BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0079.p (388 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance ... 370 e-103 gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 238 8e-64 gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 191 8e-50 gi|300310593|ref|YP_003774685.1| phosphate regulon two-component... 105 9e-24 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 96 6e-21 >gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 370 bits (950), Expect = e-103, Method: Compositional matrix adjust. Identities = 185/275 (67%), Positives = 216/275 (78%), Gaps = 2/275 (0%) Query: 111 PAWRMSRRAAEITPANLSTRLREEDAPAELRQLAGALNAMLDRLENGYEHLSQFSADLAH 170 P MS+RAA+ITP +LS RL +E PAELR++A + NAMLDRLE GY HL+QFS DLAH Sbjct: 201 PLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAMLDRLEAGYAHLAQFSGDLAH 260 Query: 171 EIRTPVNILMGQTQVTLGQRRNVEEYEQLLESNLEELTRLSRIIENILFLAQTDHAQLVV 230 EIRTP+N+LMGQ+QV LGQRR+ EEYEQ+LESN+EEL RLSRI+ENILFLAQ DHA L V Sbjct: 261 EIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRLSRIVENILFLAQADHAALAV 320 Query: 231 DRSAIDLREELQKIADYFEGVAAERDMQFEVDAAPGLACSANLVMWRRAVSNLVVNAVRY 290 +R+A+DL EEL +I DYFEG+A ER M+F+V AA C ANL+MWRR VSNLV+NAVRY Sbjct: 321 ERNALDLAEELGRITDYFEGIADERGMRFDVQAAG--QCQANLIMWRRVVSNLVINAVRY 378 Query: 291 GDAGTAIRISVRPTADGIAIDVANRGMAMTRQQMERMFDRFYRGDKSRSEFTESNGLGLA 350 G A + IRI +G I V NR +T Q M RMFDRFYRGD+SRS FTESNGLGLA Sbjct: 379 GRAESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRFYRGDRSRSAFTESNGLGLA 438 Query: 351 IVKAIMTVHGGTAAVACSADGWIAFSLRFPTRGKI 385 IVKAIM +HGGTAAV C A GWI FSLRFP+ + Sbjct: 439 IVKAIMALHGGTAAVECPAPGWIRFSLRFPSAAAV 473 Score = 179 bits (454), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 84/105 (80%), Positives = 90/105 (85%) Query: 7 WTLAFRVSALFALIACLVVTGLGMYLYTSARQSLEVRADYTLIGRVEHFRNLLHDLYNVE 66 WTL RV+ALFAL+ LVVTGLG YL SA Q+L RAD LIGRVEHFRNL+HDLYNVE Sbjct: 11 WTLTTRVTALFALMTSLVVTGLGFYLDRSAGQALSQRADQALIGRVEHFRNLMHDLYNVE 70 Query: 67 QMEQRPALFESMLGNEQDVRIFRRQGEAPFINMNPDNLTPPAMTP 111 QMEQRPALFESM+GNEQDVRIFRRQGEAPFI NPD+L PPAMTP Sbjct: 71 QMEQRPALFESMMGNEQDVRIFRRQGEAPFIQSNPDHLVPPAMTP 115 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 238 bits (606), Expect = 8e-64, Method: Compositional matrix adjust. Identities = 133/282 (47%), Positives = 183/282 (64%), Gaps = 2/282 (0%) Query: 104 LTPPAMTPAWRMSRRAAEITPANLSTRLREEDAPAELRQLAGALNAMLDRLENGYEHLSQ 163 L + P M+ + ++ L TRL + AP EL ++ + N MLDRL ++ LSQ Sbjct: 188 LVRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEMLDRLHRSFQQLSQ 247 Query: 164 FSADLAHEIRTPVNILMGQTQVTLGQRRNVEEYEQLLESNLEELTRLSRIIENILFLAQT 223 FSADLAH++RTP+N LM QTQV LG+ R ++EY+ LL SN+EE RLSR++E++LFLA+ Sbjct: 248 FSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRMMESMLFLARA 307 Query: 224 DHAQLVVDRSAIDLREELQKIADYFEGVAAERDMQFEVDAAPGLACSANLVMWRRAVSNL 283 ++A +V+ +D+ +EL +IADYFEG+A E ++ V A L A L+ RRAV NL Sbjct: 308 ENAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAGRLQADATLL--RRAVGNL 365 Query: 284 VVNAVRYGDAGTAIRISVRPTADGIAIDVANRGMAMTRQQMERMFDRFYRGDKSRSEFTE 343 V NA+RY A IR+ V DG+ I V+N G + +Q+ER+FDRFYR D SRS Sbjct: 366 VANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRFYRADPSRSSAGG 425 Query: 344 SNGLGLAIVKAIMTVHGGTAAVACSADGWIAFSLRFPTRGKI 385 S+GLGLAIV++IM +HGG A+V S FSLRFP R + Sbjct: 426 SSGLGLAIVRSIMMLHGGQASVTSSPGQLTVFSLRFPGRSAV 467 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 191 bits (485), Expect = 8e-50, Method: Compositional matrix adjust. Identities = 103/266 (38%), Positives = 158/266 (59%), Gaps = 3/266 (1%) Query: 108 AMTPAWRMSRRAAEITPANLSTRLREEDAPAELRQLAGALNAMLDRLENGYEHLSQFSAD 167 + P +MS RA +P LS R+ E P EL LA A N LDR+E+ Y L F+AD Sbjct: 192 GLAPLRQMSDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGALDRIEDAYTQLEAFNAD 251 Query: 168 LAHEIRTPVNILMGQTQVTLGQRRNVEEYEQLLESNLEELTRLSRIIENILFLAQTDHAQ 227 +AHE+RTP+ L+G+TQV L + R+ E++ +L+SNLEEL R+ I+ ++LFLA++D + Sbjct: 252 VAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIRSIVNDMLFLARSDQGE 311 Query: 228 LVVDRSAIDLREELQKIADYFEGVAAERDMQFEVDAAPGLACSANLVMWRRAVSNLVVNA 287 R + +E+QK D+FE V + M+ EV +++RA++NL+ NA Sbjct: 312 AATARVPARVADEVQKTVDFFEFVLDDSGMRIEVAGDTEATAMMETALFKRALTNLIQNA 371 Query: 288 VRYGDAGTAIRISVRPTADGIAIDVANRGMAMTRQQMERMFDRFYRGDKSRSEFTES--N 345 +++ G +RI + + + + V+N G + + R+FDRFYR D +R + S + Sbjct: 372 IQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRFYRVDSARQDINGSHGH 431 Query: 346 GLGLAIVKAIMTVHGGTAAVACSADG 371 GLGLAIVKA+ +HGG A A S +G Sbjct: 432 GLGLAIVKAVARMHGGR-AFASSKEG 456 >gi|300310593|ref|YP_003774685.1| phosphate regulon two-component sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 436 Score = 105 bits (261), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 73/227 (32%), Positives = 125/227 (55%), Gaps = 11/227 (4%) Query: 164 FSADLAHEIRTPVNILMGQTQVTLGQRRNVEEYEQLLESNL--EELTRLSRIIENILFLA 221 F A+ +HE+RTP+ ++ G ++ L Q N+EE ++ L E+ R+ +I+++L L Sbjct: 209 FVANASHELRTPLTVINGFLEIALAQP-NLEEGTRMAHLKLMTEQGQRMQNLIDDMLTLT 267 Query: 222 QTDHAQLVVDRSAIDLREELQKIADYFEGVAA-ERDMQFEVDAAPGLACSANLVMWRRAV 280 + + + + +R L++IA+ ++A + ++ +D P L +A + R A Sbjct: 268 RLESIDYPLRTEVLRVRPLLERIAEEGRALSAGKHEIVLTIDG-PDLKGNAEEL--RSAF 324 Query: 281 SNLVVNAVRYGDAGTAIRISVRPTAD--GIAIDVANRGMAMTRQQMERMFDRFYRGDKSR 338 +NLV NAVRY G RIS+R +D G V + G+ ++ + + R+ +RFYR DKSR Sbjct: 325 TNLVTNAVRYTPEGG--RISLRWQSDEQGAHFAVQDTGIGISPEHISRLTERFYRVDKSR 382 Query: 339 SEFTESNGLGLAIVKAIMTVHGGTAAVACSADGWIAFSLRFPTRGKI 385 S T+ GLGLAIV+ ++ H T +A D F++ FP+ I Sbjct: 383 SRETQGTGLGLAIVRHVLLRHHATLEIASVPDHGSTFTVHFPSTQTI 429 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 95.5 bits (236), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 87/346 (25%), Positives = 156/346 (45%), Gaps = 15/346 (4%) Query: 46 YTLIGRVEHFRNLLHDLYNVEQMEQRPALFESMLGNEQDVRIFRRQGEAPFINMNPDNLT 105 Y +G R LL D + + Q P FES++G D+R + Q A I + Sbjct: 128 YPNVGPDARLRPLLQDDVIIGWVAQTP--FESVIG-ALDLRFQQSQFAAQIIIAVVAVML 184 Query: 106 PPAMT---------PAWRMSRRAAEITPANLSTRLREEDAPAELRQLAGALNAMLDRLEN 156 + P R++R + + + R+ E ++ E+ LA N + LE Sbjct: 185 AAFLVVRISGVLTRPLTRVARATHRLASGDYAIRV-EVESGDEVGMLAQDFNQLAMTLER 243 Query: 157 GYEHLSQFSADLAHEIRTPVNILMGQTQVTLGQRRNVEEYEQLLESNLEELTRLSRIIEN 216 + +F AD++HE+RTP+ IL GQ + + ++ Q L S E+ L++++ + Sbjct: 244 NEKLRREFMADVSHELRTPLAILNGQLEAL--EDGVLQPDAQTLRSLKSEVEHLNKLVGD 301 Query: 217 ILFLAQTDHAQLVVDRSAIDLREELQKIADYFEGVAAERDMQFEVDAAPGLACSANLVMW 276 + L+ D L R+ IDL L+ A + ++R M+ + + + Sbjct: 302 LYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSCELPASMPVHGDAARL 361 Query: 277 RRAVSNLVVNAVRYGDAGTAIRISVRPTADGIAIDVANRGMAMTRQQMERMFDRFYRGDK 336 ++ +SNL N++RY DAG +++ + + + + M R+F+RF+R + Sbjct: 362 QQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWHLVFDDSEPGVPPEVMARLFERFFRVEA 421 Query: 337 SRSEFTESNGLGLAIVKAIMTVHGGTAAVACSADGWIAFSLRFPTR 382 SR+ + GLGLAI + I+ H GT + S G + + P R Sbjct: 422 SRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGLRVVVVLPAR 467 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,179,828 Number of extensions: 47139 Number of successful extensions: 287 Number of sequences better than 1.0e-08: 34 Number of HSP's gapped: 241 Number of HSP's successfully gapped: 35 Length of query: 388 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 301 Effective length of database: 1,207,925 Effective search space: 363585425 Effective search space used: 363585425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)