BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0059.p (332 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde deh... 623 e-180 gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde deh... 297 8e-82 gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde deh... 284 7e-78 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 164 7e-42 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 140 1e-34 >gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 623 bits (1607), Expect = e-180, Method: Compositional matrix adjust. Identities = 298/320 (93%), Positives = 312/320 (97%) Query: 1 MSQANIPNVPLYINGEHVQSTSKEWRDVINPATQEVVARVPFATKEEVDRAVANAKEAFK 60 MSQANIPNVPLY+NGE VQSTSKEWRDV+NPATQEVVARVPFATKEEVDRAVANAKE FK Sbjct: 1 MSQANIPNVPLYLNGEKVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKETFK 60 Query: 61 TWRNTSLAQRMRIMLKFQQLLRDNIAPLAEMITREHGKTLPDAEGEVMRGLEVVEHACSI 120 TWRNTSLAQRMRIMLKFQQLLR+NIAPLAE+ITREHGKTLPDAEGEVMRGLEVVEHACSI Sbjct: 61 TWRNTSLAQRMRIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSI 120 Query: 121 TSLQLGELAENVAGGVDVYTIHQPIGVGAGITAFNFPVMLPCFMFPISVACGNTFVLKPS 180 TSLQLGELAENVAGGVDVYT++QP+GVGAGITAFNFPVMLPCFMFPI+VACGNTFVLKPS Sbjct: 121 TSLQLGELAENVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPS 180 Query: 181 EQDPTSSLFLVELANKAGLPPGVLNVVHGGPEVANMICDHPDIKAVSFIGSTNVGTHIYR 240 EQDPTSSLFLVELAN+AGLPPGVLNVVHGGP+VANMICDHPDIKAVSFIGST+VGTHIYR Sbjct: 181 EQDPTSSLFLVELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYR 240 Query: 241 RASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGKTKE 300 RASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVG+T+E Sbjct: 241 RASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQTRE 300 Query: 301 WIPEIVERSKARAKTPTSAR 320 WIPEIVERSKA P + R Sbjct: 301 WIPEIVERSKAMKVGPGTDR 320 >gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 497 Score = 297 bits (761), Expect = 8e-82, Method: Compositional matrix adjust. Identities = 142/300 (47%), Positives = 195/300 (65%), Gaps = 1/300 (0%) Query: 12 YINGEHVQSTSKEWRDVINPATQEVVARVPFATKEEVDRAVANAKEAFKTWRNTSLAQRM 71 YI G+ V + S + DV NPA VA+V T +EVD AV AF W NT R Sbjct: 7 YIGGQRVDTRSGRYADVFNPAQGVPVAQVALGTSDEVDAAVKAGVAAFPAWSNTPPLTRA 66 Query: 72 RIMLKFQQLLRDNIAPLAEMITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGELAEN 131 R++ +F L++ A +I REHGKT DA+GEV RG+E+VE A I + GE + Sbjct: 67 RVLFRFLHLIQQRADDFARIIVREHGKTFSDAQGEVARGIEIVEFAAGIPQMLKGEYTDQ 126 Query: 132 VAGGVDVYTIHQPIGVGAGITAFNFPVMLPCFMFPISVACGNTFVLKPSEQDPTSSLFLV 191 +A G+D +++ Q +GV AGIT FNFP M+P +MFPI++ACGN FVLKPSE+DP++SL + Sbjct: 127 IARGIDAWSMRQALGVVAGITPFNFPAMVPMWMFPIAIACGNCFVLKPSERDPSASLLIA 186 Query: 192 ELANKAGLPPGVLNVVHGGPEVANMICDHPDIKAVSFIGSTNVGTHIYRRASEAGKRAQC 251 +L +AGLP GV NVV G + + DHP +KA+SF+GST + +IY R S GKR Q Sbjct: 187 DLLKEAGLPDGVFNVVQGDKVTVDALLDHPQVKAISFVGSTPIAEYIYARGSAKGKRVQA 246 Query: 252 MMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGKT-KEWIPEIVERSK 310 + GAKNH +V+PDA A++ L+GAA+G+AG+RCMA SV + VG E + + ER++ Sbjct: 247 LGGAKNHMVVMPDADMQLAVDALMGAAYGSAGERCMAISVALAVGSAGDELVAALAERAR 306 >gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 503 Score = 284 bits (727), Expect = 7e-78, Method: Compositional matrix adjust. Identities = 152/312 (48%), Positives = 213/312 (68%), Gaps = 5/312 (1%) Query: 5 NIPNVPLYINGEHVQSTSKEWRDVINPATQEVVARVPFATKEEVDRAVANAKEAFKTWRN 64 ++P++ +ING+ +S S +DV NPAT A+V A+ +EV+ AVA A A W Sbjct: 2 SLPHIDHFINGQISRSESGRSQDVFNPATGAATAKVALASVQEVNAAVAAASAAAPAWAE 61 Query: 65 TSLAQRMRIMLKFQQLLRDNIAPLAEMITREHGKTLPDAEGEVMRGLEVVEHACSITSLQ 124 T+ +R R++ KF++L+ N LA+ IT EHGK DA+GEV RGLE+VE A I L Sbjct: 62 TAPLKRARVLFKFKELIEQNHDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLL 