BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0060.p (175 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde deh... 316 5e-88 gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde deh... 160 6e-41 gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde deh... 156 8e-40 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 76 1e-15 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 69 1e-13 >gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 316 bits (810), Expect = 5e-88, Method: Compositional matrix adjust. Identities = 153/166 (92%), Positives = 159/166 (95%) Query: 1 VSKAAKERVERLIGVGVEQGAKLLLDGRNCKVPGNESGNFVGPTVFTGVKAGMDIYEQEI 60 VSKAAKERVERLI GVEQGAKLLLDGRNCKV G+E+GNFVGPTVFTGVK MDIY QEI Sbjct: 328 VSKAAKERVERLIASGVEQGAKLLLDGRNCKVAGSENGNFVGPTVFTGVKPEMDIYTQEI 387 Query: 61 FGPAMCIVEVETLDDAIAFINANPNGNGTSIFTSSGWAARKFQNEIDVGQVGINVPIPVP 120 FGPAMCIVE++TLD+AIAFINANPNGNGTSIFTSSG+AARKFQNEIDVGQVGINVPIPVP Sbjct: 388 FGPAMCIVELDTLDEAIAFINANPNGNGTSIFTSSGYAARKFQNEIDVGQVGINVPIPVP 447 Query: 121 VAYFSFTGSRASKLGDLGPNGKQAVLFWTQTKTVTARWYAPDEEAG 166 VAYFSFTGSRASKLGDLGPNGKQAV FWTQTKTVTARWYAPDEEAG Sbjct: 448 VAYFSFTGSRASKLGDLGPNGKQAVTFWTQTKTVTARWYAPDEEAG 493 >gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 503 Score = 160 bits (405), Expect = 6e-41, Method: Compositional matrix adjust. Identities = 78/160 (48%), Positives = 104/160 (65%) Query: 1 VSKAAKERVERLIGVGVEQGAKLLLDGRNCKVPGNESGNFVGPTVFTGVKAGMDIYEQEI 60 V+ A K +VE I GV+ GAKLL+DGR KVPG+E G F+G T+F V M IY++EI Sbjct: 330 VTAAHKAKVEGYIQSGVDAGAKLLVDGRGLKVPGHEEGFFIGGTLFDHVTTEMKIYQEEI 389 Query: 61 FGPAMCIVEVETLDDAIAFINANPNGNGTSIFTSSGWAARKFQNEIDVGQVGINVPIPVP 120 FGP +C+V V A+ INA+ GNG S+FT+ G A +F I VG VGINVPIPVP Sbjct: 390 FGPVLCVVRVPDFASAVQLINAHEYGNGVSLFTADGNTAHEFSRRIQVGMVGINVPIPVP 449 Query: 121 VAYFSFTGSRASKLGDLGPNGKQAVLFWTQTKTVTARWYA 160 +A+ SF G + S GD G++ + F+T+ K++ RW A Sbjct: 450 MAWHSFGGWKRSLFGDTHAYGEEGIRFYTRYKSIMQRWSA 489 >gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 497 Score = 156 bits (395), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 75/158 (47%), Positives = 105/158 (66%) Query: 1 VSKAAKERVERLIGVGVEQGAKLLLDGRNCKVPGNESGNFVGPTVFTGVKAGMDIYEQEI 60 V+ AAKER+E LIG GVE+GA L++DGR +VPG E G FVG T+ V M +Y++EI Sbjct: 324 VTAAAKERIEGLIGRGVEEGATLVVDGRGYQVPGFEKGFFVGGTLLDHVTPEMTVYKEEI 383 Query: 61 FGPAMCIVEVETLDDAIAFINANPNGNGTSIFTSSGWAARKFQNEIDVGQVGINVPIPVP 120 FGP +C++ + A+ INA+ GNG +++T G AR+F +I+VG VG+NVP+PVP Sbjct: 384 FGPVLCVLRCPDIASAVELINAHEYGNGVAVYTRDGGVAREFVRQIEVGMVGVNVPLPVP 443 Query: 121 VAYFSFTGSRASKLGDLGPNGKQAVLFWTQTKTVTARW 158 +A+ SF G + S GD G + V F+T+ K V RW Sbjct: 444 MAFSSFGGWKRSLFGDHHMYGPEGVRFYTRAKAVMQRW 481 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 76.3 bits (186), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 55/153 (35%), Positives = 80/153 (52%), Gaps = 7/153 (4%) Query: 3 KAAKERVERLIGVGVEQGAKLLLDGRNCKVPGNESGNFVGPTVFTGVKAGMDIYEQEIFG 62 KA ERV R I G+++GA LL G V + G FV PT+F GM I +EIFG Sbjct: 334 KAQFERVNRYIQQGLDEGATLLAGG----VSRPDKGWFVQPTIFADANNGMSIAREEIFG 389 Query: 63 PAMCIVEVETLDDAIAFINANPNGNGTSIFTSSGWAARKFQNEIDVGQVGINVPIPVPVA 122 P I+ +T ++AIA N + G +++T++ A + + VG VG+N P+ A Sbjct: 390 PVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAGVKVGAVGVNCWSPLD-A 448 Query: 123 YFSFTGSRASKLGDLGPNGKQAVLFWTQTKTVT 155 + G + S +G G G L +T+ KTVT Sbjct: 449 NLPWGGIKDSGMGREG--GLAGALAYTEEKTVT 479 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 69.3 bits (168), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 52/164 (31%), Positives = 89/164 (54%), Gaps = 9/164 (5%) Query: 2 SKAAKERVERL---IGVGVEQGAKLLLDGRNCKVPGN-ESGNFVGPTVFTGVKAGMDIYE 57 ++A++E++E++ I +G ++GAK+L G ++ G+ SG +V PTVF G M I++ Sbjct: 342 AQASQEQLEKILSYIDIGKQEGAKVLAGGGREELGGDLASGYYVKPTVFQG-NNKMRIFQ 400 Query: 58 QEIFGPAMCIVEVETLDDAIAFINANPNGNGTSIFTSSGWAARKFQNEIDVGQVGINVPI 117 +EIFGP + + + ++A+A N G G ++T G A + EI G+V N Sbjct: 401 EEIFGPVVSVTTFKDEEEALAIANDTLYGLGAGLWTRDGTRAFRMGREIQAGRVWTNCYH 460 Query: 118 PVPVAYFSFTGSRASKLGDLGPNGKQAVLFWTQTKTVTARWYAP 161 P A+ +F G + S +G N K + + QTK + Y+P Sbjct: 461 LYP-AHAAFGGYKQSGIGR--ENHKMMLDHYQQTKNLLVS-YSP 500 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 581,500 Number of extensions: 24970 Number of successful extensions: 84 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 84 Number of HSP's successfully gapped: 12 Length of query: 175 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 96 Effective length of database: 1,245,805 Effective search space: 119597280 Effective search space used: 119597280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)