BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0038.p (350 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312386|ref|YP_003776478.1| sugar ABC transporter ATPase [H... 532 e-152 gi|300312634|ref|YP_003776726.1| sugar ABC transporter ATPase [H... 328 3e-91 gi|300313086|ref|YP_003777178.1| sn-glycerol-3-phosphate ABC tra... 297 9e-82 gi|300312694|ref|YP_003776786.1| sugar ABC transporter ATPase [H... 272 2e-74 gi|300309785|ref|YP_003773877.1| sugar ABC transporter ATPase [H... 270 9e-74 >gi|300312386|ref|YP_003776478.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 349 Score = 532 bits (1370), Expect = e-152, Method: Compositional matrix adjust. Identities = 265/350 (75%), Positives = 298/350 (85%), Gaps = 5/350 (1%) Query: 1 MAAISLKGITKNWGDAQAVKGIDFEVKSGDFTVLLGPSGCGKSTTLRLIAGLDTPSTGAI 60 MAAISL+G+ K+WG+AQAV+ IDFEVK+G FTVLLGPSGCGKSTTLRLIAGLD PS+G+I Sbjct: 1 MAAISLQGVCKHWGEAQAVRNIDFEVKAGQFTVLLGPSGCGKSTTLRLIAGLDKPSSGSI 60 Query: 61 RIGERDVTNLPPAQRKIAMVFQSYALFPHLNVRDNILFGVRVRKEPARHFDERLKRVADI 120 IG RDVT LPPAQRKI+MVFQSYALFPHL+VRDNILFGVRVRKEP ++ RLKRVADI Sbjct: 61 HIGGRDVTQLPPAQRKISMVFQSYALFPHLSVRDNILFGVRVRKEPRAGYETRLKRVADI 120 Query: 121 LGLSHLLDRRPGQLSGGQQQRVALGRAIIADAPVCLMDEPLSNLDAQLRQEMRREIRALQ 180 LGL+HLL+RRP QLSGGQQQRVALGRAIIADAPVCLMDEPLSNLDAQLRQEMRREIRALQ Sbjct: 121 LGLAHLLERRPAQLSGGQQQRVALGRAIIADAPVCLMDEPLSNLDAQLRQEMRREIRALQ 180 Query: 181 QQLNMTMIYVTHDQTEAMSMADQVILLRDGKIEQNAPPAELYAQPASVFAARFIGTPPMN 240 QQLNMTM+YVTHDQTEAMSMADQVILLR+G+IEQ+A PA+LYAQPA+ FAARFIGTPPMN Sbjct: 181 QQLNMTMVYVTHDQTEAMSMADQVILLREGRIEQDAAPADLYAQPATAFAARFIGTPPMN 240 Query: 241 ILPLSNSAAGLVIEGSNGPVIHPHNVDEEWQLGVRPEHIHLV---GQGIAATVDTIEYFG 297 +LPL A GLVI GS+ VI WQLG+RPEHI L + A V+T+EYFG Sbjct: 241 LLPLEPHAEGLVISGSD--VILRDLGQTPWQLGLRPEHIRLADASDSSVPAVVETVEYFG 298 Query: 298 ADTIVGCRVGQASLLMRVPGQLTLVPGDAIQLGWERGNQYFFDRKSGLRR 347 ADTI+G R+G ASLL+R PGQ L GD ++L W+ +QYFFD SGLRR Sbjct: 299 ADTIIGARIGAASLLVRAPGQHRLGAGDPVRLAWDARDQYFFDPASGLRR 348 >gi|300312634|ref|YP_003776726.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 361 Score = 328 bits (842), Expect = 3e-91, Method: Compositional matrix adjust. Identities = 173/355 (48%), Positives = 233/355 (65%), Gaps = 11/355 (3%) Query: 1 MAAISLKGITKNWGDAQAVKGIDFEVKSGDFTVLLGPSGCGKSTTLRLIAGLDTPSTGAI 60 MA++ ++ + K +G Q ++G+D ++ G+FTVL+GPSGCGKST LR++AGL+ + G I Sbjct: 1 MASVQIRAVKKQFGSTQIIRGVDIDIADGEFTVLVGPSGCGKSTLLRMLAGLEEITGGEI 60 Query: 61 RIGERDVTNLPPAQRKIAMVFQSYALFPHLNVRDNILFGVRVRKEPARHFDERLKRVADI 120 IG V N+ P R IAMVFQ+YAL+PH+ VRDN+ F + + K+ DER+K+ ADI Sbjct: 61 LIGGTVVNNVQPKDRDIAMVFQNYALYPHMTVRDNMAFSLTLAKKDKAFVDERVKKAADI 120 Query: 121 LGLSHLLDRRPGQLSGGQQQRVALGRAIIADAPVCLMDEPLSNLDAQLRQEMRREIRALQ 180 LGL+ LLDR P QLSGGQ+QRVA+GRAI+ D V L DEPLSNLDA+LR +MR EI+ L Sbjct: 