BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0061.p (297 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen... 498 e-142 gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenas... 167 8e-43 gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogen... 127 8e-31 gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum s... 84 2e-17 >gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 498 bits (1281), Expect = e-142, Method: Compositional matrix adjust. Identities = 251/297 (84%), Positives = 275/297 (92%) Query: 1 MSADIVFIGLGNMGLPMAQNLVKAGYSVSGHDLVKASVDKLVESGGVTEADAMAAVAKAK 60 MSA+IVFIGLGNMGLPMAQNLVKAG+SVSGHDLVKASVDKLVE GG TEAD+MAAVAKA Sbjct: 1 MSANIVFIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVAKAD 60 Query: 61 VVITMLPASKHVESIYLGDKGILANIKPGTLLIDCSTIAPESARMVAAAALEKGYTMLDA 120 VVITMLPAS+HVES+YLG+ G+LA+ KPGTLLIDCSTIAPE+AR VAAAA +G+ MLDA Sbjct: 61 VVITMLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDA 120 Query: 121 PVSGGTGGATAGTLTFMVGGTDDGFGLAKPYLEKMGKAIYHAGASGSGQTVKVCNNMLLG 180 PVSGGT GATAGTLTFMVGG+ + F A+PYL+KMGKAIYHAG SGSGQTVKVCNNMLLG Sbjct: 121 PVSGGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNMLLG 180 Query: 181 ILMIGTSEAIRLGIANGMDPKVLSEVMSKSSGRNWTLEVYNPCPGVMENAPASKGYAGGF 240 ILMIGTSEAIRLG+ANGMDPKVLSE+MSKSSGRNWTLEVYNPCPGVME APASKGYAGGF Sbjct: 181 ILMIGTSEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMETAPASKGYAGGF 240 Query: 241 GVDLMLKDLGLAVENALATGSSVPMGALARNLYDIHSKSGSGGLDFSSVFNMLAKAK 297 GVDLMLKDLGLAVENALAT ++P+GA ARNLYDIHS +G+G LDFSS+FNML KA+ Sbjct: 241 GVDLMLKDLGLAVENALATNCAIPLGATARNLYDIHSMNGAGKLDFSSIFNMLGKAQ 297 >gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 298 Score = 167 bits (424), Expect = 8e-43, Method: Compositional matrix adjust. Identities = 100/294 (34%), Positives = 165/294 (56%), Gaps = 8/294 (2%) Query: 1 MSADIVFIGLGNMGLPMAQNLVKAGYSVSGHDLVKASVDKLVESGGVTEADAMAAVAKAK 60 MS ++ IGLG MG +A +L++AG++V D+ K +DK +GGV A+ K Sbjct: 1 MSRNVGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAGKVD 60 Query: 61 VVITMLPASKHVESIYLGDKGILANIKPGTLLIDCSTIAPESARMVAAAALEKGYTMLDA 120 VVIT++ + E++ GD G ++ +KPG+++I +T+AP+ A + +EKG +LDA Sbjct: 61 VVITLVVNAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRLVEKGLLLLDA 120 Query: 121 PVSGGTGGATAGTLTFMVGGTDDGFGLAKPYLEKMGKAIYHAGAS-GSGQTVKVCNNMLL 179 PVSGG A +G +T M G + + + L M +Y G + G G VK+ N +L Sbjct: 121 PVSGGAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLA 180 Query: 180 GILMIGTSEAIRLGIANGMDPKVLSEVMSKSSGRNWTLEVYNPCPGVMENAPASKGYAGG 239 G+ + ++EA+ LG+ G++P L EV++ S+G +W E N P ++ S Y Sbjct: 181 GVHIAASAEAMALGLREGVNPDALYEVITHSAGNSWMFE--NRVPHIL-----SGDYTPL 233 Query: 240 FGVDLMLKDLGLAVENALATGSSVPMGALARNLYDIHSKSGSGGLDFSSVFNML 293 VD+ +KDLGL ++ A + +P+ A A ++ + S +G GG D S+V + Sbjct: 234 SAVDIFVKDLGLVLDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIF 287 >gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 127 bits (320), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 92/287 (32%), Positives = 150/287 (52%), Gaps = 8/287 (2%) Query: 8 IGLGNMGLPMAQNLVKAGYSVSGHDLVKASVDKLVESGGVTEADAMAAVAKA-KVVITML 66 IGLGNMG MA +L + GY V G+D +++ L + G + D++AA+A A ++V+ L Sbjct: 7 IGLGNMGRGMAFSLQRGGYEVLGYDAQESTRTALAQQG-LHCVDSIAALAPAVEMVVLSL 65 Query: 67 PASKHVESIYLGDKGILANIKPGTLLIDCSTIAPESARMVAAAALEKGYTMLDAPVSGGT 126 P S VE + G+ G+L + PG +++D +T P S R +AA G ++DAPVSGG Sbjct: 66 PTSDIVEQVVEGEGGLLQHGAPGLVIVDTTTADPNSTRRLAARLQAAGMELVDAPVSGGP 125 Query: 127 GGATAGTLTFMVGGTDDGFGLAKPYLEKMGKAIYHAGASGSGQTVKVCNNMLLGILMIGT 186 GA T+ ++GG+ + +P L M H G G+G K+ NN++ G ++ Sbjct: 126 KGAATATMGMVIGGSAETVARIEPVLAVMSAKRVHVGPVGAGHVAKLLNNLVTGTHLLIA 185 Query: 187 SEAIRLGIANGMDPKVLSEVMSKSSGRNWTLEVYNPCPGVMENAPASKGYAGGFGVDLML 246 EA R+ A G+D + + + ++ S + +EV+ P E + GF + LM Sbjct: 186 GEAARIAAAAGLDARQIFQGLAAGSANSKVIEVFYPTWIFNE------AFDSGFTMKLMR 239 Query: 247 KDLGLAVENALATGSSVPMGALARNLYDIHSKSGSGGLDFSSVFNML 293 KDL LA+ T S+ P+ A L+ + + DF+ + N + Sbjct: 240 KDLRLAMNLIEETRSTTPLAREAGRLWARSVEGLADAEDFNRIVNYI 286 >gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 292 Score = 83.6 bits (205), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 87/289 (30%), Positives = 137/289 (47%), Gaps = 14/289 (4%) Query: 5 IVFIGLGNMGLPMAQNLVKAGYSVSGHDLVKASVDKLVESGGVTEADAMAAVAKAKVVIT 64 I F+GLG MG PMA NLV+AGY+V + A V++L G A A A A V+I+ Sbjct: 3 IAFLGLGTMGFPMAANLVRAGYAVRAWNRSPAPVERLAALGAQAAATPAEAAADADVLIS 62 Query: 65 MLPASKHVESIYLGDKGILANIKPGTLLIDCSTIAPESARMVAAAALEKGYTMLDAPVSG 124 ML E+ L D G LA ++PG + ++ +TI+ A +A +G + APV G Sbjct: 63 MLADDDATET-SLLDAGALAALRPGAIHVNMATISVALALRLAGLHQARGVAYVAAPVLG 121 Query: 125 GTGGATAGTLTFMVGGTDDGFGLAKPYLEKMGKAIYHAGAS-GSGQTVKVCNNMLLGILM 183 A AG L + G + +P L+ +G+ + G K+ N ++ + Sbjct: 122 RVNVAEAGQLNILAAGEEQALAAVQPLLDVLGQKTWRLGRQPEQANAAKLAMNFMIASAI 181 Query: 184 IGTSEAIRLGIANGMDPKVLSEVMSKSSGRNWTLEVYNPCPGVMENAPASKGYAGGFGVD 243 SEA+ L G+D E+ + ++ + VY G + + GF + Sbjct: 182 GAMSEAVALVQGYGLDKAGFIELATSTA---FAAPVYK---GYGQAIADDRFEPAGFKLA 235 Query: 244 LMLKDLGLAVENALATGSSVPMGALARNLYDIHSK---SGSGGLDFSSV 289 L LKD+ LA+E + VP+ +LA L D+H G G LD++++ Sbjct: 236 LGLKDVRLALEA--GEQAHVPL-SLASALRDLHIDGLAHGEGHLDWAAL 281 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.133 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 890,274 Number of extensions: 35494 Number of successful extensions: 145 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 140 Number of HSP's successfully gapped: 4 Length of query: 297 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 212 Effective length of database: 1,217,395 Effective search space: 258087740 Effective search space used: 258087740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 130 (54.7 bits)