BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0055.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312380|ref|YP_003776472.1| enoyl-CoA hydratase/carnithine ... 396 e-112 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 125 4e-30 gi|300310158|ref|YP_003774250.1| enoyl-CoA hydratase [Herbaspiri... 114 9e-27 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 100 1e-22 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 99 4e-22 >gi|300312380|ref|YP_003776472.1| enoyl-CoA hydratase/carnithine racemase [Herbaspirillum seropedicae SmR1] Length = 269 Score = 396 bits (1017), Expect = e-112, Method: Compositional matrix adjust. Identities = 192/259 (74%), Positives = 219/259 (84%) Query: 1 MSQVADLTIDARGVATITLNRPEVHNAFDDLLIARLIDLIGQARDDPHVNVLVLASTGKS 60 MS A L I RG+ATITLNRPE+HNAFDD LIA LIDL+ QARDD +L+LASTGKS Sbjct: 1 MSSAAHLDITPRGLATITLNRPEIHNAFDDQLIAHLIDLLTQARDDTRTCLLLLASTGKS 60 Query: 61 FSAGADLGWMRRMADATREDNLKDAHQLAALMENLNSFPCPTVARIQGAVMGGGVGLVAC 120 FSAGADL WMRRMADA+REDNL+DA +LA LME LNSFP PT+A++QGAVMGGGVGLV+C Sbjct: 61 FSAGADLAWMRRMADASREDNLRDARKLALLMETLNSFPTPTLAKVQGAVMGGGVGLVSC 120 Query: 121 CDVAIAGDNAFFALSEVKLGLAPAVISPYVIAKIGTSQARRYFVTAERFSAIRAAAIDLV 180 CD+ +A D AFFALSEVKLGLAPA ISPYVIAK+G SQARRYFV+AERF A RAAA+ LV Sbjct: 121 CDIVVASDAAFFALSEVKLGLAPAAISPYVIAKMGVSQARRYFVSAERFPAARAAAMGLV 180 Query: 181 HEVVPATELEAKTEQIIVALLANGPQAMRRAKELAQVANPLPATPALRDYTTGVIADLRA 240 HEV P EL+A+T Q+I LLANGPQAMR AK+L QVANPL TP L +YT GVIADLRA Sbjct: 181 HEVAPPDELDARTAQLIDTLLANGPQAMRAAKQLVQVANPLQVTPELSEYTAGVIADLRA 240 Query: 241 SEEGKEGLRAFLEKESPSW 259 S EG+EGLRAFL+K++P+W Sbjct: 241 SAEGREGLRAFLDKDAPAW 259 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 125 bits (313), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 78/252 (30%), Positives = 131/252 (51%), Gaps = 3/252 (1%) Query: 10 DARGVATITLNRPEVHNAFDDLLIARLIDLIGQARDDPHVNVLVLASTGKSFSAGADLGW 69 D +G+ T+TLNRP+ NA + ++ L + + D V ++LA+ GK+F AG DL Sbjct: 13 DQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANGKAFCAGHDLRQ 72 Query: 70 MRRMADATREDNLKDAHQLAALMENLNSFPCPTVARIQGAVMGGGVGLVACCDVAIAGDN 129 M+ T + L H+ + LM++L P P +A++QG G LVA CD+AIA ++ Sbjct: 73 MQATPQQTYYEAL--FHRCSHLMQSLRELPVPVIAKVQGLATAAGCQLVATCDLAIAAES 130 Query: 130 AFFALSEVKLGLAPAVISPYVIAKIGTSQARRYFVTAERFSAIRAAAIDLVHEVVPATEL 189 A FA+S + +GL + + + + +A VT + A AAA L+++ VP EL Sbjct: 131 ARFAVSGINVGLFCSTPAVALTRNLPIKRAFEMLVTGKFIDAATAAAWGLINQAVPEQEL 190 Query: 190 EAKTEQIIVALLANGPQAMRRAKELAQVANPLPATPALRDYTTGVIADLRASEEGKEGLR 249 ++ T+ + + + ++ R K + + A P + V+A E +EG+ Sbjct: 191 DSATQALAREICSKPAISIARGKAMV-YRQQMMALPDAYAHAAEVMACNIMDPEAQEGIT 249 Query: 250 AFLEKESPSWIA 261 AFL+K P+W A Sbjct: 250 AFLDKRKPNWAA 261 >gi|300310158|ref|YP_003774250.