121 LGLNQLLDRYPRQLSGGQRQRVAMGRAIVRDPQVFLFDEPLSNLDAKLRVQMRTEIKELH 180 Query: 181 QQLNMTMIYVTHDQTEAMSMADQVILLRDGKIEQNAPPAELYAQPASVFAARFIGTPPMN 240 Q+L T IYVTHDQ EAM+MADQ++++RDG +EQ P +LY PA++F A FIG+P MN Sbjct: 181 QRLKTTSIYVTHDQIEAMTMADQIVVMRDGLVEQRGRPLDLYDYPANLFVAGFIGSPAMN 240 Query: 241 ILP--LSNSAAGLVIEGSNGP-VIHPH------NVDEEWQLGVRPEHIHL--VGQGIAAT 289 +P L +A G +E ++G V P+ N ++ GVRPEH+ + GQGIA Sbjct: 241 FIPATLRRNATGAEVEFADGTRVPAPYGAALQGNDGQKVTYGVRPEHLSIGAAGQGIATK 300 Query: 290 VDTIEYFGADTIVGCRVGQASLLMRVPGQLTLVPGDAIQLGWERGNQYFFDRKSG 344 V +E GADT V R G SL + GD I L + + FD +SG Sbjct: 301 VIVVEPTGADTEVFSRFGDTSLTSIFRERHDFGAGDVIHLVPDHSRTHLFDAESG 355 >gi|300313086|ref|YP_003777178.1| sn-glycerol-3-phosphate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 364 Score = 297 bits (761), Expect = 9e-82, Method: Compositional matrix adjust. Identities = 166/364 (45%), Positives = 226/364 (62%), Gaps = 17/364 (4%) Query: 1 MAAISLKGITKNWGDA----QAVKGIDFEVKSGDFTVLLGPSGCGKSTTLRLIAGLDTPS 56 MAAI LK + K +G + GID E+ G+F V++GPSGCGKST LR++AGL+ S Sbjct: 1 MAAIHLKQVRKTYGAGTKAVDVIHGIDAEIADGEFIVMVGPSGCGKSTLLRMVAGLEEIS 60 Query: 57 TGAIRIGERDVTNLPPAQRKIAMVFQSYALFPHLNVRDNILFGVRVRKEPARHFDERLKR 116 +G I IG+R V +L P +R IAMVFQ+YAL+PH+ V N+ +G++++ D R++R Sbjct: 61 SGQIVIGDRVVNDLEPKERDIAMVFQNYALYPHMTVYQNMAYGLKIQGLSKSEIDARVQR 120 Query: 117 VADILGLSHLLDRRPGQLSGGQQQRVALGRAIIADAPVCLMDEPLSNLDAQLRQEMRREI 176 A IL L LL+R P QLSGGQ+QRVA+GRAI+ V L DEPLSNLDA+LR +MR EI Sbjct: 121 AAAILELGALLERTPRQLSGGQRQRVAMGRAIVRKPAVFLFDEPLSNLDAKLRVQMRLEI 180 Query: 177 RALQQQLNMTMIYVTHDQTEAMSMADQVILLRDGKIEQNAPPAELYAQPASVFAARFIGT 236 + L L T +YVTHDQ EAM++ ++I++ G EQ PAE+YA+PA+ F A FIG+ Sbjct: 181 QKLHASLRTTSLYVTHDQVEAMTLGQRMIVMNRGVAEQIGTPAEVYARPATTFVASFIGS 240 Query: 237 PPMNILPLSNSAAGLVIEGSNG--------PVIHPHNVDEEWQLGVRPEHIHLVGQGIAA 288 PPMN+L SA G E S G P +E LGVRPEH+ + G AA Sbjct: 241 PPMNLLQGKLSADGASFEVSKGNASDILRLPQPLTGAAGQERILGVRPEHLLPILDGSAA 300 Query: 289 ----TVDTIEYFGADTIVGCRVGQASLLMRVPGQLTLVPGDAIQLGWERGNQYFFDRKSG 344 V+ +E GA+ +V R G +L++R P + + G I + G+ ++FD KS Sbjct: 301 QLSLEVELVEALGAELLVHARCGGQALVLRCPANVQVRTGQRIGASFGAGDVHWFDVKS- 359 Query: 345 LRRI 348 RRI Sbjct: 360 TRRI 363 >gi|300312694|ref|YP_003776786.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 272 bits (696), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 159/360 (44%), Positives = 219/360 (60%), Gaps = 16/360 (4%) Query: 1 MAAISLKGITKNW-GDAQAVKGIDFEVKSGDFTVLLGPSGCGKSTTLRLIAGLDTPSTGA 59 MA I + + K++ G + +D + G+F VLLGPSGCGKST LR+IAGL+ S G Sbjct: 1 MADIHCQALAKHYAGGPPVLHPLDLHIGDGEFVVLLGPSGCGKSTMLRMIAGLEDISGGT 60 Query: 60 IRIGERDVTNLPPAQRKIAMVFQSYALFPHLNVRDNILFGVRVRKEPARHFDERLKRVAD 119 +RIG V +LP +R +AMVFQ+YAL+PH++V DNI FG+R K PA D R++ VA Sbjct: 61 