BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0065.p (601 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312366|ref|YP_003776458.1| hypothetical protein Hsero_3062... 488 e-139 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 103 4e-23 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 96 8e-21 gi|300313264|ref|YP_003777356.1| short-chain alcohol dehydrogena... 84 4e-17 gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 83 6e-17 >gi|300312366|ref|YP_003776458.1| hypothetical protein Hsero_3062 [Herbaspirillum seropedicae SmR1] Length = 389 Score = 488 bits (1257), Expect = e-139, Method: Compositional matrix adjust. Identities = 238/384 (61%), Positives = 280/384 (72%), Gaps = 1/384 (0%) Query: 216 SSQAPHATSLALFCKVVDNYGDIGICWRLARQLQTEHGVIVTLWVDDLKSFQRICPEVAT 275 SS A SL LFCKVVDNYGDIGICWRLARQL EHG+ V LWVDDL SF RI P V T Sbjct: 2 SSPASPFRSLDLFCKVVDNYGDIGICWRLARQLAREHGIAVRLWVDDLVSFARIWPSVDT 61 Query: 276 DCETQEIQGVIVRHWRDQDGVFDAADMPDIVIEFFACDIPPGYIKAMSECTQRPAWLNLE 335 TQ+ +GV V+HWR QD ++A+D+PD+VIEFF C++PP Y++AM++ +P W NLE Sbjct: 62 QALTQQREGVTVQHWRGQDDHYEASDIPDVVIEFFGCELPPAYVEAMAQRPLKPLWFNLE 121 Query: 336 GLTAEAWVEGCHTLPSPHPQLPLTKYFFFPGFNQRTGGLQIESGLTARREAFLADPRAAA 395 GL+AE WVEGCHTLPSPH L LTKYFFFPGFN+RTGGL E+ L A+R+AFLA + AA Sbjct: 122 GLSAEPWVEGCHTLPSPHRSLKLTKYFFFPGFNERTGGLSFEADLEAQRQAFLASGQGAA 181 Query: 396 DFLAKFGVTEAETAALKVSLFCYPQAPIADLFAAWRDGDRAITCLVPEGVATEAVQAFLG 455 FLA GVT E KVSLFCY APIA+LFA+W+ +TCLVPEGV AV+AFLG Sbjct: 182 -FLAGLGVTAQEAQGCKVSLFCYDYAPIAELFASWQAAPAPVTCLVPEGVGRAAVEAFLG 240 Query: 456 RSAVAGALATQGALTVRVLPFVPQPDYDKLLWSCDLNFARGEDSFVRAQLAGKPFVWHIY 515 +AG AT+G L+VRVLPFVPQ DYD+LLWSCDLNF RGEDSFVRAQ AGKPFVW+IY Sbjct: 241 APMMAGTAATRGQLSVRVLPFVPQTDYDRLLWSCDLNFVRGEDSFVRAQWAGKPFVWNIY 300 Query: 516 PQDENLHHVKLKAFLAIYKAATPAMQAMMLAWNGACTEQADMATRWPALAAELPQLDDLS 575 QD+NLHH KL AFL IY A+ A+ + AWNGA + D W L ELP L + Sbjct: 301 HQDKNLHHTKLNAFLKIYDPASAAVADLNRAWNGAYPGELDWRANWQDLQGELPALTQAA 360 Query: 576 MEWRQQLLANGDLASNLLKFAASV 599 W ++LANGD NLL+FAA + Sbjct: 361 DRWLHKMLANGDFMDNLLRFAARL 384 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 103 bits (257), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 72/188 (38%), Positives = 100/188 (53%), Gaps = 3/188 (1%) Query: 18 GQVALVTGGGSGIGRAAAIRMARAGASAVVIAGRRQPEGETVAAECRAAGAQSLYVQTDV 77 G+VAL+TG +GIGRA A+ AR GA A+++ RRQ E VAA A G ++ V DV Sbjct: 6 GKVALITGASAGIGRATALLFARHGA-ALILTARRQDALEQVAALICAQGGRASVVAGDV 64 Query: 78 TKEGDVQRLIDTVLTQFGRLDAAFNNAGRQERRASLHAQESAVYDAIFDANVRAVFLCLK 137 + +R + L +FG LD A NNAG L + AN+ + FL + Sbjct: 65 GEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFLGAR 124 Query: 138 YQLPPMAARGNGSIVINTSVSGVRNPNPGLALYSASKAAAISLMRSAAMEYXVVGGRNQA 197 Q+P M ARG G+IV +S G PG+A Y A+KA + L++ A +Y V G R A Sbjct: 125 CQIPAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIR--A 