BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0049.p (257 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC t... 89 3e-19 gi|300309519|ref|YP_003773611.1| branched-chain amino acid ABC t... 89 3e-19 gi|300312237|ref|YP_003776329.1| amino acid ABC transporter perm... 85 6e-18 gi|300312954|ref|YP_003777046.1| amino acid ABC transporter perm... 75 6e-15 gi|300310992|ref|YP_003775084.1| branched-chain aminoacid ABC tr... 72 3e-14 >gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 89.4 bits (220), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 62/204 (30%), Positives = 110/204 (53%), Gaps = 15/204 (7%) Query: 4 DILLQTLASGLLIGLIYALVAIGLTLIFGVMDIVNFAHGEFLMFG-MYSSFWLFALYALD 62 DI +Q + +GL++G +YAL+A+G T+++GV++++NFAHG+ LM G M L + + Sbjct: 2 DIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQVA 61 Query: 63 PIFTLP-LTALFLFGLGA---------LLYKLVIRKITNAPMVSQIFTTFGLMILFRGIA 112 P LP + L + +GA L+ ++ R + NAP ++ + T G+ IL + +A Sbjct: 62 P--GLPGIVQLVIAIVGAIPVCIVVSLLIERIAYRPLRNAPRLAPLITAIGVSILLQTLA 119 Query: 113 QFFWKADFRTIENSMVAGHVSLGGIQIGSPQLTA-GLGAIVVTGVIYLFLTRTKLGAALE 171 W M + V + G I Q+ L + + G++ L + +TK+G A+ Sbjct: 120 MMIWGRSPLPFPQVMPSDPVHIAGALISPTQIMLLALAVLAMVGLV-LIVEKTKMGRAMR 178 Query: 172 ATAADKEAAQLMGIDSQKMFALAW 195 ATA + A LMG+D+ K+ + + Sbjct: 179 ATAENPRIAGLMGVDANKVIVVTF 202 >gi|300309519|ref|YP_003773611.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 89.4 bits (220), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 62/204 (30%), Positives = 110/204 (53%), Gaps = 15/204 (7%) Query: 4 DILLQTLASGLLIGLIYALVAIGLTLIFGVMDIVNFAHGEFLMFG-MYSSFWLFALYALD 62 DI +Q + +GL++G +YAL+A+G T+++GV++++NFAHG+ LM G M L + + Sbjct: 2 DIFIQQIINGLVLGSMYALIALGYTMVYGVLNLINFAHGDILMVGAMVGLSLLKVVQQVA 61 Query: 63 PIFTLP-LTALFLFGLGA---------LLYKLVIRKITNAPMVSQIFTTFGLMILFRGIA 112 P LP + L + +GA L+ ++ R + NAP ++ + T G+ IL + +A Sbjct: 62 P--GLPGIVQLVIAIVGAIPVCIVVSLLIERIAYRPLRNAPRLAPLITAIGVSILLQTLA 119 Query: 113 QFFWKADFRTIENSMVAGHVSLGGIQIGSPQLTA-GLGAIVVTGVIYLFLTRTKLGAALE 171 W M + V + G I Q+ L + + G++ L + +TK+G A+ Sbjct: 120 MMIWGRSPLPFPQVMPSDPVHIAGALISPTQIMLLALAVLAMVGLV-LIVEKTKMGRAMR 178 Query: 172 ATAADKEAAQLMGIDSQKMFALAW 195 ATA + A LMG+D+ K+ + + Sbjct: 179 ATAENPRIAGLMGVDANKVIVVTF 202 >gi|300312237|ref|YP_003776329.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 84.7 bits (208), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 64/193 (33%), Positives = 106/193 (54%), Gaps = 5/193 (2%) Query: 5 ILLQTLASGLLIGLIYALVAIGLTLIFGVMDIVNFAHGEFLMFGMYSSFWLFALYALDPI 64 +L Q L + L +G +YAL A+G TL+FGV+ ++N +HG M G Y++ L AL P+ Sbjct: 1 MLEQQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLLVEQMAL-PL 59 Query: 65 FTLPLTALFLFGLGALLYKLVIRK---ITNAPMVSQIFTTFGLMILFRGIAQ-FFWKADF 120 + L A+ GL