BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0027.0039.p
         (234 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300314008|ref|YP_003778100.1| glycerol-3-phosphate dehydrogen...   168   4e-43
gi|300310299|ref|YP_003774391.1| glycerol-3-phosphate dehydrogen...   155   3e-39

>gi|300314008|ref|YP_003778100.1| glycerol-3-phosphate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 534

 Score =  168 bits (425), Expect = 4e-43,   Method: Compositional matrix adjust.
 Identities = 91/214 (42%), Positives = 124/214 (57%), Gaps = 9/214 (4%)

Query: 12  ISADEEAYLLRAVNQYLEKPIGDADIVWRYAGVRPLFDDESDNPSAVTRDYTLVLQDADN 71
           ISADE AYL +  N+Y EKPI   D+VW Y+GVRPL +D + + SAVTRDY L L +   
Sbjct: 297 ISADETAYLCQLANRYFEKPITPDDVVWSYSGVRPLLEDAAADASAVTRDYQLDL-NLQG 355

Query: 72  TPCVSVYGGKITTYRRLAESVLEKLAPWTKTRRGPWSKDEALPGGKFKRADRA------- 124
            P +S++GGKITT+R+LAE  ++ +AP    +RG W++   LPGG    A  +       
Sbjct: 356 APLLSIFGGKITTFRKLAEEAVDMIAPVLGNQRGAWTEHGCLPGGDLYGAQPSNRSVRQF 415

Query: 125 -QELDYLKKKYAQLDAHFLTRLFGRHGRIAHVILKEAQTPAELGRDFGGGLVQREVDYLI 183
            Q +  L+++Y  L A  L R    +G   H +L+  Q   E+G +   GL   EVDYL+
Sbjct: 416 DQYVQGLQQQYPWLPAALLQRYARAYGSRTHTLLEGRQNVEEMGEEILDGLYAAEVDYLL 475

Query: 184 THEWAYQPDDILWRRTKCGLHMSEQQRAEFAAYF 217
            HEWA    DILWRR+K GLH  +   A   A+ 
Sbjct: 476 RHEWASNAADILWRRSKLGLHFPKGSEAVLDAWL 509


>gi|300310299|ref|YP_003774391.1| glycerol-3-phosphate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 510

 Score =  155 bits (391), Expect = 3e-39,   Method: Compositional matrix adjust.
 Identities = 87/213 (40%), Positives = 113/213 (53%), Gaps = 8/213 (3%)

Query: 12  ISADEEAYLLRAVNQYLEKPIGDADIVWRYAGVRPLFDDESDNPSAVTRDYTLVLQDADN 71
           I A E  YL    N+Y ++ IG  D+VW Y+GVRPL DD ++N SA+TRDY L   DA  
Sbjct: 290 IDAAEIDYLCDMANRYFKRQIGKEDVVWTYSGVRPLLDDSAENASAITRDYLLECDDA-G 348

Query: 72  TPCVSVYGGKITTYRRLAESVLEKLAPWTKTRRGPWSKDEALPGGKFK-------RADRA 124
            P ++V+GGKITTYR+LAE  L  LAP        W+    LPGG  +       R D  
Sbjct: 349 APMLNVWGGKITTYRKLAEEALAMLAPHLGASSQSWTAGAPLPGGDLEQPGKMVARYDFD 408

Query: 125 QELDYLKKKYAQLDAHFLTRLFGRHGRIAHVILKEAQTPAELGRDFGGGLVQREVDYLIT 184
             L   + ++  L A    R    +G     I+ +A + A LG +   GL + E  YL+ 
Sbjct: 409 AYLRKFQARHPWLPAELAHRYARSYGSRVEQIIGQADSLAALGEELSPGLYEAEARYLVQ 468

Query: 185 HEWAYQPDDILWRRTKCGLHMSEQQRAEFAAYF 217
           HEWA   DDILWRR+K GLH   Q R    A+ 
Sbjct: 469 HEWARSADDILWRRSKLGLHCGPQHRGRLEAWL 501


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.135    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 792,209
Number of extensions: 34278
Number of successful extensions: 95
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 91
Number of HSP's successfully gapped: 2
Length of query: 234
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 152
Effective length of database: 1,231,600
Effective search space: 187203200
Effective search space used: 187203200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 129 (54.3 bits)