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 281 Score = 114 bits (285), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 77/179 (43%), Positives = 104/179 (58%) Query: 14 VATITLNRPEVHNAFDDLLIARLIDLIGQARDDPHVNVLVLASTGKSFSAGADLGWMRRM 73 V I +NRP N D L +I +G DP V +VLA+ G+SFS G ++ W+R Sbjct: 23 VGWIWINRPYDGNTIDRLTARDIIRAVGVFSKDPEVRAIVLAARGESFSHGIEVQWLRAQ 82 Query: 74 ADATREDNLKDAHQLAALMENLNSFPCPTVARIQGAVMGGGVGLVACCDVAIAGDNAFFA 133 ADA L D +LA L++ L PTVAR+QG G G GL A CD+ IA D+A F Sbjct: 83 ADAPINGALDDTRKLAQLLQVLAECEKPTVARVQGKAAGIGAGLAAACDICIAADHAHFT 142 Query: 134 LSEVKLGLAPAVISPYVIAKIGTSQARRYFVTAERFSAIRAAAIDLVHEVVPATELEAK 192 + + +LG+APAVI+P+++ IG Q +RYF T E +A RA + L HE VP L+ K Sbjct: 143 MGDDRLGMAPAVITPHLVRAIGARQCQRYFQTNELLTAARACELGLAHEAVPVELLDEK 201 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 100 bits (249), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 81/265 (30%), Positives = 126/265 (47%), Gaps = 14/265 (5%) Query: 1 MSQVADLTIDARG-VATITLNRPEVHNAFDDLLIARLIDLIGQARDDPHVNVLVLASTG- 58 M+ +L ++ G A +TL+ P N + +A L L+ + D + LV+ G Sbjct: 1 MTTYTNLKLEKTGHTAVLTLSNPPA-NTWTRAALADLTRLVKELNADRDIYTLVVTGEGE 59 Query: 59 KSFSAGADLGWMRRMADATREDNLKDAHQLAALMENLNSFPCPTVARIQGAVMGGGVGLV 118 K FSAGADL + AD + + A + E L++F ++A I G MGGG+ Sbjct: 60 KFFSAGADL---KLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECA 116 Query: 119 ACCDVAIAGDNAFFALSEVKLGLAPAVIS----PYVIAKIGTSQARRYFVTAERFSAIRA 174 CD+ IA + A AL E +GL P P++ +G A+R + ER +A A Sbjct: 117 LACDIRIAEEQAQMALPEAAVGLLPCAGGTQNLPWL---VGEGWAKRMILCGERVNAETA 173 Query: 175 AAIDLVHEVVPATELEAKTEQIIVALLANGPQAMRRAKELAQVANPLPATPALRDYTTGV 234 I LV EVVP + + + P ++ +K+L Q A P L+ + Sbjct: 174 LRIGLVEEVVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERE-L 232 Query: 235 IADLRASEEGKEGLRAFLEKESPSW 259 DL +++ +EG++AFLEK SP W Sbjct: 233 FVDLFDTQDQREGVQAFLEKRSPQW 257 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 99.0 bits (245), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 78/265 (29%), Positives = 122/265 (46%), Gaps = 5/265 (1%) Query: 1 MSQVADLTIDARGVATITLNRPEVHNAFDDLLIARLIDLIGQARDDPHVNVLVLASTGKS 60 MS + L + G+A ITLNRP+ N+F + L D I + DP V VL+L G+ Sbjct: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 Query: 61 FSAGADLGWMRRMADATREDNLKDA--HQLAALMENLNSFPCPTVARIQGAVMGGGVGLV 118 F AG DLG R + +L ++ L+++L + P P + + G G G L Sbjct: 61 FCAGQDLG-DRAVKPGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLP 119 Query: 119 ACCDVAIAGDNAFFALSEVKLGLAPAVISPYVIAK-IGTSQARRYFVTAERFSAIRAAAI 177 CD+ +A +A F KLGL P Y + + +GT++A + E+ SA +A Sbjct: 120 LACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERW 179 Query: 178 DLVHEVVPATELEAKTEQIIVALLANGPQAMRRAKELAQVANPLPATPALRDYTTGVIAD 237 L+ + V +L + + A A P A + A+P P D + Sbjct: 180 GLIWKCVDDDQL-MEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRA 238 Query: 238 LRASEEGKEGLRAFLEKESPSWIAK 262 L S + +EG+ AFLEK +P + + Sbjct: 239 LGRSRDYREGVTAFLEKRAPQFTGQ 263 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 709,629 Number of extensions: 25665 Number of successful extensions: 109 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 99 Number of HSP's successfully gapped: 8 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)