182 Query: 198 DYFLPSDI 205 + LP + Sbjct: 183 NALLPGGV 190 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 95.9 bits (237), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 65/169 (38%), Positives = 96/169 (56%), Gaps = 2/169 (1%) Query: 19 QVALVTGGGSGIGRAAAIRMARAGASAVVIAGRRQPEGETVAAECRAAGAQSLYVQTDVT 78 +VA+VTGGGSG G A AR GA A+V+A + G V E +AAG ++++ + DV+ Sbjct: 6 KVAIVTGGGSGFGEGIAKAYAREGA-AIVVADIGEAGGLRVVEEIKAAGGRAVFAKADVS 64 Query: 79 KEGDVQRLIDTVLTQFGRLDAAFNNAGRQERRASLHAQESAVYDAIFDANVRAVFLCLKY 138 K D+ +L+ T L FG+LD NNAG R + E +D ++ NV+++FL K Sbjct: 65 KRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFLSAKT 124 Query: 139 QLPPMAARGNGSIVINTSVSGVRNPNPGLALYSASKAAAISLMRSAAME 187 +P G G+ + S +G+R P PGL Y+ SK A I+ +S A E Sbjct: 125 FVPYFRQVGGGAFINIASTAGIR-PRPGLTWYNGSKGAVITTSKSMAAE 172 >gi|300313264|ref|YP_003777356.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 84.0 bits (206), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 65/176 (36%), Positives = 86/176 (48%), Gaps = 14/176 (7%) Query: 19 QVALVTGGGSGIGRAAAIRMARAGASAVVIAGRRQPEGETVAAECRAAGAQSLYVQTDVT 78 ++ALVTG GSGIGR + + R G V +AGRRQ E G +L V DVT Sbjct: 9 RIALVTGAGSGIGRQITLALLREG-YGVALAGRRQDALEETVRLAGEDGPNALVVAADVT 67 Query: 79 KEGDVQRLIDTVLTQFGRLDAAFNNAGRQERRASLHAQESAVYDAIFDANVRAVFLC--- 135 V++L +FGRLD FNNAG SL + A D N+ VFLC Sbjct: 68 DPASVKQLFAKTRERFGRLDMLFNNAGIFAPPVSLEELSVEQWKAAVDINLTGVFLCSQE 127 Query: 136 ----LKYQLPPMAARGNGSIVINTSVSGVRNPNPGLALYSASKAAAISLMRSAAME 187 +K Q P RG G I+ N S+S P P A Y+A+K A L ++ +++ Sbjct: 128 AFRIMKDQSP----RG-GRIINNGSIS-AHAPRPFSASYTATKHAITGLTKATSLD 177 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 83.2 bits (204), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 57/168 (33%), Positives = 89/168 (52%), Gaps = 6/168 (3%) Query: 15 DMAGQVALVTGGGSGIGRAAAIRMARAGASAVVIAGRRQPEGETVAAECRAAGAQSLYVQ 74 ++ +V ++TG SG+G A +A GA +V+ RR E + A+ AAG Q++ V Sbjct: 4 NIKDKVVVITGASSGLGETTARHLASLGAK-LVLGARRTERLEKLVADITAAGGQAIAVT 62 Query: 75 TDVTKEGDVQRLIDTVLTQFGRLDAAFNNAGRQER--RASLHAQESAVYDAIFDANVRAV 132 TDV + DV+ L+ FGR+D NNAG A L +E +D + D NV+ V Sbjct: 63 TDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEE---WDRMIDVNVKGV 119 Query: 133 FLCLKYQLPPMAARGNGSIVINTSVSGVRNPNPGLALYSASKAAAISL 180 + LP AA+ +G I+ +SV+G++ +YSA+K A +L Sbjct: 120 LYGVAAALPRFAAQSSGHIINVSSVAGIKVFAGMGTVYSATKFAVRAL 167 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,829,087 Number of extensions: 74977 Number of successful extensions: 303 Number of sequences better than 1.0e-08: 37 Number of HSP's gapped: 286 Number of HSP's successfully gapped: 37 Length of query: 601 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 510 Effective length of database: 1,188,985 Effective search space: 606382350 Effective search space used: 606382350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)