LRIGGTVVNDLPARERNVAMVFQNYALYPHMSVYDNIAFGLRRLKRPAAEIDRRVREVAA 120 Query: 120 ILGLSHLLDRRPGQLSGGQQQRVALGRAIIADAPVCLMDEPLSNLDAQLRQEMRREIRAL 179 +L L LL+R+P +SGGQQQR A+ RAII V L DEPLSNLDA+LR ++R +I+ L Sbjct: 121 LLNLEALLERKPRAMSGGQQQRAAIARAIIKTPSVFLFDEPLSNLDAKLRAQLRGDIKRL 180 Query: 180 QQQLNMTMIYVTHDQTEAMSMADQVILLRDGKIEQNAPPAELYAQPASVFAARFIGTPPM 239 Q+L T +YVTHDQ EAM++AD+VIL++DG+I Q PAELY P ++FAA FIGTP M Sbjct: 181 HQRLRTTTVYVTHDQLEAMTLADRVILMQDGRIVQAGSPAELYRYPRNLFAAGFIGTPAM 240 Query: 240 NILP--LSNSAAGLVIEGSN------GPVIHPHNVDEEWQLGVRPEHIHLVGQGIAA--- 288 N L + L IE ++ G +L VRP+H+ + G+ A Sbjct: 241 NFLSGTVQRQDGQLFIETAHQRWALTGERFSRLRHAMAVKLAVRPDHVRIAGEREPAASL 300 Query: 289 ----TVDTIEYFGADTIVGCRVGQASLLMRVPGQLTLVPGDAIQLGWERGNQYFFDRKSG 344 +V+ +E GAD ++ R G +L VP PG + L ++ + FD +SG Sbjct: 301 TCPVSVELVEILGADALLTTRCGDQTLTALVPADRLPQPGATLTLALDQHELHVFDVESG 360 >gi|300309785|ref|YP_003773877.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 372 Score = 270 bits (691), Expect = 9e-74, Method: Compositional matrix adjust. Identities = 147/356 (41%), Positives = 215/356 (60%), Gaps = 21/356 (5%) Query: 1 MAAISLKGITKNWGDAQAVKGIDFEVKSGDFTVLLGPSGCGKSTTLRLIAGLDTPSTGAI 60 MAA+S++ + K + D + ++ I+ E++ G+F V +GPSGCGKST LR+IAGL+ S G + Sbjct: 1 MAAVSIRNLAKRYDDNEVMRDINLEIEDGEFVVFVGPSGCGKSTLLRMIAGLEEISDGDL 60 Query: 61 RIGERDVTNLPPAQRKIAMVFQSYALFPHLNVRDNILFGVRVRKEPARHFDERLKRVADI 120 IG R + +P ++R +AMVFQSYAL+PH+++ DN+ FG+++ + D ++ A I Sbjct: 61 DIGARRMNEVPASKRGVAMVFQSYALYPHMSLYDNMAFGLKIAGKSKAEIDAAVQHAAKI 120 Query: 121 LGLSHLLDRRPGQLSGGQQQRVALGRAIIADAPVCLMDEPLSNLDAQLRQEMRREIRALQ 180 L + HLLDR+P LSGGQ+QRVA+GRAI V L DEPLSNLDA LR +MR E L Sbjct: 121 LHIDHLLDRKPRALSGGQRQRVAIGRAITRQPSVFLFDEPLSNLDAALRVKMRLEFAKLH 180 Query: 181 QQLNMTMIYVTHDQTEAMSMADQVILLRDGKIEQNAPPAELYAQPASVFAARFIGTPPMN 240 L TMIYVTHDQ EAM++AD++++L +G+IEQ P +LY PA+ F A FIG+P MN Sbjct: 181 DDLKTTMIYVTHDQIEAMTLADKIVVLSEGRIEQVGSPQQLYHHPANRFVAGFIGSPKMN 240 Query: 241 ILPLSNSA------------AGLVIEGSNGPVIHPHNVDEEWQLGVRPEHIHLVGQGIAA 288 + + +A GL +G + V ++ LGVRPEH++ + QG AA Sbjct: 241 FIDGTVAAIQADGVQVQLPGGGLQWAAVDGSTL---QVGQKVTLGVRPEHLN-IAQGQAA 296 Query: 289 T---VDTIEYFGADTIVGCRV--GQASLLMRVPGQLTLVPGDAIQLGWERGNQYFF 339 +E G + + + +L++RVP L G + L + + F Sbjct: 297 LQARCTALELLGDFSYLYAAYEGSEDALILRVPDSLDAPHGSVLPLAADPARCHLF 352 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,165,718 Number of extensions: 52067 Number of successful extensions: 591 Number of sequences better than 1.0e-08: 133 Number of HSP's gapped: 351 Number of HSP's successfully gapped: 145 Length of query: 350 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 264 Effective length of database: 1,212,660 Effective search space: 320142240 Effective search space used: 320142240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)