121 Query: 125 LGELAENVAGGVDVYTIHQPIGVGAGITAFNFPVMLPCFMFPISVACGNTFVLKPSEQDP 184 + +N+ GG+D + + QP+GV AGIT FNFPVM+P +M PI++A GN+FVLKPSE+DP Sbjct: 122 KTQFTDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERDP 181 Query: 185 TSSLFLVELANKAGLPPGVLNVVHGGPEVANMICDHPDIKAVSFIGSTNVGTHIYRRASE 244 + SL L EL +AGLP GV NVV G + + +HP+++AVSF+GST + +IYR+A+E Sbjct: 182 SPSLMLAELFKRAGLPDGVFNVVQGDKVAVDALLEHPEVQAVSFVGSTPIAEYIYRKATE 241 Query: 245 -AGK---RAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGKTKE 300 AG RAQ + GAKNH +V+PDA DQA++ L+GAA+G+AG+RCMA SV V VG + Sbjct: 242 RAGTFPLRAQALGGAKNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVAD 301 Query: 301 WIPE-IVERSKA 311 + E ++ R KA Sbjct: 302 KLVEALIPRVKA 313 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 164 bits (416), Expect = 7e-42, Method: Compositional matrix adjust. Identities = 98/282 (34%), Positives = 148/282 (52%), Gaps = 1/282 (0%) Query: 8 NVPLYINGEHVQSTSKEWRDVINPATQEVVARVPFATKEEVDRAVANAKEAFKTWRNTSL 67 +V L+INGE S S DVINPAT+EV+ ++ A + ++DRA+ A + F+TWRN S Sbjct: 4 DVQLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSA 63 Query: 68 AQRMRIMLKFQQLLRDNIAPLAEMITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGE 127 +R +IM + LLR+ +A ++T E GK L +A+ E + + ++ G Sbjct: 64 FERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGR 123 Query: 128 LAENVAGGVDVYTIHQPIGVGAGITAFNFPVMLPCFMFPISVACGNTFVLKPSEQDPTSS 187 L G I +P+G A T +NFP+ ++A G + ++K E+ P S Sbjct: 124 LVPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASP 183 Query: 188 LFLVELANKAGLPPGVLNVVHGGP-EVANMICDHPDIKAVSFIGSTNVGTHIYRRASEAG 246 L+ AG+P GV+N+V+G P E++ + HP IK +SF GST VG + A + Sbjct: 184 AELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHM 243 Query: 247 KRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMA 288 KRA +G +V DA D A L A F AGQ C++ Sbjct: 244 KRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVS 285 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 140 bits (354), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 91/301 (30%), Positives = 150/301 (49%), Gaps = 2/301 (0%) Query: 12 YINGEHVQSTSKEWRDVINPATQEVVARVPFATKEEVDRAVANAKEAFKTWRNTSLAQRM 71 ++NG+ + E V NPA E++ VP E RA+ A A+ W+ + +R Sbjct: 15 FVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERS 74 Query: 72 RIMLKFQQLLRDNIAPLAEMITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGELAEN 131 I+ ++ +L+ N LA ++T E GK L +A GE+ +E G+ + Sbjct: 75 AILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTIPS 134 Query: 132 VAGGVDVYTIHQPIGVGAGITAFNFPVMLPCFMFPISVACGNTFVLKPSEQDPTSSLFLV 191 + + I + IGV A IT +NFP + ++A G VLKP+E P S+L L Sbjct: 135 PSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALALA 194 Query: 192 ELANKAGLPPGVLNVVHGGPE-VANMICDHPDIKAVSFIGSTNVGTHIYRRASEAGKRAQ 250 LA +AG+P GV +VV G P+ + + +P ++ +SF GST VG + ++++ + K+ Sbjct: 195 VLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKLS 254 Query: 251 CMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCM-ANSVVVLVGKTKEWIPEIVERS 309 +G IV DA D A+ + + + AGQ C+ AN + V G + ++VE Sbjct: 255 LELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAV 314 Query: 310 K 310 K Sbjct: 315 K 315 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 980,956 Number of extensions: 37243 Number of successful extensions: 165 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 142 Number of HSP's successfully gapped: 17 Length of query: 332 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 246 Effective length of database: 1,212,660 Effective search space: 298314360 Effective search space used: 298314360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)