BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0027.0054.p
         (427 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312381|ref|YP_003776473.1| glutamyl-tRNA synthetase [Herba...   753   0.0  
gi|300312871|ref|YP_003776963.1| glutamyl/glutaminyl-tRNA synthe...    80   2e-16

>gi|300312381|ref|YP_003776473.1| glutamyl-tRNA synthetase
           [Herbaspirillum seropedicae SmR1]
          Length = 482

 Score =  753 bits (1943), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 363/440 (82%), Positives = 387/440 (87%), Gaps = 14/440 (3%)

Query: 1   LFSWAYIY--------------YPNXPPEAVQAIIDGMQWLGLSHDEGPFYQMKRMDRYR 46
           LFSWAY                     PEAVQAII+GMQWLGL HDEGPFYQMKRMDRYR
Sbjct: 43  LFSWAYARRFGGTFVLRIEDTDVERSTPEAVQAIIEGMQWLGLEHDEGPFYQMKRMDRYR 102

Query: 47  EVVAEMLQQGTAYHCYSSPEEVEAMRERQRAAGEKPRYDGTWRPERGKALPAIPEGRKPV 106
           EV+A+ML+QGTAY+CY +PEEVEAMRERQRAAGEKPRYDGTWRPE GK LPAIPEGRKPV
Sbjct: 103 EVIAQMLEQGTAYYCYCTPEEVEAMRERQRAAGEKPRYDGTWRPEPGKTLPAIPEGRKPV 162

Query: 107 VRFRNPLDGDVTWNDVVKGPITISNRELDDLVIARPDGTPTYNFCVVVDDWDMKITHVIR 166
           VRF+NP DG+V+WNDVVKGPITISNRELDDLVIARPDGTPTYNFCVVVDDWDMKITHVIR
Sbjct: 163 VRFKNPQDGEVSWNDVVKGPITISNRELDDLVIARPDGTPTYNFCVVVDDWDMKITHVIR 222

Query: 167 GDDHVNNTPRQINILKALGGALPQYGHVPMILGADGEKLSKRHGAVSVMDYPAQGFLPEA 226
           GDDHVNNTPRQINILKALGG LP+YGHVPMILGADGEKLSKRHGAVSVMDYPAQG+LPEA
Sbjct: 223 GDDHVNNTPRQINILKALGGTLPEYGHVPMILGADGEKLSKRHGAVSVMDYPAQGYLPEA 282

Query: 227 MLNYLARLGWSHGDEEIFSMEQFCGWFDLDHLSKSPAQFNPEKLAWLNNHYIKLADNERL 286
           MLNYLARLGWSHGD+EIFSM+QFC WFDLDHL+K+PAQFNPEKL WLNNHYIKLADN RL
Sbjct: 283 MLNYLARLGWSHGDDEIFSMQQFCAWFDLDHLTKAPAQFNPEKLNWLNNHYIKLADNARL 342

Query: 287 ATLARPLLEKEGAQFDGAPPLAQTIALLKERANTVNELATAVQMFYRQPAPDAALVTQHI 346
           A L RPLLE +GA F+GAP LA  IALLK+RANTVNE+A A  MFYRQPAPDAALV QHI
Sbjct: 343 AGLVRPLLEAQGAVFEGAPDLAAVIALLKDRANTVNEIAAAALMFYRQPAPDAALVAQHI 402

Query: 347 TDAIKPALQDFAARCATVEWNKAALAAMIKEVLAAHALKMPLLAMPLRLIVAGQLQTPAI 406
           T+AIKPAL DFA R ATVEW K A+A MIKEVLAAH +KMP LAMPLRLIVAGQLQTPAI
Sbjct: 403 TEAIKPALADFAQRVATVEWTKEAIAPMIKEVLAAHGIKMPQLAMPLRLIVAGQLQTPAI 462

Query: 407 DAVLELFGRDVVLARLQNYL 426
           DAVL+LFGR+ V ARL  YL
Sbjct: 463 DAVLQLFGREAVQARLSGYL 482


>gi|300312871|ref|YP_003776963.1| glutamyl/glutaminyl-tRNA
           synthetase [Herbaspirillum seropedicae SmR1]
          Length = 294

 Score = 80.5 bits (197), Expect = 2e-16,   Method: Compositional matrix adjust.
 Identities = 79/246 (32%), Positives = 111/246 (45%), Gaps = 23/246 (9%)

Query: 16  AVQAIIDGMQWLGLSHDEGPFYQMKRMDRYREVVAEMLQQGTAYHCYSSPEEVEAMRERQ 75
           A Q II  +  L +  D     Q +R  RY+  +A       AY C  S +E+   R   
Sbjct: 56  AAQDIIATLAALDMRSDGPVLVQSQRKARYQ--LARDRLGALAYPCGCSRKEIADSRVGT 113

Query: 76  RAAGEKPRYDGTWRPERGKALPAIPEGRKPVVRFRNPLDGD----VTWNDVVKGPITIS- 130
            + G    Y GT R   G A    P  +   +R R P  G     V + D   GP     
Sbjct: 114 ASDGAA-LYPGTCR--HGLA----PGKQARTLRLRVPDPGQAGELVQFEDRWLGPQAQHL 166

Query: 131 NRELDDLVIARPDGTPTYNFCVVVDDWDMKITHVIRGDDHVNNTPRQINILKALGGALPQ 190
             E+ D V+ R DG   Y   VVVDD +  +T ++RG D +++T RQI +   LG   P+
Sbjct: 167 AAEVGDFVLQRADGFWAYQLAVVVDDAEQGVTDIVRGADLLDSTARQIYLQGLLGYPTPR 226

Query: 191 YGHVPMILGADGEKLSKRHGAVSV-MDYPAQGFLPEAMLNYLARLGWSHG---DEEIFSM 246
           Y HVP+++   GEK SK++GA ++ ++ P Q     A+    A LG   G   D E F  
Sbjct: 227 YLHVPLLMNETGEKFSKQNGAQALDLNQPLQ-----ALQQAAAFLGLDTGAARDREGFWA 281

Query: 247 EQFCGW 252
               GW
Sbjct: 282 LALTGW 287


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.136    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,485,902
Number of extensions: 65686
Number of successful extensions: 188
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 188
Number of HSP's successfully gapped: 2
Length of query: 427
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 339
Effective length of database: 1,203,190
Effective search space: 407881410
Effective search space used: 407881410
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 132 (55.5 bits)