BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0101.p (201 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312326|ref|YP_003776418.1| methyl-accepting chemotaxis pro... 362 e-101 gi|300309873|ref|YP_003773965.1| methyl-accepting chemotaxis tra... 97 1e-21 gi|300310634|ref|YP_003774726.1| methyl-accepting chemotaxis dom... 91 5e-20 gi|300313134|ref|YP_003777226.1| twitching motility transmembran... 90 1e-19 gi|300312448|ref|YP_003776540.1| methyl-accepting chemotaxis tra... 87 6e-19 >gi|300312326|ref|YP_003776418.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 541 Score = 362 bits (928), Expect = e-101, Method: Compositional matrix adjust. Identities = 183/199 (91%), Positives = 194/199 (97%) Query: 1 MEDTMQDVMEAAGSVSGKLAILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGEY 60 MEDTMQ+VM+AAGSVS KLAILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGE+ Sbjct: 341 MEDTMQNVMDAAGSVSAKLAILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGEH 400 Query: 61 GRGFSVVATEIRRLADQTAVATYDIELMVKEIQSAVAAGVMGMDKFSDQVRRGMAEVQQV 120 GRGFSVVATEIRRLADQTAVATYDIE+MVKEIQSAVAAGVMGMDKFSDQVRRGM +VQ Sbjct: 401 GRGFSVVATEIRRLADQTAVATYDIEVMVKEIQSAVAAGVMGMDKFSDQVRRGMQDVQTA 460 Query: 121 GTQLSQIIAQVQALAPRFQVVNEGMQAQANSAEQINQALLQLSEAAQQTVESLQQSSQAI 180 G+QLSQIIAQVQALAPRFQ+VNEGMQAQANSAEQINQAL+QLSEAAQQTVESLQQSSQAI Sbjct: 461 GSQLSQIIAQVQALAPRFQIVNEGMQAQANSAEQINQALVQLSEAAQQTVESLQQSSQAI 520 Query: 181 DELNLVSNGLRNGVSRFKV 199 +EL++VSN LRNGVSRFKV Sbjct: 521 EELHVVSNELRNGVSRFKV 539 >gi|300309873|ref|YP_003773965.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 547 Score = 97.1 bits (240), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 63/172 (36%), Positives = 100/172 (58%), Gaps = 14/172 (8%) Query: 28 NINQVVTTITKVADQTNLLSLNAAIEAEKAGEYGRGFSVVATEIRRLADQTAVATYDIEL 87 ++ +++ I +A QTN+L+LNAA+EA +AGE GRGF+VVA+E+R LA ++AVA +I+ Sbjct: 362 KMSDIISVIEGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAVAAKEIKE 421 Query: 88 MVKEIQSAVAAGVMGMDKFSDQVRRGMAEVQQVGTQLSQIIAQVQALAPRFQVVNEGMQA 147 ++ E S V AG Q+ V G Q++QI+A VQ + Q Sbjct: 422 LIGESMSHVGAG--------SQL------VSDAGQQMAQIVASVQRFGEIMNNIATATQE 467 Query: 148 QANSAEQINQALLQLSEAAQQTVESLQQSSQAIDELNLVSNGLRNGVSRFKV 199 Q + EQIN A++Q+ + QQ ++++S A L+ ++GL VS FK+ Sbjct: 468 QGSGIEQINTAMVQMDQVTQQNAALVEEASAAAQSLSQQASGLLKVVSVFKI 519 >gi|300310634|ref|YP_003774726.1| methyl-accepting chemotaxis domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 568 Score = 91.3 bits (225), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 60/189 (31%), Positives = 101/189 (53%), Gaps = 14/189 (7%) Query: 10 EAAGSVSGKLAILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGEYGRGFSVVAT 69 E V + +NE + I ++ I +A QTN+L+LNAA+EA +AGE GRGF+VVAT Sbjct: 383 EVVARVVDTMGAINESSKRIVDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAT 442 Query: 70 