BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0081.p (100 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance ... 184 2e-48 gi|300309361|ref|YP_003773453.1| response regulator for cobalt/z... 131 8e-33 gi|300311799|ref|YP_003775891.1| two component heavy metal respo... 122 5e-30 gi|300313896|ref|YP_003777988.1| two component transcription res... 96 7e-22 gi|300312680|ref|YP_003776772.1| two component response regulato... 86 7e-19 >gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 223 Score = 184 bits (466), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 88/100 (88%), Positives = 95/100 (95%) Query: 1 MAILVIEDDPKTGDYLKKGLRESGYTVDLARTGTDGLHMAMEQNYDLVVLDVMLPGTDGW 60 MAILVIEDDPKTGDYL+KGLRESGY VDLAR G DGLHMA+EQ+YDLVVLDVMLPG DGW Sbjct: 1 MAILVIEDDPKTGDYLRKGLRESGYAVDLARNGADGLHMALEQDYDLVVLDVMLPGLDGW 60 Query: 61 QVMSALRAKRDLPVIFLTARDHVDDRIRGLELGADDYLXE 100 QVMSALR+KRDLPV+FLTARDHVDDRIRGL+LGADDYL + Sbjct: 61 QVMSALRSKRDLPVLFLTARDHVDDRIRGLQLGADDYLVK 100 >gi|300309361|ref|YP_003773453.1| response regulator for cobalt/zinc/cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 131 bits (330), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 60/100 (60%), Positives = 79/100 (79%) Query: 1 MAILVIEDDPKTGDYLKKGLRESGYTVDLARTGTDGLHMAMEQNYDLVVLDVMLPGTDGW 60 M IL++ED+PK+GDYL++GL ESG+ VD +TG DGLH A +Y L++LDVMLPG +GW Sbjct: 1 MHILLVEDEPKSGDYLRRGLTESGFVVDWVQTGVDGLHNARTLDYKLIILDVMLPGMNGW 60 Query: 61 QVMSALRAKRDLPVIFLTARDHVDDRIRGLELGADDYLXE 100 ++ LR D PV+FLTARD V+DR++GLELGADDYL + Sbjct: 61 DILRELRRTHDTPVLFLTARDEVEDRVKGLELGADDYLVK 100 >gi|300311799|ref|YP_003775891.1| two component heavy metal response transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 122 bits (306), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 59/98 (60%), Positives = 74/98 (75%) Query: 1 MAILVIEDDPKTGDYLKKGLRESGYTVDLARTGTDGLHMAMEQNYDLVVLDVMLPGTDGW 60 M IL++ED+ K +YL KGL E G VDLA G DG H+A+E +YD++VLDVMLPG DG+ Sbjct: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDLAANGVDGQHLAIEHDYDVIVLDVMLPGLDGF 60 Query: 61 QVMSALRAKRDLPVIFLTARDHVDDRIRGLELGADDYL 98 V+ ALR ++ PVI LTARD V+DRI+GL GADDYL Sbjct: 61 SVLRALRKVKETPVIMLTARDSVEDRIKGLHDGADDYL 98 >gi|300313896|ref|YP_003777988.1| two component transcription response regulator protein [Herbaspirillum seropedicae SmR1] Length = 244 Score = 95.5 bits (236), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 46/96 (47%), Positives = 63/96 (65%) Query: 3 ILVIEDDPKTGDYLKKGLRESGYTVDLARTGTDGLHMAMEQNYDLVVLDVMLPGTDGWQV 62 IL++ED+PK + L+K L +GY+ G L Q DL++LD+MLPG DGW++ Sbjct: 18 ILIVEDEPKLAELLQKYLALAGYSARHVARGDAALDAVRAQRPDLILLDIMLPGMDGWEI 77 Query: 63 MSALRAKRDLPVIFLTARDHVDDRIRGLELGADDYL 98 LR D+PV+ LTAR +DR+RGLELGADDY+ Sbjct: 78 CRQLRTFSDVPVLMLTARAEEEDRLRGLELGADDYI 113 >gi|300312680|ref|YP_003776772.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 220 Score = 85.5 bits (210), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 46/101 (45%), Positives = 63/101 (62%), Gaps = 3/101 (2%) Query: 1 MAILVIEDDPKTGDYLKKGLRESGYTVDLARTGTDGLHMAMEQNYDLVVLDVMLPGTDGW 60 M +L++EDDP D L + L G+TV G D L N ++VLD+ LPG DG+ Sbjct: 1 MNVLLVEDDPVLTDGLSRVLNSHGFTVHTVNNGNDALSQRF--NAAVLVLDIGLPGIDGF 58 Query: 61 QVMSALRAK-RDLPVIFLTARDHVDDRIRGLELGADDYLXE 100 +V+ +RA + PV+ LTARD + DR+ GLELGADDYL + Sbjct: 59 EVLRRMRAAGNNTPVLLLTARDTIPDRVHGLELGADDYLVK 99 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.141 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 334,639 Number of extensions: 13107 Number of successful extensions: 158 Number of sequences better than 1.0e-08: 29 Number of HSP's gapped: 158 Number of HSP's successfully gapped: 29 Length of query: 100 Length of database: 1,619,870 Length adjustment: 69 Effective length of query: 31 Effective length of database: 1,293,155 Effective search space: 40087805 Effective search space used: 40087805 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 123 (52.0 bits)