L+ +++ K NAP + + T G+ I+ I+Q F + Sbjct: 60 WVALLLAMVATGLLGLIIDVLVLKPLRKRNAPHLIPMIATIGVAIMITNISQGLFGAENK 119 Query: 121 RTIENSMVAGHVSLGGIQIGSPQLTAGLGAIVVTGVIYLFLTRTKLGAALEATAADKEAA 180 R + ++ VSLG + I + Q+ + A V+ V+ + +T+LG AL A A +AA Sbjct: 120 RFPQGTIPEESVSLGNLHITAVQVAIIVIAFVLMVVLLGVMRKTQLGRALRAIAESPKAA 179 Query: 181 QLMGIDSQKMFAL 193 L+GI+ + +F L Sbjct: 180 YLLGINVEGLFLL 192 >gi|300312954|ref|YP_003777046.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 304 Score = 75.1 bits (183), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 64/205 (31%), Positives = 99/205 (48%), Gaps = 20/205 (9%) Query: 4 DILLQTLASGLLIGLIYALVAIGLTLIFGVMDIVNFAHGEFLMF---------GMYSSFW 54 +++ + + + L G++ L+A GLTLIFGVM+ VN AHG F M G SFW Sbjct: 3 NLIFEQVLNSLQFGVMLFLIASGLTLIFGVMNFVNLAHGSFFMIGAYVAAVVAGASGSFW 62 Query: 55 LFALYALDPIFTLPLTALFLFGLGALLYKLVIRKITNAPMVSQIFTTFGLMILFRGIAQF 114 L L A LP TA LG LL +V R++ + Q+ TF +++F + Sbjct: 63 LGLLVA------LPATA----ALGFLLEWVVFRRLYQKDHMVQVLATFAFILIFNDGVRM 112 Query: 115 FWKADFRTIEN-SMVAGHVSLGGIQIGSPQLTAGLGAIVVTGVIYLFLTRTKLGAALEAT 173 W + + AG V+L G+ S +L +VV +Y + RT++G + A Sbjct: 113 IWGPQPLPLNMPAAFAGPVNLFGLPYSSYRLLVMAVGVVVAIALYFLINRTRIGMWVRAG 172 Query: 174 AADKEAAQLMGIDSQKMFALAWGVG 198 A + E MGI+ + +F L + G Sbjct: 173 ANNLEMVTAMGINIRLLFRLVFSFG 197 >gi|300310992|ref|YP_003775084.1| branched-chain aminoacid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 308 Score = 72.4 bits (176), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 60/214 (28%), Positives = 106/214 (49%), Gaps = 28/214 (13%) Query: 6 LLQTLASGLLIGLIYALVAIGLTLIFGVMDIVNFAHGEFLM------------FGMYSSF 53 L Q L +GL +G IYAL+AIG T+++G++ ++NFAHGE M G+ S + Sbjct: 8 LTQQLVNGLSLGAIYALIAIGYTMVYGIIGMINFAHGEIYMIASYVGLVTLTAIGVQSGY 67 Query: 54 WLFALYALDPIFTLPLTALFLFGLGALLYKLVIRKITNAPMVSQIFTTFGLMILFRGIAQ 113 L L I ++ +T L+ G + ++ R + P + + + G+ I + Q Sbjct: 68 PLPLLLGGALIVSVLVTGLY----GWTVERVAYRPLRGGPRLVPLISAIGMSIFLQNYVQ 123 Query: 114 FFWKADFRTIENSMVAGHVSLGGIQIGSP-QLTAGLGAIVVTGV-------IYLFLTRTK 165 A ++ +++G + Q+GS +T +V+ GV + LF+ R++ Sbjct: 124 IGQGARDMSVP-VLISGALEF---QMGSDFTVTIPYSRMVIVGVTLVLMVALTLFIARSR 179 Query: 166 LGAALEATAADKEAAQLMGIDSQKMFALAWGVGA 199 +G A A A D A L+GID+ K+ + + +GA Sbjct: 180 MGRACRACAEDMGMANLLGIDTNKVISFTFVLGA 213 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 622,022 Number of extensions: 19665 Number of successful extensions: 179 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 166 Number of HSP's successfully gapped: 16 Length of query: 257 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 174 Effective length of database: 1,226,865 Effective search space: 213474510 Effective search space used: 213474510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 129 (54.3 bits)