EIRRLADQTAVATYDIELMVKEIQSAVAAGVMGMDKFSDQVRRGMAEVQQVGTQLSQIIA 129 E+R LA ++A A +I+ ++ D +V G V + G+ + +++ Sbjct: 443 EVRSLAQRSAAAAKEIKTLI--------------DNSVSKVEAGSTLVNEAGSTMREVVE 488 Query: 130 QVQALAPRFQVVNEGMQAQANSAEQINQALLQLSEAAQQTVESLQQSSQAIDELNLVSNG 189 VQ + ++ Q QA+ EQI QA+ Q+ + QQ ++Q+S A + + + ++ Sbjct: 489 SVQRVTEIITGISNASQEQASGIEQIQQAINQMDQVTQQNAALVEQASAASESMQMQAHK 548 Query: 190 LRNGVSRFK 198 L + V FK Sbjct: 549 LTDLVGAFK 557 >gi|300313134|ref|YP_003777226.1| twitching motility transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 753 Score = 90.1 bits (222), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 70/206 (33%), Positives = 113/206 (54%), Gaps = 20/206 (9%) Query: 5 MQDVMEAAGSVSGKLAILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGEYGRGF 64 M + E SG++ L E + I ++ I+ + ++TN+L+LNAAI+A AGE GRGF Sbjct: 556 MNGIREQIQETSGRITRLGESSQMIGEITELISDITERTNVLALNAAIQAASAGEAGRGF 615 Query: 65 SVVATEIRRLADQTAVATYDIELMVKEIQSAVAAGVMGMDK----------FSDQVRRGM 114 SVVA E++ LA++++ AT +I +V+ IQ+ V+ MDK SD + Sbjct: 616 SVVAEEVQHLAERSSAATREIGALVRMIQADTRDVVVAMDKSTTGVTEGAVLSDAAGSAL 675 Query: 115 AEVQQVGTQLSQIIAQVQALAPRFQVVNEGMQAQANSAEQINQALLQLSEAAQQTVESLQ 174 A+++ V +L+++I Q A + R Q V+ AN Q Q +L ++ +Q+ E Sbjct: 676 ADIRSVSEKLAELI-QGMAGSTREQAVS------ANGVAQQIQGILTENDIIEQSRE--- 725 Query: 175 QSSQAIDELNLVSNGLRNGVSRFKVS 200 Q S +EL + L N VSRF+V+ Sbjct: 726 QVSALYEELWAAARQLENSVSRFRVT 751 >gi|300312448|ref|YP_003776540.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 596 Score = 87.4 bits (215), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 61/180 (33%), Positives = 101/180 (56%), Gaps = 14/180 (7%) Query: 22 LNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGEYGRGFSVVATEIRRLADQTAVA 81 +N+ + I +++ I +A QTN+L+LNAA+EA +AGE GRGF+VVA+E+R LA ++A A Sbjct: 352 INDSSRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAA 411 Query: 82 TYDIELMVKEIQSAVAAGVMGMDKFSDQVRRGMAEVQQVGTQLSQIIAQVQALAPRFQVV 141 KEI+S + D ++V G V+Q G +S+++ VQ + + Sbjct: 412 -------AKEIKSLI-------DDSVEKVEAGSQLVEQAGATMSEVVTSVQRVTDIVGEI 457 Query: 142 NEGMQAQANSAEQINQALLQLSEAAQQTVESLQQSSQAIDELNLVSNGLRNGVSRFKVSA 201 E + Q+ EQ+N+A+ Q+ E QQ +++++ A L + L VS FK+ A Sbjct: 458 AEASREQSTGIEQVNRAITQMDEVTQQNAALVEEAAAAAQSLQAQATNLVGAVSIFKIEA 517 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.311 0.123 0.308 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 442,367 Number of extensions: 14211 Number of successful extensions: 219 Number of sequences better than 1.0e-08: 68 Number of HSP's gapped: 166 Number of HSP's successfully gapped: 69 Length of query: 201 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 120 Effective length of database: 1,236,335 Effective search space: 148360200 Effective search space used: 148360200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 128 (53.9 bits)