 FGNMLAADFMLNEAPYDFIFCRNVLIYFDREVQLRAVQVLERLLDRDGCIFVGPAEA 258 N+ + +D IFCRN+LIYFD + A + L+ +G I +G +E+ Sbjct: 198 RVNLAKSQDTKAYRNFDVIFCRNLLIYFDDVSRKEAAETFYEALNLNGFICLGHSES 254 >gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 290 Score = 79.0 bits (193), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 71/254 (27%), Positives = 110/254 (43%), Gaps = 20/254 (7%) Query: 17 EPIAAMLKQHMGLDIASVGRALIERGVRERMHVLGLADTTGYLDVLQ--SGVGELQELIE 74 E + ++ Q G+ +A + ++ + R+ G+ YLD+L+ E + Sbjct: 24 ERVRGLIYQRAGIALADSKQEMVYSRLARRLRATGITSFQQYLDMLERTHDSEEWEAFTN 83 Query: 75 LVVVPETWFFRDREAILAVARLARDKIVRAPNATVRILSLPCSTGEEPYSIAMALLEAG- 133 + T FFR+ +A K ++ P I STGEEPY+IAM EA Sbjct: 84 ALTTNLTSFFREAHHFPILADFL--KTLKEP---ATIWCSAASTGEEPYTIAMTACEAYG 138 Query: 134 --VPPAQFRIDGVDICHRSLAAAQRAVYGRNSFRGKQLDYRERHFTY---DHEGAGQYDR 188 PP Q I DI LA VY + ++ F HEG Y R Sbjct: 139 SLTPPVQ--IVATDIDTNVLATGSNGVYPMERIEKMSPERVKKFFLRGKGSHEG---YVR 193 Query: 189 ALAEDVRQQVAFRFGNMLAADFMLNEAPYDFIFCRNVLIYFDREVQLRAVQVLERLLDRD 248 E +R+ + F+ N+LA + L+ +D IFCRNV+IYFD+ Q + + L+ Sbjct: 194 VRPE-LRKLITFKQLNLLADGWPLS-GQFDVIFCRNVMIYFDKPTQSKILSRFVPLMKPH 251 Query: 249 GCIFVGPAEAGLLL 262 +F G +E L + Sbjct: 252 ALLFAGHSENFLYV 265 >gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransferase chemotaxis response regulator protein [Herbaspirillum seropedicae SmR1] Length = 283 Score = 65.5 bits (158), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 61/205 (29%), Positives = 94/205 (45%), Gaps = 39/205 (19%) Query: 72 LIELVVVPETWFFRDREAILAVARLARDKIVRAPNA--TVRILSLPCSTGEEPYSIAMAL 129 L+E + V + FRD LA+ R+K+V +++I CS+GEE YS+A+ L Sbjct: 74 LMEYLTVQVSEMFRDPSYFLAL----REKVVPLLRTYPSLKIWVAGCSSGEEVYSLAILL 129 Query: 130 LEAGVPPAQFRIDGVDICHRSLAAAQRAVYGRNSFRGKQLDYRERHFTYDHEGAGQ---- 185 E G+ I DI +L A+ VY G FT +H+ +G Sbjct: 130 REEGLLERSI-IYATDINQSALKKAEAGVYDLERIAG---------FTNNHQKSGARTSL 179 Query: 186 ------------YDRALAEDVRQQVAFRFGNMLAADFMLNEAPYDFIFCRNVLIYFDREV 233 +D++L R+ V F + LA D + E + CRNVLIYF+R++ Sbjct: 180 SDYYTAAYGAVVFDKSL----RKNVVFS-DHSLATDSVFAEMH--LVSCRNVLIYFNRQL 232 Query: 234 QLRAVQVLERLLDRDGCIFVGPAEA 258 Q RA+ + L R G + +G E+ Sbjct: 233 QERALGLFRESLVRKGFLGLGSKES 257 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.140 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,159,254 Number of extensions: 49153 Number of successful extensions: 189 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 181 Number of HSP's successfully gapped: 5 Length of query: 367 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 280 Effective length of database: 1,207,925 Effective search space: 338219000 Effective search space used: 338219000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)